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The map label for this gene is azoR

Identifier: 71907023

GI number: 71907023

Start: 1521143

End: 1521742

Strand: Reverse

Name: azoR

Synonym: Daro_1391

Alternate gene names: 71907023

Gene position: 1521742-1521143 (Counterclockwise)

Preceding gene: 71907025

Following gene: 71907022

Centisome position: 33.81

GC content: 63.0

Gene sequence:

>600_bases
ATGAAAATCCTGCAAATCAATGCCAGCGCCCGTTCTGCCGGTGCCAACTCGACCCGCCTGGCCGACACCGTCACGGCTCG
CCTGCTGGCGCGAAATCCGAATGCCGTGGTCGAACTGCGCGATCTGGCCAGTCACCCGCATCCGGTGCTCGACGAACCGG
CGCTCGGCGCCTTGTTCACGCCGGCCGATCAGCGTACAGCCGAACAGTCGGCCCGCGTGGCCCTCGATGATGCGCTGATT
GCCCAGGTTCAGTCGGTCGATGCCATCGTGCTCGGCGTCCCGATGTACAACTTCGGCGTGCCGGTCCAGCTGAAAACCTG
GATCGACGCCATCGCCCGGGCCGGCGTCACCTTCCACTACACCGAGAAGGGTCCGGAAGGACTGCTCAAGGGCAAGAAGG
TTTATGTCGCCCTGGCCCGCGGCGGTCTTTATCGCGATACCCCGGCCGACTCCCAGGTGCCTTACCTGAAGAGCGTGCTC
GGCTTTCTCGGCATGACCGACGTCGAGTTCATCTACGCCGAAGGCCTGGCGATGGGTGCCGAGTCAGCCAGCAAGGCGTT
TGCCGAAGCCGAAGCACAGATTGAAGAATTGATTGCCTAA

Upstream 100 bases:

>100_bases
TTTCAGCGGCTTCCGGATCGATATTGTGACCAATAGAATTCACTTCATGGTTTCAGCGGTTTGAACCACTTCCCACCACC
CCACATTGGAGAAAACGATC

Downstream 100 bases:

>100_bases
CAACGAAGCGCGGAGGCGTTCAGAAATGAGCGCTTCCACCCTGCCCCCATTGGAGAAAATCATGAACACAGCCATATCCA
GCCATCAATTCAGAACGGTT

Product: NAD(P)H dehydrogenase (quinone):NADPH-dependent FMN reductase

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase; FMN-dependent NADH-azo compound oxidoreductase

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MKILQINASARSAGANSTRLADTVTARLLARNPNAVVELRDLASHPHPVLDEPALGALFTPADQRTAEQSARVALDDALI
AQVQSVDAIVLGVPMYNFGVPVQLKTWIDAIARAGVTFHYTEKGPEGLLKGKKVYVALARGGLYRDTPADSQVPYLKSVL
GFLGMTDVEFIYAEGLAMGAESASKAFAEAEAQIEELIA

Sequences:

>Translated_199_residues
MKILQINASARSAGANSTRLADTVTARLLARNPNAVVELRDLASHPHPVLDEPALGALFTPADQRTAEQSARVALDDALI
AQVQSVDAIVLGVPMYNFGVPVQLKTWIDAIARAGVTFHYTEKGPEGLLKGKKVYVALARGGLYRDTPADSQVPYLKSVL
GFLGMTDVEFIYAEGLAMGAESASKAFAEAEAQIEELIA
>Mature_199_residues
MKILQINASARSAGANSTRLADTVTARLLARNPNAVVELRDLASHPHPVLDEPALGALFTPADQRTAEQSARVALDDALI
AQVQSVDAIVLGVPMYNFGVPVQLKTWIDAIARAGVTFHYTEKGPEGLLKGKKVYVALARGGLYRDTPADSQVPYLKSVL
GFLGMTDVEFIYAEGLAMGAESASKAFAEAEAQIEELIA

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family

Homologues:

Organism=Escherichia coli, GI1787680, Length=199, Percent_Identity=44.2211055276382, Blast_Score=174, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AZOR_DECAR (Q47G91)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_284610.1
- ProteinModelPortal:   Q47G91
- SMR:   Q47G91
- STRING:   Q47G91
- GeneID:   3566119
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_1391
- NMPDR:   fig|159087.4.peg.1211
- eggNOG:   COG1182
- HOGENOM:   HBG565834
- OMA:   YFDFIAR
- ProtClustDB:   CLSK713301
- BioCyc:   DARO159087:DARO_1391-MONOMER
- HAMAP:   MF_01216
- InterPro:   IPR003680
- InterPro:   IPR023048

Pfam domain/function: PF02525 Flavodoxin_2

EC number: 3.1.4.14

Molecular weight: Translated: 21191; Mature: 21191

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILQINASARSAGANSTRLADTVTARLLARNPNAVVELRDLASHPHPVLDEPALGALFT
CEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHHCCCCCCCCCCCCCCEEC
PADQRTAEQSARVALDDALIAQVQSVDAIVLGVPMYNFGVPVQLKTWIDAIARAGVTFHY
CCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHCCCCEEHHHHHHHHHHCCCEEEE
TEKGPEGLLKGKKVYVALARGGLYRDTPADSQVPYLKSVLGFLGMTDVEFIYAEGLAMGA
CCCCCHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCHHCCC
ESASKAFAEAEAQIEELIA
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILQINASARSAGANSTRLADTVTARLLARNPNAVVELRDLASHPHPVLDEPALGALFT
CEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHHCCCCCCCCCCCCCCEEC
PADQRTAEQSARVALDDALIAQVQSVDAIVLGVPMYNFGVPVQLKTWIDAIARAGVTFHY
CCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHCCCCEEHHHHHHHHHHCCCEEEE
TEKGPEGLLKGKKVYVALARGGLYRDTPADSQVPYLKSVLGFLGMTDVEFIYAEGLAMGA
CCCCCHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCHHCCC
ESASKAFAEAEAQIEELIA
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA