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The map label for this gene is ksgA

Identifier: 71891913

GI number: 71891913

Start: 158188

End: 158991

Strand: Reverse

Name: ksgA

Synonym: BPEN_130

Alternate gene names: 71891913

Gene position: 158991-158188 (Counterclockwise)

Preceding gene: 71891914

Following gene: 71891912

Centisome position: 20.08

GC content: 28.23

Gene sequence:

>804_bases
ATGAAAAAAATATACTATAAAAATCATCTTATAAAAAAAAAATGGAGCCAAGTATTCCTTAAAGATCAAAATGTTATTGA
TACTATTGTTAAAACTATTAATCCTAAAAAACATCAAAAAATACTAGAAATTGGACCCGGATTAGGTGCGTTAACTAAAC
AAATTCTAAATATTGCAGATCTCGATTCTTTAATATTAATAGAACGTGATTCAAACTTAGTCAATCGACTAGTTCAAATG
TTCAACAAAAAAATTAATATTCTGCACCAAGATATAATGACAACAAATTTTTTTGATTTATCACATAAAGTTGGTCAAAA
ACTACGATTAATAGGAAATTTACCCTATAACATAGCCACAGAATTAATTGTGTATTTATTCCAATACACTAATGTAATTT
ACGATATGCATTTCATGTTTCAAAAAGAAGTAGCTACACGACTTTACGCAAATCCTAATAAAAAAGAATATGGAAGACTA
AGCATAATAACACAATATCATTGTAAAGTTGTTCCATTGTTAACTATACCAGCAACTTCTTTTTTTCCTATCCCAAAAGT
AGAATCTATGGTAATGCGTCTCCTCCCCCACACAAACACTCCCTATCCAATAGTTAATATTGGAAAATTATCTTCGCTAA
CAAAATTAGCTTTTAGACAAAGACGAAAAACTCTTCGAAACAGCTTATCTACATTTTTTAACGAAACAGAAATAACACAA
AAAGGAATAAATCCGGCACTGCGAGCAGAAAACATTACTATTAATCAATACTGTACATTAGCAAATATGTTAAATAACAA
ATAA

Upstream 100 bases:

>100_bases
GATCACGGTACTGCTCTTGAACTATCTGGAACAGGAACAGCGCTACCTGATAGTATGATTATGGCTATTACAGTTGCAAT
TAAAATGTTGAGTCATCATT

Downstream 100 bases:

>100_bases
ACTTGCTATATGATGTGTTATCATAACTGGTATCATTATTTTATAATTGTATTTCAACTAAAAAAATGTTATAAATCTAT
TTTTATTAAAAAATCATATA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MKKIYYKNHLIKKKWSQVFLKDQNVIDTIVKTINPKKHQKILEIGPGLGALTKQILNIADLDSLILIERDSNLVNRLVQM
FNKKINILHQDIMTTNFFDLSHKVGQKLRLIGNLPYNIATELIVYLFQYTNVIYDMHFMFQKEVATRLYANPNKKEYGRL
SIITQYHCKVVPLLTIPATSFFPIPKVESMVMRLLPHTNTPYPIVNIGKLSSLTKLAFRQRRKTLRNSLSTFFNETEITQ
KGINPALRAENITINQYCTLANMLNNK

Sequences:

>Translated_267_residues
MKKIYYKNHLIKKKWSQVFLKDQNVIDTIVKTINPKKHQKILEIGPGLGALTKQILNIADLDSLILIERDSNLVNRLVQM
FNKKINILHQDIMTTNFFDLSHKVGQKLRLIGNLPYNIATELIVYLFQYTNVIYDMHFMFQKEVATRLYANPNKKEYGRL
SIITQYHCKVVPLLTIPATSFFPIPKVESMVMRLLPHTNTPYPIVNIGKLSSLTKLAFRQRRKTLRNSLSTFFNETEITQ
KGINPALRAENITINQYCTLANMLNNK
>Mature_267_residues
MKKIYYKNHLIKKKWSQVFLKDQNVIDTIVKTINPKKHQKILEIGPGLGALTKQILNIADLDSLILIERDSNLVNRLVQM
FNKKINILHQDIMTTNFFDLSHKVGQKLRLIGNLPYNIATELIVYLFQYTNVIYDMHFMFQKEVATRLYANPNKKEYGRL
SIITQYHCKVVPLLTIPATSFFPIPKVESMVMRLLPHTNTPYPIVNIGKLSSLTKLAFRQRRKTLRNSLSTFFNETEITQ
KGINPALRAENITINQYCTLANMLNNK

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=286, Percent_Identity=29.7202797202797, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI7657198, Length=232, Percent_Identity=31.0344827586207, Blast_Score=96, Evalue=2e-20,
Organism=Escherichia coli, GI1786236, Length=261, Percent_Identity=51.7241379310345, Blast_Score=283, Evalue=6e-78,
Organism=Caenorhabditis elegans, GI25146882, Length=217, Percent_Identity=32.7188940092166, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25141369, Length=292, Percent_Identity=28.0821917808219, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6324989, Length=230, Percent_Identity=29.5652173913043, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21358017, Length=220, Percent_Identity=36.3636363636364, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21357273, Length=284, Percent_Identity=28.169014084507, Blast_Score=102, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_BLOPB (Q493R7)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277643.1
- ProteinModelPortal:   Q493R7
- SMR:   Q493R7
- STRING:   Q493R7
- GeneID:   3563098
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_130
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- ProtClustDB:   PRK00274
- BioCyc:   CBLO291272:BPEN_130-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 31009; Mature: 31009

Theoretical pI: Translated: 10.52; Mature: 10.52

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 20-20 BINDING 45-45 BINDING 68-68 BINDING 91-91 BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIYYKNHLIKKKWSQVFLKDQNVIDTIVKTINPKKHQKILEIGPGLGALTKQILNIAD
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHC
LDSLILIERDSNLVNRLVQMFNKKINILHQDIMTTNFFDLSHKVGQKLRLIGNLPYNIAT
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ELIVYLFQYTNVIYDMHFMFQKEVATRLYANPNKKEYGRLSIITQYHCKVVPLLTIPATS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCEEEEEEEECCCC
FFPIPKVESMVMRLLPHTNTPYPIVNIGKLSSLTKLAFRQRRKTLRNSLSTFFNETEITQ
CCCCCHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGINPALRAENITINQYCTLANMLNNK
HCCCHHHHCCCEEHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKIYYKNHLIKKKWSQVFLKDQNVIDTIVKTINPKKHQKILEIGPGLGALTKQILNIAD
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHC
LDSLILIERDSNLVNRLVQMFNKKINILHQDIMTTNFFDLSHKVGQKLRLIGNLPYNIAT
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ELIVYLFQYTNVIYDMHFMFQKEVATRLYANPNKKEYGRLSIITQYHCKVVPLLTIPATS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCEEEEEEEECCCC
FFPIPKVESMVMRLLPHTNTPYPIVNIGKLSSLTKLAFRQRRKTLRNSLSTFFNETEITQ
CCCCCHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGINPALRAENITINQYCTLANMLNNK
HCCCHHHHCCCEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA