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The map label for this gene is ureF

Identifier: 71907057

GI number: 71907057

Start: 1552281

End: 1552967

Strand: Reverse

Name: ureF

Synonym: Daro_1425

Alternate gene names: 71907057

Gene position: 1552967-1552281 (Counterclockwise)

Preceding gene: 71907058

Following gene: 71907056

Centisome position: 34.5

GC content: 62.45

Gene sequence:

>687_bases
TTGAAAACCTGCCTCCAACTTGCCCGTCTGCTGCAACTGGCCAGCCCGACGCTGCCGGTTGGCGCCTACACCTATTCGCA
GGGACTGGAATGGGCGGTTGAATCGGGTGTTATCCGCGACGAGGCGAGCGCAGGCCGCTGGATTGCCGATCTGATGCACC
ACGGCATCGGCTGTTACGAAGCGCCTATGGTTGCCGCGCTAATGACGGCATGGTCGGCCGGCGATGTCGACGAGATTGAA
TGCCTCAATGCCGAATTCCTGGCCAGCCGGGAATCGGCCGAACTGCGCGCCGAAACCGTCCAGATGGGCTTTTCCATGCG
CCGCTTGCTGCGCGATTTGCGCGATGACACGCTGGCCGCAGTCGCCACACTGGTCGAAACCCAAGCAGAAGTGGCCTTTC
CCACCGTCTGGTCGGGGATCGCAGCCGCCTGGCAGATCGAACCGGAAGCCGCAGTCACCGCCTACCTGTGGTCATGGGCC
GAAAATCAGGTGATGGCCGCACTGAAAGCTGTACCACTTGGGCAGGCCTCCGGGCAACGCCTGCTGGCCGAACTTGGCAG
CCGGATTCCTGAGGCGGCAGCAAACGCCAACACCCTGCCGAAATCCCGGTGGTCCAACTTCACGCCGGCCTTCGCCATCG
CCTGCGCCCGCCACGAAACCCAATATTCACGTCTCTTCAGATCGTAA

Upstream 100 bases:

>100_bases
GTGGCGGCCACCACCACGGCGACGAATCCGCTACCGACCTGCACAACCCCGGCCACGGCCCACATCGGTCCGTACCGAAA
ATTCACGAGTTCAAGCCGCG

Downstream 100 bases:

>100_bases
AATCATGAGCAAACAAGCACTCCGCGTCGGTATCGGCGGCCCGGTCGGGTCCGGCAAGACTGCCCTCACCCTCGCCCTCT
GCCAAGCCCTGCGCGAGCAA

Product: urease accessory protein UreF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MKTCLQLARLLQLASPTLPVGAYTYSQGLEWAVESGVIRDEASAGRWIADLMHHGIGCYEAPMVAALMTAWSAGDVDEIE
CLNAEFLASRESAELRAETVQMGFSMRRLLRDLRDDTLAAVATLVETQAEVAFPTVWSGIAAAWQIEPEAAVTAYLWSWA
ENQVMAALKAVPLGQASGQRLLAELGSRIPEAAANANTLPKSRWSNFTPAFAIACARHETQYSRLFRS

Sequences:

>Translated_228_residues
MKTCLQLARLLQLASPTLPVGAYTYSQGLEWAVESGVIRDEASAGRWIADLMHHGIGCYEAPMVAALMTAWSAGDVDEIE
CLNAEFLASRESAELRAETVQMGFSMRRLLRDLRDDTLAAVATLVETQAEVAFPTVWSGIAAAWQIEPEAAVTAYLWSWA
ENQVMAALKAVPLGQASGQRLLAELGSRIPEAAANANTLPKSRWSNFTPAFAIACARHETQYSRLFRS
>Mature_228_residues
MKTCLQLARLLQLASPTLPVGAYTYSQGLEWAVESGVIRDEASAGRWIADLMHHGIGCYEAPMVAALMTAWSAGDVDEIE
CLNAEFLASRESAELRAETVQMGFSMRRLLRDLRDDTLAAVATLVETQAEVAFPTVWSGIAAAWQIEPEAAVTAYLWSWA
ENQVMAALKAVPLGQASGQRLLAELGSRIPEAAANANTLPKSRWSNFTPAFAIACARHETQYSRLFRS

Specific function: Required for maturation of urease via the functional incorporation of the urease nickel metallocenter

COG id: COG0830

COG function: function code O; Urease accessory protein UreF

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ureF family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UREF_DECAR (Q47G57)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_284644.1
- STRING:   Q47G57
- GeneID:   3569071
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_1425
- NMPDR:   fig|159087.4.peg.1278
- eggNOG:   COG0830
- HOGENOM:   HBG709295
- OMA:   SPQLPIG
- ProtClustDB:   CLSK768358
- BioCyc:   DARO159087:DARO_1425-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01385
- InterPro:   IPR002639
- PIRSF:   PIRSF009467

Pfam domain/function: PF01730 UreF

EC number: NA

Molecular weight: Translated: 24862; Mature: 24862

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTCLQLARLLQLASPTLPVGAYTYSQGLEWAVESGVIRDEASAGRWIADLMHHGIGCYE
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHCCCCHHH
APMVAALMTAWSAGDVDEIECLNAEFLASRESAELRAETVQMGFSMRRLLRDLRDDTLAA
HHHHHHHHHHHCCCCCHHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VATLVETQAEVAFPTVWSGIAAAWQIEPEAAVTAYLWSWAENQVMAALKAVPLGQASGQR
HHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LLAELGSRIPEAAANANTLPKSRWSNFTPAFAIACARHETQYSRLFRS
HHHHHHHHCCHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTCLQLARLLQLASPTLPVGAYTYSQGLEWAVESGVIRDEASAGRWIADLMHHGIGCYE
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHCCCCHHH
APMVAALMTAWSAGDVDEIECLNAEFLASRESAELRAETVQMGFSMRRLLRDLRDDTLAA
HHHHHHHHHHHCCCCCHHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VATLVETQAEVAFPTVWSGIAAAWQIEPEAAVTAYLWSWAENQVMAALKAVPLGQASGQR
HHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LLAELGSRIPEAAANANTLPKSRWSNFTPAFAIACARHETQYSRLFRS
HHHHHHHHCCHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA