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The map label for this gene is thlA [H]

Identifier: 71905740

GI number: 71905740

Start: 114507

End: 115697

Strand: Reverse

Name: thlA [H]

Synonym: Daro_0098

Alternate gene names: 71905740

Gene position: 115697-114507 (Counterclockwise)

Preceding gene: 71905741

Following gene: 71905739

Centisome position: 2.57

GC content: 66.16

Gene sequence:

>1191_bases
ATGAATGACCCGATCGTTATCGTTTCCGCCGCCCGTACCGCCATGGGCGCCTTTCAGGGCGGCTTTGCCGGCCTGACCGC
CGCCAATCTCGGCGCCGTGGCCATCAAGGCCGCTGTCGAGCGCGCCGGCATCGACGCCAACCTGATCGAAGAAGTGCTGA
TGGGCTGCGTGCTGCAAGCCGGCCAGGGCCAGGCTCCGGCCCGCCAGTCCGCGCTGCAGGCCGGCCTGCCCCTCTCCGCC
GGTTGCGCCACCATCCACAAGGTTTGCGGCTCAGCCATGAAGGCGACCATGCTCGGCCACGACGGCATCCTGGCCGGTTC
CTACGGTGCGGCCGTGGTGGGCGGCATGGAGTCGATGACCAATGCCCCCTACCTGCTGCCCAAGGCCCGCGGTGGTTACC
GCCTCGGCCACGGCCAGCTGATGGACCACATGTTCCTCGATGGCCTGGAAGATTCTTACGGCAAGGAAACCCGCGGCCGC
CTGATGGGCACCTTTGCCGAAGAATGCGCTGCCAGCTACGGCTTCACCCGTGAAGCGCAGGACGAATTTGCTGTCCGCTC
GACCACCCGTGCCATCGAAGCCAGCAACAACGGCAGCTTCGCCTGGGAAATCGCCCCGACCACCGTCGCCGGCCGCAAAG
GCGACGTCGTGATCGACAAGGACGAAGGCCCGTTTGCCGTCAATGTCGAAAAAATCCCGACCCTGAAGCCGGCTTTCAAG
AAAGATGGCACGGTCACCGCCGCCAACTCCTCGTCGATTTCCGACGGCGCCGCCGCGATGGTCCTGATGCGCGCCTCGCA
AGCCGCCAAGCTCGGCCTGCAACCGATTGCCCGCATCGTCGGCCATACGACGCATGCCGGTACGCCCGCCCTGTTCCCGT
CGGCCCCGGTCGGCGCCATGCAGAAGCTGTTCGCCAAGACCGGCTGGACCGCTGAGTCGGTCGATCTGTATGAAATCAAC
GAAGCCTTCGCCGTCGTCACCATGGCCTCGCTGCATGACCTCAAGCTCGACCCGGCCAAGGTCAACATCCACGGTGGCGC
CTGCGCGCTGGGCCACCCGATCGGCGCCTCCGGTGCCCGCATCGTCGTCACGCTGTTGGGCGCCCTGAAGAAGTACGGCA
AGAAGCGCGGCGTGGCCAGCCTGTGCATCGGCGGTGGCGAAGCAACGGCTCTCGCGGTGGAAATGCTGTAA

Upstream 100 bases:

>100_bases
GGCATCACGATCGATTGCCCACAGGATTTGCCTTCCCGCTACGACGCCGCACTCAAAGCGTTGGACGCCTAGTTACAACA
ACCACTCAGGAGAAATCAGC

Downstream 100 bases:

>100_bases
TTGCCGCAAAAGGCCCTTTCGCTCACTTACGCCAAACTGGTCGCTGCGATGTTCATGTGGGGAGGCACCTGGATCGCCGG
GCGCATCATCGCGCAGGAGC

Product: thiolase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MNDPIVIVSAARTAMGAFQGGFAGLTAANLGAVAIKAAVERAGIDANLIEEVLMGCVLQAGQGQAPARQSALQAGLPLSA
GCATIHKVCGSAMKATMLGHDGILAGSYGAAVVGGMESMTNAPYLLPKARGGYRLGHGQLMDHMFLDGLEDSYGKETRGR
LMGTFAEECAASYGFTREAQDEFAVRSTTRAIEASNNGSFAWEIAPTTVAGRKGDVVIDKDEGPFAVNVEKIPTLKPAFK
KDGTVTAANSSSISDGAAAMVLMRASQAAKLGLQPIARIVGHTTHAGTPALFPSAPVGAMQKLFAKTGWTAESVDLYEIN
EAFAVVTMASLHDLKLDPAKVNIHGGACALGHPIGASGARIVVTLLGALKKYGKKRGVASLCIGGGEATALAVEML

Sequences:

>Translated_396_residues
MNDPIVIVSAARTAMGAFQGGFAGLTAANLGAVAIKAAVERAGIDANLIEEVLMGCVLQAGQGQAPARQSALQAGLPLSA
GCATIHKVCGSAMKATMLGHDGILAGSYGAAVVGGMESMTNAPYLLPKARGGYRLGHGQLMDHMFLDGLEDSYGKETRGR
LMGTFAEECAASYGFTREAQDEFAVRSTTRAIEASNNGSFAWEIAPTTVAGRKGDVVIDKDEGPFAVNVEKIPTLKPAFK
KDGTVTAANSSSISDGAAAMVLMRASQAAKLGLQPIARIVGHTTHAGTPALFPSAPVGAMQKLFAKTGWTAESVDLYEIN
EAFAVVTMASLHDLKLDPAKVNIHGGACALGHPIGASGARIVVTLLGALKKYGKKRGVASLCIGGGEATALAVEML
>Mature_396_residues
MNDPIVIVSAARTAMGAFQGGFAGLTAANLGAVAIKAAVERAGIDANLIEEVLMGCVLQAGQGQAPARQSALQAGLPLSA
GCATIHKVCGSAMKATMLGHDGILAGSYGAAVVGGMESMTNAPYLLPKARGGYRLGHGQLMDHMFLDGLEDSYGKETRGR
LMGTFAEECAASYGFTREAQDEFAVRSTTRAIEASNNGSFAWEIAPTTVAGRKGDVVIDKDEGPFAVNVEKIPTLKPAFK
KDGTVTAANSSSISDGAAAMVLMRASQAAKLGLQPIARIVGHTTHAGTPALFPSAPVGAMQKLFAKTGWTAESVDLYEIN
EAFAVVTMASLHDLKLDPAKVNIHGGACALGHPIGASGARIVVTLLGALKKYGKKRGVASLCIGGGEATALAVEML

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4557237, Length=394, Percent_Identity=45.9390862944162, Blast_Score=322, Evalue=3e-88,
Organism=Homo sapiens, GI148539872, Length=396, Percent_Identity=44.9494949494949, Blast_Score=318, Evalue=6e-87,
Organism=Homo sapiens, GI167614485, Length=392, Percent_Identity=34.9489795918367, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI4501853, Length=401, Percent_Identity=36.1596009975062, Blast_Score=206, Evalue=4e-53,
Organism=Homo sapiens, GI4504327, Length=421, Percent_Identity=29.4536817102138, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI194353979, Length=397, Percent_Identity=27.2040302267003, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1788554, Length=393, Percent_Identity=49.8727735368957, Blast_Score=329, Evalue=2e-91,
Organism=Escherichia coli, GI87082165, Length=395, Percent_Identity=42.5316455696203, Blast_Score=301, Evalue=4e-83,
Organism=Escherichia coli, GI48994986, Length=404, Percent_Identity=37.3762376237624, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1787663, Length=400, Percent_Identity=34.25, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI1788683, Length=402, Percent_Identity=28.8557213930348, Blast_Score=150, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI25147385, Length=395, Percent_Identity=40.7594936708861, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17535921, Length=399, Percent_Identity=41.8546365914787, Blast_Score=283, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI133906874, Length=391, Percent_Identity=37.0843989769821, Blast_Score=254, Evalue=5e-68,
Organism=Caenorhabditis elegans, GI17535917, Length=405, Percent_Identity=31.8518518518519, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17551802, Length=422, Percent_Identity=31.042654028436, Blast_Score=170, Evalue=9e-43,
Organism=Saccharomyces cerevisiae, GI6325229, Length=402, Percent_Identity=46.0199004975124, Blast_Score=320, Evalue=2e-88,
Organism=Saccharomyces cerevisiae, GI6322031, Length=393, Percent_Identity=34.8600508905852, Blast_Score=196, Evalue=8e-51,
Organism=Drosophila melanogaster, GI24655093, Length=397, Percent_Identity=47.103274559194, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24640423, Length=394, Percent_Identity=47.2081218274112, Blast_Score=328, Evalue=4e-90,
Organism=Drosophila melanogaster, GI17648125, Length=394, Percent_Identity=36.0406091370558, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI17137578, Length=421, Percent_Identity=30.8788598574822, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40599; Mature: 40599

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDPIVIVSAARTAMGAFQGGFAGLTAANLGAVAIKAAVERAGIDANLIEEVLMGCVLQA
CCCCEEEEECHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
GQGQAPARQSALQAGLPLSAGCATIHKVCGSAMKATMLGHDGILAGSYGAAVVGGMESMT
CCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHCCHHHHC
NAPYLLPKARGGYRLGHGQLMDHMFLDGLEDSYGKETRGRLMGTFAEECAASYGFTREAQ
CCCEEEECCCCCEEECHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHCCCCCCCH
DEFAVRSTTRAIEASNNGSFAWEIAPTTVAGRKGDVVIDKDEGPFAVNVEKIPTLKPAFK
HHHHHHHHHHHEEECCCCCEEEEECCCEECCCCCCEEEECCCCCEEEEHHCCCCCCCCCC
KDGTVTAANSSSISDGAAAMVLMRASQAAKLGLQPIARIVGHTTHAGTPALFPSAPVGAM
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCHHHH
QKLFAKTGWTAESVDLYEINEAFAVVTMASLHDLKLDPAKVNIHGGACALGHPIGASGAR
HHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEECCCEEECCCCCCCCCHH
IVVTLLGALKKYGKKRGVASLCIGGGEATALAVEML
HHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEEC
>Mature Secondary Structure
MNDPIVIVSAARTAMGAFQGGFAGLTAANLGAVAIKAAVERAGIDANLIEEVLMGCVLQA
CCCCEEEEECHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
GQGQAPARQSALQAGLPLSAGCATIHKVCGSAMKATMLGHDGILAGSYGAAVVGGMESMT
CCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHCCHHHHC
NAPYLLPKARGGYRLGHGQLMDHMFLDGLEDSYGKETRGRLMGTFAEECAASYGFTREAQ
CCCEEEECCCCCEEECHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHCCCCCCCH
DEFAVRSTTRAIEASNNGSFAWEIAPTTVAGRKGDVVIDKDEGPFAVNVEKIPTLKPAFK
HHHHHHHHHHHEEECCCCCEEEEECCCEECCCCCCEEEECCCCCEEEEHHCCCCCCCCCC
KDGTVTAANSSSISDGAAAMVLMRASQAAKLGLQPIARIVGHTTHAGTPALFPSAPVGAM
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCHHHH
QKLFAKTGWTAESVDLYEINEAFAVVTMASLHDLKLDPAKVNIHGGACALGHPIGASGAR
HHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEECCCEEECCCCCCCCCHH
IVVTLLGALKKYGKKRGVASLCIGGGEATALAVEML
HHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]