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The map label for this gene is pgk

Identifier: 71892035

GI number: 71892035

Start: 297078

End: 298262

Strand: Direct

Name: pgk

Synonym: BPEN_261

Alternate gene names: 71892035

Gene position: 297078-298262 (Clockwise)

Preceding gene: 71892034

Following gene: 71892036

Centisome position: 37.53

GC content: 31.73

Gene sequence:

>1185_bases
ATGACTATGATCAAAATAACTGATTTAGACCTAACGGGTAAACGTGTTTTAATTCGTTCTGATTTAAACGTACCTGTTAA
AAATGGAGTTATTACTTCTAATAGAAAAATTCATGCATCATTACCTACTATTAAATTGGCTTTAAAGAAAAGTAAACATG
TTATGGTAACTTCTCATCTGGGTCGACCAATAGAAGGAGAATATAATGCTCAATTTTCCTTACAGCCCGTGGTTGAATAT
TTAAAGAAACAATTGATTAATCGATATGTTAAAGAAGTGCGTCTGGTTAAAGATTATTTAGAGGGAGTAAACTTTATGGA
AGGAGAGCTTTTAATTTTAGAAAATGTGCGCTTTAATAAAGGAGAAAAAAAAGATGATGAGATATTATCAAAGAAATACG
CCATGTTATGTGATGTATTTATTATGGATGCGTTTGGTAGTGCTCATCGTACACAGGCGTCTACACATGGTATAGGTAAA
TTTGTTTCTTTAGCTTGTTCTGGGCTTTTATTGCAAAAAGAGCTAGAAGCTTTAGGAAAGGCCTTATGTAATCCTATGAG
ACCAATGGTTGCAATAGTTGGGGGATCTAAAGTATCTACTAAATTGATTGTTTTAGATTCTTTATCAAAAGTTGCAGATT
ATTTGATAGTAGGAGGAGGTATTGCAAATACCTTTTTAGCAGCTCAAGGCAAAAAAGTAGGTAAATCCTTATATGAAGAA
GAACTTATACCTACAGCAAAACGTCTTTTAGAAAATTGCGATATACCTATTCTTACTGATGTACGAGTAAGTACAGAATT
TTCTGAAACTGCTCCAGTCACTATGAAAGCTATAATTGATATTAAAGATAATGAGCAAATTCTTGACTTAGGTGATGAAT
CTATTACTCGAATATTAGATATTTTAAATTGTGCTAAAACTATTCTTTGGAACGGTCCTATTGGAGTATTTGAATTTCCA
AATTTCAGAAAAGGCACGGAGATGATATCTCATGCTATTGCTAAAAGTAATGCTTTTTCTATCGTTGGAGGCGGTGATAC
TCTAATGGCTATTGATTTATTTGATATTTCTAATCAAATTTCTTATATTTCTACTGGAGGTGGTGCTTTTTTAGAATTCA
TTGAAGGTAAAACATTTCCATCAGTAAAAATGCTTGAAAAACATGCTTTAAATAACATGAATTAA

Upstream 100 bases:

>100_bases
TAAACTAAACTAAAAATAGTGATATATAGATAAATAAAAATATTTTTCATCATCAATATATGACCATCTATATTGCTTCA
CATTTAAAAGGTTACCTATC

Downstream 100 bases:

>100_bases
CCAGTATTATATATGAAAAATATATACGACTATATTTAAATAGTCTTGTCGATGTATTCTTTCATTAACAACAGAAGAAA
ATAAATGACTAAGATCCTCG

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MTMIKITDLDLTGKRVLIRSDLNVPVKNGVITSNRKIHASLPTIKLALKKSKHVMVTSHLGRPIEGEYNAQFSLQPVVEY
LKKQLINRYVKEVRLVKDYLEGVNFMEGELLILENVRFNKGEKKDDEILSKKYAMLCDVFIMDAFGSAHRTQASTHGIGK
FVSLACSGLLLQKELEALGKALCNPMRPMVAIVGGSKVSTKLIVLDSLSKVADYLIVGGGIANTFLAAQGKKVGKSLYEE
ELIPTAKRLLENCDIPILTDVRVSTEFSETAPVTMKAIIDIKDNEQILDLGDESITRILDILNCAKTILWNGPIGVFEFP
NFRKGTEMISHAIAKSNAFSIVGGGDTLMAIDLFDISNQISYISTGGGAFLEFIEGKTFPSVKMLEKHALNNMN

Sequences:

>Translated_394_residues
MTMIKITDLDLTGKRVLIRSDLNVPVKNGVITSNRKIHASLPTIKLALKKSKHVMVTSHLGRPIEGEYNAQFSLQPVVEY
LKKQLINRYVKEVRLVKDYLEGVNFMEGELLILENVRFNKGEKKDDEILSKKYAMLCDVFIMDAFGSAHRTQASTHGIGK
FVSLACSGLLLQKELEALGKALCNPMRPMVAIVGGSKVSTKLIVLDSLSKVADYLIVGGGIANTFLAAQGKKVGKSLYEE
ELIPTAKRLLENCDIPILTDVRVSTEFSETAPVTMKAIIDIKDNEQILDLGDESITRILDILNCAKTILWNGPIGVFEFP
NFRKGTEMISHAIAKSNAFSIVGGGDTLMAIDLFDISNQISYISTGGGAFLEFIEGKTFPSVKMLEKHALNNMN
>Mature_393_residues
TMIKITDLDLTGKRVLIRSDLNVPVKNGVITSNRKIHASLPTIKLALKKSKHVMVTSHLGRPIEGEYNAQFSLQPVVEYL
KKQLINRYVKEVRLVKDYLEGVNFMEGELLILENVRFNKGEKKDDEILSKKYAMLCDVFIMDAFGSAHRTQASTHGIGKF
VSLACSGLLLQKELEALGKALCNPMRPMVAIVGGSKVSTKLIVLDSLSKVADYLIVGGGIANTFLAAQGKKVGKSLYEEE
LIPTAKRLLENCDIPILTDVRVSTEFSETAPVTMKAIIDIKDNEQILDLGDESITRILDILNCAKTILWNGPIGVFEFPN
FRKGTEMISHAIAKSNAFSIVGGGDTLMAIDLFDISNQISYISTGGGAFLEFIEGKTFPSVKMLEKHALNNMN

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=409, Percent_Identity=35.2078239608802, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI31543397, Length=417, Percent_Identity=35.4916067146283, Blast_Score=224, Evalue=8e-59,
Organism=Escherichia coli, GI1789294, Length=389, Percent_Identity=71.9794344473008, Blast_Score=583, Evalue=1e-168,
Organism=Caenorhabditis elegans, GI17508823, Length=417, Percent_Identity=38.8489208633094, Blast_Score=253, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6319857, Length=413, Percent_Identity=37.2881355932203, Blast_Score=238, Evalue=1e-63,
Organism=Drosophila melanogaster, GI17136394, Length=410, Percent_Identity=40, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24581203, Length=417, Percent_Identity=37.1702637889688, Blast_Score=225, Evalue=4e-59,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_BLOPB (Q493F0)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277765.1
- ProteinModelPortal:   Q493F0
- SMR:   Q493F0
- STRING:   Q493F0
- GeneID:   3562603
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_261
- eggNOG:   COG0126
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- ProtClustDB:   PRK00073
- BioCyc:   CBLO291272:BPEN_261-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 43382; Mature: 43251

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 117-117 BINDING 150-150 BINDING 201-201 BINDING 318-318

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMIKITDLDLTGKRVLIRSDLNVPVKNGVITSNRKIHASLPTIKLALKKSKHVMVTSHL
CEEEEEECCCCCCCEEEEEECCCCCCCCCEEECCCEEEECCCEEEEEECCCCEEEEECCC
GRPIEGEYNAQFSLQPVVEYLKKQLINRYVKEVRLVKDYLEGVNFMEGELLILENVRFNK
CCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCC
GEKKDDEILSKKYAMLCDVFIMDAFGSAHRTQASTHGIGKFVSLACSGLLLQKELEALGK
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALCNPMRPMVAIVGGSKVSTKLIVLDSLSKVADYLIVGGGIANTFLAAQGKKVGKSLYEE
HHCCCCCCEEEEECCCCCCEEEEEEHHHHHHHHHEEECCCHHHHHHHHCCHHHHHHHHHH
ELIPTAKRLLENCDIPILTDVRVSTEFSETAPVTMKAIIDIKDNEQILDLGDESITRILD
HHHHHHHHHHHHCCCCEEECEEEECCCCCCCCEEEEEEEEECCCCEEEECCHHHHHHHHH
ILNCAKTILWNGPIGVFEFPNFRKGTEMISHAIAKSNAFSIVGGGDTLMAIDLFDISNQI
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEECCCCE
SYISTGGGAFLEFIEGKTFPSVKMLEKHALNNMN
EEEECCCHHHHHHHCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
TMIKITDLDLTGKRVLIRSDLNVPVKNGVITSNRKIHASLPTIKLALKKSKHVMVTSHL
EEEEEECCCCCCCEEEEEECCCCCCCCCEEECCCEEEECCCEEEEEECCCCEEEEECCC
GRPIEGEYNAQFSLQPVVEYLKKQLINRYVKEVRLVKDYLEGVNFMEGELLILENVRFNK
CCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCC
GEKKDDEILSKKYAMLCDVFIMDAFGSAHRTQASTHGIGKFVSLACSGLLLQKELEALGK
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALCNPMRPMVAIVGGSKVSTKLIVLDSLSKVADYLIVGGGIANTFLAAQGKKVGKSLYEE
HHCCCCCCEEEEECCCCCCEEEEEEHHHHHHHHHEEECCCHHHHHHHHCCHHHHHHHHHH
ELIPTAKRLLENCDIPILTDVRVSTEFSETAPVTMKAIIDIKDNEQILDLGDESITRILD
HHHHHHHHHHHHCCCCEEECEEEECCCCCCCCEEEEEEEEECCCCEEEECCHHHHHHHHH
ILNCAKTILWNGPIGVFEFPNFRKGTEMISHAIAKSNAFSIVGGGDTLMAIDLFDISNQI
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEECCCCE
SYISTGGGAFLEFIEGKTFPSVKMLEKHALNNMN
EEEECCCHHHHHHHCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA