Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 71905989

GI number: 71905989

Start: 379624

End: 381165

Strand: Reverse

Name: pleD [H]

Synonym: Daro_0347

Alternate gene names: 71905989

Gene position: 381165-379624 (Counterclockwise)

Preceding gene: 71905990

Following gene: 71905943

Centisome position: 8.47

GC content: 54.6

Gene sequence:

>1542_bases
ATGATTCCAATAATGAACAAAATGAAGGTACTGGCGGTTGATGACAACCGCACCAACCTGCACATTCTCCAGGTCTTCCT
GAAGAAGCTTGGGCATGAAGTCGTTCTTGCTGAGAACGGCGAGGAGGCGGTACGTCGATTTGAGACCGACGCTCCGGACC
TGGTTCTCCTCGACATCATGATGCCGGTTATGGATGGCTTTGAAGCAGCTCGTCGTATCAAGGCCATGACCCGTGATCGC
TGGACGCCAGTCATCTTCCTTTCCGCCCTGAATCGCGACGAAAACCTCGTTGAAGGGTTGGATGCAGGCGCCGACGACTA
CCTTACCAAGCCGATCAATTTTGTCGTTCTGGAAGCCAAGTTGCGTTCGATGCAACGTTCGCTGACCATGCAGCAGCAGT
CTATCGATTCGCTGCGCCGGGTTCAGGCAATTTCCGACAACGTGCTGGATGCCATCGTTACAACCAACGAAGAAGGAATG
ATCGTCTCGGTAAATCAATCCACCGAACGTATTTTTGGTTGGTCCACGGCAGAGCTGCTTGGACAGAATGCCTCGCTACT
AATGCCAGTCCCGGCGAGCGCATCGGGTGACATAATGCTCAGCCCGGGTGACGCGGCAAGGCCCTTGCCGGGGGCTGGAC
AAGAACAGGAAATAAATGCCCGGCACAAGGATGGGCATATATTCCCGGCAACCATCTCAATCAGTCAGGTCGAGCTCGAC
AATCAACGCATGATCATCAGTGTGATCCGCGACATTTCCGAGCGGAAAGAAACCGAACAAAAGCTGCGCGAAAACGCCCA
GCAGCTGCAGGACTACTACGACCAAACCCAGACCGAACAACAACTTGCCCTGCGCCTGATGGAAAAGCAACTCCACCGCA
GCGGTCTTCGCGACAAACGCCTCCGCTACAAAGTCATTCCAGCCGAACATTTCAGCGGTGACATTGTCGCCGCCTCCCGC
TCCCCGGATGGACTCTTGTACGCGTTACTGGCCGATGCAACCGGCCATGGATTGACCGCCGCGATCAGTGTCCTGCCGGT
ATTGGCCCTGTTTTATCGGATGACCAAACTCAACCGGTCAATCCAGGAAATAGTGCTGGAATTAAATCAACAACTAAAGG
AATCCATGCCGGTCGGGCGTTTTGTCGCCGTGACGCTGGTCTGCCTAGATGAAAAGAACAGAAAAGGCGAAATATGGGTC
GGCGGTACGCCCGAAGCCTTCCTTTTTGATCGCTGGGGGCGGGTTGCCAGCACATTCCCTTCCGACAACCTGCCGCTCGG
CATCGTTGGCAGCAATGAACTAGGCGGCCACCCACGACTATTTTCGTGGGAACCGGAAAGCCAACTCGCGCTATGCTCCG
ATGGCCTTCTGGAAGCGACCGACACCGAAGGTAAACAGTTTGGAAATTCGGGCCTGATCAACTCCGCATCAAACACCTCG
CCAAGCGACCGCTTTGCCAAGATAGAAACTGCGCTGAAAGAGCATCAGAATGGCTGCGCCGCCAGCGACGATGTATCACT
AATGCTGATCGACTGCCCTTAA

Upstream 100 bases:

>100_bases
GGTTGATTGCCAAGTTTCGACAGCAACAGGGACGAAGGTTGGTAATATCGCAGCTAGCGATATTTAGCGCTTCAACGCGG
ATATCCCGCATTCGTCGATC

Downstream 100 bases:

>100_bases
TAAGAAATTGGCAGCCAAAGAAAAAGGCCGGAGAACCGGCCTTTTTTCTATTTGAGAATTTCTCAGCTTACTCAGCTGCA
TCCTCAATCTCAACCGCTTC

Product: PAS

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MIPIMNKMKVLAVDDNRTNLHILQVFLKKLGHEVVLAENGEEAVRRFETDAPDLVLLDIMMPVMDGFEAARRIKAMTRDR
WTPVIFLSALNRDENLVEGLDAGADDYLTKPINFVVLEAKLRSMQRSLTMQQQSIDSLRRVQAISDNVLDAIVTTNEEGM
IVSVNQSTERIFGWSTAELLGQNASLLMPVPASASGDIMLSPGDAARPLPGAGQEQEINARHKDGHIFPATISISQVELD
NQRMIISVIRDISERKETEQKLRENAQQLQDYYDQTQTEQQLALRLMEKQLHRSGLRDKRLRYKVIPAEHFSGDIVAASR
SPDGLLYALLADATGHGLTAAISVLPVLALFYRMTKLNRSIQEIVLELNQQLKESMPVGRFVAVTLVCLDEKNRKGEIWV
GGTPEAFLFDRWGRVASTFPSDNLPLGIVGSNELGGHPRLFSWEPESQLALCSDGLLEATDTEGKQFGNSGLINSASNTS
PSDRFAKIETALKEHQNGCAASDDVSLMLIDCP

Sequences:

>Translated_513_residues
MIPIMNKMKVLAVDDNRTNLHILQVFLKKLGHEVVLAENGEEAVRRFETDAPDLVLLDIMMPVMDGFEAARRIKAMTRDR
WTPVIFLSALNRDENLVEGLDAGADDYLTKPINFVVLEAKLRSMQRSLTMQQQSIDSLRRVQAISDNVLDAIVTTNEEGM
IVSVNQSTERIFGWSTAELLGQNASLLMPVPASASGDIMLSPGDAARPLPGAGQEQEINARHKDGHIFPATISISQVELD
NQRMIISVIRDISERKETEQKLRENAQQLQDYYDQTQTEQQLALRLMEKQLHRSGLRDKRLRYKVIPAEHFSGDIVAASR
SPDGLLYALLADATGHGLTAAISVLPVLALFYRMTKLNRSIQEIVLELNQQLKESMPVGRFVAVTLVCLDEKNRKGEIWV
GGTPEAFLFDRWGRVASTFPSDNLPLGIVGSNELGGHPRLFSWEPESQLALCSDGLLEATDTEGKQFGNSGLINSASNTS
PSDRFAKIETALKEHQNGCAASDDVSLMLIDCP
>Mature_513_residues
MIPIMNKMKVLAVDDNRTNLHILQVFLKKLGHEVVLAENGEEAVRRFETDAPDLVLLDIMMPVMDGFEAARRIKAMTRDR
WTPVIFLSALNRDENLVEGLDAGADDYLTKPINFVVLEAKLRSMQRSLTMQQQSIDSLRRVQAISDNVLDAIVTTNEEGM
IVSVNQSTERIFGWSTAELLGQNASLLMPVPASASGDIMLSPGDAARPLPGAGQEQEINARHKDGHIFPATISISQVELD
NQRMIISVIRDISERKETEQKLRENAQQLQDYYDQTQTEQQLALRLMEKQLHRSGLRDKRLRYKVIPAEHFSGDIVAASR
SPDGLLYALLADATGHGLTAAISVLPVLALFYRMTKLNRSIQEIVLELNQQLKESMPVGRFVAVTLVCLDEKNRKGEIWV
GGTPEAFLFDRWGRVASTFPSDNLPLGIVGSNELGGHPRLFSWEPESQLALCSDGLLEATDTEGKQFGNSGLINSASNTS
PSDRFAKIETALKEHQNGCAASDDVSLMLIDCP

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=116, Percent_Identity=37.9310344827586, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1786784, Length=119, Percent_Identity=36.9747899159664, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1786599, Length=152, Percent_Identity=31.5789473684211, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789149, Length=105, Percent_Identity=40, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787375, Length=150, Percent_Identity=32, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788550, Length=135, Percent_Identity=34.0740740740741, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87082012, Length=119, Percent_Identity=33.6134453781513, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786911, Length=119, Percent_Identity=36.9747899159664, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1788191, Length=125, Percent_Identity=34.4, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1789809, Length=118, Percent_Identity=32.2033898305085, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI2367329, Length=131, Percent_Identity=38.9312977099237, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI48994928, Length=124, Percent_Identity=35.4838709677419, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323034, Length=144, Percent_Identity=32.6388888888889, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 56873; Mature: 56873

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPIMNKMKVLAVDDNRTNLHILQVFLKKLGHEVVLAENGEEAVRRFETDAPDLVLLDIM
CCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCEEHHHHH
MPVMDGFEAARRIKAMTRDRWTPVIFLSALNRDENLVEGLDAGADDYLTKPINFVVLEAK
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCCCCHHHCCCCEEEEEHHH
LRSMQRSLTMQQQSIDSLRRVQAISDNVLDAIVTTNEEGMIVSVNQSTERIFGWSTAELL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCHHHCCCCHHHHH
GQNASLLMPVPASASGDIMLSPGDAARPLPGAGQEQEINARHKDGHIFPATISISQVELD
CCCCCEEEECCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEEC
NQRMIISVIRDISERKETEQKLRENAQQLQDYYDQTQTEQQLALRLMEKQLHRSGLRDKR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LRYKVIPAEHFSGDIVAASRSPDGLLYALLADATGHGLTAAISVLPVLALFYRMTKLNRS
CEEEEECHHCCCCCEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IQEIVLELNQQLKESMPVGRFVAVTLVCLDEKNRKGEIWVGGTPEAFLFDRWGRVASTFP
HHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCEEECCCCCHHHHHHHHHHHHCCC
SDNLPLGIVGSNELGGHPRLFSWEPESQLALCSDGLLEATDTEGKQFGNSGLINSASNTS
CCCCCEEEEECCCCCCCCCEECCCCCCCEEECCCCCEECCCCCCHHCCCCCCCCCCCCCC
PSDRFAKIETALKEHQNGCAASDDVSLMLIDCP
CHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MIPIMNKMKVLAVDDNRTNLHILQVFLKKLGHEVVLAENGEEAVRRFETDAPDLVLLDIM
CCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCEEHHHHH
MPVMDGFEAARRIKAMTRDRWTPVIFLSALNRDENLVEGLDAGADDYLTKPINFVVLEAK
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCCCCHHHCCCCEEEEEHHH
LRSMQRSLTMQQQSIDSLRRVQAISDNVLDAIVTTNEEGMIVSVNQSTERIFGWSTAELL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCHHHCCCCHHHHH
GQNASLLMPVPASASGDIMLSPGDAARPLPGAGQEQEINARHKDGHIFPATISISQVELD
CCCCCEEEECCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEEC
NQRMIISVIRDISERKETEQKLRENAQQLQDYYDQTQTEQQLALRLMEKQLHRSGLRDKR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LRYKVIPAEHFSGDIVAASRSPDGLLYALLADATGHGLTAAISVLPVLALFYRMTKLNRS
CEEEEECHHCCCCCEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IQEIVLELNQQLKESMPVGRFVAVTLVCLDEKNRKGEIWVGGTPEAFLFDRWGRVASTFP
HHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCEEECCCCCHHHHHHHHHHHHCCC
SDNLPLGIVGSNELGGHPRLFSWEPESQLALCSDGLLEATDTEGKQFGNSGLINSASNTS
CCCCCEEEEECCCCCCCCCEECCCCCCCEEECCCCCEECCCCCCHHCCCCCCCCCCCCCC
PSDRFAKIETALKEHQNGCAASDDVSLMLIDCP
CHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA