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The map label for this gene is nifB [H]

Identifier: 71907086

GI number: 71907086

Start: 1581298

End: 1582800

Strand: Reverse

Name: nifB [H]

Synonym: Daro_1454

Alternate gene names: 71907086

Gene position: 1582800-1581298 (Counterclockwise)

Preceding gene: 71907088

Following gene: 71907085

Centisome position: 35.16

GC content: 63.07

Gene sequence:

>1503_bases
GTGGAACTTCCAGTCATCAGCAACACCGCGCCGGCCGCAGAAGGCGGCGGCTGTTCGTCCAGCGGTTGCGGCACTGCGCC
GGACGCCCTGGGCCATCTCCCTGACCACATCCGCGCCAAGGTGCAGGATCACCCCTGCTACTCCGAAGAAGCGCACCATT
ACTTCGCCCGCATGCATGTCGCCGTCGCCCCGGCCTGCAACATCCAGTGCAATTACTGCAACCGCAAGTATGACTGCTCC
AACGAGTCCCGCCCGGGCGTCGTTTCCGAGCTGCTGACGCCGGACCAGGCGATCAAGAAGGTCCTCGCCGTCGCCGCCGA
GATTCCTCAGATGACGGTGCTGGGCATCGCCGGACCCGGAGACCCTCTCGCCAACCCGGGCCGCACCTTTGAAACCTTCG
AACAGCTGTCCGCGCGCGCCCCGGACATCAAGCTGTGCGTGTCGACCAACGGCCTGAATCTGCCGCAGTACGTCGACCGC
ATCGCCCAGCACAACATCGACCATGTGACGATCACCATCAACTGCGTCGATCCCGAAGTCGGTGCCAAGATCTATCCGTG
GATCTACTGGGAGAACAAGCGAATCACCGGTGTCGAAGGTGCGCGCATCCTGATCGAACAGCAGCAGAAGGGTCTGCAGA
TGCTGACCGACCGGGGCATCCTGGTGAAGGTCAACTCGGTGCTGATCCCCGGCGTCAATGACGAGCATCTCAAGGAAGTC
TCGAAGATCGTCAAGGCCAAGGGCGCTTTCCTGCACAACGTGATGCCGCTGATCGCCGAGCCCGAGCACGGCACCTACTA
CGGGATCATGGAGCAGCCGGAACCAACGCCGGAAATGCTGCAGGACCTGCAGGATGCCTGTGCCGGCGACATGGCGATGA
TGCGCCACTGCCGCCAGTGCCGCGCCGATGCAGTCGGCCTGTTGGGCGAGGACCGCGGCGACGAGTTCACGCTCGACAAG
ATTGACGTCATGGATGTCGATTACGAAGCCGCCATGGTCCGCCGCAAGGTGGTGCACGACGAGATCATCGAGAAGCTCGA
CGCCAAGCGCGGCATCGTCAAGCCCAAGCACGAAGGCATTCCGGAAGGTTCACGCCCGGTGCTGATGGCGGTGGCTGGCA
AGAACGGTGTCGTCGCCGAGCACTTCGGCCACGCCAAGGAATTCTTGATCTACGAGGCCTCCCCCTCGGGGGTCCGCTTC
ATCAGCCACCGCAAGACCGAGCTGTACTGTGGCGGCATGGATTCCTGCGGCAATGGCGACGTGGTCGATGAAGGCGCAAC
GGAATCCCTGCTCGACCGCAACATCCGCGTGCTCGAAGGCTGCGAGGTCGTGCTCTGCTCCAAGATCGGCTACGAACCCT
GGGGCAAGCTGGAAGCCGCCGGCATCGCGCCGAATGGCGAGCACGCCATGGAGCCGATCGAGGATGCCGTGATGGCGGTG
TACAAGGAAATTGCGGCAACCGGCAAGCTGCTTCCCTCCGCAGAAACCCTGAAGGTGGCGTAA

Upstream 100 bases:

>100_bases
CGCCGAAACTGAATTCATAAGCCGCTGTTTTAAATGGAATTCAATGACTGGCACACCGATTGCTGAATAGGCTCCGCACA
GCATTCGAGGAGCCTTCATC

Downstream 100 bases:

>100_bases
ATGGCACTCCAGATAACCCAATCCTGCGTCAATTGCTGGGCTTGCGTCGATGTCTGCCCGAACGATGCGATCTACCAGGA
CACCCCACACTTCCTGATCG

Product: nitrogenase cofactor biosynthesis protein NifB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MELPVISNTAPAAEGGGCSSSGCGTAPDALGHLPDHIRAKVQDHPCYSEEAHHYFARMHVAVAPACNIQCNYCNRKYDCS
NESRPGVVSELLTPDQAIKKVLAVAAEIPQMTVLGIAGPGDPLANPGRTFETFEQLSARAPDIKLCVSTNGLNLPQYVDR
IAQHNIDHVTITINCVDPEVGAKIYPWIYWENKRITGVEGARILIEQQQKGLQMLTDRGILVKVNSVLIPGVNDEHLKEV
SKIVKAKGAFLHNVMPLIAEPEHGTYYGIMEQPEPTPEMLQDLQDACAGDMAMMRHCRQCRADAVGLLGEDRGDEFTLDK
IDVMDVDYEAAMVRRKVVHDEIIEKLDAKRGIVKPKHEGIPEGSRPVLMAVAGKNGVVAEHFGHAKEFLIYEASPSGVRF
ISHRKTELYCGGMDSCGNGDVVDEGATESLLDRNIRVLEGCEVVLCSKIGYEPWGKLEAAGIAPNGEHAMEPIEDAVMAV
YKEIAATGKLLPSAETLKVA

Sequences:

>Translated_500_residues
MELPVISNTAPAAEGGGCSSSGCGTAPDALGHLPDHIRAKVQDHPCYSEEAHHYFARMHVAVAPACNIQCNYCNRKYDCS
NESRPGVVSELLTPDQAIKKVLAVAAEIPQMTVLGIAGPGDPLANPGRTFETFEQLSARAPDIKLCVSTNGLNLPQYVDR
IAQHNIDHVTITINCVDPEVGAKIYPWIYWENKRITGVEGARILIEQQQKGLQMLTDRGILVKVNSVLIPGVNDEHLKEV
SKIVKAKGAFLHNVMPLIAEPEHGTYYGIMEQPEPTPEMLQDLQDACAGDMAMMRHCRQCRADAVGLLGEDRGDEFTLDK
IDVMDVDYEAAMVRRKVVHDEIIEKLDAKRGIVKPKHEGIPEGSRPVLMAVAGKNGVVAEHFGHAKEFLIYEASPSGVRF
ISHRKTELYCGGMDSCGNGDVVDEGATESLLDRNIRVLEGCEVVLCSKIGYEPWGKLEAAGIAPNGEHAMEPIEDAVMAV
YKEIAATGKLLPSAETLKVA
>Mature_500_residues
MELPVISNTAPAAEGGGCSSSGCGTAPDALGHLPDHIRAKVQDHPCYSEEAHHYFARMHVAVAPACNIQCNYCNRKYDCS
NESRPGVVSELLTPDQAIKKVLAVAAEIPQMTVLGIAGPGDPLANPGRTFETFEQLSARAPDIKLCVSTNGLNLPQYVDR
IAQHNIDHVTITINCVDPEVGAKIYPWIYWENKRITGVEGARILIEQQQKGLQMLTDRGILVKVNSVLIPGVNDEHLKEV
SKIVKAKGAFLHNVMPLIAEPEHGTYYGIMEQPEPTPEMLQDLQDACAGDMAMMRHCRQCRADAVGLLGEDRGDEFTLDK
IDVMDVDYEAAMVRRKVVHDEIIEKLDAKRGIVKPKHEGIPEGSRPVLMAVAGKNGVVAEHFGHAKEFLIYEASPSGVRF
ISHRKTELYCGGMDSCGNGDVVDEGATESLLDRNIRVLEGCEVVLCSKIGYEPWGKLEAAGIAPNGEHAMEPIEDAVMAV
YKEIAATGKLLPSAETLKVA

Specific function: Probably involved in the synthesis of the Fe-Mo cofactor [H]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaA/nifB/pqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003731
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR005980
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02579 Nitro_FeMo-Co; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 54386; Mature: 54386

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELPVISNTAPAAEGGGCSSSGCGTAPDALGHLPDHIRAKVQDHPCYSEEAHHYFARMHV
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHH
AVAPACNIQCNYCNRKYDCSNESRPGVVSELLTPDQAIKKVLAVAAEIPQMTVLGIAGPG
EECCCCCEEECCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCC
DPLANPGRTFETFEQLSARAPDIKLCVSTNGLNLPQYVDRIAQHNIDHVTITINCVDPEV
CCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEEECCCC
GAKIYPWIYWENKRITGVEGARILIEQQQKGLQMLTDRGILVKVNSVLIPGVNDEHLKEV
CCEEEEEEEECCCEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCHHHHHHH
SKIVKAKGAFLHNVMPLIAEPEHGTYYGIMEQPEPTPEMLQDLQDACAGDMAMMRHCRQC
HHHHHHCCHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
RADAVGLLGEDRGDEFTLDKIDVMDVDYEAAMVRRKVVHDEIIEKLDAKRGIVKPKHEGI
HHHHHCCCCCCCCCEEEECEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PEGSRPVLMAVAGKNGVVAEHFGHAKEFLIYEASPSGVRFISHRKTELYCGGMDSCGNGD
CCCCCCEEEEEECCCCEEHHHHCCCEEEEEEECCCCCEEEEECCCCEEEECCCCCCCCCC
VVDEGATESLLDRNIRVLEGCEVVLCSKIGYEPWGKLEAAGIAPNGEHAMEPIEDAVMAV
CCCCCCHHHHHHCCCEEECCCCEEEEHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHH
YKEIAATGKLLPSAETLKVA
HHHHHHHCCCCCCCCCEECC
>Mature Secondary Structure
MELPVISNTAPAAEGGGCSSSGCGTAPDALGHLPDHIRAKVQDHPCYSEEAHHYFARMHV
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHH
AVAPACNIQCNYCNRKYDCSNESRPGVVSELLTPDQAIKKVLAVAAEIPQMTVLGIAGPG
EECCCCCEEECCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCC
DPLANPGRTFETFEQLSARAPDIKLCVSTNGLNLPQYVDRIAQHNIDHVTITINCVDPEV
CCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEEECCCC
GAKIYPWIYWENKRITGVEGARILIEQQQKGLQMLTDRGILVKVNSVLIPGVNDEHLKEV
CCEEEEEEEECCCEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCHHHHHHH
SKIVKAKGAFLHNVMPLIAEPEHGTYYGIMEQPEPTPEMLQDLQDACAGDMAMMRHCRQC
HHHHHHCCHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
RADAVGLLGEDRGDEFTLDKIDVMDVDYEAAMVRRKVVHDEIIEKLDAKRGIVKPKHEGI
HHHHHCCCCCCCCCEEEECEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PEGSRPVLMAVAGKNGVVAEHFGHAKEFLIYEASPSGVRFISHRKTELYCGGMDSCGNGD
CCCCCCEEEEEECCCCEEHHHHCCCEEEEEEECCCCCEEEEECCCCEEEECCCCCCCCCC
VVDEGATESLLDRNIRVLEGCEVVLCSKIGYEPWGKLEAAGIAPNGEHAMEPIEDAVMAV
CCCCCCHHHHHHCCCEEECCCCEEEEHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHH
YKEIAATGKLLPSAETLKVA
HHHHHHHCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2450865 [H]