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The map label for this gene is yfkN [H]

Identifier: 71893979

GI number: 71893979

Start: 829353

End: 831089

Strand: Reverse

Name: yfkN [H]

Synonym: MHJ_0631

Alternate gene names: 71893979

Gene position: 831089-829353 (Counterclockwise)

Preceding gene: 71893984

Following gene: 71893978

Centisome position: 92.61

GC content: 33.97

Gene sequence:

>1737_bases
ATTTTTCATACAAACGACGAACACGGTCGCTTAGAATATGATGATAACAAACACAATAATTATTCAGGGATGGATAAAAC
GGGACAGTACTTGAAAAAATTTAACAAGGATTTACTTTTATCTGCCGGAGATTTAATTCAAGGACTTCCACTTTCGGATA
CAGATAAAGGTAAGACAATTGCAAAAATTGCCAAATATATAGGCTATGATTCAGTTGCGATTGGAAACCATGAATTCGAC
TATGGAATTAGACACATTCTTGATCTTGCCGCCGAGGGAAACAAAGATGAATTTAGCCGAAAAATGCCTTTTATTTCCGC
TAATGTTTACTGAAGACAACCTTCAGAAGAGGAACTTAAAAATTTAGAATCTACAGATGAAAGAGCCATAAAACCAGCTG
AATTTAAACAAGGGAAGCGAGTTTTCCAACCTTATATAATAAAGGAATTAGAAAATGGTCTAAAAGTTGCAGTTATCGGG
CTGACAACTCCAGATACAAAAATTACTTCCCACCCAAAAAATTCTTTTTGAGTTGAATTTACCGATCCAGTACCTGAAAC
CCAAAAGGTAATTAAGGAGATTCAGGAAAAAGATCCTTCAATTTCCTTTATAATTGCTACAACGCACCTAGGTGTTGGGC
GCACAGAACATAAATGAACTTCAGATTATCTTGCAGAACAAACCGGAAATGGCCTTGATTTAGTCATCGATGGGCATTCG
CATACAAAAATCGAAATTCACAAACCCAAAGAAGATAAAAAAGTTTGGGTAACCCAAACTGAGGCCTATGCAAAATGGCT
CGGGGACATTGATTTAGTTTTTGATACTGAAACTGGCGAGATAGTAAAAATTGTACAATCTTTAAGAGATATTAACCAAA
TCAATATTGTAACCAGAGATTTATCAGAACATTATATCAAAAAACTTCATAAAGTTTATGATGTAGAAAATGATGTAAAA
GTATTTAATTCGCCTGGTGTTTTTGAACATGTTCAATCAATTGAGATCAACAAAACTCCTTACTGAATTGGACGGGTAAA
ACCAACTTCATTAGGGGTAATGACAGCAGATGCCATTGCTTGGGAATATGCAAAAAGTTCAAAAGAACAAGTACAAAGTA
CGAAAAATGAAATTGCAACACTTGATAATTCCTTAGGACTTATAAACGGTGGTAGTCTTAGAACAGATCTAAAAAGTGGT
GAAATCAAAAGAGGAGATGTTTTAGGGGTTAGTCCTTTTGGGAATAGGATTGTAACTATTAAACTAAAAGGAGATACACT
TAAAAAAACTCTCGAATACGGACTTTCTATGGGGAAACAAGGCGCATTTGCCCAACTATCTTCAAATATTTCCTATAAAG
TCAAAGTTGAAAAAGGGACTGATCCGAAAACCAAAATAGAATCCTGGGTTTGAAAACCAGATACAACATCCTTTAAAATT
AATAACAAGCCGATTGATGATAACAAATTTTACTATTTAAGCACAAATGATTATCTTTCAGCGGGCGGGGACGGCTATCA
AATGTTAAATCTAGGTAAAAACAGGGATATTGAAAAAGTTTATGAGGGAGTTCAATATATTGATTCTTTAATTAAATATG
GTCAATATTTGGACAAATTAACTAAAGATTCAAGTCAAAAAGATCTATTTGCCCACACATTCCAAGAATATTTAAGTTCT
GATTTTACAAAAAATCAACAAGTTGAGATTCCTCAAGAAGCTCTAACGAAAAGCTAA

Upstream 100 bases:

>100_bases
CCAAGCCAAAATTGATCCCCTAATTGATCAGGTCAATTATCTTTTTAAAAAATTGCATGAATTAGAAAAAAAAGAAAATT
CAAAACTTAAAACTGTAAAA

Downstream 100 bases:

>100_bases
AGTCAATCTTAATTATTTTAATTTTTTTAAAAAGACACACTTTTGAGTGTGTTTTTTTATTTTTTTACCTTTTTTTGCAA
AAAAAAAAAAAAAAAAAGGT

Product: putative 5'-nucleotidase precursor

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MFHTNDEHGRLEYDDNKHNNYSGMDKTGQYLKKFNKDLLLSAGDLIQGLPLSDTDKGKTIAKIAKYIGYDSVAIGNHEFD
YGIRHILDLAAEGNKDEFSRKMPFISANVYWRQPSEEELKNLESTDERAIKPAEFKQGKRVFQPYIIKELENGLKVAVIG
LTTPDTKITSHPKNSFWVEFTDPVPETQKVIKEIQEKDPSISFIIATTHLGVGRTEHKWTSDYLAEQTGNGLDLVIDGHS
HTKIEIHKPKEDKKVWVTQTEAYAKWLGDIDLVFDTETGEIVKIVQSLRDINQINIVTRDLSEHYIKKLHKVYDVENDVK
VFNSPGVFEHVQSIEINKTPYWIGRVKPTSLGVMTADAIAWEYAKSSKEQVQSTKNEIATLDNSLGLINGGSLRTDLKSG
EIKRGDVLGVSPFGNRIVTIKLKGDTLKKTLEYGLSMGKQGAFAQLSSNISYKVKVEKGTDPKTKIESWVWKPDTTSFKI
NNKPIDDNKFYYLSTNDYLSAGGDGYQMLNLGKNRDIEKVYEGVQYIDSLIKYGQYLDKLTKDSSQKDLFAHTFQEYLSS
DFTKNQQVEIPQEALTKS

Sequences:

>Translated_578_residues
MFHTNDEHGRLEYDDNKHNNYSGMDKTGQYLKKFNKDLLLSAGDLIQGLPLSDTDKGKTIAKIAKYIGYDSVAIGNHEFD
YGIRHILDLAAEGNKDEFSRKMPFISANVY*RQPSEEELKNLESTDERAIKPAEFKQGKRVFQPYIIKELENGLKVAVIG
LTTPDTKITSHPKNSF*VEFTDPVPETQKVIKEIQEKDPSISFIIATTHLGVGRTEHK*TSDYLAEQTGNGLDLVIDGHS
HTKIEIHKPKEDKKVWVTQTEAYAKWLGDIDLVFDTETGEIVKIVQSLRDINQINIVTRDLSEHYIKKLHKVYDVENDVK
VFNSPGVFEHVQSIEINKTPY*IGRVKPTSLGVMTADAIAWEYAKSSKEQVQSTKNEIATLDNSLGLINGGSLRTDLKSG
EIKRGDVLGVSPFGNRIVTIKLKGDTLKKTLEYGLSMGKQGAFAQLSSNISYKVKVEKGTDPKTKIESWV*KPDTTSFKI
NNKPIDDNKFYYLSTNDYLSAGGDGYQMLNLGKNRDIEKVYEGVQYIDSLIKYGQYLDKLTKDSSQKDLFAHTFQEYLSS
DFTKNQQVEIPQEALTKS
>Mature_578_residues
MFHTNDEHGRLEYDDNKHNNYSGMDKTGQYLKKFNKDLLLSAGDLIQGLPLSDTDKGKTIAKIAKYIGYDSVAIGNHEFD
YGIRHILDLAAEGNKDEFSRKMPFISANVY*RQPSEEELKNLESTDERAIKPAEFKQGKRVFQPYIIKELENGLKVAVIG
LTTPDTKITSHPKNSF*VEFTDPVPETQKVIKEIQEKDPSISFIIATTHLGVGRTEHK*TSDYLAEQTGNGLDLVIDGHS
HTKIEIHKPKEDKKVWVTQTEAYAKWLGDIDLVFDTETGEIVKIVQSLRDINQINIVTRDLSEHYIKKLHKVYDVENDVK
VFNSPGVFEHVQSIEINKTPY*IGRVKPTSLGVMTADAIAWEYAKSSKEQVQSTKNEIATLDNSLGLINGGSLRTDLKSG
EIKRGDVLGVSPFGNRIVTIKLKGDTLKKTLEYGLSMGKQGAFAQLSSNISYKVKVEKGTDPKTKIESWV*KPDTTSFKI
NNKPIDDNKFYYLSTNDYLSAGGDGYQMLNLGKNRDIEKVYEGVQYIDSLIKYGQYLDKLTKDSSQKDLFAHTFQEYLSS
DFTKNQQVEIPQEALTKS

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=555, Percent_Identity=24.8648648648649, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786687, Length=547, Percent_Identity=25.7769652650823, Blast_Score=140, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19922444, Length=556, Percent_Identity=24.6402877697842, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI28573524, Length=550, Percent_Identity=23.8181818181818, Blast_Score=102, Evalue=9e-22,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 64500; Mature: 64500

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHTNDEHGRLEYDDNKHNNYSGMDKTGQYLKKFNKDLLLSAGDLIQGLPLSDTDKGKTI
CCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHCHHHEEECCHHHCCCCCCCCCCCHHH
AKIAKYIGYDSVAIGNHEFDYGIRHILDLAAEGNKDEFSRKMPFISANVYRQPSEEELKN
HHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHCCCEEECCCCCCCCHHHHHC
LESTDERAIKPAEFKQGKRVFQPYIIKELENGLKVAVIGLTTPDTKITSHPKNSFVEFTD
CCCCHHHCCCCHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEECC
PVPETQKVIKEIQEKDPSISFIIATTHLGVGRTEHKTSDYLAEQTGNGLDLVIDGHSHTK
CCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCE
IEIHKPKEDKKVWVTQTEAYAKWLGDIDLVFDTETGEIVKIVQSLRDINQINIVTRDLSE
EEEECCCCCCEEEEEEHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCHHEEEHHHHHH
HYIKKLHKVYDVENDVKVFNSPGVFEHVQSIEINKTPYIGRVKPTSLGVMTADAIAWEYA
HHHHHHHHHHCCCCCCEEECCCCHHHHHHHEECCCCCCCCCCCCCCCCEEEHHHHHHHHH
KSSKEQVQSTKNEIATLDNSLGLINGGSLRTDLKSGEIKRGDVLGVSPFGNRIVTIKLKG
HHHHHHHHHHHHHHHHHCCCCCEEECCCEEECCCCCCCCCCCEEEECCCCCEEEEEEECC
DTLKKTLEYGLSMGKQGAFAQLSSNISYKVKVEKGTDPKTKIESWVKPDTTSFKINNKPI
HHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEECCCCCHHHHHHHCCCCCCEEEECCCCC
DDNKFYYLSTNDYLSAGGDGYQMLNLGKNRDIEKVYEGVQYIDSLIKYGQYLDKLTKDSS
CCCCEEEEECCCEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QKDLFAHTFQEYLSSDFTKNQQVEIPQEALTKS
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCC
>Mature Secondary Structure
MFHTNDEHGRLEYDDNKHNNYSGMDKTGQYLKKFNKDLLLSAGDLIQGLPLSDTDKGKTI
CCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHCHHHEEECCHHHCCCCCCCCCCCHHH
AKIAKYIGYDSVAIGNHEFDYGIRHILDLAAEGNKDEFSRKMPFISANVYRQPSEEELKN
HHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHCCCEEECCCCCCCCHHHHHC
LESTDERAIKPAEFKQGKRVFQPYIIKELENGLKVAVIGLTTPDTKITSHPKNSFVEFTD
CCCCHHHCCCCHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEECC
PVPETQKVIKEIQEKDPSISFIIATTHLGVGRTEHKTSDYLAEQTGNGLDLVIDGHSHTK
CCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCE
IEIHKPKEDKKVWVTQTEAYAKWLGDIDLVFDTETGEIVKIVQSLRDINQINIVTRDLSE
EEEECCCCCCEEEEEEHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCHHEEEHHHHHH
HYIKKLHKVYDVENDVKVFNSPGVFEHVQSIEINKTPYIGRVKPTSLGVMTADAIAWEYA
HHHHHHHHHHCCCCCCEEECCCCHHHHHHHEECCCCCCCCCCCCCCCCEEEHHHHHHHHH
KSSKEQVQSTKNEIATLDNSLGLINGGSLRTDLKSGEIKRGDVLGVSPFGNRIVTIKLKG
HHHHHHHHHHHHHHHHHCCCCCEEECCCEEECCCCCCCCCCCEEEECCCCCEEEEEEECC
DTLKKTLEYGLSMGKQGAFAQLSSNISYKVKVEKGTDPKTKIESWVKPDTTSFKINNKPI
HHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEECCCCCHHHHHHHCCCCCCEEEECCCCC
DDNKFYYLSTNDYLSAGGDGYQMLNLGKNRDIEKVYEGVQYIDSLIKYGQYLDKLTKDSS
CCCCEEEEECCCEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QKDLFAHTFQEYLSSDFTKNQQVEIPQEALTKS
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]