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The map label for this gene is clpB [H]

Identifier: 71891969

GI number: 71891969

Start: 235325

End: 237901

Strand: Direct

Name: clpB [H]

Synonym: BPEN_188

Alternate gene names: 71891969

Gene position: 235325-237901 (Clockwise)

Preceding gene: 71891968

Following gene: 71891970

Centisome position: 29.73

GC content: 33.6

Gene sequence:

>2577_bases
ATGCGGTTGGATCATTTTACTCATGATTTTCAATTAGCTATTTCTGATGCTCAATCCATAGCACTTAGAAAAGATAATCA
ATTTGTTGAACCCATTCATGTGATGCTCGCTTTCTTAAAAGAAAAAAATGGATCAGTATATCAGATACTGGAGCGTGCGG
GGTTTAATATGTCTCAGTTCACTATATTAGTTGAAGAGGCATGTGATCGTGTGCCAAAGGTAGAAGGTATTGGGGGAGAT
ATTCAATGTTCTCATGATTTAATTAGAATATTAAATTTATGTAATAAATTAGCACAAAAACATAATGATACTTTTATTGC
GTCAGAAATGTTCGTTCTAGCAGCCATTGAATCTAATGGAACATTAAATGATTTATTAAAAAAATCCGGAGTATCTCCAG
AAAATATAAAAAAATCAACAAGCTATATACGCAATAATCACTTAGTCGATAATCAACAATCAGAATCTCAACGTCAAGCA
TTACAAAAATTTACTGTAGATATAACTAAATTTGCAGAGCAAGGCAATCTTGATCCTGTTATTGGTCGAGATGAAGAAAT
TCGTCGTACTATTCAGGTGTTACAGAGACGTACCAAAAATAATCCTGTTCTTATCGGACAACCTGGAGTAGGAAAAACTG
CTATTGTGGAAGGATTGGCGCAACGTATTATTAATGGTGAAGTTCCGGAAGGACTAAAACATAGTCGCATATTAGCTTTA
GATATGGGAGGACTATTAGCAGGAACAAAATATAGGGGAGAGTTTGAAGAGCGTTTAAAAAGCGTATTAAGTGATGTATC
TAAAGAAGATAGTAATATCATTTTATTCATTGATGAATTGCATACTATGGTTGGAGCAGGTAAAACTGATGGCGCAATGG
ATGCAAGTAATATGCTTAAACCAAGACTATCTAGAGGTGAGTTGCATTGCGTAGGAGCAACTACACTTGATGAATATAGT
AAATATATTGAAAAAGATGCAGCTTTAGAAAGACGTTTTCAAAAAATATTCGTTTCTGAGCCTAGCGTGGAAGATACTAT
AGCTATTCTTCGTGGGTTAAAAGAACGTTACGAATTGCATCACAATGTTCATATTACTGATCCAGCAATAGTGGCTGCTG
CAATTTTATCACATCGATATATTTCTGATCGTCAGTTACCTGATAAAGCCATTGATTTGATTGATGAAGCTGCTTCTAGC
ATTCGCATACAAATAGATTCTAAACCAGAAGAATTAGATAGATTAGAACGGCGTATTATACAGTTGAAATTAGAACAACA
AGCATTAAAAAAAGAATCAGATGATGCTAGCATGAAACGATTAAATCTTTTAACTACTGAGTTAATTCAGAAAGAACAAA
ATTATATTAATCTTGAAAAAGAATGGAAAAAAGAAAAAGTTTCTTTATCTAACGCTCAAAGCATTAAATCGGATTTAGAA
CAAGCAAAAATTGCTATTGACCAAGCACGCCGTGTCGGTGATTTAGCTCGTATGTCTGAATTACAATATGGTAAGATTCC
AGAATTAGAAAAAAAATTATCTACAATCTTGCAATCTAATTATAAAAAGATACGTCTTTTACGAAATCGTGTTACTGATA
TAGAAATTGCCGCAGTATTATCTCGTTGGACTGGTATTCCCGTGTCTCGCATGTTAGCAAGTGAAAAAGATAAATTATTA
GATATGGAACATTCTTTGCATCAATTAGTTGTTGGGCAACACGAAGCAGTAAAGGCAATATCTCATGCTATTCGTCGGAG
TCGTTCAGGGTTATCAGATCTTAAACGTCCAATTGGTTCGTTTATGTTTTTGGGGCCTACTGGAGTAGGAAAAACTGAAT
TGTGCAAGGCATTGTCTATCTTTCTTTTCGATACCAATAATGCAATGGTGCGTATTGACATGTCTGAATTTATGGAAAAG
CATTCTGTATCAAAATTGTTAGGAGCTCCTCCTGGTTATATTGGATATGAATCAGGAGGATATTTGACTGAATCAATACG
TCGTAGACCGTATTCTATTATTTTGTTAGATGAAATAGAAAAAGCGCATTCAGAAATTTTTAATGTACTATTACAAGTCT
TAGATGATGGACGATTAACAGATGGACATGGACGTACGGTACATTTTAATAACACTGTCATCATTATGACATCTAATTTA
GGGTCAGATTTCATTCAAAAACGTTTTGGGATTGTAGATTATCAAGAAGTTAAAGTTGCAGTATTAAACATAGTTAATCA
ACATTTTCGTCCAGAATTTGTTAATAGGATAGATGAAATAGTAGTGTTTCATCCACTTAGCACTGAACATCTTATGAAAA
TTGCTAGTATTCAACTACAGCGCTTATGTGATCGTTTAGAAGAAAAAGGATATCCGAATACTAATATTACTAACGCAGTC
TTGGTATTTTTATCAAAAATTGGATTTGATCCAGTATACGGTGCACGCCCATTAAAGCGTGCTATACAACAACATATTGA
AAATGCATTATCTCAAAAGATAATATCTGGGCAATTGCTGCCAGGTAAAAAAATTACTTTAGATATTGTAAATAATACAA
TCGTCTTTATTCAATGA

Upstream 100 bases:

>100_bases
TGCTTGAATTTTAAGTACCTAATCGCCATATCGATAGGCATATACTGGACTACAGGTATGAAATTGTTTTTATATTATTT
ATAAGAGGAAAATGAATATT

Downstream 100 bases:

>100_bases
AATTTTAAAAATTTCTAACATGAAATTATTTAAGAAATTTTAAATAATTTTAATAAGCAACCATATATACATTGATATAT
ATATGCTCGTTTTTCATGTT

Product: ATP-dependent protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 858; Mature: 858

Protein sequence:

>858_residues
MRLDHFTHDFQLAISDAQSIALRKDNQFVEPIHVMLAFLKEKNGSVYQILERAGFNMSQFTILVEEACDRVPKVEGIGGD
IQCSHDLIRILNLCNKLAQKHNDTFIASEMFVLAAIESNGTLNDLLKKSGVSPENIKKSTSYIRNNHLVDNQQSESQRQA
LQKFTVDITKFAEQGNLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGQPGVGKTAIVEGLAQRIINGEVPEGLKHSRILAL
DMGGLLAGTKYRGEFEERLKSVLSDVSKEDSNIILFIDELHTMVGAGKTDGAMDASNMLKPRLSRGELHCVGATTLDEYS
KYIEKDAALERRFQKIFVSEPSVEDTIAILRGLKERYELHHNVHITDPAIVAAAILSHRYISDRQLPDKAIDLIDEAASS
IRIQIDSKPEELDRLERRIIQLKLEQQALKKESDDASMKRLNLLTTELIQKEQNYINLEKEWKKEKVSLSNAQSIKSDLE
QAKIAIDQARRVGDLARMSELQYGKIPELEKKLSTILQSNYKKIRLLRNRVTDIEIAAVLSRWTGIPVSRMLASEKDKLL
DMEHSLHQLVVGQHEAVKAISHAIRRSRSGLSDLKRPIGSFMFLGPTGVGKTELCKALSIFLFDTNNAMVRIDMSEFMEK
HSVSKLLGAPPGYIGYESGGYLTESIRRRPYSIILLDEIEKAHSEIFNVLLQVLDDGRLTDGHGRTVHFNNTVIIMTSNL
GSDFIQKRFGIVDYQEVKVAVLNIVNQHFRPEFVNRIDEIVVFHPLSTEHLMKIASIQLQRLCDRLEEKGYPNTNITNAV
LVFLSKIGFDPVYGARPLKRAIQQHIENALSQKIISGQLLPGKKITLDIVNNTIVFIQ

Sequences:

>Translated_858_residues
MRLDHFTHDFQLAISDAQSIALRKDNQFVEPIHVMLAFLKEKNGSVYQILERAGFNMSQFTILVEEACDRVPKVEGIGGD
IQCSHDLIRILNLCNKLAQKHNDTFIASEMFVLAAIESNGTLNDLLKKSGVSPENIKKSTSYIRNNHLVDNQQSESQRQA
LQKFTVDITKFAEQGNLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGQPGVGKTAIVEGLAQRIINGEVPEGLKHSRILAL
DMGGLLAGTKYRGEFEERLKSVLSDVSKEDSNIILFIDELHTMVGAGKTDGAMDASNMLKPRLSRGELHCVGATTLDEYS
KYIEKDAALERRFQKIFVSEPSVEDTIAILRGLKERYELHHNVHITDPAIVAAAILSHRYISDRQLPDKAIDLIDEAASS
IRIQIDSKPEELDRLERRIIQLKLEQQALKKESDDASMKRLNLLTTELIQKEQNYINLEKEWKKEKVSLSNAQSIKSDLE
QAKIAIDQARRVGDLARMSELQYGKIPELEKKLSTILQSNYKKIRLLRNRVTDIEIAAVLSRWTGIPVSRMLASEKDKLL
DMEHSLHQLVVGQHEAVKAISHAIRRSRSGLSDLKRPIGSFMFLGPTGVGKTELCKALSIFLFDTNNAMVRIDMSEFMEK
HSVSKLLGAPPGYIGYESGGYLTESIRRRPYSIILLDEIEKAHSEIFNVLLQVLDDGRLTDGHGRTVHFNNTVIIMTSNL
GSDFIQKRFGIVDYQEVKVAVLNIVNQHFRPEFVNRIDEIVVFHPLSTEHLMKIASIQLQRLCDRLEEKGYPNTNITNAV
LVFLSKIGFDPVYGARPLKRAIQQHIENALSQKIISGQLLPGKKITLDIVNNTIVFIQ
>Mature_858_residues
MRLDHFTHDFQLAISDAQSIALRKDNQFVEPIHVMLAFLKEKNGSVYQILERAGFNMSQFTILVEEACDRVPKVEGIGGD
IQCSHDLIRILNLCNKLAQKHNDTFIASEMFVLAAIESNGTLNDLLKKSGVSPENIKKSTSYIRNNHLVDNQQSESQRQA
LQKFTVDITKFAEQGNLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGQPGVGKTAIVEGLAQRIINGEVPEGLKHSRILAL
DMGGLLAGTKYRGEFEERLKSVLSDVSKEDSNIILFIDELHTMVGAGKTDGAMDASNMLKPRLSRGELHCVGATTLDEYS
KYIEKDAALERRFQKIFVSEPSVEDTIAILRGLKERYELHHNVHITDPAIVAAAILSHRYISDRQLPDKAIDLIDEAASS
IRIQIDSKPEELDRLERRIIQLKLEQQALKKESDDASMKRLNLLTTELIQKEQNYINLEKEWKKEKVSLSNAQSIKSDLE
QAKIAIDQARRVGDLARMSELQYGKIPELEKKLSTILQSNYKKIRLLRNRVTDIEIAAVLSRWTGIPVSRMLASEKDKLL
DMEHSLHQLVVGQHEAVKAISHAIRRSRSGLSDLKRPIGSFMFLGPTGVGKTELCKALSIFLFDTNNAMVRIDMSEFMEK
HSVSKLLGAPPGYIGYESGGYLTESIRRRPYSIILLDEIEKAHSEIFNVLLQVLDDGRLTDGHGRTVHFNNTVIIMTSNL
GSDFIQKRFGIVDYQEVKVAVLNIVNQHFRPEFVNRIDEIVVFHPLSTEHLMKIASIQLQRLCDRLEEKGYPNTNITNAV
LVFLSKIGFDPVYGARPLKRAIQQHIENALSQKIISGQLLPGKKITLDIVNNTIVFIQ

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=316, Percent_Identity=33.5443037974684, Blast_Score=187, Evalue=4e-47,
Organism=Escherichia coli, GI1788943, Length=858, Percent_Identity=71.4452214452214, Blast_Score=1288, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=262, Percent_Identity=52.2900763358779, Blast_Score=278, Evalue=8e-76,
Organism=Saccharomyces cerevisiae, GI6320464, Length=735, Percent_Identity=50.2040816326531, Blast_Score=734, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=857, Percent_Identity=41.7736289381564, Blast_Score=648, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 96842; Mature: 96842

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDHFTHDFQLAISDAQSIALRKDNQFVEPIHVMLAFLKEKNGSVYQILERAGFNMSQF
CCCCCCCCHHHEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
TILVEEACDRVPKVEGIGGDIQCSHDLIRILNLCNKLAQKHNDTFIASEMFVLAAIESNG
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHEEEEEEECCCC
TLNDLLKKSGVSPENIKKSTSYIRNNHLVDNQQSESQRQALQKFTVDITKFAEQGNLDPV
CHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
IGRDEEIRRTIQVLQRRTKNNPVLIGQPGVGKTAIVEGLAQRIINGEVPEGLKHSRILAL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DMGGLLAGTKYRGEFEERLKSVLSDVSKEDSNIILFIDELHTMVGAGKTDGAMDASNMLK
ECCCHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHC
PRLSRGELHCVGATTLDEYSKYIEKDAALERRFQKIFVSEPSVEDTIAILRGLKERYELH
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HNVHITDPAIVAAAILSHRYISDRQLPDKAIDLIDEAASSIRIQIDSKPEELDRLERRII
CCEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHH
QLKLEQQALKKESDDASMKRLNLLTTELIQKEQNYINLEKEWKKEKVSLSNAQSIKSDLE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
QAKIAIDQARRVGDLARMSELQYGKIPELEKKLSTILQSNYKKIRLLRNRVTDIEIAAVL
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHH
SRWTGIPVSRMLASEKDKLLDMEHSLHQLVVGQHEAVKAISHAIRRSRSGLSDLKRPIGS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHC
FMFLGPTGVGKTELCKALSIFLFDTNNAMVRIDMSEFMEKHSVSKLLGAPPGYIGYESGG
EEEECCCCCCHHHHHHHHEEEEEECCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTESIRRRPYSIILLDEIEKAHSEIFNVLLQVLDDGRLTDGHGRTVHFNNTVIIMTSNL
HHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEECCEEEEEECCC
GSDFIQKRFGIVDYQEVKVAVLNIVNQHFRPEFVNRIDEIVVFHPLSTEHLMKIASIQLQ
CHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHH
RLCDRLEEKGYPNTNITNAVLVFLSKIGFDPVYGARPLKRAIQQHIENALSQKIISGQLL
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
PGKKITLDIVNNTIVFIQ
CCCEEEEEEECCEEEEEC
>Mature Secondary Structure
MRLDHFTHDFQLAISDAQSIALRKDNQFVEPIHVMLAFLKEKNGSVYQILERAGFNMSQF
CCCCCCCCHHHEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
TILVEEACDRVPKVEGIGGDIQCSHDLIRILNLCNKLAQKHNDTFIASEMFVLAAIESNG
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHEEEEEEECCCC
TLNDLLKKSGVSPENIKKSTSYIRNNHLVDNQQSESQRQALQKFTVDITKFAEQGNLDPV
CHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
IGRDEEIRRTIQVLQRRTKNNPVLIGQPGVGKTAIVEGLAQRIINGEVPEGLKHSRILAL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DMGGLLAGTKYRGEFEERLKSVLSDVSKEDSNIILFIDELHTMVGAGKTDGAMDASNMLK
ECCCHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHC
PRLSRGELHCVGATTLDEYSKYIEKDAALERRFQKIFVSEPSVEDTIAILRGLKERYELH
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HNVHITDPAIVAAAILSHRYISDRQLPDKAIDLIDEAASSIRIQIDSKPEELDRLERRII
CCEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHH
QLKLEQQALKKESDDASMKRLNLLTTELIQKEQNYINLEKEWKKEKVSLSNAQSIKSDLE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
QAKIAIDQARRVGDLARMSELQYGKIPELEKKLSTILQSNYKKIRLLRNRVTDIEIAAVL
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHH
SRWTGIPVSRMLASEKDKLLDMEHSLHQLVVGQHEAVKAISHAIRRSRSGLSDLKRPIGS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHC
FMFLGPTGVGKTELCKALSIFLFDTNNAMVRIDMSEFMEKHSVSKLLGAPPGYIGYESGG
EEEECCCCCCHHHHHHHHEEEEEECCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTESIRRRPYSIILLDEIEKAHSEIFNVLLQVLDDGRLTDGHGRTVHFNNTVIIMTSNL
HHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEECCEEEEEECCC
GSDFIQKRFGIVDYQEVKVAVLNIVNQHFRPEFVNRIDEIVVFHPLSTEHLMKIASIQLQ
CHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHH
RLCDRLEEKGYPNTNITNAVLVFLSKIGFDPVYGARPLKRAIQQHIENALSQKIISGQLL
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
PGKKITLDIVNNTIVFIQ
CCCEEEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12886019 [H]