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The map label for this gene is glnT [H]

Identifier: 71905719

GI number: 71905719

Start: 94510

End: 95871

Strand: Reverse

Name: glnT [H]

Synonym: Daro_0077

Alternate gene names: 71905719

Gene position: 95871-94510 (Counterclockwise)

Preceding gene: 71905720

Following gene: 71905718

Centisome position: 2.13

GC content: 61.67

Gene sequence:

>1362_bases
ATGAACAGCAAAGAAGATCTCGCCCGCCTGCAGCAGGAACTGGAGGCCAAAGGCGTCAAATACTGCATCGGCGCCTACGT
CGACATCCACGGCGTGCCCAAGGCCAAGGTGGTGCCCATCCACCATCTCCTGCACATGGCCAAAGGCTCCGAACGCTATA
CCGGTTATGCCCTCGACGGCCTCGGCCAGGCGCCGAACGACGATGAACTGACCTCCATCCCGGACTTTTCCCGGCTCATC
CAGCTGCCCTGGGAACCGAAAATCGCCTGGATTCCGTCCGACAATCATTTTCAGGGCAAGCCCTACCCCCTGAACACGCG
TGTTGCCCTGCAAAACCAGCTGGCCGAAGCCGCCAAACTGGGCTTCGGCATGAATCTCGGCATCGAATGCGAGATTTTCC
TGCTCCGCCAGAACGCCGATGGCAGCCTGAGCGTACCCGACCCCGAAGACAAGCTGGCCAAGGCCTGCTATGACGTGCGC
GGTTTCACCAACAATTTCAGCTGGCTCGACAAGGTGGCGACGGCGATCAACGATCTCGGCTGGGATCTCTATTCCTTCGA
CCACGAGGACGCCAACGGCCAGTTCGAGTTCGACTTCCAGTACGCCGACGCGCTGACCACCTGCGACCGCCTGACCTTCT
TCCGCTACATGGCCAAGCACTATGCCCGCGAGGAAGGTCTGCTGGCGACGATGATGCCCAAGCCCTTCGCCGACAAGACC
GGCAACGGCGCGCATTTCAACATGTCGCTCTATGATCTGCAGGATGGTCGCAACCTGTTTGCCTGCGATCCCAAGGACGA
CCCGAACGGCATCGGCCTGACCAAACTCGGCTACCACTTCGTCGCTGGCATCCTGAAGCATGGTCGGGCACTGTGCGCGG
TCTTCGCGCCGACGGTCAACAGCTACAAGCGCCTGGTCCGCCGTGGCGCGATGAACTATTTCTCGTGGGCGCCGGTATTC
AACTCCTACGGCTCGAACAACCGCACCAATTCGGTCCGCATTCCGTCCGGTGGCGGTCGTTGCGAGTCACGCAACGCCGA
CGGCGCGGTCAATCCCTACCTGGTGGCGACGCTGGTGCTGGCGGCCGGCCTCGAAGGCATCCGCGAAGGCCTCGATCCGG
GCAAGCCGAACGAGGACAACCTGTATGCGATCAGCGAAGCCGAACGCCACGCCCGCGGCATCGAATTCCTGCCGCAGACA
CTGCAGGAGGCCGTCGCCGCCTTCGCCGCCGACCCGCTGGTCGAGCAGACCCTCGGCAAGGAGTTGCGTGACGAGTTCAT
CCGCTACAAGAGCGAAGAATGGGAGGCGTATCATCTGACCGTCAGCCAGTGGGAAATCGACCGCTACAGCTACATGTTCT
GA

Upstream 100 bases:

>100_bases
GATCGCGCGCGGTTGCCAGGAAACACCTCCCCTCGATTTTTCCTATTTCAGTCAGGCCTGACGAAATACCTCTGCCCCCC
ATCGCTCTCAAGGATCGAAC

Downstream 100 bases:

>100_bases
GCCGCCACACCCACACAGGAGCCTGCCATGGCCGATGAGGATAAGAATACATCCGTCAGTCGCATCAGCATCTCGCTGCC
CCCCGCCCTGCTCGACGAGC

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase; Glutamine synthetase III; GSIII [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MNSKEDLARLQQELEAKGVKYCIGAYVDIHGVPKAKVVPIHHLLHMAKGSERYTGYALDGLGQAPNDDELTSIPDFSRLI
QLPWEPKIAWIPSDNHFQGKPYPLNTRVALQNQLAEAAKLGFGMNLGIECEIFLLRQNADGSLSVPDPEDKLAKACYDVR
GFTNNFSWLDKVATAINDLGWDLYSFDHEDANGQFEFDFQYADALTTCDRLTFFRYMAKHYAREEGLLATMMPKPFADKT
GNGAHFNMSLYDLQDGRNLFACDPKDDPNGIGLTKLGYHFVAGILKHGRALCAVFAPTVNSYKRLVRRGAMNYFSWAPVF
NSYGSNNRTNSVRIPSGGGRCESRNADGAVNPYLVATLVLAAGLEGIREGLDPGKPNEDNLYAISEAERHARGIEFLPQT
LQEAVAAFAADPLVEQTLGKELRDEFIRYKSEEWEAYHLTVSQWEIDRYSYMF

Sequences:

>Translated_453_residues
MNSKEDLARLQQELEAKGVKYCIGAYVDIHGVPKAKVVPIHHLLHMAKGSERYTGYALDGLGQAPNDDELTSIPDFSRLI
QLPWEPKIAWIPSDNHFQGKPYPLNTRVALQNQLAEAAKLGFGMNLGIECEIFLLRQNADGSLSVPDPEDKLAKACYDVR
GFTNNFSWLDKVATAINDLGWDLYSFDHEDANGQFEFDFQYADALTTCDRLTFFRYMAKHYAREEGLLATMMPKPFADKT
GNGAHFNMSLYDLQDGRNLFACDPKDDPNGIGLTKLGYHFVAGILKHGRALCAVFAPTVNSYKRLVRRGAMNYFSWAPVF
NSYGSNNRTNSVRIPSGGGRCESRNADGAVNPYLVATLVLAAGLEGIREGLDPGKPNEDNLYAISEAERHARGIEFLPQT
LQEAVAAFAADPLVEQTLGKELRDEFIRYKSEEWEAYHLTVSQWEIDRYSYMF
>Mature_453_residues
MNSKEDLARLQQELEAKGVKYCIGAYVDIHGVPKAKVVPIHHLLHMAKGSERYTGYALDGLGQAPNDDELTSIPDFSRLI
QLPWEPKIAWIPSDNHFQGKPYPLNTRVALQNQLAEAAKLGFGMNLGIECEIFLLRQNADGSLSVPDPEDKLAKACYDVR
GFTNNFSWLDKVATAINDLGWDLYSFDHEDANGQFEFDFQYADALTTCDRLTFFRYMAKHYAREEGLLATMMPKPFADKT
GNGAHFNMSLYDLQDGRNLFACDPKDDPNGIGLTKLGYHFVAGILKHGRALCAVFAPTVNSYKRLVRRGAMNYFSWAPVF
NSYGSNNRTNSVRIPSGGGRCESRNADGAVNPYLVATLVLAAGLEGIREGLDPGKPNEDNLYAISEAERHARGIEFLPQT
LQEAVAAFAADPLVEQTLGKELRDEFIRYKSEEWEAYHLTVSQWEIDRYSYMF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family. Type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=448, Percent_Identity=22.5446428571429, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1790301, Length=287, Percent_Identity=31.7073170731707, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI87081870, Length=461, Percent_Identity=25.1626898047722, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR017536 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 50766; Mature: 50766

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSKEDLARLQQELEAKGVKYCIGAYVDIHGVPKAKVVPIHHLLHMAKGSERYTGYALDG
CCCHHHHHHHHHHHHHCCHHEEEEEEEEECCCCCCEEEHHHHHHHHHCCCCCCCCEEECC
LGQAPNDDELTSIPDFSRLIQLPWEPKIAWIPSDNHFQGKPYPLNTRVALQNQLAEAAKL
CCCCCCCCCCCCCCCHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GFGMNLGIECEIFLLRQNADGSLSVPDPEDKLAKACYDVRGFTNNFSWLDKVATAINDLG
CCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
WDLYSFDHEDANGQFEFDFQYADALTTCDRLTFFRYMAKHYAREEGLLATMMPKPFADKT
CHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
GNGAHFNMSLYDLQDGRNLFACDPKDDPNGIGLTKLGYHFVAGILKHGRALCAVFAPTVN
CCCEEEEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCHHEEEHHHHHHH
SYKRLVRRGAMNYFSWAPVFNSYGSNNRTNSVRIPSGGGRCESRNADGAVNPYLVATLVL
HHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
AAGLEGIREGLDPGKPNEDNLYAISEAERHARGIEFLPQTLQEAVAAFAADPLVEQTLGK
HHHHHHHHHCCCCCCCCCCCEEEECHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
ELRDEFIRYKSEEWEAYHLTVSQWEIDRYSYMF
HHHHHHHHHCCCCCEEEEEEEEEEECCHHHCCC
>Mature Secondary Structure
MNSKEDLARLQQELEAKGVKYCIGAYVDIHGVPKAKVVPIHHLLHMAKGSERYTGYALDG
CCCHHHHHHHHHHHHHCCHHEEEEEEEEECCCCCCEEEHHHHHHHHHCCCCCCCCEEECC
LGQAPNDDELTSIPDFSRLIQLPWEPKIAWIPSDNHFQGKPYPLNTRVALQNQLAEAAKL
CCCCCCCCCCCCCCCHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GFGMNLGIECEIFLLRQNADGSLSVPDPEDKLAKACYDVRGFTNNFSWLDKVATAINDLG
CCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
WDLYSFDHEDANGQFEFDFQYADALTTCDRLTFFRYMAKHYAREEGLLATMMPKPFADKT
CHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
GNGAHFNMSLYDLQDGRNLFACDPKDDPNGIGLTKLGYHFVAGILKHGRALCAVFAPTVN
CCCEEEEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCHHEEEHHHHHHH
SYKRLVRRGAMNYFSWAPVFNSYGSNNRTNSVRIPSGGGRCESRNADGAVNPYLVATLVL
HHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
AAGLEGIREGLDPGKPNEDNLYAISEAERHARGIEFLPQTLQEAVAAFAADPLVEQTLGK
HHHHHHHHHCCCCCCCCCCCEEEECHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
ELRDEFIRYKSEEWEAYHLTVSQWEIDRYSYMF
HHHHHHHHHCCCCCEEEEEEEEEEECCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1356885 [H]