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The map label for this gene is dmlR [H]

Identifier: 71906775

GI number: 71906775

Start: 1242138

End: 1243058

Strand: Reverse

Name: dmlR [H]

Synonym: Daro_1136

Alternate gene names: 71906775

Gene position: 1243058-1242138 (Counterclockwise)

Preceding gene: 71906789

Following gene: 71906773

Centisome position: 27.62

GC content: 64.71

Gene sequence:

>921_bases
ATGAGTTCCGAACTGGCTTTCTTCAGTCTGCTCGTTCAGCAAGGCAGTCTGGTCCAGGCCGCCCGCGAGCTTGGCCTGAC
CGGCCCGGCGGTCAGCCGTCGCCTCAGCCAGCTTGAGCAACGACTTGGCGTCCGCCTGCTGGCCCGCACCACACGGCGGA
TGAGCCTGACGCCGGAAGGCGAACTTTATCTGGCCGAAAGCCGCCGCATCCTCGGCGAAATCGAGGCACTGGAGCAGGCC
CTTAACCGGACCCGGGCGGAACCGCGCGGCCTGCTGCGCATCCACGCCACCTTCGGCTTCGGTCGCCGGCAACTGGCACC
GGCTGTCTCGGAATTTGTCCGCCAGCATCCTGCCGTCGATATCCAGCTCACTTTGAGCGACCAGCCAGTCACGCCCGGTG
AGCAGGGATTCGACATCGCCATCCGCTTCGGCGAGCCACCGGAGGCTCGCGTCGTTGCCCGCCAGATTGCCTCCAACCAG
CGTTACCTGTTTGCCTCGCCGGCTTACCTGGCCAGACGCGGCCAGCCGCTGACGCTGGATGAACTCGTCAACCACGACTG
CATCGTCATCCGCGAAGGTACCGGTGCCTTTGGCACGTGGACGCTGTGTTCGGGCAAGCAATGCCGGAACATCAAGGTCA
GCGGCAAGTTGAGCAGCAATCACGGTGAAGTCGCCGTCGATTGGGCACTCGACGGCCATGGCATCCTGCTCCGCTCATTG
TGGGACACCGCCACCGACCTGCGGGCTGGTCGCCTGGTTCGTGTGCTGCCCGAATGGTCGGGCAGCCCGGCCGACATCTA
TGCGCTTTACCCGCAGCGCCTGAACCTGTCGGCCAAGGTGCGGGTATTTCTCGATTTTCTGACTGAGCGCTTCGCCGCCT
ACCGCAGCGCCACCGACAGCGAGGCCGAATTGCCCTGGTAA

Upstream 100 bases:

>100_bases
ACCGGACTCGAAAAAGTATCGGCTCATGATACTGCTTTGCTGAAAGGCAAAAATATCGCTAAATTAACTCCATTCGTTAA
TTTAAATCATCAATAAAACC

Downstream 100 bases:

>100_bases
CCCGATGTCAGCGGGAAGCCCGCCACAGGGGATAGAGGCCGAGCAGACCAAGCAGCGGCCCGGGCAAGAGGAGCCACGCG
ATACTCGTCCCCCAACCGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSSELAFFSLLVQQGSLVQAARELGLTGPAVSRRLSQLEQRLGVRLLARTTRRMSLTPEGELYLAESRRILGEIEALEQA
LNRTRAEPRGLLRIHATFGFGRRQLAPAVSEFVRQHPAVDIQLTLSDQPVTPGEQGFDIAIRFGEPPEARVVARQIASNQ
RYLFASPAYLARRGQPLTLDELVNHDCIVIREGTGAFGTWTLCSGKQCRNIKVSGKLSSNHGEVAVDWALDGHGILLRSL
WDTATDLRAGRLVRVLPEWSGSPADIYALYPQRLNLSAKVRVFLDFLTERFAAYRSATDSEAELPW

Sequences:

>Translated_306_residues
MSSELAFFSLLVQQGSLVQAARELGLTGPAVSRRLSQLEQRLGVRLLARTTRRMSLTPEGELYLAESRRILGEIEALEQA
LNRTRAEPRGLLRIHATFGFGRRQLAPAVSEFVRQHPAVDIQLTLSDQPVTPGEQGFDIAIRFGEPPEARVVARQIASNQ
RYLFASPAYLARRGQPLTLDELVNHDCIVIREGTGAFGTWTLCSGKQCRNIKVSGKLSSNHGEVAVDWALDGHGILLRSL
WDTATDLRAGRLVRVLPEWSGSPADIYALYPQRLNLSAKVRVFLDFLTERFAAYRSATDSEAELPW
>Mature_305_residues
SSELAFFSLLVQQGSLVQAARELGLTGPAVSRRLSQLEQRLGVRLLARTTRRMSLTPEGELYLAESRRILGEIEALEQAL
NRTRAEPRGLLRIHATFGFGRRQLAPAVSEFVRQHPAVDIQLTLSDQPVTPGEQGFDIAIRFGEPPEARVVARQIASNQR
YLFASPAYLARRGQPLTLDELVNHDCIVIREGTGAFGTWTLCSGKQCRNIKVSGKLSSNHGEVAVDWALDGHGILLRSLW
DTATDLRAGRLVRVLPEWSGSPADIYALYPQRLNLSAKVRVFLDFLTERFAAYRSATDSEAELPW

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=292, Percent_Identity=40.4109589041096, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI1789440, Length=287, Percent_Identity=37.2822299651568, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1786401, Length=291, Percent_Identity=36.7697594501718, Blast_Score=164, Evalue=9e-42,
Organism=Escherichia coli, GI145693193, Length=291, Percent_Identity=32.9896907216495, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1787128, Length=286, Percent_Identity=31.4685314685315, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787589, Length=293, Percent_Identity=27.6450511945392, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1789639, Length=288, Percent_Identity=26.7361111111111, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786448, Length=276, Percent_Identity=27.8985507246377, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788706, Length=245, Percent_Identity=25.3061224489796, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1790011, Length=292, Percent_Identity=24.3150684931507, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788481, Length=287, Percent_Identity=25.0871080139373, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI157672245, Length=123, Percent_Identity=32.520325203252, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1789173, Length=249, Percent_Identity=24.8995983935743, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787601, Length=169, Percent_Identity=25.4437869822485, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34017; Mature: 33886

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSELAFFSLLVQQGSLVQAARELGLTGPAVSRRLSQLEQRLGVRLLARTTRRMSLTPEG
CCCHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
ELYLAESRRILGEIEALEQALNRTRAEPRGLLRIHATFGFGRRQLAPAVSEFVRQHPAVD
CEEEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEE
IQLTLSDQPVTPGEQGFDIAIRFGEPPEARVVARQIASNQRYLFASPAYLARRGQPLTLD
EEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEECCHHHHHCCCCCCHH
ELVNHDCIVIREGTGAFGTWTLCSGKQCRNIKVSGKLSSNHGEVAVDWALDGHGILLRSL
HHCCCCEEEEECCCCCCEEEEEECCCCCCEEEEEEEECCCCCCEEEEEEECCCCHHHHHH
WDTATDLRAGRLVRVLPEWSGSPADIYALYPQRLNLSAKVRVFLDFLTERFAAYRSATDS
HHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
EAELPW
CCCCCC
>Mature Secondary Structure 
SSELAFFSLLVQQGSLVQAARELGLTGPAVSRRLSQLEQRLGVRLLARTTRRMSLTPEG
CCHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
ELYLAESRRILGEIEALEQALNRTRAEPRGLLRIHATFGFGRRQLAPAVSEFVRQHPAVD
CEEEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEE
IQLTLSDQPVTPGEQGFDIAIRFGEPPEARVVARQIASNQRYLFASPAYLARRGQPLTLD
EEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEECCHHHHHCCCCCCHH
ELVNHDCIVIREGTGAFGTWTLCSGKQCRNIKVSGKLSSNHGEVAVDWALDGHGILLRSL
HHCCCCEEEEECCCCCCEEEEEECCCCCCEEEEEEEECCCCCCEEEEEEECCCCHHHHHH
WDTATDLRAGRLVRVLPEWSGSPADIYALYPQRLNLSAKVRVFLDFLTERFAAYRSATDS
HHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
EAELPW
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]