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The map label for this gene is birA [H]

Identifier: 71891971

GI number: 71891971

Start: 239424

End: 240239

Strand: Direct

Name: birA [H]

Synonym: BPEN_190

Alternate gene names: 71891971

Gene position: 239424-240239 (Clockwise)

Preceding gene: 71891970

Following gene: 71891973

Centisome position: 30.24

GC content: 29.9

Gene sequence:

>816_bases
TTGTTAAGGGATAATGAAAATTATAGTTTTCCCGTATTTTACCCTTTCTTAAATGAATCTAAGATTTTAACACAATTATC
CGAAGGCAGGGTAGTTGTGTTAAATAGAGTTAATTCAACCAATCAATATATAATCGACAACATTCCATATATAAAATCGG
GTGATGCTTGTGTTACAGAACACCAAACCCAAGGCAGAGGAAGACGTGATAAAATTTGGGTGGCGCCTGTTGGAGAAGGT
ATTTGCTTATCTATTTATTGGAAATTGAATAAGATTCCTTCAACGATGATTGAATTTAGTTTAATGATAAGTATTGTTGT
GGCAAAGATATTAAAGAATTTAGGCGCATCCCAGATTAAAATTAAATGGCCTAATGATTTGTATATTCATGGACGAAAAT
TAGCAGGAATTTTAATCGAAATTATAACAAGAACTGATAATATTACTCATATAATAATAGGTATTGGTATTAATTTATCT
ATACGCACGCATGCAACATTAAATACTAAAATTGGTAAGAATTGGATTAATTTAATGGATATTGGCATCATGATTGATCG
TAATATTTTAGTCGCCACGCTTATTAATGTATTACGTAAGAAATTAAAAGATTTTATGCTTTTTGGGTTTACTCCATTTA
TTACATATTGGAAATTTTTTGATTGCTTATATAATAAACCAGTCACATTATTAGTTGGGAATCGTACAATATGCGGCACA
GCTCTTGGGATAAATATACATGGAGCTTTAATAGTGGATCAAATAAGTTCAGTATGTTATTACTCGGGAAATAATATTTC
TGTTTATACATCTTAA

Upstream 100 bases:

>100_bases
TAATTTTTATCTTAAAATAGAATTAAAAATATATTTACGTAGTATACTGCAATGGAATTAAATATAAATATTAATTTATT
TTTTTGGAGATTTAAAAACC

Downstream 100 bases:

>100_bases
AAATTATATTAACTATAACAATAACCGTTTGATTAACGTAAATCCCATGCTTATTGTAATATAATATGATTAGCTCCAAA
CGTTTCAGTAATAAAAATCG

Product: biotin-[acetylCoA carboxylase] holoenzyme synthetase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MLRDNENYSFPVFYPFLNESKILTQLSEGRVVVLNRVNSTNQYIIDNIPYIKSGDACVTEHQTQGRGRRDKIWVAPVGEG
ICLSIYWKLNKIPSTMIEFSLMISIVVAKILKNLGASQIKIKWPNDLYIHGRKLAGILIEIITRTDNITHIIIGIGINLS
IRTHATLNTKIGKNWINLMDIGIMIDRNILVATLINVLRKKLKDFMLFGFTPFITYWKFFDCLYNKPVTLLVGNRTICGT
ALGINIHGALIVDQISSVCYYSGNNISVYTS

Sequences:

>Translated_271_residues
MLRDNENYSFPVFYPFLNESKILTQLSEGRVVVLNRVNSTNQYIIDNIPYIKSGDACVTEHQTQGRGRRDKIWVAPVGEG
ICLSIYWKLNKIPSTMIEFSLMISIVVAKILKNLGASQIKIKWPNDLYIHGRKLAGILIEIITRTDNITHIIIGIGINLS
IRTHATLNTKIGKNWINLMDIGIMIDRNILVATLINVLRKKLKDFMLFGFTPFITYWKFFDCLYNKPVTLLVGNRTICGT
ALGINIHGALIVDQISSVCYYSGNNISVYTS
>Mature_271_residues
MLRDNENYSFPVFYPFLNESKILTQLSEGRVVVLNRVNSTNQYIIDNIPYIKSGDACVTEHQTQGRGRRDKIWVAPVGEG
ICLSIYWKLNKIPSTMIEFSLMISIVVAKILKNLGASQIKIKWPNDLYIHGRKLAGILIEIITRTDNITHIIIGIGINLS
IRTHATLNTKIGKNWINLMDIGIMIDRNILVATLINVLRKKLKDFMLFGFTPFITYWKFFDCLYNKPVTLLVGNRTICGT
ALGINIHGALIVDQISSVCYYSGNNISVYTS

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=267, Percent_Identity=41.5730337078652, Blast_Score=240, Evalue=8e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR013196
- InterPro:   IPR008988
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB; PF08279 HTH_11 [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 30688; Mature: 30688

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRDNENYSFPVFYPFLNESKILTQLSEGRVVVLNRVNSTNQYIIDNIPYIKSGDACVTE
CCCCCCCCCCEEEEECCCCCHHHHHHCCCCEEEEEECCCCCCEEECCCCEEECCCCEEEC
HQTQGRGRRDKIWVAPVGEGICLSIYWKLNKIPSTMIEFSLMISIVVAKILKNLGASQIK
CHHCCCCCCCEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
IKWPNDLYIHGRKLAGILIEIITRTDNITHIIIGIGINLSIRTHATLNTKIGKNWINLMD
EECCCCEEEECHHHHHHHHHHHHCCCCEEEEEEEEEEEEEEEEEEEEECCCCCCHHEEEE
IGIMIDRNILVATLINVLRKKLKDFMLFGFTPFITYWKFFDCLYNKPVTLLVGNRTICGT
EEEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCEEEEE
ALGINIHGALIVDQISSVCYYSGNNISVYTS
EEEEEECHHHHHHHHHHEEEEECCEEEEEEC
>Mature Secondary Structure
MLRDNENYSFPVFYPFLNESKILTQLSEGRVVVLNRVNSTNQYIIDNIPYIKSGDACVTE
CCCCCCCCCCEEEEECCCCCHHHHHHCCCCEEEEEECCCCCCEEECCCCEEECCCCEEEC
HQTQGRGRRDKIWVAPVGEGICLSIYWKLNKIPSTMIEFSLMISIVVAKILKNLGASQIK
CHHCCCCCCCEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
IKWPNDLYIHGRKLAGILIEIITRTDNITHIIIGIGINLSIRTHATLNTKIGKNWINLMD
EECCCCEEEECHHHHHHHHHHHHCCCCEEEEEEEEEEEEEEEEEEEEECCCCCCHHEEEE
IGIMIDRNILVATLINVLRKKLKDFMLFGFTPFITYWKFFDCLYNKPVTLLVGNRTICGT
EEEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCEEEEE
ALGINIHGALIVDQISSVCYYSGNNISVYTS
EEEEEECHHHHHHHHHHEEEEECCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3899863; 3536662; 8265357; 9278503; 1311302; 1328157; 1409631; 11353844 [H]