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The map label for this gene is ykoW [H]

Identifier: 71905700

GI number: 71905700

Start: 66585

End: 68936

Strand: Reverse

Name: ykoW [H]

Synonym: Daro_0058

Alternate gene names: 71905700

Gene position: 68936-66585 (Counterclockwise)

Preceding gene: 71905701

Following gene: 71905699

Centisome position: 1.53

GC content: 61.35

Gene sequence:

>2352_bases
ATGACCTTGATGAAACTCCCCCGTTCGCTGCGCACCCGCCTGATTACGCTGAGCATCATCGTCGAGATCGTGATGATCTC
GGCCATCGTCTGGAACAGCCAGCGCCTGATCGAGGCGCATCTGGTCCGCCAGTTTGAACTGCGCCGTGCTGAAATCAGCT
TGTTGCTGCAAGCCGCGCTGGGCGCGGCCATGGTCCAGCGCGACTATGCCGCAGTCAGCGAAACACTGACCAGCGCCCAG
CAAATGCAGGGCATGGTCTACCTGATCATGTTCGATGAACGCGGCCAGCGTATTGCCGCTGCCAATGCCGAACATGACAT
AAAGCCGGCCGGGGTGCGTGATTTCCGCGTTCCGGTGAGCGTCGAAAAACGCCCCTACGGCGAGCTTCAGGTCGGTATCG
ATCTGCGCTTTCTCGAAGAGGCAAGACAGGAAATCCTGTTCCAGAATATCGCGCTCGCCCTGGTCGGCATCCTGACCTCG
TCGGTCGTCCTTGGCGGCATTGCCTTGTGGCTGACGCGCCGCCTTGGCCGGCTGGTCAAGGCCAGCCACGATCTGGCTGA
TGGGCGGGGTTTCAAACCAATGCCTGCTAGCAGCAACGACGACATCGGCCAAGTCATCAGCGCCTTCAATGGCATGGCAA
AAACACTCGACCGCCGGGTCAGGGATCTGCAGGAAGCCGAAGCTCGGCAAAGGGCACTGCTCGAAACGGTCGATGCGGAG
CGTTCGCGACTCGACGCGCTGCTTTCGGCGATGCGTCTGGGACTGGTTTTTGTCGATCAGGACGAACGGATCTGCTATCT
GAACCCGGCCTTCGCCAGCCTCTGGTCCGTCAGTCTGGACGGTCATCTCGCCAATCTGCCGCTGGCCGATCTGCGCCGCT
GGCTGGATGCCGTACTGACCAACGGTGGCCAAAGCCAGCTGTTCAGGGGTGACGGCACCAGTGTGGAACTGGCCTTGCAC
GAAGGCCGAGTCATCACCCAGCAAGGGATAGCAGTCACCAGCGCCAGCGGTAGCGAGTTGGGACGTCTGTGGATTTTCGA
GGACGTGACCAACGAGCGACAACTGGCCGAACATCTGCTGTTCATGGCCGAGCGTGACCCGCTGACCGGACTCGCCAACC
GCGCCCGTTTTGCCGCCGAACTGGACAGGCTGGTCAGCCGCTTTCAGCGTGATGCGACGCAGAGCGGTGCCGTGCTGTAT
TTCGATATCGATGAATTCAAGACGATCAACGACACCTTCGGCCATCGGGTTGGCGACAACGTGCTGGTCCGGACGGCTGA
TGTCGTCAGCAAGTTGGTCCGTACTGGCGAGTTATTGGCCCGCCTGGGGGGGGACGAATTTGCCATTGTTGCGCCGGGGG
CAGATCTGGACGGCGTCCAGGCGATGGCCGAACGGGTCGTCAAAGCACTGACCGGCCTCAGTTTCGAGTTCGACGGCAAG
CGTTTCGGGCTGACCATCAGTCTCGGCATCGCGCTCTTGCCGGAGCACGCCGCCAGCGCCGAGGAACTGGTTGCCCACGC
CGATGCCGCAATGTACCAGGCCAAGCGCAGCGGCAAGAACTGCTGGCGCATCTATCGGCCCGACCTCGACGAATCCTCGC
ACATGCTCAGCCAGCTCGGCTGGAACGACAAGATCCAGGCGGCACTGAACGAAAACCGTCTGGTTTTGCACTTCCAGGGC
GTGCATGTAACGGCCACCCGAGCGATCAGCCACTACGAAGCCTTGGTTCGCATGATCGATCCCGACAACCCGGACGAATT
GCTGCAACCAGGCAGCTTCATTCCGGCCGCCGAGCGCACCGGGCGCATTGTCGACATCGACCGCTGGGTGATCGGCACCG
CCATTCACAAGCTGGCCGGCATGCCGAGTCTGCCTGCCCTCGCCATCAACGTGTCGGCCCGCTCCTTCGACGATCCGACG
CTGGCCCGCACGATCAGCGACCTGCTCACCGAGTTTCACGTCAACCCACAGCGCCTGATCATCGAACTGACCGAAACGGC
GGCGCTGGCCAACATCCGTGATTCAGAACGTTTCATCGACGACCTGCGCCGGCTCGGCTGCACTGTCTGCCTCGACGACT
TCGGCGTCGGCTTCTCGTCCTTCGCCTATCTGAAGCACCTGTCGGCCGATGTCCTGAAAATCGACGGCATGTTCATTCGC
AATCTGGTCAGCAATCGCGAGGATCAAGTCTTTGTCCGGGCCATTGTCGAAGTCGCCCGCGGCCTGGGCAAGAAGACCGT
TGCCGAATTCGTCGGTGATGCCCCGACACTGGCCCTGCTTGGCGAACTCGGCGTCGATTACGCGCAGGGTTACTACCTTT
CCTTCCCGATTGCCGAGATAAGCCCATCCTGA

Upstream 100 bases:

>100_bases
CCAAGCACGGCGGATTCCGCGAACCGACTGCCGAGGATTATGTGCTTCTCGACAAGATGATGCCGGAAACCCGTCGCCTG
TTGGGCAACACCGTCCGCTG

Downstream 100 bases:

>100_bases
TCGGGTAAAATGCTCCTTTTTTAGCCGCCGCAGGGAGTTCACCGTGCAAATCGTCTGTCTTGACCTCGAAGGGGTACTGG
TCCCCGAAATCTGGATCGAA

Product: diguanylate cyclase/phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 783; Mature: 782

Protein sequence:

>783_residues
MTLMKLPRSLRTRLITLSIIVEIVMISAIVWNSQRLIEAHLVRQFELRRAEISLLLQAALGAAMVQRDYAAVSETLTSAQ
QMQGMVYLIMFDERGQRIAAANAEHDIKPAGVRDFRVPVSVEKRPYGELQVGIDLRFLEEARQEILFQNIALALVGILTS
SVVLGGIALWLTRRLGRLVKASHDLADGRGFKPMPASSNDDIGQVISAFNGMAKTLDRRVRDLQEAEARQRALLETVDAE
RSRLDALLSAMRLGLVFVDQDERICYLNPAFASLWSVSLDGHLANLPLADLRRWLDAVLTNGGQSQLFRGDGTSVELALH
EGRVITQQGIAVTSASGSELGRLWIFEDVTNERQLAEHLLFMAERDPLTGLANRARFAAELDRLVSRFQRDATQSGAVLY
FDIDEFKTINDTFGHRVGDNVLVRTADVVSKLVRTGELLARLGGDEFAIVAPGADLDGVQAMAERVVKALTGLSFEFDGK
RFGLTISLGIALLPEHAASAEELVAHADAAMYQAKRSGKNCWRIYRPDLDESSHMLSQLGWNDKIQAALNENRLVLHFQG
VHVTATRAISHYEALVRMIDPDNPDELLQPGSFIPAAERTGRIVDIDRWVIGTAIHKLAGMPSLPALAINVSARSFDDPT
LARTISDLLTEFHVNPQRLIIELTETAALANIRDSERFIDDLRRLGCTVCLDDFGVGFSSFAYLKHLSADVLKIDGMFIR
NLVSNREDQVFVRAIVEVARGLGKKTVAEFVGDAPTLALLGELGVDYAQGYYLSFPIAEISPS

Sequences:

>Translated_783_residues
MTLMKLPRSLRTRLITLSIIVEIVMISAIVWNSQRLIEAHLVRQFELRRAEISLLLQAALGAAMVQRDYAAVSETLTSAQ
QMQGMVYLIMFDERGQRIAAANAEHDIKPAGVRDFRVPVSVEKRPYGELQVGIDLRFLEEARQEILFQNIALALVGILTS
SVVLGGIALWLTRRLGRLVKASHDLADGRGFKPMPASSNDDIGQVISAFNGMAKTLDRRVRDLQEAEARQRALLETVDAE
RSRLDALLSAMRLGLVFVDQDERICYLNPAFASLWSVSLDGHLANLPLADLRRWLDAVLTNGGQSQLFRGDGTSVELALH
EGRVITQQGIAVTSASGSELGRLWIFEDVTNERQLAEHLLFMAERDPLTGLANRARFAAELDRLVSRFQRDATQSGAVLY
FDIDEFKTINDTFGHRVGDNVLVRTADVVSKLVRTGELLARLGGDEFAIVAPGADLDGVQAMAERVVKALTGLSFEFDGK
RFGLTISLGIALLPEHAASAEELVAHADAAMYQAKRSGKNCWRIYRPDLDESSHMLSQLGWNDKIQAALNENRLVLHFQG
VHVTATRAISHYEALVRMIDPDNPDELLQPGSFIPAAERTGRIVDIDRWVIGTAIHKLAGMPSLPALAINVSARSFDDPT
LARTISDLLTEFHVNPQRLIIELTETAALANIRDSERFIDDLRRLGCTVCLDDFGVGFSSFAYLKHLSADVLKIDGMFIR
NLVSNREDQVFVRAIVEVARGLGKKTVAEFVGDAPTLALLGELGVDYAQGYYLSFPIAEISPS
>Mature_782_residues
TLMKLPRSLRTRLITLSIIVEIVMISAIVWNSQRLIEAHLVRQFELRRAEISLLLQAALGAAMVQRDYAAVSETLTSAQQ
MQGMVYLIMFDERGQRIAAANAEHDIKPAGVRDFRVPVSVEKRPYGELQVGIDLRFLEEARQEILFQNIALALVGILTSS
VVLGGIALWLTRRLGRLVKASHDLADGRGFKPMPASSNDDIGQVISAFNGMAKTLDRRVRDLQEAEARQRALLETVDAER
SRLDALLSAMRLGLVFVDQDERICYLNPAFASLWSVSLDGHLANLPLADLRRWLDAVLTNGGQSQLFRGDGTSVELALHE
GRVITQQGIAVTSASGSELGRLWIFEDVTNERQLAEHLLFMAERDPLTGLANRARFAAELDRLVSRFQRDATQSGAVLYF
DIDEFKTINDTFGHRVGDNVLVRTADVVSKLVRTGELLARLGGDEFAIVAPGADLDGVQAMAERVVKALTGLSFEFDGKR
FGLTISLGIALLPEHAASAEELVAHADAAMYQAKRSGKNCWRIYRPDLDESSHMLSQLGWNDKIQAALNENRLVLHFQGV
HVTATRAISHYEALVRMIDPDNPDELLQPGSFIPAAERTGRIVDIDRWVIGTAIHKLAGMPSLPALAINVSARSFDDPTL
ARTISDLLTEFHVNPQRLIIELTETAALANIRDSERFIDDLRRLGCTVCLDDFGVGFSSFAYLKHLSADVLKIDGMFIRN
LVSNREDQVFVRAIVEVARGLGKKTVAEFVGDAPTLALLGELGVDYAQGYYLSFPIAEISPS

Specific function: Probable signaling protein whose physiological role is not yet known [H]

COG id: COG5001

COG function: function code T; Predicted signal transduction protein containing a membrane domain, an EAL and a GGDEF domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1787541, Length=442, Percent_Identity=33.0316742081448, Blast_Score=227, Evalue=3e-60,
Organism=Escherichia coli, GI87081921, Length=427, Percent_Identity=28.5714285714286, Blast_Score=172, Evalue=9e-44,
Organism=Escherichia coli, GI1788849, Length=455, Percent_Identity=29.2307692307692, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI1788381, Length=564, Percent_Identity=25.177304964539, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI226510982, Length=428, Percent_Identity=26.8691588785047, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI87082096, Length=437, Percent_Identity=26.5446224256293, Blast_Score=125, Evalue=8e-30,
Organism=Escherichia coli, GI1790496, Length=234, Percent_Identity=32.9059829059829, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1789650, Length=415, Percent_Identity=26.7469879518072, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1787262, Length=162, Percent_Identity=38.2716049382716, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1788956, Length=164, Percent_Identity=43.2926829268293, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI87081743, Length=236, Percent_Identity=30.0847457627119, Blast_Score=103, Evalue=5e-23,
Organism=Escherichia coli, GI1786584, Length=174, Percent_Identity=36.2068965517241, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI87081881, Length=179, Percent_Identity=35.7541899441341, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1786507, Length=239, Percent_Identity=29.2887029288703, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1788085, Length=182, Percent_Identity=33.5164835164835, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI145693134, Length=165, Percent_Identity=33.9393939393939, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI87081845, Length=238, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI87081977, Length=171, Percent_Identity=31.5789473684211, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI87082007, Length=182, Percent_Identity=32.4175824175824, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1787802, Length=133, Percent_Identity=36.8421052631579, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI87081980, Length=200, Percent_Identity=32, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1787055, Length=243, Percent_Identity=28.3950617283951, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1788502, Length=238, Percent_Identity=26.4705882352941, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI1787056, Length=155, Percent_Identity=30.3225806451613, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1787816, Length=163, Percent_Identity=27.6073619631902, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI87081974, Length=112, Percent_Identity=34.8214285714286, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 86438; Mature: 86307

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS50885 HAMP ; PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLMKLPRSLRTRLITLSIIVEIVMISAIVWNSQRLIEAHLVRQFELRRAEISLLLQAAL
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAAMVQRDYAAVSETLTSAQQMQGMVYLIMFDERGQRIAAANAEHDIKPAGVRDFRVPVS
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCEEEECCCCCCCCCCCCCEEECCEE
VEKRPYGELQVGIDLRFLEEARQEILFQNIALALVGILTSSVVLGGIALWLTRRLGRLVK
ECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASHDLADGRGFKPMPASSNDDIGQVISAFNGMAKTLDRRVRDLQEAEARQRALLETVDAE
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSRLDALLSAMRLGLVFVDQDERICYLNPAFASLWSVSLDGHLANLPLADLRRWLDAVLT
HHHHHHHHHHHHHCEEEECCCCCEEEECHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHC
NGGQSQLFRGDGTSVELALHEGRVITQQGIAVTSASGSELGRLWIFEDVTNERQLAEHLL
CCCCCCEEECCCCEEEEEEECCEEEEECCEEEEECCCCCCCEEEEECCCCCHHHHHHHHH
FMAERDPLTGLANRARFAAELDRLVSRFQRDATQSGAVLYFDIDEFKTINDTFGHRVGDN
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHCCHHHHHHCCCCCCC
VLVRTADVVSKLVRTGELLARLGGDEFAIVAPGADLDGVQAMAERVVKALTGLSFEFDGK
CEEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEECCC
RFGLTISLGIALLPEHAASAEELVAHADAAMYQAKRSGKNCWRIYRPDLDESSHMLSQLG
EEEEEEEECHHCCCHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCC
WNDKIQAALNENRLVLHFQGVHVTATRAISHYEALVRMIDPDNPDELLQPGSFIPAAERT
CCCHHHHHCCCCEEEEEECCCEEEHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCHHHC
GRIVDIDRWVIGTAIHKLAGMPSLPALAINVSARSFDDPTLARTISDLLTEFHVNPQRLI
CCEEEEHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHEE
IELTETAALANIRDSERFIDDLRRLGCTVCLDDFGVGFSSFAYLKHLSADVLKIDGMFIR
EEEHHHHHHHCCCHHHHHHHHHHHCCCEEEEHHHCCCHHHHHHHHHCCHHHHHHHHHHHH
NLVSNREDQVFVRAIVEVARGLGKKTVAEFVGDAPTLALLGELGVDYAQGYYLSFPIAEI
HHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHCCHHHCCEEEEEEHHCC
SPS
CCC
>Mature Secondary Structure 
TLMKLPRSLRTRLITLSIIVEIVMISAIVWNSQRLIEAHLVRQFELRRAEISLLLQAAL
CHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAAMVQRDYAAVSETLTSAQQMQGMVYLIMFDERGQRIAAANAEHDIKPAGVRDFRVPVS
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCEEEECCCCCCCCCCCCCEEECCEE
VEKRPYGELQVGIDLRFLEEARQEILFQNIALALVGILTSSVVLGGIALWLTRRLGRLVK
ECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASHDLADGRGFKPMPASSNDDIGQVISAFNGMAKTLDRRVRDLQEAEARQRALLETVDAE
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSRLDALLSAMRLGLVFVDQDERICYLNPAFASLWSVSLDGHLANLPLADLRRWLDAVLT
HHHHHHHHHHHHHCEEEECCCCCEEEECHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHC
NGGQSQLFRGDGTSVELALHEGRVITQQGIAVTSASGSELGRLWIFEDVTNERQLAEHLL
CCCCCCEEECCCCEEEEEEECCEEEEECCEEEEECCCCCCCEEEEECCCCCHHHHHHHHH
FMAERDPLTGLANRARFAAELDRLVSRFQRDATQSGAVLYFDIDEFKTINDTFGHRVGDN
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHCCHHHHHHCCCCCCC
VLVRTADVVSKLVRTGELLARLGGDEFAIVAPGADLDGVQAMAERVVKALTGLSFEFDGK
CEEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEECCC
RFGLTISLGIALLPEHAASAEELVAHADAAMYQAKRSGKNCWRIYRPDLDESSHMLSQLG
EEEEEEEECHHCCCHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCC
WNDKIQAALNENRLVLHFQGVHVTATRAISHYEALVRMIDPDNPDELLQPGSFIPAAERT
CCCHHHHHCCCCEEEEEECCCEEEHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCHHHC
GRIVDIDRWVIGTAIHKLAGMPSLPALAINVSARSFDDPTLARTISDLLTEFHVNPQRLI
CCEEEEHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHEE
IELTETAALANIRDSERFIDDLRRLGCTVCLDDFGVGFSSFAYLKHLSADVLKIDGMFIR
EEEHHHHHHHCCCHHHHHHHHHHHCCCEEEEHHHCCCHHHHHHHHHCCHHHHHHHHHHHH
NLVSNREDQVFVRAIVEVARGLGKKTVAEFVGDAPTLALLGELGVDYAQGYYLSFPIAEI
HHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHCCHHHCCEEEEEEHHCC
SPS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11728710 [H]