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The map label for this gene is ecfE [H]

Identifier: 71892060

GI number: 71892060

Start: 331484

End: 332857

Strand: Direct

Name: ecfE [H]

Synonym: BPEN_286

Alternate gene names: 71892060

Gene position: 331484-332857 (Clockwise)

Preceding gene: 71892059

Following gene: 71892061

Centisome position: 41.87

GC content: 31.15

Gene sequence:

>1374_bases
ATGTTATTACATTTTTTTTGGAACTTAATCGCATTTATCCTTGCATTAAGTATACTCATCACGGTCCATGAATATGGACA
TTTTGTAGCAGCACGTTTTTTAAAGGTAAAAGTAGAGCGATTTTCTATAGGATTTGGTCCTGTTTTATGGAGTTGGCGAG
ATTCAAATGATACCGAATACGTAATTTCCGCTGTTCTTTTTGGTGGATACGTTAAATTATTTAATACTCGAAAAAAAATA
GCATCTTGCGATGAAGAACGTAATCAATCATTCAATTGTAAACGTATTTGGAAAAAAAGTATTATTGTAGTTTCAGGACC
CATGTTTAACTTTCTTTTTTCTATTGTGTTATACACATTAGTTTTTATGATAGGCGTGCCTATTTATAAACCAATAATCC
ATTCTGTTATACCTAACTCTATTATTGCTCAATCACATGTACCATCTGGTGTAGAAATTAAATCAATTAACAATGTTCTG
ACTCGGGATTGGGATGCAGTACGTTTGGAAATTCTTAATAGCATAGGTAAGGAAAAAATTATTATTTCTGCAACACCAAT
AAATAGTACGCATATTAAAACTTATACCATTAATTTACCTTGCAACTGGTTCAATAAACCTACCAATAATAAAGATCCTA
TAATAACATTGGGTATTCTTCCTTTTAATACACATGTATTACCAATCTTGTCAGGAATACAACCTGATTCTGCCGCACAA
CGAGCTGGTTTAAAAATTGGAGACAAAATAATCTCAATTGACGATCAATTAATACATAATTGGGAGTCATTTATTACAAT
TATTAAAAATAATCCAGAAAAAACTTTTAAAATTATAGTAGAACGGAAAAATAAAATATTAAACTTCAACTTAGCGCCAG
ATAAAAAACATCTGGTTCCTTCCGATAAAGCAGAAGGAGTTATAGGTGTTTTTCCGCAAATTACTTGTATTCCAATAAAA
CATCATGCAATCCATCAATATGGATTGCATCTATCTATACTGGAAGCTTTCGAAAAAACATGGAAATTGATATGCCTAAC
AACTAACACATTATTTAAATTAATTACTGGAGATGTTAGGGTAACCCACCTCAGCGGCCCAATTGCAATAGCGCAAGGAG
CAGGTGCATCCGCTCAATCTGGAGTAATTTACTATCTTATGTTTTTATCTTTAATCAGTATCAATTTAGGTATTATTAAT
TTACTACCGTTTCCAACACTAGATGGAGGGCATTTATTTTTTTTTATAATAGAAAAAATAAAAGGGAAATCAATATCGAA
AGAAACACAGAGTTTTGGTTATATCATTGGATCCATTATACTTACGTTTATGATGTGCTTAGCAATTTTCAACGATATAT
CTAGATTATGGTAA

Upstream 100 bases:

>100_bases
GCATAGACAGCTTATTTGCTGCAGTGCCTATTTTCGTTTACCTAATATTATTAAGTTTATATTAATATCATCATCAAAAA
ATAGAGAATTCATTAACATC

Downstream 100 bases:

>100_bases
TTAATAAAAATTAAAACTAAAAAGATTAAAATAAATAATAATAGATGAAAATAATATTTATAGCATTCTTATTGATAATT
ATCAATGTAGCACATAGCGA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MLLHFFWNLIAFILALSILITVHEYGHFVAARFLKVKVERFSIGFGPVLWSWRDSNDTEYVISAVLFGGYVKLFNTRKKI
ASCDEERNQSFNCKRIWKKSIIVVSGPMFNFLFSIVLYTLVFMIGVPIYKPIIHSVIPNSIIAQSHVPSGVEIKSINNVL
TRDWDAVRLEILNSIGKEKIIISATPINSTHIKTYTINLPCNWFNKPTNNKDPIITLGILPFNTHVLPILSGIQPDSAAQ
RAGLKIGDKIISIDDQLIHNWESFITIIKNNPEKTFKIIVERKNKILNFNLAPDKKHLVPSDKAEGVIGVFPQITCIPIK
HHAIHQYGLHLSILEAFEKTWKLICLTTNTLFKLITGDVRVTHLSGPIAIAQGAGASAQSGVIYYLMFLSLISINLGIIN
LLPFPTLDGGHLFFFIIEKIKGKSISKETQSFGYIIGSIILTFMMCLAIFNDISRLW

Sequences:

>Translated_457_residues
MLLHFFWNLIAFILALSILITVHEYGHFVAARFLKVKVERFSIGFGPVLWSWRDSNDTEYVISAVLFGGYVKLFNTRKKI
ASCDEERNQSFNCKRIWKKSIIVVSGPMFNFLFSIVLYTLVFMIGVPIYKPIIHSVIPNSIIAQSHVPSGVEIKSINNVL
TRDWDAVRLEILNSIGKEKIIISATPINSTHIKTYTINLPCNWFNKPTNNKDPIITLGILPFNTHVLPILSGIQPDSAAQ
RAGLKIGDKIISIDDQLIHNWESFITIIKNNPEKTFKIIVERKNKILNFNLAPDKKHLVPSDKAEGVIGVFPQITCIPIK
HHAIHQYGLHLSILEAFEKTWKLICLTTNTLFKLITGDVRVTHLSGPIAIAQGAGASAQSGVIYYLMFLSLISINLGIIN
LLPFPTLDGGHLFFFIIEKIKGKSISKETQSFGYIIGSIILTFMMCLAIFNDISRLW
>Mature_457_residues
MLLHFFWNLIAFILALSILITVHEYGHFVAARFLKVKVERFSIGFGPVLWSWRDSNDTEYVISAVLFGGYVKLFNTRKKI
ASCDEERNQSFNCKRIWKKSIIVVSGPMFNFLFSIVLYTLVFMIGVPIYKPIIHSVIPNSIIAQSHVPSGVEIKSINNVL
TRDWDAVRLEILNSIGKEKIIISATPINSTHIKTYTINLPCNWFNKPTNNKDPIITLGILPFNTHVLPILSGIQPDSAAQ
RAGLKIGDKIISIDDQLIHNWESFITIIKNNPEKTFKIIVERKNKILNFNLAPDKKHLVPSDKAEGVIGVFPQITCIPIK
HHAIHQYGLHLSILEAFEKTWKLICLTTNTLFKLITGDVRVTHLSGPIAIAQGAGASAQSGVIYYLMFLSLISINLGIIN
LLPFPTLDGGHLFFFIIEKIKGKSISKETQSFGYIIGSIILTFMMCLAIFNDISRLW

Specific function: Cleaves the peptide bond between 'Ala-108' and 'Cys-109' in the transmembrane region of rseA. Acts on degS-cleaved rseA to release the cytoplasmic domain of rseA. This provides the cell with sigma E (rpoE) activity through the proteolysis of rseA. In vitr

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain [H]

Homologues:

Organism=Escherichia coli, GI1786373, Length=455, Percent_Identity=45.4945054945055, Blast_Score=419, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR004387
- InterPro:   IPR008915 [H]

Pfam domain/function: PF00595 PDZ; PF02163 Peptidase_M50 [H]

EC number: NA

Molecular weight: Translated: 51471; Mature: 51471

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLHFFWNLIAFILALSILITVHEYGHFVAARFLKVKVERFSIGFGPVLWSWRDSNDTEY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHEECCCCCEEEEEECCCCHHH
VISAVLFGGYVKLFNTRKKIASCDEERNQSFNCKRIWKKSIIVVSGPMFNFLFSIVLYTL
HHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHCCCEEEEECHHHHHHHHHHHHHH
VFMIGVPIYKPIIHSVIPNSIIAQSHVPSGVEIKSINNVLTRDWDAVRLEILNSIGKEKI
HHHHCCHHHHHHHHHHCCCHHHHHCCCCCCCEEHHHHHHHHCCHHHHHHHHHHHCCCCEE
IISATPINSTHIKTYTINLPCNWFNKPTNNKDPIITLGILPFNTHVLPILSGIQPDSAAQ
EEEECCCCCCEEEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCHHHHHCCCCCCHHHH
RAGLKIGDKIISIDDQLIHNWESFITIIKNNPEKTFKIIVERKNKILNFNLAPDKKHLVP
HCCCCCCCEEEECCHHHHHHHHHHEEEEECCCHHHHHHHEECCCCEEEEECCCCCCCCCC
SDKAEGVIGVFPQITCIPIKHHAIHQYGLHLSILEAFEKTWKLICLTTNTLFKLITGDVR
CCCCCCEEEECCCEEEEECCHHHHHHHCCHHHHHHHHHHCEEEEEEEHHHHHHHHCCCEE
VTHLSGPIAIAQGAGASAQSGVIYYLMFLSLISINLGIINLLPFPTLDGGHLFFFIIEKI
EEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEEEHH
KGKSISKETQSFGYIIGSIILTFMMCLAIFNDISRLW
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLLHFFWNLIAFILALSILITVHEYGHFVAARFLKVKVERFSIGFGPVLWSWRDSNDTEY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHEECCCCCEEEEEECCCCHHH
VISAVLFGGYVKLFNTRKKIASCDEERNQSFNCKRIWKKSIIVVSGPMFNFLFSIVLYTL
HHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHCCCEEEEECHHHHHHHHHHHHHH
VFMIGVPIYKPIIHSVIPNSIIAQSHVPSGVEIKSINNVLTRDWDAVRLEILNSIGKEKI
HHHHCCHHHHHHHHHHCCCHHHHHCCCCCCCEEHHHHHHHHCCHHHHHHHHHHHCCCCEE
IISATPINSTHIKTYTINLPCNWFNKPTNNKDPIITLGILPFNTHVLPILSGIQPDSAAQ
EEEECCCCCCEEEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCHHHHHCCCCCCHHHH
RAGLKIGDKIISIDDQLIHNWESFITIIKNNPEKTFKIIVERKNKILNFNLAPDKKHLVP
HCCCCCCCEEEECCHHHHHHHHHHEEEEECCCHHHHHHHEECCCCEEEEECCCCCCCCCC
SDKAEGVIGVFPQITCIPIKHHAIHQYGLHLSILEAFEKTWKLICLTTNTLFKLITGDVR
CCCCCCEEEECCCEEEEECCHHHHHHHCCHHHHHHHHHHCEEEEEEEHHHHHHHHCCCEE
VTHLSGPIAIAQGAGASAQSGVIYYLMFLSLISINLGIINLLPFPTLDGGHLFFFIIEKI
EEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEEEHH
KGKSISKETQSFGYIIGSIILTFMMCLAIFNDISRLW
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]