Definition Candidatus Blochmannia pennsylvanicus str. BPEN, complete genome.
Accession NC_007292
Length 791,654

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The map label for this gene is murD

Identifier: 71891927

GI number: 71891927

Start: 176458

End: 177780

Strand: Direct

Name: murD

Synonym: BPEN_144

Alternate gene names: 71891927

Gene position: 176458-177780 (Clockwise)

Preceding gene: 71891926

Following gene: 71891928

Centisome position: 22.29

GC content: 32.2

Gene sequence:

>1323_bases
ATGCGTAATTACCGAGGATCAAAAGTTGTAATTATCGGTTTGGGGATTACGGGTTTATCTTGTGTTAATTTTTTTTTAGA
TCGTGGCGTAATACCGAAAGTAATAGATACTCGTATATATCCTCCCGGAATAAAAAAAATACCTCATGTTGTACAGTGTT
ATTTGGGGGCATTTAATGATATATGGTTGTTGAGTGCTACGCTGATTGTAGTTAGCCCTGGAGTACGGTTAGATCATCCA
GTTTTAATTGAAGCTTTAAAATTAGGAATAGAAATTATTGGTGATATTGAATTATTTACTCGTGAAGCAACTGCACCGAT
AATAGCTATTACTGGATCTAATGGAAAAAGTACAGTAACCCAATTGGTCTCCAGAATGGCTAGGATCGCTGGATGGCATG
TTGGTGTAGCAGGAAATATAGGTGTACCTGTGCTTTCTTTATTAAATAAATCGTATCAACTCTATATACTGGAGATTTCA
AGTTTTCAGTTAGATACAACCTATAGTTTACGTGCAATAGCAGCTGCAATTTTAAATGTTAGTGAAGATCATATGGATCA
TTATCCTGGAGGATTAAAGCAATATTGGTTTTCTAAGCAAAGGATTTATAAAAATGCTAAAATTTGCGTGATGAACGCTT
TAGATTTTTTAACGATACCTATATATCATGAATATGATTATTGTATAAGTTTTGGTGAAAACGAAGATTCTGCAGATTAT
TATCTGAAATATTATAAAGGACACACCTGGATAGTTGCATACAATGAATATGTGCTTAATTGTTCTGAAATGAGAATTAA
TAATCGTATTAACTATATAAATGCATTATCTGCTTTAGCATTATCGGATATCATCAAGATTCCTCGTTCTGTTTCTTTAA
AAGTATTGTGCCAATTTTCTGGTTTAGCGCATCGTTGTCAATTGATTTATAAAAATCATGGAGTGAGTTGGATTAATGAT
TCCAAAGCTACTAATGTCAGCGCTACCAAAGAAGCGATCAACAATTTAAAGTTATGCGGTACGTTACATTTAATATTAGG
AGGTGATGGTAAATTAGCTGATTTTTCTTCTTTAAAACATTTGATTAAACAACATGAGATACATCTTTATTGTTTTGGAA
AAGATGGATTATTATTGACGACATTAGGATTTAGTGATGTTATTTTAACAAATACAATGATTCAAGCAATGCGTATTATT
AATCGTCGTATAAAAGCGAAGGATATCGTTTTATTATCTCCGGCTTGTTCTAGTTTAGATCAATTTAAATCATTTGAAAT
GCGTGGACTGATATTTACTTGTTTTGCGCGAGAATTTAGATAA

Upstream 100 bases:

>100_bases
AAAGGTTGTCCAGAACCCCGTATTATAGTGCGATTTTGGATCATATCTTTAATGTTAGTTTTTGTAGGATTAATTACTTT
AAAGATACGGCAATAAAAAT

Downstream 100 bases:

>100_bases
TGAGGCTCTTTAAAACAAAAATTATTAATCGTCCATTAAAATTGGGTAAAAAAAAATCATCAAACATTCATGTATTATAT
GATCGTATATTTTTTTGGTT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MRNYRGSKVVIIGLGITGLSCVNFFLDRGVIPKVIDTRIYPPGIKKIPHVVQCYLGAFNDIWLLSATLIVVSPGVRLDHP
VLIEALKLGIEIIGDIELFTREATAPIIAITGSNGKSTVTQLVSRMARIAGWHVGVAGNIGVPVLSLLNKSYQLYILEIS
SFQLDTTYSLRAIAAAILNVSEDHMDHYPGGLKQYWFSKQRIYKNAKICVMNALDFLTIPIYHEYDYCISFGENEDSADY
YLKYYKGHTWIVAYNEYVLNCSEMRINNRINYINALSALALSDIIKIPRSVSLKVLCQFSGLAHRCQLIYKNHGVSWIND
SKATNVSATKEAINNLKLCGTLHLILGGDGKLADFSSLKHLIKQHEIHLYCFGKDGLLLTTLGFSDVILTNTMIQAMRII
NRRIKAKDIVLLSPACSSLDQFKSFEMRGLIFTCFAREFR

Sequences:

>Translated_440_residues
MRNYRGSKVVIIGLGITGLSCVNFFLDRGVIPKVIDTRIYPPGIKKIPHVVQCYLGAFNDIWLLSATLIVVSPGVRLDHP
VLIEALKLGIEIIGDIELFTREATAPIIAITGSNGKSTVTQLVSRMARIAGWHVGVAGNIGVPVLSLLNKSYQLYILEIS
SFQLDTTYSLRAIAAAILNVSEDHMDHYPGGLKQYWFSKQRIYKNAKICVMNALDFLTIPIYHEYDYCISFGENEDSADY
YLKYYKGHTWIVAYNEYVLNCSEMRINNRINYINALSALALSDIIKIPRSVSLKVLCQFSGLAHRCQLIYKNHGVSWIND
SKATNVSATKEAINNLKLCGTLHLILGGDGKLADFSSLKHLIKQHEIHLYCFGKDGLLLTTLGFSDVILTNTMIQAMRII
NRRIKAKDIVLLSPACSSLDQFKSFEMRGLIFTCFAREFR
>Mature_440_residues
MRNYRGSKVVIIGLGITGLSCVNFFLDRGVIPKVIDTRIYPPGIKKIPHVVQCYLGAFNDIWLLSATLIVVSPGVRLDHP
VLIEALKLGIEIIGDIELFTREATAPIIAITGSNGKSTVTQLVSRMARIAGWHVGVAGNIGVPVLSLLNKSYQLYILEIS
SFQLDTTYSLRAIAAAILNVSEDHMDHYPGGLKQYWFSKQRIYKNAKICVMNALDFLTIPIYHEYDYCISFGENEDSADY
YLKYYKGHTWIVAYNEYVLNCSEMRINNRINYINALSALALSDIIKIPRSVSLKVLCQFSGLAHRCQLIYKNHGVSWIND
SKATNVSATKEAINNLKLCGTLHLILGGDGKLADFSSLKHLIKQHEIHLYCFGKDGLLLTTLGFSDVILTNTMIQAMRII
NRRIKAKDIVLLSPACSSLDQFKSFEMRGLIFTCFAREFR

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=439, Percent_Identity=56.9476082004556, Blast_Score=503, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_BLOPB (Q493Q3)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277657.1
- ProteinModelPortal:   Q493Q3
- SMR:   Q493Q3
- STRING:   Q493Q3
- GeneID:   3562866
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_144
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   LWVDDSK
- ProtClustDB:   PRK03806
- BioCyc:   CBLO291272:BPEN_144-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 49313; Mature: 49313

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MRNYRGSKVVIIGLGITGLSCVNFFLDRGVIPKVIDTRIYPPGIKKIPHVVQCYLGAFND
CCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCH
IWLLSATLIVVSPGVRLDHPVLIEALKLGIEIIGDIELFTREATAPIIAITGSNGKSTVT
HHHHHEEEEEECCCCCCCCHHHHHHHHHCHHEEECHHHEEECCCCCEEEEECCCCHHHHH
QLVSRMARIAGWHVGVAGNIGVPVLSLLNKSYQLYILEISSFQLDTTYSLRAIAAAILNV
HHHHHHHHHCCCEEEECCCCCHHHHHHHCCCEEEEEEEEECEEECCCHHHHHHHHHHHCC
SEDHMDHYPGGLKQYWFSKQRIYKNAKICVMNALDFLTIPIYHEYDYCISFGENEDSADY
CHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCEEEEEECCCCCCCCE
YLKYYKGHTWIVAYNEYVLNCSEMRINNRINYINALSALALSDIIKIPRSVSLKVLCQFS
EEEEECCCEEEEEECCEEEEHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
GLAHRCQLIYKNHGVSWINDSKATNVSATKEAINNLKLCGTLHLILGGDGKLADFSSLKH
CHHHHHHEEEECCCCCEECCCCCCCCHHHHHHHCCEEEEEEEEEEECCCCCCCCHHHHHH
LIKQHEIHLYCFGKDGLLLTTLGFSDVILTNTMIQAMRIINRRIKAKDIVLLSPACSSLD
HHHHCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHH
QFKSFEMRGLIFTCFAREFR
HHHHHHHHHHHEEEHHHHCC
>Mature Secondary Structure
MRNYRGSKVVIIGLGITGLSCVNFFLDRGVIPKVIDTRIYPPGIKKIPHVVQCYLGAFND
CCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCH
IWLLSATLIVVSPGVRLDHPVLIEALKLGIEIIGDIELFTREATAPIIAITGSNGKSTVT
HHHHHEEEEEECCCCCCCCHHHHHHHHHCHHEEECHHHEEECCCCCEEEEECCCCHHHHH
QLVSRMARIAGWHVGVAGNIGVPVLSLLNKSYQLYILEISSFQLDTTYSLRAIAAAILNV
HHHHHHHHHCCCEEEECCCCCHHHHHHHCCCEEEEEEEEECEEECCCHHHHHHHHHHHCC
SEDHMDHYPGGLKQYWFSKQRIYKNAKICVMNALDFLTIPIYHEYDYCISFGENEDSADY
CHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCEEEEEECCCCCCCCE
YLKYYKGHTWIVAYNEYVLNCSEMRINNRINYINALSALALSDIIKIPRSVSLKVLCQFS
EEEEECCCEEEEEECCEEEEHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
GLAHRCQLIYKNHGVSWINDSKATNVSATKEAINNLKLCGTLHLILGGDGKLADFSSLKH
CHHHHHHEEEECCCCCEECCCCCCCCHHHHHHHCCEEEEEEEEEEECCCCCCCCHHHHHH
LIKQHEIHLYCFGKDGLLLTTLGFSDVILTNTMIQAMRIINRRIKAKDIVLLSPACSSLD
HHHHCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHH
QFKSFEMRGLIFTCFAREFR
HHHHHHHHHHHEEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA