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The map label for this gene is livM [H]

Identifier: 71906008

GI number: 71906008

Start: 398070

End: 399002

Strand: Reverse

Name: livM [H]

Synonym: Daro_0366

Alternate gene names: 71906008

Gene position: 399002-398070 (Counterclockwise)

Preceding gene: 71906009

Following gene: 71906007

Centisome position: 8.86

GC content: 64.52

Gene sequence:

>933_bases
ATGAAAAAAAGTGTCTATAACGGCCTCTATGTGGTCATCGCCATCCTGCTGGTGCTGCCTTTCGTGATGCCCAACAGTTT
CTATCTCGATCTCGCGGTGCGCATGGCGATCAATGCCGTGATCGTTCTCGGGCTGAACCTGCTGATCGGCTTTGCCGGCC
AGATCAGCCTTGGCCACGCCGGCTTCCTCGGCATTGGCGCCTACGCCTCGGCCGTGCTCCCGACGCACTTCGGCTGGCAT
CCGCTGCTCGCCATGGGCGCCGGCGCCGTGGCTACCGGCATTCTCGCCGCCGTGGTCGCCCGCCCGATCTTCAAGCTCAA
GGGCAACTACTTGGCGATGGCCACGCTCGGCCTCGGCATCATCATCAGCATTGCGCTGCGCAACGAGGCGCAATGGACCG
GTGGGCCGGACGGCATGCCGGTGCCGGCGATGGGCCTGTTCGGCTTCGAGCTGAGCAACGACAAGCAGTGGTACTGGGTC
GTCGCCCTGCTGCTGTCGGTGTCGGTCTGGGCCTCGCTGAACCTGATCGACTCGCCGTTCGGCCGCGCCCTGCGCGCCCT
GCACGGTTCGGAAGTCGCTTCGCAGGTGGTCGGCGTCGATGTCGTCCGCTACAAGGTCGCCATCTTCGTGCTGTCCGCCG
TCTTCGCCAGCCTGATGGGCAGCGTCACGGCCCACTACGTCGGTTTCGTCAGCCCCAACTTCGCCGACTTCTTCCACTCC
ATCGAACTGGTCACCATGGTCGTCATCGGCGGCATGGCCTCGGTCTATGGCTCGCTGGTCGGCGCCGTGCTGCTCACCGC
CCTGCCGCAAGCCCTGGCCACCTTCGAAGGCTGGGAAACCGTGGTCTTCGGCGCCATCCTGATGCTGTCGATGATCTTCC
TGCCCAAGGGCCTCGTCCCGACGCTGGCCGCCAAATTCGGCAAGGGGGAATAA

Upstream 100 bases:

>100_bases
CGGCCTACAAGGATGCGATCCCCTTCGTGTTGATCCTGTTCATTCTCTTCTTCATGCCGCGCGGCCTGTTCGGCGGCAAA
TCGACGGATCGGGTGTAAGC

Downstream 100 bases:

>100_bases
ATCATGAGCATGCTCGAAGTCAAAGACCTCTCCATCCACTTCGGCGGCGTCAAGGCCGTCCAGAACGTGTCGTTCAACAT
CGATTCCGGCATCGTCTATT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MKKSVYNGLYVVIAILLVLPFVMPNSFYLDLAVRMAINAVIVLGLNLLIGFAGQISLGHAGFLGIGAYASAVLPTHFGWH
PLLAMGAGAVATGILAAVVARPIFKLKGNYLAMATLGLGIIISIALRNEAQWTGGPDGMPVPAMGLFGFELSNDKQWYWV
VALLLSVSVWASLNLIDSPFGRALRALHGSEVASQVVGVDVVRYKVAIFVLSAVFASLMGSVTAHYVGFVSPNFADFFHS
IELVTMVVIGGMASVYGSLVGAVLLTALPQALATFEGWETVVFGAILMLSMIFLPKGLVPTLAAKFGKGE

Sequences:

>Translated_310_residues
MKKSVYNGLYVVIAILLVLPFVMPNSFYLDLAVRMAINAVIVLGLNLLIGFAGQISLGHAGFLGIGAYASAVLPTHFGWH
PLLAMGAGAVATGILAAVVARPIFKLKGNYLAMATLGLGIIISIALRNEAQWTGGPDGMPVPAMGLFGFELSNDKQWYWV
VALLLSVSVWASLNLIDSPFGRALRALHGSEVASQVVGVDVVRYKVAIFVLSAVFASLMGSVTAHYVGFVSPNFADFFHS
IELVTMVVIGGMASVYGSLVGAVLLTALPQALATFEGWETVVFGAILMLSMIFLPKGLVPTLAAKFGKGE
>Mature_310_residues
MKKSVYNGLYVVIAILLVLPFVMPNSFYLDLAVRMAINAVIVLGLNLLIGFAGQISLGHAGFLGIGAYASAVLPTHFGWH
PLLAMGAGAVATGILAAVVARPIFKLKGNYLAMATLGLGIIISIALRNEAQWTGGPDGMPVPAMGLFGFELSNDKQWYWV
VALLLSVSVWASLNLIDSPFGRALRALHGSEVASQVVGVDVVRYKVAIFVLSAVFASLMGSVTAHYVGFVSPNFADFFHS
IELVTMVVIGGMASVYGSLVGAVLLTALPQALATFEGWETVVFGAILMLSMIFLPKGLVPTLAAKFGKGE

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=308, Percent_Identity=34.4155844155844, Blast_Score=140, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 32660; Mature: 32660

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS00137 SUBTILASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCC
>Mature Secondary Structure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HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]