Click here to switch to the map view.

The map label for this gene is ddem [H]

Identifier: 71893964

GI number: 71893964

Start: 798962

End: 799951

Strand: Reverse

Name: ddem [H]

Synonym: MHJ_0615

Alternate gene names: 71893964

Gene position: 799951-798962 (Counterclockwise)

Preceding gene: 71893965

Following gene: 71893963

Centisome position: 89.14

GC content: 34.65

Gene sequence:

>990_bases
ATGAAGAAACTTGATGGCTGGAGCTCAAGTTATCACAATGGTTATAATTTTATCGATCTTTTTTCGGGTGCTGGCGGCCT
TTCTTGTGGTCTTGTTATGGCGGGATTTTTGCCCTTGGCAAGTTTAGAAATAATGAAACAAGCCGTTGAAACCTATGCCT
ATAATTTTAAAAAAAGGTCAAAAAATAAAGAACTTTTTAAGCTAAGCGATATTAGAGATTCTAAAATAAAATCCGAATTT
TATGATCATTTCAAGGATCAAGAACTAGATTTAATTGCTGGAGGTTTTCCTTGTCAGGGCTTTTCGATGGCAGGTAATCG
CGTCTTTGATGATCCCCGAAATAATTTATACCTTGAAATGTTAGATATTGTTGCGAATTTAAAGCCAAAATTCGTGTTAA
TGGAGAATGTTCAAGGACTTAGAACCATGCTTTCTGGTCAAATTGAGGTCAAAATCATAAAAGATTTTGAAAAAATCGGC
TATAAGATTAATGTAGCAACATTAAATAGTGCCGATTTTGGAGTCGCTCAAATAAGAAAACGGGTAATTTTTATTGCAAA
CCGGATTGGTTTGACAAATTTTTTCCCTGCAGCTATTCTTAGCCCAGAAAATTATAAAACTTTAGGTCAGTGTATTGAAA
AATATATGAATTGAGAAGAAAATATTGAAATTAATCATATTTTTACAAAACATTCAGCTAAAATGAGGGAAAAAATTTTA
AAGACATCCGAAGGCCAAAGCGTTTATAAAAATTACTCTGATGGTTGGAAGAAATCACCTTGAGATCAGCCTAGTTGTAC
AGTTAAAAAAAATCATGGCGGGGTGAATCTCCATCCTAAACTTCCAAGGGTTTTAACTCCGCGTGAGTTGGCTGACTTAC
AGTCATTTCCTGATAGTTTTATTTTTAAAGGGTCAAAAAAAATGACAACTTGTGCAAATTGGAAATGCGGTGCCCCCACT
TTTAGCAAAGGCTATCGGCCTGGCTATTAA

Upstream 100 bases:

>100_bases
AATGAAAATCATCCTATAGAAAACAAGAAAAACTAAGGAAAAATTTAGGAAAAACTAAAGAAAAAGTCCACTTTATTGAG
ATCTCAGAAGAAATGAAGAA

Downstream 100 bases:

>100_bases
AAAATCTCTGGATAAGAAAGATGTATTATAAGGTTTTAAATTTTTCCAGAAGCAAATTGAGGATTATACCAAACAAATCT
TTAAGGTTTATGGAAATGAA

Product: cytosine specific DNA methyltransferase

Products: NA

Alternate protein names: M.HphIA; Cytosine-specific methyltransferase HphIA; M.Hphi(C) [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKKLDGWSSSYHNGYNFIDLFSGAGGLSCGLVMAGFLPLASLEIMKQAVETYAYNFKKRSKNKELFKLSDIRDSKIKSEF
YDHFKDQELDLIAGGFPCQGFSMAGNRVFDDPRNNLYLEMLDIVANLKPKFVLMENVQGLRTMLSGQIEVKIIKDFEKIG
YKINVATLNSADFGVAQIRKRVIFIANRIGLTNFFPAAILSPENYKTLGQCIEKYMNWEENIEINHIFTKHSAKMREKIL
KTSEGQSVYKNYSDGWKKSPWDQPSCTVKKNHGGVNLHPKLPRVLTPRELADLQSFPDSFIFKGSKKMTTCANWKCGAPT
FSKGYRPGY

Sequences:

>Translated_329_residues
MKKLDGWSSSYHNGYNFIDLFSGAGGLSCGLVMAGFLPLASLEIMKQAVETYAYNFKKRSKNKELFKLSDIRDSKIKSEF
YDHFKDQELDLIAGGFPCQGFSMAGNRVFDDPRNNLYLEMLDIVANLKPKFVLMENVQGLRTMLSGQIEVKIIKDFEKIG
YKINVATLNSADFGVAQIRKRVIFIANRIGLTNFFPAAILSPENYKTLGQCIEKYMN*EENIEINHIFTKHSAKMREKIL
KTSEGQSVYKNYSDGWKKSP*DQPSCTVKKNHGGVNLHPKLPRVLTPRELADLQSFPDSFIFKGSKKMTTCANWKCGAPT
FSKGYRPGY
>Mature_329_residues
MKKLDGWSSSYHNGYNFIDLFSGAGGLSCGLVMAGFLPLASLEIMKQAVETYAYNFKKRSKNKELFKLSDIRDSKIKSEF
YDHFKDQELDLIAGGFPCQGFSMAGNRVFDDPRNNLYLEMLDIVANLKPKFVLMENVQGLRTMLSGQIEVKIIKDFEKIG
YKINVATLNSADFGVAQIRKRVIFIANRIGLTNFFPAAILSPENYKTLGQCIEKYMN*EENIEINHIFTKHSAKMREKIL
KTSEGQSVYKNYSDGWKKSP*DQPSCTVKKNHGGVNLHPKLPRVLTPRELADLQSFPDSFIFKGSKKMTTCANWKCGAPT
FSKGYRPGY

Specific function: This methylase protects the DNA from cleavage by the HphI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4503351, Length=181, Percent_Identity=27.6243093922652, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI195927037, Length=181, Percent_Identity=27.6243093922652, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1788271, Length=337, Percent_Identity=24.9258160237389, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 36995; Mature: 36995

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLDGWSSSYHNGYNFIDLFSGAGGLSCGLVMAGFLPLASLEIMKQAVETYAYNFKKRS
CCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KNKELFKLSDIRDSKIKSEFYDHFKDQELDLIAGGFPCQGFSMAGNRVFDDPRNNLYLEM
CCCCEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHH
LDIVANLKPKFVLMENVQGLRTMLSGQIEVKIIKDFEKIGYKINVATLNSADFGVAQIRK
HHHHHCCCCCEEEHHHHHHHHHHHCCCEEEEEEHHHHHCCEEEEEEEECCCCCCHHHHHH
RVIFIANRIGLTNFFPAAILSPENYKTLGQCIEKYMNEENIEINHIFTKHSAKMREKILK
HHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHH
TSEGQSVYKNYSDGWKKSPDQPSCTVKKNHGGVNLHPKLPRVLTPRELADLQSFPDSFIF
CCCCHHHHHCCCCCCCCCCCCCCCEEEECCCCEEECCCCCCCCCCHHHHHHHHCCHHHEE
KGSKKMTTCANWKCGAPTFSKGYRPGY
CCCCCCEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKLDGWSSSYHNGYNFIDLFSGAGGLSCGLVMAGFLPLASLEIMKQAVETYAYNFKKRS
CCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KNKELFKLSDIRDSKIKSEFYDHFKDQELDLIAGGFPCQGFSMAGNRVFDDPRNNLYLEM
CCCCEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHH
LDIVANLKPKFVLMENVQGLRTMLSGQIEVKIIKDFEKIGYKINVATLNSADFGVAQIRK
HHHHHCCCCCEEEHHHHHHHHHHHCCCEEEEEEHHHHHCCEEEEEEEECCCCCCHHHHHH
RVIFIANRIGLTNFFPAAILSPENYKTLGQCIEKYMNEENIEINHIFTKHSAKMREKILK
HHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHH
TSEGQSVYKNYSDGWKKSPDQPSCTVKKNHGGVNLHPKLPRVLTPRELADLQSFPDSFIF
CCCCHHHHHCCCCCCCCCCCCCCCEEEECCCCEEECCCCCCCCCCHHHHHHHHCCHHHEE
KGSKKMTTCANWKCGAPTFSKGYRPGY
CCCCCCEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759008 [H]