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The map label for this gene is rpfG [H]

Identifier: 71905649

GI number: 71905649

Start: 7338

End: 8477

Strand: Reverse

Name: rpfG [H]

Synonym: Daro_0007

Alternate gene names: 71905649

Gene position: 8477-7338 (Counterclockwise)

Preceding gene: 71905650

Following gene: 71905648

Centisome position: 0.19

GC content: 58.07

Gene sequence:

>1140_bases
ATGTCCCCCCTCCTGCCCAAACAAAGCATCCTGGTCGTCGACGACAGCCCCGAGAATATCGACTTGCTGAGTGAAGTGCT
GCGCGACGAATATCGCATCCGCGTTGCCACCTCTGGCGAAAAGGCACTGAAAATTGTCTACTCGGATGAGCCGCCGGACC
TGATCCTGCTCGACATCATGATGCCGGGGCTCAGCGGACTGGAGATCTGCCGACGCCTGAAGGCCAATCCGGACCGCCGG
CGGATTCCGGTCATTTTCGTTACGGCCATGAGCAGCATGGATGACGAAAAGCGGGGTCTGGAAACCGGTGCCGTCGACTA
CATCACCAAGCCAATCAGCCCGCCCATCGTCAAGGCGCGCGTCCGCACCCACCTTGCCCTCTACGACCAGTCGCGCGAAC
TCGAACACATGGTGCAGCAACGCACCCACGAATTGCTGACCACTCGCCAACAAATCATCCGCCGCCTGGGTCGGGCGGCC
GAGTTCAAGGACAACGAAACCGGCAACCACGTCCTGCGCATGAGCCACTACGCCCGACTGATCGCGCATGCGCACGGCCT
GGGTGAAGAAGCCTCCAATGTCATTTTCAACACCGCCCCGATGCACGACATCGGCAAGATCGGTATTCCAGATTCCATCC
TGCTCAAGCCGGCCAAGCTGGATGCCGAGGAATGGAAAGTGATGTACCAGCACCCGATCATGGGGGCCGAAATCATCGGC
AAGCACGATAACGAACTGCTGGAAACCGCAAGGATCATCGCCCTGACCCATCACGAAAAATGGGATGGCACCGGCTACCC
GCAAGGCCTCAAGGGCGAGGCCATCCCGCTCGAAGGCCGCATCGTTGCCATTGCCGACGTCTTCGATGCCCTCGTTTCCG
TCCGCCCCTACAAAGCAGCCGTTCCACTCAATGAAGCGATGCAATATCTGGAATCCCAAAGCGGCCTTCATTTCGACCCC
AGCCTGATGGATGCTTTCCGAAAAGCCCTGCCTGAAATCCTCCGCATCAAGGAAATCTACGCCGACGAAAACGGCGCCCT
GACCGATCTGGAATTCCACATCAAGGAAATCTACGATCACAAGGACGATCCGCAAGGCTATCCGGAAAATCTGGCCAGTA
CGAATGGTGGTTTCGAGTAA

Upstream 100 bases:

>100_bases
AGATCAATCAGTATGAATTCGATGTAGCCATGGAAACATTGCAGCAAATGACCATCAACCGGCAACAAGTCTGAGACACC
TAATCGAGAGTCGCCAAGCC

Downstream 100 bases:

>100_bases
GCCGGCTCGTCCTGAAACTCACCCACGGGGAAAACTGATCTCCCAATTGGACACGACAGCCCTCCCGGCGACGGAAATAG
CCATTTGTCGATGACCGATC

Product: response regulator receiver modulated metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSPLLPKQSILVVDDSPENIDLLSEVLRDEYRIRVATSGEKALKIVYSDEPPDLILLDIMMPGLSGLEICRRLKANPDRR
RIPVIFVTAMSSMDDEKRGLETGAVDYITKPISPPIVKARVRTHLALYDQSRELEHMVQQRTHELLTTRQQIIRRLGRAA
EFKDNETGNHVLRMSHYARLIAHAHGLGEEASNVIFNTAPMHDIGKIGIPDSILLKPAKLDAEEWKVMYQHPIMGAEIIG
KHDNELLETARIIALTHHEKWDGTGYPQGLKGEAIPLEGRIVAIADVFDALVSVRPYKAAVPLNEAMQYLESQSGLHFDP
SLMDAFRKALPEILRIKEIYADENGALTDLEFHIKEIYDHKDDPQGYPENLASTNGGFE

Sequences:

>Translated_379_residues
MSPLLPKQSILVVDDSPENIDLLSEVLRDEYRIRVATSGEKALKIVYSDEPPDLILLDIMMPGLSGLEICRRLKANPDRR
RIPVIFVTAMSSMDDEKRGLETGAVDYITKPISPPIVKARVRTHLALYDQSRELEHMVQQRTHELLTTRQQIIRRLGRAA
EFKDNETGNHVLRMSHYARLIAHAHGLGEEASNVIFNTAPMHDIGKIGIPDSILLKPAKLDAEEWKVMYQHPIMGAEIIG
KHDNELLETARIIALTHHEKWDGTGYPQGLKGEAIPLEGRIVAIADVFDALVSVRPYKAAVPLNEAMQYLESQSGLHFDP
SLMDAFRKALPEILRIKEIYADENGALTDLEFHIKEIYDHKDDPQGYPENLASTNGGFE
>Mature_378_residues
SPLLPKQSILVVDDSPENIDLLSEVLRDEYRIRVATSGEKALKIVYSDEPPDLILLDIMMPGLSGLEICRRLKANPDRRR
IPVIFVTAMSSMDDEKRGLETGAVDYITKPISPPIVKARVRTHLALYDQSRELEHMVQQRTHELLTTRQQIIRRLGRAAE
FKDNETGNHVLRMSHYARLIAHAHGLGEEASNVIFNTAPMHDIGKIGIPDSILLKPAKLDAEEWKVMYQHPIMGAEIIGK
HDNELLETARIIALTHHEKWDGTGYPQGLKGEAIPLEGRIVAIADVFDALVSVRPYKAAVPLNEAMQYLESQSGLHFDPS
LMDAFRKALPEILRIKEIYADENGALTDLEFHIKEIYDHKDDPQGYPENLASTNGGFE

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=128, Percent_Identity=43.75, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1786599, Length=75, Percent_Identity=53.3333333333333, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87082012, Length=117, Percent_Identity=36.7521367521368, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1790860, Length=125, Percent_Identity=40, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1789809, Length=128, Percent_Identity=39.84375, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1788550, Length=212, Percent_Identity=26.4150943396226, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790437, Length=119, Percent_Identity=36.9747899159664, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1789149, Length=105, Percent_Identity=34.2857142857143, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI145693140, Length=110, Percent_Identity=34.5454545454545, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787229, Length=141, Percent_Identity=31.2056737588652, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1786784, Length=117, Percent_Identity=31.6239316239316, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 42655; Mature: 42523

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPLLPKQSILVVDDSPENIDLLSEVLRDEYRIRVATSGEKALKIVYSDEPPDLILLDIM
CCCCCCCCCEEEEECCCCCHHHHHHHHHCCEEEEEEECCCEEEEEEECCCCCCEEEEEEH
MPGLSGLEICRRLKANPDRRRIPVIFVTAMSSMDDEKRGLETGAVDYITKPISPPIVKAR
HCCCCHHHHHHHHHCCCCCCCCCEEEEEEHHCCCHHHHCCCCCCHHHHHCCCCCHHHHHH
VRTHLALYDQSRELEHMVQQRTHELLTTRQQIIRRLGRAAEFKDNETGNHVLRMSHYARL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHH
IAHAHGLGEEASNVIFNTAPMHDIGKIGIPDSILLKPAKLDAEEWKVMYQHPIMGAEIIG
HHHHHCCCCHHCCCEEECCCCCCCCCCCCCHHHEECCCCCCHHHHHHHHHCCCCCHHHHC
KHDNELLETARIIALTHHEKWDGTGYPQGLKGEAIPLEGRIVAIADVFDALVSVRPYKAA
CCCHHHHHHHHHEEEEECCCCCCCCCCCCCCCCEECCCCCEEHHHHHHHHHHCCCCCCCC
VPLNEAMQYLESQSGLHFDPSLMDAFRKALPEILRIKEIYADENGALTDLEFHIKEIYDH
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCC
KDDPQGYPENLASTNGGFE
CCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure 
SPLLPKQSILVVDDSPENIDLLSEVLRDEYRIRVATSGEKALKIVYSDEPPDLILLDIM
CCCCCCCCEEEEECCCCCHHHHHHHHHCCEEEEEEECCCEEEEEEECCCCCCEEEEEEH
MPGLSGLEICRRLKANPDRRRIPVIFVTAMSSMDDEKRGLETGAVDYITKPISPPIVKAR
HCCCCHHHHHHHHHCCCCCCCCCEEEEEEHHCCCHHHHCCCCCCHHHHHCCCCCHHHHHH
VRTHLALYDQSRELEHMVQQRTHELLTTRQQIIRRLGRAAEFKDNETGNHVLRMSHYARL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHH
IAHAHGLGEEASNVIFNTAPMHDIGKIGIPDSILLKPAKLDAEEWKVMYQHPIMGAEIIG
HHHHHCCCCHHCCCEEECCCCCCCCCCCCCHHHEECCCCCCHHHHHHHHHCCCCCHHHHC
KHDNELLETARIIALTHHEKWDGTGYPQGLKGEAIPLEGRIVAIADVFDALVSVRPYKAA
CCCHHHHHHHHHEEEEECCCCCCCCCCCCCCCCEECCCCCEEHHHHHHHHHHCCCCCCCC
VPLNEAMQYLESQSGLHFDPSLMDAFRKALPEILRIKEIYADENGALTDLEFHIKEIYDH
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCC
KDDPQGYPENLASTNGGFE
CCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]