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The map label for this gene is thiI

Identifier: 71892020

GI number: 71892020

Start: 277383

End: 278843

Strand: Direct

Name: thiI

Synonym: BPEN_246

Alternate gene names: 71892020

Gene position: 277383-278843 (Clockwise)

Preceding gene: 71892017

Following gene: 71892027

Centisome position: 35.04

GC content: 27.24

Gene sequence:

>1461_bases
ATGAAAATAGTTATTAAATTATCACCAGAAATTACAATAAAAAGCCGTGCTATACGAATTTTTTTTATAAAAATCCTCAT
TACAAATATTAAAACTATTTTAAAAAAGAATAATGAATCAGCATCAATTATACGTAATTGGGATTATCTTGAGGTAATAT
GTAATCATAGTAAGTATCAAAAGATATGCGCTATGTTAATAAACATTCCTGGAATTCATCATTTGTTATTAATTAAAAAA
CATATGTTTCGTTCTTTACAAGATATTTATGAAAAAATTATACTCAGTCTGAATTATGAGATTCAATTATCAGGAAAAAG
TTTTTGTGTTCGAGTGAAACGTTGTGGTAAACATAATTTCACTTCTCAAGAAGTTGAACATTATTTAGGGAATAAATTGT
GTCAAAACATAGGTAATATTAGAGTTAACTTAACAAAACCTGAAAAAACTATATATTTGGAAATTAAAGATAATCAACTT
TTTATAATTATCAAGCGTTACGAAGGATTAGGAGGTTTTCCGATAGGTACGCAACAAGAAATGTTGTCATTAATTTCAGG
AGGATTTGATTCAGCTGTTGCTAGTTATATGTTAATTCGGAGAGGATGTAAAGTGAATTATTGTTTTTTTAATTTAGGCG
GAGATATGCATACTGTTGAAGTATGTAGAGTAATATATTTTCTATGGAACAAATTTAGTAGTTCTCATAAAATAAAATTT
ATTTCTATTGATTTTTCAGAAGTCATTAAAGAAATTACTGCTAAAATAAAAGACAACCAAATAGGAGTAGTGTTAAAACG
TATGATGATACGTTCTGCATCATTGGTAGCTAATCGTTATAAAATTACCGCGTTAATAACAGGAGAAGTGTTAGGACAAG
TATCGAGCCAAACTTTGAGCAACCTAACACTTATTGACAGTGTTTCTGATCATGTAATATTTCGTCCTTTAATTGCTTAT
GACAAAGAAAAAATTATCGATTTAGCTAGAAAGATAGGCACAGAAATACTTTCAAAATCTGTTCCAGAATATTGTGGAAT
AATATCAAAAAAATCAACTGCAAAAACAACAAAACAACTTATCGAATTTGAAGAAAATAATTTTGATTTCACCGTGCTAA
ACCGTGCAGTGTCTCAATCTTATGTAATAGACGTACAAAACATTCCTGATCAAATCATTAATCAACATGTATTTCAAGTA
GAAACTAAAAAAATATTGGATTCTACTGATGTAGTATTGGATATACGAACAGAAATTGAACAAGAAAAAAATCCTCTTTA
TTTAAATAATATAGAAATAAAAAAAATACCTTTTTATAATTTAATTGATCAATTTTCTAAATTAGATCCCAATAAAATAT
ATTTATTATATTGTGATCATGGTATAATGAGTCGATTGCAAGTAATGTACTTGCATAGACAAGGATTTAGTAATGTAAAG
ATATACAGGCCACCTAGATAG

Upstream 100 bases:

>100_bases
AGAAGAGCACATTATTATATAAATAATTATAATTTATATAGTATAAGATTACTATATCTAATATATTATGCTTATGTATT
TTAATGTTTAAAATATTTAT

Downstream 100 bases:

>100_bases
CATATTAGGTAAAATAAGTAAGTCTTGGACAAATACTTCAATTTTAAAAAATTGATTAATAACGAGAGCATTGTTTACTT
ACTAATTAAGTAAACTAGAA

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MKIVIKLSPEITIKSRAIRIFFIKILITNIKTILKKNNESASIIRNWDYLEVICNHSKYQKICAMLINIPGIHHLLLIKK
HMFRSLQDIYEKIILSLNYEIQLSGKSFCVRVKRCGKHNFTSQEVEHYLGNKLCQNIGNIRVNLTKPEKTIYLEIKDNQL
FIIIKRYEGLGGFPIGTQQEMLSLISGGFDSAVASYMLIRRGCKVNYCFFNLGGDMHTVEVCRVIYFLWNKFSSSHKIKF
ISIDFSEVIKEITAKIKDNQIGVVLKRMMIRSASLVANRYKITALITGEVLGQVSSQTLSNLTLIDSVSDHVIFRPLIAY
DKEKIIDLARKIGTEILSKSVPEYCGIISKKSTAKTTKQLIEFEENNFDFTVLNRAVSQSYVIDVQNIPDQIINQHVFQV
ETKKILDSTDVVLDIRTEIEQEKNPLYLNNIEIKKIPFYNLIDQFSKLDPNKIYLLYCDHGIMSRLQVMYLHRQGFSNVK
IYRPPR

Sequences:

>Translated_486_residues
MKIVIKLSPEITIKSRAIRIFFIKILITNIKTILKKNNESASIIRNWDYLEVICNHSKYQKICAMLINIPGIHHLLLIKK
HMFRSLQDIYEKIILSLNYEIQLSGKSFCVRVKRCGKHNFTSQEVEHYLGNKLCQNIGNIRVNLTKPEKTIYLEIKDNQL
FIIIKRYEGLGGFPIGTQQEMLSLISGGFDSAVASYMLIRRGCKVNYCFFNLGGDMHTVEVCRVIYFLWNKFSSSHKIKF
ISIDFSEVIKEITAKIKDNQIGVVLKRMMIRSASLVANRYKITALITGEVLGQVSSQTLSNLTLIDSVSDHVIFRPLIAY
DKEKIIDLARKIGTEILSKSVPEYCGIISKKSTAKTTKQLIEFEENNFDFTVLNRAVSQSYVIDVQNIPDQIINQHVFQV
ETKKILDSTDVVLDIRTEIEQEKNPLYLNNIEIKKIPFYNLIDQFSKLDPNKIYLLYCDHGIMSRLQVMYLHRQGFSNVK
IYRPPR
>Mature_486_residues
MKIVIKLSPEITIKSRAIRIFFIKILITNIKTILKKNNESASIIRNWDYLEVICNHSKYQKICAMLINIPGIHHLLLIKK
HMFRSLQDIYEKIILSLNYEIQLSGKSFCVRVKRCGKHNFTSQEVEHYLGNKLCQNIGNIRVNLTKPEKTIYLEIKDNQL
FIIIKRYEGLGGFPIGTQQEMLSLISGGFDSAVASYMLIRRGCKVNYCFFNLGGDMHTVEVCRVIYFLWNKFSSSHKIKF
ISIDFSEVIKEITAKIKDNQIGVVLKRMMIRSASLVANRYKITALITGEVLGQVSSQTLSNLTLIDSVSDHVIFRPLIAY
DKEKIIDLARKIGTEILSKSVPEYCGIISKKSTAKTTKQLIEFEENNFDFTVLNRAVSQSYVIDVQNIPDQIINQHVFQV
ETKKILDSTDVVLDIRTEIEQEKNPLYLNNIEIKKIPFYNLIDQFSKLDPNKIYLLYCDHGIMSRLQVMYLHRQGFSNVK
IYRPPR

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=485, Percent_Identity=52.7835051546392, Blast_Score=537, Evalue=1e-154,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_BLOPB (Q493G5)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277750.1
- ProteinModelPortal:   Q493G5
- SMR:   Q493G5
- STRING:   Q493G5
- GeneID:   3562780
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_246
- eggNOG:   COG0301
- HOGENOM:   HBG646072
- OMA:   GYPIGTQ
- ProtClustDB:   PRK01269
- BioCyc:   CBLO291272:BPEN_246-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR001763
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.250.10
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP; SSF52821 Rhodanese-like

EC number: =2.8.1.4

Molecular weight: Translated: 56165; Mature: 56165

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS50206 RHODANESE_3; PS51165 THUMP

Important sites: ACT_SITE 458-458 BINDING 266-266 BINDING 288-288 BINDING 297-297

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVIKLSPEITIKSRAIRIFFIKILITNIKTILKKNNESASIIRNWDYLEVICNHSKYQ
CEEEEEECCCEEEECCEEEHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHCCHHHH
KICAMLINIPGIHHLLLIKKHMFRSLQDIYEKIILSLNYEIQLSGKSFCVRVKRCGKHNF
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECEEEEECCCHHEEEEHHCCCCCC
TSQEVEHYLGNKLCQNIGNIRVNLTKPEKTIYLEIKDNQLFIIIKRYEGLGGFPIGTQQE
CHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEECCEEEEEEEEECCCCCCCCCCHHH
MLSLISGGFDSAVASYMLIRRGCKVNYCFFNLGGDMHTVEVCRVIYFLWNKFSSSHKIKF
HHHHHHCCHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEE
ISIDFSEVIKEITAKIKDNQIGVVLKRMMIRSASLVANRYKITALITGEVLGQVSSQTLS
EEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEHHHHHHHHHHHHH
NLTLIDSVSDHVIFRPLIAYDKEKIIDLARKIGTEILSKSVPEYCGIISKKSTAKTTKQL
CEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHH
IEFEENNFDFTVLNRAVSQSYVIDVQNIPDQIINQHVFQVETKKILDSTDVVLDIRTEIE
HHHHCCCCCCHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHH
QEKNPLYLNNIEIKKIPFYNLIDQFSKLDPNKIYLLYCDHGIMSRLQVMYLHRQGFSNVK
CCCCCEEEECEEEEECCHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCE
IYRPPR
EECCCC
>Mature Secondary Structure
MKIVIKLSPEITIKSRAIRIFFIKILITNIKTILKKNNESASIIRNWDYLEVICNHSKYQ
CEEEEEECCCEEEECCEEEHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHCCHHHH
KICAMLINIPGIHHLLLIKKHMFRSLQDIYEKIILSLNYEIQLSGKSFCVRVKRCGKHNF
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECEEEEECCCHHEEEEHHCCCCCC
TSQEVEHYLGNKLCQNIGNIRVNLTKPEKTIYLEIKDNQLFIIIKRYEGLGGFPIGTQQE
CHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEECCEEEEEEEEECCCCCCCCCCHHH
MLSLISGGFDSAVASYMLIRRGCKVNYCFFNLGGDMHTVEVCRVIYFLWNKFSSSHKIKF
HHHHHHCCHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEE
ISIDFSEVIKEITAKIKDNQIGVVLKRMMIRSASLVANRYKITALITGEVLGQVSSQTLS
EEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEHHHHHHHHHHHHH
NLTLIDSVSDHVIFRPLIAYDKEKIIDLARKIGTEILSKSVPEYCGIISKKSTAKTTKQL
CEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHH
IEFEENNFDFTVLNRAVSQSYVIDVQNIPDQIINQHVFQVETKKILDSTDVVLDIRTEIE
HHHHCCCCCCHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHH
QEKNPLYLNNIEIKKIPFYNLIDQFSKLDPNKIYLLYCDHGIMSRLQVMYLHRQGFSNVK
CCCCCEEEECEEEEECCHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCE
IYRPPR
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA