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The map label for this gene is nifF [H]

Identifier: 71907115

GI number: 71907115

Start: 1601649

End: 1602182

Strand: Reverse

Name: nifF [H]

Synonym: Daro_1483

Alternate gene names: 71907115

Gene position: 1602182-1601649 (Counterclockwise)

Preceding gene: 71907116

Following gene: 71907114

Centisome position: 35.6

GC content: 63.86

Gene sequence:

>534_bases
ATGGCCAAAATCGGGATTTTTTTCGGCACCGACACCGGGCGGACCCGGCTGATCGCCAAGCAGATTGCAAAGAAGCTGGG
TGACGCTGCCGACGTGCCGGTCAATATCGGCCGGGTCACCCTGGCCGACTTTCTCGCCTACGACGCGCTGATCGTCGGTA
GCCCGACGCTGGGCGACGGGGAATTGCCCGGCCAGTCGGTCGGCCTCAACCAGCCGAGCTGGGAGGAATTCCTGCCGCAA
CTGGCCGACGCCGATCTGACCGGCAAGACTGTGGCCATCTTCGGCCTCGGCGACCAGAAGAAATACGCCGAAGAATTTGT
CGACGCCATCCGCCTGCTCCACGATGCCTTCGCCGATGCAGGAGCCCGCATGGTCGGGCGCTGGCCAACCGAGGGTTACG
AATTCAAGGCATCGCAGGCCGTCGATGGCGACGAATTCGTCGGCCTCGCGCTCGACCAGCACCACCAGCCGGTACTGACC
GAAGAGCGCATCGATCGCTGGCTGGCCCAGATCACTCCGGAGTTGCTGCCATGA

Upstream 100 bases:

>100_bases
GCAATTCGAGAATGGTCCGGATGGCAACCTCGGTAACCCGGTCGGCTGCCTGCCAGAAGAATTGACGCAGGAAGAAGCGA
TCAGCGCCTGAGGAAAAGCC

Downstream 100 bases:

>100_bases
ACGCCAATCCCTGGTCGCTACCCAAAGACCGCGCCCTGCGCCGCCTGCTCGTCTCGCTCGACGAGCGCGCTGGCCAGGCC
TGCGATGTCGCCCCCGACGA

Product: flavodoxin FldA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 177; Mature: 176

Protein sequence:

>177_residues
MAKIGIFFGTDTGRTRLIAKQIAKKLGDAADVPVNIGRVTLADFLAYDALIVGSPTLGDGELPGQSVGLNQPSWEEFLPQ
LADADLTGKTVAIFGLGDQKKYAEEFVDAIRLLHDAFADAGARMVGRWPTEGYEFKASQAVDGDEFVGLALDQHHQPVLT
EERIDRWLAQITPELLP

Sequences:

>Translated_177_residues
MAKIGIFFGTDTGRTRLIAKQIAKKLGDAADVPVNIGRVTLADFLAYDALIVGSPTLGDGELPGQSVGLNQPSWEEFLPQ
LADADLTGKTVAIFGLGDQKKYAEEFVDAIRLLHDAFADAGARMVGRWPTEGYEFKASQAVDGDEFVGLALDQHHQPVLT
EERIDRWLAQITPELLP
>Mature_176_residues
AKIGIFFGTDTGRTRLIAKQIAKKLGDAADVPVNIGRVTLADFLAYDALIVGSPTLGDGELPGQSVGLNQPSWEEFLPQL
ADADLTGKTVAIFGLGDQKKYAEEFVDAIRLLHDAFADAGARMVGRWPTEGYEFKASQAVDGDEFVGLALDQHHQPVLTE
ERIDRWLAQITPELLP

Specific function: Low-potential electron donor to a number of redox enzymes. NifF is the electron donor to nitrogenase [H]

COG id: COG0716

COG function: function code C; Flavodoxins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Escherichia coli, GI1786900, Length=177, Percent_Identity=48.5875706214689, Blast_Score=155, Evalue=1e-39,
Organism=Escherichia coli, GI1789262, Length=175, Percent_Identity=41.1428571428571, Blast_Score=127, Evalue=5e-31,

Paralogues:

None

Copy number: 242 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001226
- InterPro:   IPR010086 [H]

Pfam domain/function: PF00258 Flavodoxin_1 [H]

EC number: NA

Molecular weight: Translated: 19221; Mature: 19090

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00201 FLAVODOXIN ; PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIGIFFGTDTGRTRLIAKQIAKKLGDAADVPVNIGRVTLADFLAYDALIVGSPTLGDG
CCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCCCC
ELPGQSVGLNQPSWEEFLPQLADADLTGKTVAIFGLGDQKKYAEEFVDAIRLLHDAFADA
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
GARMVGRWPTEGYEFKASQAVDGDEFVGLALDQHHQPVLTEERIDRWLAQITPELLP
CCHHHCCCCCCCEEECCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKIGIFFGTDTGRTRLIAKQIAKKLGDAADVPVNIGRVTLADFLAYDALIVGSPTLGDG
CEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCCCC
ELPGQSVGLNQPSWEEFLPQLADADLTGKTVAIFGLGDQKKYAEEFVDAIRLLHDAFADA
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
GARMVGRWPTEGYEFKASQAVDGDEFVGLALDQHHQPVLTEERIDRWLAQITPELLP
CCHHHCCCCCCCEEECCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3062178; 3911951; 6337345 [H]