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The map label for this gene is polA [H]

Identifier: 71893931

GI number: 71893931

Start: 749620

End: 750504

Strand: Reverse

Name: polA [H]

Synonym: MHJ_0582

Alternate gene names: 71893931

Gene position: 750504-749620 (Counterclockwise)

Preceding gene: 71893932

Following gene: 71893930

Centisome position: 83.63

GC content: 25.2

Gene sequence:

>885_bases
ATGGCTAGAAAAATATTATTAATTGATGGAACATGATTAACCTTTAAATCATTTTTTGGTGGATTTCATGGTAATCGCCT
TATTAATTCAAAAGGGGAAATGACCTTTGCAGTTCATATTTTTTTTAGTTCTGTTTTTAAATTATTAAAGCTTCTTAGAC
CTGATAATGTTTATTTTGCTTTTGATTTTGGCGCGAAAACCCCGCGCCATCAAATGTATCCGGATTATAAAAAAGGGCGA
ATAAAACCTCCTGATTCACTTTTTTTTCAAATGGATCAAATTAAAAAAATACTTAGTTTAGCGAACTTTTTATGATCAGA
ACATCAAGATTTTGAAGCCGATGATCTAATAGCATCGCTAAAAAAAAAGATTCAGAAAAAAGATAATGAAGCAGAAATAC
TCATATTTAGTGCAGATCAAGATTTACTACAATTAGTTGATAAAAAAACTAAAGTTATTACAAAAATTAAAAATAATTTC
ATAAATATTAATACACAGGAAAATTTTTATGAATCATACGGTTTTTCGCCCTCTCAAGTGATTGATTTTAAGGTTTTAGC
AGGGGATGTTTCTGATAATATTAAGGTAATTGAGGGCCTAGGCAAAAAAACAGCGATAAAATTACTAGAAAAATACAAAA
ATTTAGATAATATTTTATTAAATCTTGATAAAATTAATCAAAAAATCGCAAATCAAATTAATCAAAAAATAAAACAGTTA
TTATTTTTTAAAAATTTTATTAAATTAAATGATAAGGCTAATTTTGATTTTGATATTTTCCAGAAATTAGACATCAAAAT
TAGTCCATTATTAGTGAAAATTTTAAACGAACTTGAATTAAAAAAAGTATACGAAAATCTAACTGAATTAGCTAGTAAAT
ATTAA

Upstream 100 bases:

>100_bases
ATTATTTATTGCTTGAGACAAAAAAACACTATGAATTTATAGTGTTTTTTTCAAAACCAGGCAAATTCTATTTAAAAGGA
AGTCCAAAAAATTGCTAACT

Downstream 100 bases:

>100_bases
AAAAATAGTATTAATGTAAATATGAAAATTAAAAAATTATTTCTTTTTTCCTCCATCTTTTTAGGTTCTTTAGCCACCGG
ACTCGGAGCTTATTTAATTT

Product: putative 5'-3' exonuclease

Products: NA

Alternate protein names: Tth polymerase 1 [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MARKILLIDGTWLTFKSFFGGFHGNRLINSKGEMTFAVHIFFSSVFKLLKLLRPDNVYFAFDFGAKTPRHQMYPDYKKGR
IKPPDSLFFQMDQIKKILSLANFLWSEHQDFEADDLIASLKKKIQKKDNEAEILIFSADQDLLQLVDKKTKVITKIKNNF
ININTQENFYESYGFSPSQVIDFKVLAGDVSDNIKVIEGLGKKTAIKLLEKYKNLDNILLNLDKINQKIANQINQKIKQL
LFFKNFIKLNDKANFDFDIFQKLDIKISPLLVKILNELELKKVYENLTELASKY

Sequences:

>Translated_294_residues
MARKILLIDGT*LTFKSFFGGFHGNRLINSKGEMTFAVHIFFSSVFKLLKLLRPDNVYFAFDFGAKTPRHQMYPDYKKGR
IKPPDSLFFQMDQIKKILSLANFL*SEHQDFEADDLIASLKKKIQKKDNEAEILIFSADQDLLQLVDKKTKVITKIKNNF
ININTQENFYESYGFSPSQVIDFKVLAGDVSDNIKVIEGLGKKTAIKLLEKYKNLDNILLNLDKINQKIANQINQKIKQL
LFFKNFIKLNDKANFDFDIFQKLDIKISPLLVKILNELELKKVYENLTELASKY
>Mature_293_residues
ARKILLIDGT*LTFKSFFGGFHGNRLINSKGEMTFAVHIFFSSVFKLLKLLRPDNVYFAFDFGAKTPRHQMYPDYKKGRI
KPPDSLFFQMDQIKKILSLANFL*SEHQDFEADDLIASLKKKIQKKDNEAEILIFSADQDLLQLVDKKTKVITKIKNNFI
NINTQENFYESYGFSPSQVIDFKVLAGDVSDNIKVIEGLGKKTAIKLLEKYKNLDNILLNLDKINQKIANQINQKIKQLL
FFKNFIKLNDKANFDFDIFQKLDIKISPLLVKILNELELKKVYENLTELASKY

Specific function: In Addition To Polymerase Activity, This DNA Polymerase Exhibits 3' To 5' And 5' To 3' Exonuclease Activity. It Is Able To Utilize Nicked Circular Duplex DNA As A Template And Can Unwind The Parental DNA Strand From Its Template. [C]

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1790294, Length=220, Percent_Identity=30.4545454545455, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI226510970, Length=194, Percent_Identity=25.7731958762887, Blast_Score=78, Evalue=5e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337
- InterPro:   IPR015361 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A; PF09281 Taq-exonuc [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 33816; Mature: 33685

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKILLIDGTLTFKSFFGGFHGNRLINSKGEMTFAVHIFFSSVFKLLKLLRPDNVYFAF
CCCEEEEEECCHHHHHHHCCCCCCEEECCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEE
DFGAKTPRHQMYPDYKKGRIKPPDSLFFQMDQIKKILSLANFLSEHQDFEADDLIASLKK
ECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
KIQKKDNEAEILIFSADQDLLQLVDKKTKVITKIKNNFININTQENFYESYGFSPSQVID
HHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHCCCCHHHEEE
FKVLAGDVSDNIKVIEGLGKKTAIKLLEKYKNLDNILLNLDKINQKIANQINQKIKQLLF
EEEEECCCCCCHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKNFIKLNDKANFDFDIFQKLDIKISPLLVKILNELELKKVYENLTELASKY
HHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARKILLIDGTLTFKSFFGGFHGNRLINSKGEMTFAVHIFFSSVFKLLKLLRPDNVYFAF
CCEEEEEECCHHHHHHHCCCCCCEEECCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEE
DFGAKTPRHQMYPDYKKGRIKPPDSLFFQMDQIKKILSLANFLSEHQDFEADDLIASLKK
ECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
KIQKKDNEAEILIFSADQDLLQLVDKKTKVITKIKNNFININTQENFYESYGFSPSQVID
HHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHCCCCHHHEEE
FKVLAGDVSDNIKVIEGLGKKTAIKLLEKYKNLDNILLNLDKINQKIANQINQKIKQLLF
EEEEECCCCCCHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKNFIKLNDKANFDFDIFQKLDIKISPLLVKILNELELKKVYENLTELASKY
HHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA