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The map label for this gene is dmpD [H]

Identifier: 71906963

GI number: 71906963

Start: 1448769

End: 1449605

Strand: Reverse

Name: dmpD [H]

Synonym: Daro_1331

Alternate gene names: 71906963

Gene position: 1449605-1448769 (Counterclockwise)

Preceding gene: 71906965

Following gene: 71906961

Centisome position: 32.21

GC content: 48.98

Gene sequence:

>837_bases
ATGAGTAAAACGAGCCCAGAAATTGCCAACAGCACGATCGCGAACGGTATCCGCACAAACTACCATGACACAGGCAGCGG
ATTTCCGCTACTGATGATCCACGGATCCGGTCCTGGAGTATCTGCATGGGCGAATTGGCGATTGGTAATACCCGAACTTG
CAAAGCAGTCACGTGTTATCGCCCCCGACATGGTCGGATTTGGCTTTACTGAACGTCCTGATGGGCAAGAGTACAACATG
GACGTATGGGTAAATCAGGCGATTGGTCTGCTTGATTCGCTCCAAATCGAACAAACTGATCTCGTGGGAAATTCCTTTGG
TGGCGCTTTAGCCTTAGCGCTGAGCATCCGCTACCCAAAGCGCGTTCGCCGACTCGTGCTCATGGGAAGCGCCGGAGTTC
CATTTGATATTACACCTGGACTTGATGCAGTTTGGGGCTATACACCTTCAATTGCAAACATGCGGGCTTTGTTAGATATC
TTTGCATGGGATCGCAACCTCGTTAATGACGAACTAGCAAAACTTCGTTATGAGGCGAGTATCCAACCAGGATTCCAGGA
GTCTTTCTCGAAAATGTTCCCCGCCCCGCGTCAGCGATGGGTCGATGCCTTGGCAAGTTCAGAAGAAAACATACGCTCGC
TGCCCCATCACACTTTGATTATTCATGGCCGCGAAGATGCAGTAATCCCTTTATCAAATTCAATTAGGCTAGCCGATCTC
ATTCCAAACTCTCAATTACACGTTTACGGGCATTGTGGTCATTGGACGCAAATTGAGCATGCAGCACGCTTTGCAAAATT
AGTCTCGGATTTCATTGGTGAGTGCAACGATGTTTAG

Upstream 100 bases:

>100_bases
AATCACAGGCCATTAGTTTTCAAGTATAGCCAAAACCAGATTTTACCCGAACCAAAATCATCTATTTTTACCCCACAAGA
TTGCAAAAGGAGAGTTACTT

Downstream 100 bases:

>100_bases
ACATGTCTTTAGCCGAGCACACAATGTCAGATGGAAGTCCCCCTGTAGCCACCGGCGACCGATCCTGACCACCTAATAAA
GCATTTTTCCTACCTAAATA

Product: Alpha/beta hydrolase fold

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: HMSH [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MSKTSPEIANSTIANGIRTNYHDTGSGFPLLMIHGSGPGVSAWANWRLVIPELAKQSRVIAPDMVGFGFTERPDGQEYNM
DVWVNQAIGLLDSLQIEQTDLVGNSFGGALALALSIRYPKRVRRLVLMGSAGVPFDITPGLDAVWGYTPSIANMRALLDI
FAWDRNLVNDELAKLRYEASIQPGFQESFSKMFPAPRQRWVDALASSEENIRSLPHHTLIIHGREDAVIPLSNSIRLADL
IPNSQLHVYGHCGHWTQIEHAARFAKLVSDFIGECNDV

Sequences:

>Translated_278_residues
MSKTSPEIANSTIANGIRTNYHDTGSGFPLLMIHGSGPGVSAWANWRLVIPELAKQSRVIAPDMVGFGFTERPDGQEYNM
DVWVNQAIGLLDSLQIEQTDLVGNSFGGALALALSIRYPKRVRRLVLMGSAGVPFDITPGLDAVWGYTPSIANMRALLDI
FAWDRNLVNDELAKLRYEASIQPGFQESFSKMFPAPRQRWVDALASSEENIRSLPHHTLIIHGREDAVIPLSNSIRLADL
IPNSQLHVYGHCGHWTQIEHAARFAKLVSDFIGECNDV
>Mature_277_residues
SKTSPEIANSTIANGIRTNYHDTGSGFPLLMIHGSGPGVSAWANWRLVIPELAKQSRVIAPDMVGFGFTERPDGQEYNMD
VWVNQAIGLLDSLQIEQTDLVGNSFGGALALALSIRYPKRVRRLVLMGSAGVPFDITPGLDAVWGYTPSIANMRALLDIF
AWDRNLVNDELAKLRYEASIQPGFQESFSKMFPAPRQRWVDALASSEENIRSLPHHTLIIHGREDAVIPLSNSIRLADLI
PNSQLHVYGHCGHWTQIEHAARFAKLVSDFIGECNDV

Specific function: Conversion of 2-hydroxymuconic acid semialdehyde to 2- hydroxypent-2,4-dienoate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI27597073, Length=299, Percent_Identity=23.7458193979933, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI218777837, Length=116, Percent_Identity=31.8965517241379, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI87081721, Length=267, Percent_Identity=31.0861423220974, Blast_Score=128, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17558492, Length=123, Percent_Identity=34.9593495934959, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI32566936, Length=123, Percent_Identity=34.9593495934959, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25146278, Length=137, Percent_Identity=31.3868613138686, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324392, Length=128, Percent_Identity=30.46875, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 30731; Mature: 30600

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTSPEIANSTIANGIRTNYHDTGSGFPLLMIHGSGPGVSAWANWRLVIPELAKQSRVI
CCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEECHHHHCCCCCC
APDMVGFGFTERPDGQEYNMDVWVNQAIGLLDSLQIEQTDLVGNSFGGALALALSIRYPK
CCHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCHHHHCCCCCCCCEEEEEECCCHH
RVRRLVLMGSAGVPFDITPGLDAVWGYTPSIANMRALLDIFAWDRNLVNDELAKLRYEAS
HHHHEEEECCCCCCEECCCCCHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
IQPGFQESFSKMFPAPRQRWVDALASSEENIRSLPHHTLIIHGREDAVIPLSNSIRLADL
CCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHCCCEEEEEECCCCEEEECCCCEEEEEE
IPNSQLHVYGHCGHWTQIEHAARFAKLVSDFIGECNDV
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKTSPEIANSTIANGIRTNYHDTGSGFPLLMIHGSGPGVSAWANWRLVIPELAKQSRVI
CCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEECHHHHCCCCCC
APDMVGFGFTERPDGQEYNMDVWVNQAIGLLDSLQIEQTDLVGNSFGGALALALSIRYPK
CCHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCHHHHCCCCCCCCEEEEEECCCHH
RVRRLVLMGSAGVPFDITPGLDAVWGYTPSIANMRALLDIFAWDRNLVNDELAKLRYEAS
HHHHEEEECCCCCCEECCCCCHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
IQPGFQESFSKMFPAPRQRWVDALASSEENIRSLPHHTLIIHGREDAVIPLSNSIRLADL
CCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHCCCEEEEEECCCCEEEECCCCEEEEEE
IPNSQLHVYGHCGHWTQIEHAARFAKLVSDFIGECNDV
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2194577 [H]