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The map label for this gene is thrS [H]

Identifier: 71893938

GI number: 71893938

Start: 762018

End: 763757

Strand: Reverse

Name: thrS [H]

Synonym: MHJ_0589

Alternate gene names: 71893938

Gene position: 763757-762018 (Counterclockwise)

Preceding gene: 71893939

Following gene: 71893937

Centisome position: 85.11

GC content: 29.6

Gene sequence:

>1740_bases
ATGGACTTAAAAATTGACTATAAATTAAATCATTCAAGTTCACACCTCTTAGCATTAGCAATTAAAATTCTTTATCCGGG
TGTAAAATTTGGAATTGGCCCTGTTTTTGATGATGGTTTTTATTATGATTTTGATCTTTCAGTCTCAATTTCGCAATCTG
ATTTTCCAAAAATTGAGAAATTAATGAAAAAGTTGGTTTCAAAAAATCTAAAGATTGTTGAAACCGATGGGAAGAACCTT
GATTTTCAAAATCAAATTTATAAAGCGGAATTAAAAAATGACCTAGAAAAAAAAGGTGAAAAAATCACTTATTTTTCAAT
AGTTGATAGTAAAAACAACATACTTTTTGAAGACTTATGTTATGGAAATCATCTTGAATCTCTAGGAAAAATTCAAAATT
TTAAACTTTTAAAAATTGCCGGCGCTTATTGAAAAGGTGATTCTAAAAACAAACAGTTAACCCGAATTTATGGAACTAGT
TGAGATTCAAAAGAAAATCTTGATAAATTTCTTGGAATTTTACAAGAAAGACAAGAAAGAGATCATCGAAAAATCGGGGC
AAAATTAAAACTGTTTACATTTAGTTCTTATTTTGGCCATGGTTTTCCAGTTTGACTTGAAAATGGAATGCGAATTTATA
ATAAAATTCGCCAAAAAATTCTTGAATTTGACCGTAATTACGGATTTAAAGAGGTTCTTACGCCCCATTTTGGCCATAAG
GAATTATATAAAATTTCCGGACATTTATTACATTATCGCGATGATATGTTTAAAGGCTTAAAAATTGAAAACGAGGAGCT
AATTCCGCGTCCGATGACTTGCCCACATCATATTATCCTTTTTTCACAGCAACTTTTTAGTTATCGAAATTTACCATATC
GAATCTCTGAACAATCACGATTATATCGTTATGAAAAATCAGGAGCACTTTTAGGCCTTGAACGCGTTCGAGCTATGGAT
TTAACAGAAGGGCATATTTTTGTTAGTAAAAGTCAAATTTTTAGTGAAATTAGTCATCTTTTTCAAATGATTTCTGAGGT
ACTTGATTTTTTTAAGGTCCAAATTTTTTATATCTCATTTTCAAAAAGAGACCCTAAAAACAAGGATAAATTTTTTCAAG
ATGATCAAATGTGGGAACAGGCAGAAGCTGATTTAAAGCAGTTTTTAGATCAAAATAAAATTAATTATATAGAAAAAATC
GGTGAAGCTGCTTTTTATGGTCCCAAAATTGACTTCCAAGTTAAAACTGCACTAAATAAAGAGTTAACAATTTCAACTTT
ACAACTTGATTTCCTTTTACCTGAAAAATTTAAAATTTCCTTTATAAATGAGTATAATAAAAAAGAAACTCCAATTTTAA
TTCACCGCGGTTTAATAGGCACTTATGAACGGTTTATAGCGCTTTTATTAGAACAAAGCAAGGGAAATTTACCTTTCTGA
ATCTCGCCAAAACAGATAATTGTTATTCCAGTAGCTGAAAAACATAATGATTTTGCCCAAAAAATTCATAAAATTCTTTT
TGATCTTGGATTTAATTCGGAAATTGATCTCAGATCTGAGCGCATTTCGCGCAAAATCCGCGAGGCAAATTTAGAAAAAA
TAAATTTTCAGGTAATAATCGGGGATGAGGAAATTACAAAAAATGAAATTACCTATAGAAAATTTGGTTCTCCTGAATTA
TTTAGAATTTCCCTAGTTGATTTTGTCGATTTGTTGAAAAACAGCGAAAAAAATGTATAA

Upstream 100 bases:

>100_bases
CGATTTAGTCGAGATTTATGCAAAAAAAGGCGCTAAAAAAGCAAAAGAAGTTGCTGTTTCTAACTTTGAAAAAATCAAAG
AAAAAATAGGATTATAATTT

Downstream 100 bases:

>100_bases
AAAAATAACGAAAATTCCTGATTTTTTATTTGCATTGCTTACAATAATTCTTCCGATTAGCCTTATTTATGCATTTTTTT
CGCCTGATTTTTATGGAAAA

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MDLKIDYKLNHSSSHLLALAIKILYPGVKFGIGPVFDDGFYYDFDLSVSISQSDFPKIEKLMKKLVSKNLKIVETDGKNL
DFQNQIYKAELKNDLEKKGEKITYFSIVDSKNNILFEDLCYGNHLESLGKIQNFKLLKIAGAYWKGDSKNKQLTRIYGTS
WDSKENLDKFLGILQERQERDHRKIGAKLKLFTFSSYFGHGFPVWLENGMRIYNKIRQKILEFDRNYGFKEVLTPHFGHK
ELYKISGHLLHYRDDMFKGLKIENEELIPRPMTCPHHIILFSQQLFSYRNLPYRISEQSRLYRYEKSGALLGLERVRAMD
LTEGHIFVSKSQIFSEISHLFQMISEVLDFFKVQIFYISFSKRDPKNKDKFFQDDQMWEQAEADLKQFLDQNKINYIEKI
GEAAFYGPKIDFQVKTALNKELTISTLQLDFLLPEKFKISFINEYNKKETPILIHRGLIGTYERFIALLLEQSKGNLPFW
ISPKQIIVIPVAEKHNDFAQKIHKILFDLGFNSEIDLRSERISRKIREANLEKINFQVIIGDEEITKNEITYRKFGSPEL
FRISLVDFVDLLKNSEKNV

Sequences:

>Translated_579_residues
MDLKIDYKLNHSSSHLLALAIKILYPGVKFGIGPVFDDGFYYDFDLSVSISQSDFPKIEKLMKKLVSKNLKIVETDGKNL
DFQNQIYKAELKNDLEKKGEKITYFSIVDSKNNILFEDLCYGNHLESLGKIQNFKLLKIAGAY*KGDSKNKQLTRIYGTS
*DSKENLDKFLGILQERQERDHRKIGAKLKLFTFSSYFGHGFPV*LENGMRIYNKIRQKILEFDRNYGFKEVLTPHFGHK
ELYKISGHLLHYRDDMFKGLKIENEELIPRPMTCPHHIILFSQQLFSYRNLPYRISEQSRLYRYEKSGALLGLERVRAMD
LTEGHIFVSKSQIFSEISHLFQMISEVLDFFKVQIFYISFSKRDPKNKDKFFQDDQMWEQAEADLKQFLDQNKINYIEKI
GEAAFYGPKIDFQVKTALNKELTISTLQLDFLLPEKFKISFINEYNKKETPILIHRGLIGTYERFIALLLEQSKGNLPF*
ISPKQIIVIPVAEKHNDFAQKIHKILFDLGFNSEIDLRSERISRKIREANLEKINFQVIIGDEEITKNEITYRKFGSPEL
FRISLVDFVDLLKNSEKNV
>Mature_579_residues
MDLKIDYKLNHSSSHLLALAIKILYPGVKFGIGPVFDDGFYYDFDLSVSISQSDFPKIEKLMKKLVSKNLKIVETDGKNL
DFQNQIYKAELKNDLEKKGEKITYFSIVDSKNNILFEDLCYGNHLESLGKIQNFKLLKIAGAY*KGDSKNKQLTRIYGTS
*DSKENLDKFLGILQERQERDHRKIGAKLKLFTFSSYFGHGFPV*LENGMRIYNKIRQKILEFDRNYGFKEVLTPHFGHK
ELYKISGHLLHYRDDMFKGLKIENEELIPRPMTCPHHIILFSQQLFSYRNLPYRISEQSRLYRYEKSGALLGLERVRAMD
LTEGHIFVSKSQIFSEISHLFQMISEVLDFFKVQIFYISFSKRDPKNKDKFFQDDQMWEQAEADLKQFLDQNKINYIEKI
GEAAFYGPKIDFQVKTALNKELTISTLQLDFLLPEKFKISFINEYNKKETPILIHRGLIGTYERFIALLLEQSKGNLPF*
ISPKQIIVIPVAEKHNDFAQKIHKILFDLGFNSEIDLRSERISRKIREANLEKINFQVIIGDEEITKNEITYRKFGSPEL
FRISLVDFVDLLKNSEKNV

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=551, Percent_Identity=33.5753176043557, Blast_Score=295, Evalue=8e-80,
Organism=Homo sapiens, GI38570111, Length=577, Percent_Identity=32.9289428076257, Blast_Score=284, Evalue=2e-76,
Organism=Homo sapiens, GI20070344, Length=567, Percent_Identity=31.5696649029982, Blast_Score=258, Evalue=9e-69,
Organism=Escherichia coli, GI1788013, Length=574, Percent_Identity=33.4494773519164, Blast_Score=353, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI71984184, Length=545, Percent_Identity=33.7614678899083, Blast_Score=302, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI71984192, Length=545, Percent_Identity=33.7614678899083, Blast_Score=302, Evalue=3e-82,
Organism=Saccharomyces cerevisiae, GI6322112, Length=560, Percent_Identity=33.5714285714286, Blast_Score=315, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6322654, Length=369, Percent_Identity=36.8563685636856, Blast_Score=223, Evalue=6e-59,
Organism=Drosophila melanogaster, GI24583839, Length=569, Percent_Identity=33.3919156414763, Blast_Score=308, Evalue=9e-84,
Organism=Drosophila melanogaster, GI24583842, Length=569, Percent_Identity=33.3919156414763, Blast_Score=308, Evalue=1e-83,
Organism=Drosophila melanogaster, GI28574141, Length=569, Percent_Identity=33.3919156414763, Blast_Score=308, Evalue=1e-83,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 67235; Mature: 67235

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKIDYKLNHSSSHLLALAIKILYPGVKFGIGPVFDDGFYYDFDLSVSISQSDFPKIEK
CCEEEEEEECCCCCHHHHHHHHHHCCCHHHCCCCEECCCEEEEEEEEEEECCCCCHHHHH
LMKKLVSKNLKIVETDGKNLDFQNQIYKAELKNDLEKKGEKITYFSIVDSKNNILFEDLC
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCEEEEHHH
YGNHLESLGKIQNFKLLKIAGAYKGDSKNKQLTRIYGTSDSKENLDKFLGILQERQERDH
CCCHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
RKIGAKLKLFTFSSYFGHGFPVLENGMRIYNKIRQKILEFDRNYGFKEVLTPHFGHKELY
HHHCCEEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHH
KISGHLLHYRDDMFKGLKIENEELIPRPMTCPHHIILFSQQLFSYRNLPYRISEQSRLYR
HHHCCEEEEHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCHHHHHE
YEKSGALLGLERVRAMDLTEGHIFVSKSQIFSEISHLFQMISEVLDFFKVQIFYISFSKR
ECCCCCEEEHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCC
DPKNKDKFFQDDQMWEQAEADLKQFLDQNKINYIEKIGEAAFYGPKIDFQVKTALNKELT
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEHHHCCCCEE
ISTLQLDFLLPEKFKISFINEYNKKETPILIHRGLIGTYERFIALLLEQSKGNLPFISPK
EEEEEEEEECCCCEEEEEHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCC
QIIVIPVAEKHNDFAQKIHKILFDLGFNSEIDLRSERISRKIREANLEKINFQVIIGDEE
CEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCC
ITKNEITYRKFGSPELFRISLVDFVDLLKNSEKNV
CCCCCCEEEECCCCCEEEEHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDLKIDYKLNHSSSHLLALAIKILYPGVKFGIGPVFDDGFYYDFDLSVSISQSDFPKIEK
CCEEEEEEECCCCCHHHHHHHHHHCCCHHHCCCCEECCCEEEEEEEEEEECCCCCHHHHH
LMKKLVSKNLKIVETDGKNLDFQNQIYKAELKNDLEKKGEKITYFSIVDSKNNILFEDLC
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCEEEEHHH
YGNHLESLGKIQNFKLLKIAGAYKGDSKNKQLTRIYGTSDSKENLDKFLGILQERQERDH
CCCHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
RKIGAKLKLFTFSSYFGHGFPVLENGMRIYNKIRQKILEFDRNYGFKEVLTPHFGHKELY
HHHCCEEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHH
KISGHLLHYRDDMFKGLKIENEELIPRPMTCPHHIILFSQQLFSYRNLPYRISEQSRLYR
HHHCCEEEEHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCHHHHHE
YEKSGALLGLERVRAMDLTEGHIFVSKSQIFSEISHLFQMISEVLDFFKVQIFYISFSKR
ECCCCCEEEHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCC
DPKNKDKFFQDDQMWEQAEADLKQFLDQNKINYIEKIGEAAFYGPKIDFQVKTALNKELT
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEHHHCCCCEE
ISTLQLDFLLPEKFKISFINEYNKKETPILIHRGLIGTYERFIALLLEQSKGNLPFISPK
EEEEEEEEECCCCEEEEEHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCC
QIIVIPVAEKHNDFAQKIHKILFDLGFNSEIDLRSERISRKIREANLEKINFQVIIGDEE
CEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCC
ITKNEITYRKFGSPELFRISLVDFVDLLKNSEKNV
CCCCCCEEEECCCCCEEEEHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11353084 [H]