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The map label for this gene is ispH

Identifier: 71891907

GI number: 71891907

Start: 149587

End: 150522

Strand: Direct

Name: ispH

Synonym: BPEN_124

Alternate gene names: 71891907

Gene position: 149587-150522 (Clockwise)

Preceding gene: 71891906

Following gene: 71891908

Centisome position: 18.9

GC content: 33.87

Gene sequence:

>936_bases
ATTAAAGTTGTATTAGCTAGTCCGCGTGGGTTCTGCGCAGGGGTAAATCGAGCGATTAATATTGTAGAGAAAACGATACA
AGTATATGGTACTCCAATTTATGTGCGACATGAGTTAGTACATAACGATTATGTGGTAACTCAGTTATCTAAGAAAGGTG
TTATTTTTATTGAGCGGCTAAATGAAGTACCAAATGGATCGGTTCTGATTTTTTCTGCTCACGGAGTGTCAAAAAAGATA
CAGGAGCAATCAAAAATTAAAAATTTAATAATAATTGATGCTACTTGCCCATTGGTAACTAAAGTACATAAGGAAGTCTC
CCGAGCTAATCGTAATGACATGGAAGTAATCTTAATAGGTCATTCTGGTCATCCAGAAGTAGAAGGAACTATGGGACAAT
ATATTTCTGTGAATTCTAGACAAAAAATATATTTAGTTGAGTCGCAAGCAGACGCTTGGTCCGTTCAGGTTAATCAGCCA
AATCATTTATTTTTTGTAACACAAACAACTTTATCTATAGATGATACTGCAGAGATTATAGCAATTTTAAATAAAAGATT
TCCTAACATTATGGGTCCTAGAAAAAATGATATTTGTTACGCAACATTTAATAGACAAAATGCCCTTAAAAATTTAGCTC
CAATGGTAGAGATGATATTTGTTGTAGGGTCAATAACATCATCTAATTCGGTAAGATTAATGGAGCTAGGACGTCGTATT
GGGAAAATTACGTATTTAATTAGTCATGCTAATGATATTCAAAAAAATTGGTTGCGCGGGGTAAATAACATTGGTATTAC
TGCCGGTGCGTCAGCCCCAGACATTTTAGTACTTCAAGTTATTGAAAAATTACGTGTATTAAGCACAGATCATGTTGTTG
TTGAAGAAATGATCGGAGAGGAAGAAAATATATTTTTTGATACACCTAAAATTTAG

Upstream 100 bases:

>100_bases
TAATCTAATTACAAAATTACTAAAAATTAATTGTCACATTTTATGTGAGCAATAAATATAGCAATTTTGTTTACCATGAA
AATACAAAAGGGTATAAATA

Downstream 100 bases:

>100_bases
CGTATTTATAATAATGGATTTGTTGATTTTATTGTAACAGTAACATTACGTATTAAATACAAAGAAGCTGATTATAAAAA
ATTAAAAACGATATGTAACA

Product: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase, 4Fe-4S protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKVVLASPRGFCAGVNRAINIVEKTIQVYGTPIYVRHELVHNDYVVTQLSKKGVIFIERLNEVPNGSVLIFSAHGVSKKI
QEQSKIKNLIIIDATCPLVTKVHKEVSRANRNDMEVILIGHSGHPEVEGTMGQYISVNSRQKIYLVESQADAWSVQVNQP
NHLFFVTQTTLSIDDTAEIIAILNKRFPNIMGPRKNDICYATFNRQNALKNLAPMVEMIFVVGSITSSNSVRLMELGRRI
GKITYLISHANDIQKNWLRGVNNIGITAGASAPDILVLQVIEKLRVLSTDHVVVEEMIGEEENIFFDTPKI

Sequences:

>Translated_311_residues
MKVVLASPRGFCAGVNRAINIVEKTIQVYGTPIYVRHELVHNDYVVTQLSKKGVIFIERLNEVPNGSVLIFSAHGVSKKI
QEQSKIKNLIIIDATCPLVTKVHKEVSRANRNDMEVILIGHSGHPEVEGTMGQYISVNSRQKIYLVESQADAWSVQVNQP
NHLFFVTQTTLSIDDTAEIIAILNKRFPNIMGPRKNDICYATFNRQNALKNLAPMVEMIFVVGSITSSNSVRLMELGRRI
GKITYLISHANDIQKNWLRGVNNIGITAGASAPDILVLQVIEKLRVLSTDHVVVEEMIGEEENIFFDTPKI
>Mature_311_residues
MKVVLASPRGFCAGVNRAINIVEKTIQVYGTPIYVRHELVHNDYVVTQLSKKGVIFIERLNEVPNGSVLIFSAHGVSKKI
QEQSKIKNLIIIDATCPLVTKVHKEVSRANRNDMEVILIGHSGHPEVEGTMGQYISVNSRQKIYLVESQADAWSVQVNQP
NHLFFVTQTTLSIDDTAEIIAILNKRFPNIMGPRKNDICYATFNRQNALKNLAPMVEMIFVVGSITSSNSVRLMELGRRI
GKITYLISHANDIQKNWLRGVNNIGITAGASAPDILVLQVIEKLRVLSTDHVVVEEMIGEEENIFFDTPKI

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=310, Percent_Identity=57.4193548387097, Blast_Score=376, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_BLOPB (Q493S1)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277637.1
- STRING:   Q493S1
- GeneID:   3563127
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_124
- eggNOG:   COG0761
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- BioCyc:   CBLO291272:BPEN_124-MONOMER
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 34741; Mature: 34741

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVLASPRGFCAGVNRAINIVEKTIQVYGTPIYVRHELVHNDYVVTQLSKKGVIFIERL
CEEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEEEECCCCEEEEEEC
NEVPNGSVLIFSAHGVSKKIQEQSKIKNLIIIDATCPLVTKVHKEVSRANRNDMEVILIG
CCCCCCCEEEEECCCCHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEC
HSGHPEVEGTMGQYISVNSRQKIYLVESQADAWSVQVNQPNHLFFVTQTTLSIDDTAEII
CCCCCCCCCCCCCEEEECCCEEEEEEECCCCEEEEEECCCCEEEEEEEEEECCCCHHHHH
AILNKRFPNIMGPRKNDICYATFNRQNALKNLAPMVEMIFVVGSITSSNSVRLMELGRRI
HHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
GKITYLISHANDIQKNWLRGVNNIGITAGASAPDILVLQVIEKLRVLSTDHVVVEEMIGE
HHEEEEEECCHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCC
EENIFFDTPKI
CCCEEEECCCC
>Mature Secondary Structure
MKVVLASPRGFCAGVNRAINIVEKTIQVYGTPIYVRHELVHNDYVVTQLSKKGVIFIERL
CEEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEEEECCCCEEEEEEC
NEVPNGSVLIFSAHGVSKKIQEQSKIKNLIIIDATCPLVTKVHKEVSRANRNDMEVILIG
CCCCCCCEEEEECCCCHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEC
HSGHPEVEGTMGQYISVNSRQKIYLVESQADAWSVQVNQPNHLFFVTQTTLSIDDTAEII
CCCCCCCCCCCCCEEEECCCEEEEEEECCCCEEEEEECCCCEEEEEEEEEECCCCHHHHH
AILNKRFPNIMGPRKNDICYATFNRQNALKNLAPMVEMIFVVGSITSSNSVRLMELGRRI
HHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
GKITYLISHANDIQKNWLRGVNNIGITAGASAPDILVLQVIEKLRVLSTDHVVVEEMIGE
HHEEEEEECCHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCC
EENIFFDTPKI
CCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA