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The map label for this gene is ydgK [H]

Identifier: 71905691

GI number: 71905691

Start: 58511

End: 59713

Strand: Reverse

Name: ydgK [H]

Synonym: Daro_0049

Alternate gene names: 71905691

Gene position: 59713-58511 (Counterclockwise)

Preceding gene: 71905692

Following gene: 71905689

Centisome position: 1.33

GC content: 64.17

Gene sequence:

>1203_bases
GTGGCTGAGGTCAGCCGGCGCGAGACGCGCGGCATCGCCATCCTGCTCGCCTCTATGTCGGCGATCGGGCCATTTTCGAT
CGACACCTACCTGCCGTCCTTCCACGAGATCGGCGAAAAACTGCATGCGACCCCGCTTGAAGTCCAGCAGACGCTGTCCA
TTTACCTGTTCTCCTTCGCGCTGATGACGTTATGGCACGGCGCGATTGCCGACCGTTTCGGACGCCGCAAGGTCATCCTG
ATCGCCCTTGGCTTGTTTGCCATCGCCTCGGCGGGTTGCACTCTCGCCACGCGCATCGAGCACCTGTGGTTCTGGCGGGC
CATGCAGGGCATCACGGCTGGCGCTGGCATCGTGCTCAGCCGCGCCATCGTGCGCGACCTGTTCGATGGCGCAGAAGCCC
AGCGCCTGATGTCACAGATCACCATGATGTTTGCGCTGGCCCCGGCCATCGCCCCGGTCATTGGCGGCTGGCTGCAAACG
CTGTTCGGCTGGCGTTCGGTCTTCGCCTTTCTCGTGCTGTCGACGACCGCATTGTGGCTGGCCTGCTGGAAACTGCTGCC
CGAAACCTTGCCGCCGGAAAAACGCCAGTCGCTGCGCCCCGCCTATCTCGCTTCAACCTACTGGAAAGTGCTGACCTCGC
CGCCTTTCCTGCTGGCTTGCGCCGCACTGTCGATGAATTTCGGCGGTTTCTTCCTGTACATCCTGTCCGCCCCGGTCTTC
CTGATGCGGCACCTCGGTGTGGCTGAAACCGGCTTCCTCTGGCTGTTCGGGCCGGCGATGAGTGGCCTGGTGTGCGGCTC
ATGGTTATCTGGCCGACTGGCCGGCAGGGTTTCGCTACCCCGCACCATCGGACTTGGCTATGCGGTCATGGGCACCGCAG
CCGCCATCAACCTGACGGTGAATGTTGCCTTGCCACCCGGCTTGCCATGGAGCGTGGCACCCATTTTCGTCTACACCATG
GGCATGTCGCTGGCCATGCCCTGCCTGACCATCTTCGCGCTCGACCCCTTCCCGGCCCAGCGCGGCCTGGCCGCATCGTG
CCAGACCTTCTTCCAGTCCAGCTTCAACGGTCTGGTTGCCGCGCTGATCGCCCCCGCCTTATGGGGCTCGACCCTGACAC
TGGCGCTCGGCATGGCCGGGCTGATGCTGACCGGTGGCCTCGCCGCCCTGCTCCACCAACGCCTAAGACTGGTACCACGG
TAG

Upstream 100 bases:

>100_bases
CGCATCCTGAAAGTCGCCCGAACCATTGCCGATCTGGCCGGCAACGACGACATCCGCGCCACCCACATCGCCGAGGCCAT
CCAGTACCGGCGCTTTGGCC

Downstream 100 bases:

>100_bases
TCCACGGCAGCGCCAGCCACCCAGCTTGCCGCGGTGATAGTCCTCGTCGAGCGGACCTTCGAAACCTTCCAGAACATTGA
TCACGTTGGTAAAACCGGCT

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MAEVSRRETRGIAILLASMSAIGPFSIDTYLPSFHEIGEKLHATPLEVQQTLSIYLFSFALMTLWHGAIADRFGRRKVIL
IALGLFAIASAGCTLATRIEHLWFWRAMQGITAGAGIVLSRAIVRDLFDGAEAQRLMSQITMMFALAPAIAPVIGGWLQT
LFGWRSVFAFLVLSTTALWLACWKLLPETLPPEKRQSLRPAYLASTYWKVLTSPPFLLACAALSMNFGGFFLYILSAPVF
LMRHLGVAETGFLWLFGPAMSGLVCGSWLSGRLAGRVSLPRTIGLGYAVMGTAAAINLTVNVALPPGLPWSVAPIFVYTM
GMSLAMPCLTIFALDPFPAQRGLAASCQTFFQSSFNGLVAALIAPALWGSTLTLALGMAGLMLTGGLAALLHQRLRLVPR

Sequences:

>Translated_400_residues
MAEVSRRETRGIAILLASMSAIGPFSIDTYLPSFHEIGEKLHATPLEVQQTLSIYLFSFALMTLWHGAIADRFGRRKVIL
IALGLFAIASAGCTLATRIEHLWFWRAMQGITAGAGIVLSRAIVRDLFDGAEAQRLMSQITMMFALAPAIAPVIGGWLQT
LFGWRSVFAFLVLSTTALWLACWKLLPETLPPEKRQSLRPAYLASTYWKVLTSPPFLLACAALSMNFGGFFLYILSAPVF
LMRHLGVAETGFLWLFGPAMSGLVCGSWLSGRLAGRVSLPRTIGLGYAVMGTAAAINLTVNVALPPGLPWSVAPIFVYTM
GMSLAMPCLTIFALDPFPAQRGLAASCQTFFQSSFNGLVAALIAPALWGSTLTLALGMAGLMLTGGLAALLHQRLRLVPR
>Mature_399_residues
AEVSRRETRGIAILLASMSAIGPFSIDTYLPSFHEIGEKLHATPLEVQQTLSIYLFSFALMTLWHGAIADRFGRRKVILI
ALGLFAIASAGCTLATRIEHLWFWRAMQGITAGAGIVLSRAIVRDLFDGAEAQRLMSQITMMFALAPAIAPVIGGWLQTL
FGWRSVFAFLVLSTTALWLACWKLLPETLPPEKRQSLRPAYLASTYWKVLTSPPFLLACAALSMNFGGFFLYILSAPVFL
MRHLGVAETGFLWLFGPAMSGLVCGSWLSGRLAGRVSLPRTIGLGYAVMGTAAAINLTVNVALPPGLPWSVAPIFVYTMG
MSLAMPCLTIFALDPFPAQRGLAASCQTFFQSSFNGLVAALIAPALWGSTLTLALGMAGLMLTGGLAALLHQRLRLVPR

Specific function: Involved In Sulfonamide (Sulfathiazole) And Bicyclomycin Resistance. Probable Membrane Translocase. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=372, Percent_Identity=27.4193548387097, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI48994889, Length=354, Percent_Identity=28.8135593220339, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790146, Length=323, Percent_Identity=27.8637770897833, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87082312, Length=343, Percent_Identity=24.7813411078717, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1790794, Length=314, Percent_Identity=22.2929936305732, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790195, Length=198, Percent_Identity=26.2626262626263, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=160, Percent_Identity=26.875, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43200; Mature: 43069

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS00093 N4_MTASE ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVSRRETRGIAILLASMSAIGPFSIDTYLPSFHEIGEKLHATPLEVQQTLSIYLFSFA
CCCCCHHHHCCHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHH
LMTLWHGAIADRFGRRKVILIALGLFAIASAGCTLATRIEHLWFWRAMQGITAGAGIVLS
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
RAIVRDLFDGAEAQRLMSQITMMFALAPAIAPVIGGWLQTLFGWRSVFAFLVLSTTALWL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ACWKLLPETLPPEKRQSLRPAYLASTYWKVLTSPPFLLACAALSMNFGGFFLYILSAPVF
HHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
LMRHLGVAETGFLWLFGPAMSGLVCGSWLSGRLAGRVSLPRTIGLGYAVMGTAAAINLTV
HHHHCCCCCCCCEEEECHHHHHHHHHHHHHCHHHCCCCCCHHHCCCHHHHHHHEEEEEEE
NVALPPGLPWSVAPIFVYTMGMSLAMPCLTIFALDPFPAQRGLAASCQTFFQSSFNGLVA
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ALIAPALWGSTLTLALGMAGLMLTGGLAALLHQRLRLVPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AEVSRRETRGIAILLASMSAIGPFSIDTYLPSFHEIGEKLHATPLEVQQTLSIYLFSFA
CCCCHHHHCCHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHH
LMTLWHGAIADRFGRRKVILIALGLFAIASAGCTLATRIEHLWFWRAMQGITAGAGIVLS
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
RAIVRDLFDGAEAQRLMSQITMMFALAPAIAPVIGGWLQTLFGWRSVFAFLVLSTTALWL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ACWKLLPETLPPEKRQSLRPAYLASTYWKVLTSPPFLLACAALSMNFGGFFLYILSAPVF
HHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
LMRHLGVAETGFLWLFGPAMSGLVCGSWLSGRLAGRVSLPRTIGLGYAVMGTAAAINLTV
HHHHCCCCCCCCEEEECHHHHHHHHHHHHHCHHHCCCCCCHHHCCCHHHHHHHEEEEEEE
NVALPPGLPWSVAPIFVYTMGMSLAMPCLTIFALDPFPAQRGLAASCQTFFQSSFNGLVA
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ALIAPALWGSTLTLALGMAGLMLTGGLAALLHQRLRLVPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9202461 [H]