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The map label for this gene is grpE

Identifier: 71906563

GI number: 71906563

Start: 1002174

End: 1002728

Strand: Reverse

Name: grpE

Synonym: Daro_0924

Alternate gene names: 71906563

Gene position: 1002728-1002174 (Counterclockwise)

Preceding gene: 71906565

Following gene: 71906562

Centisome position: 22.28

GC content: 59.46

Gene sequence:

>555_bases
ATGTCTAACCCCGAAAATGCCCAGGACGCAATGCTGGGCAACGAACCCGCCGTTGAACCGGTCGTCGAACCGACCGCCGA
GCAACACACCGATACCCTGCCGAGCATTGAAGAGCAATTTCGCGCCCTCGAACTGAAGGCGGCCGAACACTACGATGCCT
GGCTGCGCGCCAAGGCCGAAGGCGAAAATATCCGCCGCCGTGCCCAGGACGACATTTCCAAGGCTCACAAATTCGCTGTC
GAGAAATTCGCTGGCGAACTGCTGGCCGTCAAGGACAGCCTGGAAGCCGCACTGGCCGTGCAGGAACAGACCGTCGACAG
CTTCAAGTCCGGCGTCGAGCTGACCCTGAAGCAACTGGTCTCTGCCTTCGACAAGAACGCGCTGAACGAAGTCAATCCGG
CCGGTGAAAAATTCGACCCGCACAAGCATCAGGCGATCGGCATGGTGGATTCCGAACAGGAAGCCAACACCGTGGTCACC
GTGCTGCAAAAGGGCTACATGATTGCCGACCGCGTGTTGCGTCCGGCCCTCGTCATGGTCGCCAAGGGCAAATAA

Upstream 100 bases:

>100_bases
GCGCTGTAGAGGATTTCAACGATAAAGATTTTCTCCAAGTCCTCTTGAAAGAGCATTTTCCGACCCCATCTACCCAAACA
GCTTTTTTGGAGAAATCTGC

Downstream 100 bases:

>100_bases
ATACTTGAAATCGGGGGCCGCGCCCCCAGTTCACGAACATCAGCAATTCATCTTAAGGAATCGAACAATGGGCAAGATCA
TCGGTATTGACCTCGGCACC

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MSNPENAQDAMLGNEPAVEPVVEPTAEQHTDTLPSIEEQFRALELKAAEHYDAWLRAKAEGENIRRRAQDDISKAHKFAV
EKFAGELLAVKDSLEAALAVQEQTVDSFKSGVELTLKQLVSAFDKNALNEVNPAGEKFDPHKHQAIGMVDSEQEANTVVT
VLQKGYMIADRVLRPALVMVAKGK

Sequences:

>Translated_184_residues
MSNPENAQDAMLGNEPAVEPVVEPTAEQHTDTLPSIEEQFRALELKAAEHYDAWLRAKAEGENIRRRAQDDISKAHKFAV
EKFAGELLAVKDSLEAALAVQEQTVDSFKSGVELTLKQLVSAFDKNALNEVNPAGEKFDPHKHQAIGMVDSEQEANTVVT
VLQKGYMIADRVLRPALVMVAKGK
>Mature_183_residues
SNPENAQDAMLGNEPAVEPVVEPTAEQHTDTLPSIEEQFRALELKAAEHYDAWLRAKAEGENIRRRAQDDISKAHKFAVE
KFAGELLAVKDSLEAALAVQEQTVDSFKSGVELTLKQLVSAFDKNALNEVNPAGEKFDPHKHQAIGMVDSEQEANTVVTV
LQKGYMIADRVLRPALVMVAKGK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=156, Percent_Identity=35.2564102564103, Blast_Score=83, Evalue=1e-16,
Organism=Homo sapiens, GI40255109, Length=137, Percent_Identity=35.036496350365, Blast_Score=75, Evalue=3e-14,
Organism=Escherichia coli, GI1788967, Length=176, Percent_Identity=42.6136363636364, Blast_Score=111, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17552458, Length=154, Percent_Identity=30.5194805194805, Blast_Score=73, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6324806, Length=156, Percent_Identity=35.2564102564103, Blast_Score=86, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24653432, Length=171, Percent_Identity=33.3333333333333, Blast_Score=88, Evalue=3e-18,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_DECAR (Q47HK1)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_284150.1
- ProteinModelPortal:   Q47HK1
- SMR:   Q47HK1
- STRING:   Q47HK1
- GeneID:   3570081
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_0924
- NMPDR:   fig|159087.4.peg.1311
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   LEHNVEG
- BioCyc:   DARO159087:DARO_0924-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 20233; Mature: 20102

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNPENAQDAMLGNEPAVEPVVEPTAEQHTDTLPSIEEQFRALELKAAEHYDAWLRAKAE
CCCCCCCHHHHCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
GENIRRRAQDDISKAHKFAVEKFAGELLAVKDSLEAALAVQEQTVDSFKSGVELTLKQLV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAFDKNALNEVNPAGEKFDPHKHQAIGMVDSEQEANTVVTVLQKGYMIADRVLRPALVMV
HHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHE
AKGK
ECCC
>Mature Secondary Structure 
SNPENAQDAMLGNEPAVEPVVEPTAEQHTDTLPSIEEQFRALELKAAEHYDAWLRAKAE
CCCCCCHHHHCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
GENIRRRAQDDISKAHKFAVEKFAGELLAVKDSLEAALAVQEQTVDSFKSGVELTLKQLV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAFDKNALNEVNPAGEKFDPHKHQAIGMVDSEQEANTVVTVLQKGYMIADRVLRPALVMV
HHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHE
AKGK
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA