BacMap

Definition	Dechloromonas aromatica RCB, complete genome.
Accession	NC_007298
Length	4,501,104

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The map label for this gene is fadB1 [H]

Identifier: 71905818

GI number: 71905818

Start: 192703

End: 193476

Strand: Reverse

Name: fadB1 [H]

Synonym: Daro_0176

Alternate gene names: 71905818

Gene position: 193476-192703 (Counterclockwise)

Preceding gene: 71905820

Following gene: 71905817

Centisome position: 4.3

GC content: 60.85

Gene sequence:

>774_bases
ATGACCCAAGTAGTCCTCACCGAAGTGATCGGCAAGGTCGGCCTGATCCGCATCAACCGTCCGGAAGCGATGAACGCACT
GAACAACGACGTCGTCGACGGCATCGGTGCCGCCATCGACATCTACGAAGCCGACGAGAACATCGGCTGCATCGTCATCA
CCGGCAATGAGAAAGCCTTCGCCGCCGGAGCCGACATCGGCTTCATGAAGGATTTCGACTACATGCACGCCTACAAGACC
GACTTCATCACCCGCAACTGGGAACGCATCAAGACCACCCGCAAGCCGGTCATCGCCGCAGTGTCCGGCTTTGCCCTCGG
TGGTGGCTGCGAAATGGCGATGATGTGCGACATGATCTTCGCCGCCGATACCGCCAAGTTCGGCCAACCGGAAATTCGCC
TTGGCACCATGCCCGGGGCCGGTGGCACCCAGAGATTGCCCCGCGCCGTCGGCAAGGCCAAGGCGATGGACATGTGCCTG
ACGGCCCGCATGATGGATGCGGCCGAAGCTGAAAAGGCCGGCCTGGTCGCCCGCATCATCCCGGCCGACCAATTGCTCGA
CGAAACCCTGAAAGCCGCCCAGACCATTGCCGGCTATTCGCTGCCGGTGGTCATGATGATCAAGGAGTCGGTCAATCGCG
CCTATGAATCCTCGCTGAACGAAGGCCTGCTCTTTGAGCGCCGCGTCTTCCATTCGGCCTTCGCCCTCGAGGACCAGAAG
GAAGGCATGGCTGCCTTTGTCGAGAAGCGCAAGCCCGTATTCAAGAACCGCTGA

Upstream 100 bases:

>100_bases
GCGGCCAAGACTGTCGTCAGGCGGACAAACCGGGATTTAGCTGCTGCTAATATCGTGGTTTGTCGCCATCAATAAAATCC
CACAGGCAAGGAGAGCCAGC

Downstream 100 bases:

>100_bases
GATCAGCCTTCCCAAGAAATCCGGCCGATGCATCTATTCGTCGACATTTCCAGCCACGGCTTCGGGCACCTCGCCATCAC
GGCGCCGGTGCTCAACGCCC

Product: short chain enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MTQVVLTEVIGKVGLIRINRPEAMNALNNDVVDGIGAAIDIYEADENIGCIVITGNEKAFAAGADIGFMKDFDYMHAYKT
DFITRNWERIKTTRKPVIAAVSGFALGGGCEMAMMCDMIFAADTAKFGQPEIRLGTMPGAGGTQRLPRAVGKAKAMDMCL
TARMMDAAEAEKAGLVARIIPADQLLDETLKAAQTIAGYSLPVVMMIKESVNRAYESSLNEGLLFERRVFHSAFALEDQK
EGMAAFVEKRKPVFKNR

Sequences:

>Translated_257_residues
MTQVVLTEVIGKVGLIRINRPEAMNALNNDVVDGIGAAIDIYEADENIGCIVITGNEKAFAAGADIGFMKDFDYMHAYKT
DFITRNWERIKTTRKPVIAAVSGFALGGGCEMAMMCDMIFAADTAKFGQPEIRLGTMPGAGGTQRLPRAVGKAKAMDMCL
TARMMDAAEAEKAGLVARIIPADQLLDETLKAAQTIAGYSLPVVMMIKESVNRAYESSLNEGLLFERRVFHSAFALEDQK
EGMAAFVEKRKPVFKNR
>Mature_256_residues
TQVVLTEVIGKVGLIRINRPEAMNALNNDVVDGIGAAIDIYEADENIGCIVITGNEKAFAAGADIGFMKDFDYMHAYKTD
FITRNWERIKTTRKPVIAAVSGFALGGGCEMAMMCDMIFAADTAKFGQPEIRLGTMPGAGGTQRLPRAVGKAKAMDMCLT
ARMMDAAEAEKAGLVARIIPADQLLDETLKAAQTIAGYSLPVVMMIKESVNRAYESSLNEGLLFERRVFHSAFALEDQKE
GMAAFVEKRKPVFKNR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=258, Percent_Identity=50.3875968992248, Blast_Score=268, Evalue=2e-72,
Organism=Homo sapiens, GI31542718, Length=245, Percent_Identity=29.3877551020408, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI68989263, Length=236, Percent_Identity=33.4745762711864, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI4502327, Length=247, Percent_Identity=34.412955465587, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI20127408, Length=178, Percent_Identity=35.3932584269663, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI70995211, Length=250, Percent_Identity=30, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI150378541, Length=244, Percent_Identity=29.0983606557377, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI37594471, Length=210, Percent_Identity=26.6666666666667, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI37594469, Length=210, Percent_Identity=26.6666666666667, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI25453481, Length=262, Percent_Identity=22.9007633587786, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI51538573, Length=262, Percent_Identity=22.9007633587786, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI4757968, Length=257, Percent_Identity=23.3463035019455, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI49355787, Length=257, Percent_Identity=23.3463035019455, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI4757966, Length=249, Percent_Identity=22.8915662650602, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI51538581, Length=249, Percent_Identity=22.8915662650602, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI261878539, Length=135, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI157694516, Length=242, Percent_Identity=26.4462809917355, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI213417737, Length=242, Percent_Identity=26.4462809917355, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI221136756, Length=186, Percent_Identity=26.8817204301075, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI221136753, Length=186, Percent_Identity=26.8817204301075, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI221307494, Length=186, Percent_Identity=26.8817204301075, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=246, Percent_Identity=43.0894308943089, Blast_Score=205, Evalue=2e-54,
Organism=Escherichia coli, GI221142681, Length=255, Percent_Identity=34.1176470588235, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1788597, Length=254, Percent_Identity=32.6771653543307, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1787660, Length=265, Percent_Identity=30.188679245283, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI1788682, Length=222, Percent_Identity=32.8828828828829, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1790281, Length=176, Percent_Identity=32.9545454545455, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87082183, Length=256, Percent_Identity=30.078125, Blast_Score=98, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17554946, Length=245, Percent_Identity=51.8367346938776, Blast_Score=271, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=47.1311475409836, Blast_Score=239, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI17560910, Length=253, Percent_Identity=32.0158102766798, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17540714, Length=234, Percent_Identity=29.0598290598291, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI25144276, Length=214, Percent_Identity=35.0467289719626, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17508953, Length=214, Percent_Identity=35.0467289719626, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17508951, Length=214, Percent_Identity=35.0467289719626, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17558304, Length=182, Percent_Identity=34.6153846153846, Blast_Score=100, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17540306, Length=240, Percent_Identity=29.1666666666667, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17534483, Length=256, Percent_Identity=26.5625, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=258, Percent_Identity=27.906976744186, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=219, Percent_Identity=29.2237442922374, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17535521, Length=237, Percent_Identity=27.4261603375527, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25144157, Length=193, Percent_Identity=25.9067357512953, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25144160, Length=193, Percent_Identity=25.9067357512953, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI25141363, Length=197, Percent_Identity=26.3959390862944, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=194, Percent_Identity=26.8041237113402, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20129971, Length=257, Percent_Identity=54.0856031128405, Blast_Score=288, Evalue=3e-78,
Organism=Drosophila melanogaster, GI24653477, Length=257, Percent_Identity=54.0856031128405, Blast_Score=288, Evalue=3e-78,
Organism=Drosophila melanogaster, GI24653139, Length=251, Percent_Identity=35.4581673306773, Blast_Score=134, Evalue=8e-32,
Organism=Drosophila melanogaster, GI21357171, Length=253, Percent_Identity=29.2490118577075, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24650670, Length=213, Percent_Identity=30.5164319248826, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19922422, Length=256, Percent_Identity=25.78125, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19920382, Length=255, Percent_Identity=30.1960784313725, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI19921000, Length=182, Percent_Identity=34.0659340659341, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24583077, Length=182, Percent_Identity=34.0659340659341, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24583079, Length=182, Percent_Identity=34.0659340659341, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24654903, Length=187, Percent_Identity=27.807486631016, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571729, Length=195, Percent_Identity=28.7179487179487, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28571730, Length=195, Percent_Identity=28.7179487179487, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28000; Mature: 27869

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQVVLTEVIGKVGLIRINRPEAMNALNNDVVDGIGAAIDIYEADENIGCIVITGNEKAF
CCHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCCEE
AAGADIGFMKDFDYMHAYKTDFITRNWERIKTTRKPVIAAVSGFALGGGCEMAMMCDMIF
ECCCCCCHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCEEECCCCHHHHHHHHHH
AADTAKFGQPEIRLGTMPGAGGTQRLPRAVGKAKAMDMCLTARMMDAAEAEKAGLVARII
HHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
PADQLLDETLKAAQTIAGYSLPVVMMIKESVNRAYESSLNEGLLFERRVFHSAFALEDQK
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHH
EGMAAFVEKRKPVFKNR
HHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TQVVLTEVIGKVGLIRINRPEAMNALNNDVVDGIGAAIDIYEADENIGCIVITGNEKAF
CHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCCEE
AAGADIGFMKDFDYMHAYKTDFITRNWERIKTTRKPVIAAVSGFALGGGCEMAMMCDMIF
ECCCCCCHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCEEECCCCHHHHHHHHHH
AADTAKFGQPEIRLGTMPGAGGTQRLPRAVGKAKAMDMCLTARMMDAAEAEKAGLVARII
HHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
PADQLLDETLKAAQTIAGYSLPVVMMIKESVNRAYESSLNEGLLFERRVFHSAFALEDQK
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHH
EGMAAFVEKRKPVFKNR
HHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]