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The map label for this gene is cca

Identifier: 71891853

GI number: 71891853

Start: 75623

End: 76849

Strand: Reverse

Name: cca

Synonym: BPEN_064

Alternate gene names: 71891853

Gene position: 76849-75623 (Counterclockwise)

Preceding gene: 71891857

Following gene: 71891850

Centisome position: 9.71

GC content: 33.66

Gene sequence:

>1227_bases
ATGGAAAAATATTTAGTGGGTGGCGCCGTACGAGACGCGTTGCTAAAACTACCAGTTCAAGAAAAAGATTGGGTAGTAGT
AGGATCTAGTCCTCAAGAAATGTTAAATATAGGCTATGAACAAGTTGGAAAAGATTTTCCGGTATTCTTACACCCAGAAA
GCCATGAAGAATATGCATTGGCACGCACTGAACGAAAATCTGGTCAAGGATATACTGGGTTTATTTGTCATACAGCCCCT
TCAATTACTATTGAAGAAGATCTGTATCGTAGAGATTTAACCATTAATGCTATGGCTTATGACTCACACGGAAATCTTAT
AGATCCATATCACGGACAAAGAGATATAAAATTACGACTGTTACGGCATGTTTCTCATACATTTCATGAAGACCCTCTAC
GAGTATTAAGAGTCGCTCGCTTTGCTGCGCGATTCGCACATATGAATTTTGCTATAGCTCCAGAAACACTAATATTAATG
AAACAAATGATTCATGAATTACTATCGCTATCTCCAGAACGTATATGGACAGAAACAAAAAAAGCTTTGATTACAGATAA
TCCACAAGTATATTTTACAGTCTTACGGCATTGCGGAGCCTTAAAGATTTTATTTCCTGAATTAGATGCATTATTTGATA
TACCTACTCCAACTCAGTATTGCACAAAAATTAACACTGGTTATTATACCATGACAACACTTTCCAAAGCTGCTTATTTA
ACAGATGATATCAGTGTACGTTTTTCTGTTCTATGCCGTGATTTAGGAAAAGGAATCCCTCTCAATAAAATAAACAAAAA
TACCAAACATCATGATCACAGAAAATTGGGAATTACTTTAATTCACAATTTATGCAATAGATTCAAAATACCACATGAAA
TTCGTAATTTTTCAAAAATTACCTCAGAATATCACGACTATTTATATAACGTAAAAATATTAAAACCTGAAATGCTAATG
ACCTTATTTCATGTTTTTGATTGTTGGCGCCGCCCAAACAGAATAGATCAAATTATTTTGGTTAGTCAATCCGATCCAAT
AAGATGGAAGAATTATAATAATTATTCACTCAATCAAGAAAATTTACTACGTACAGCCTTTACCGTTACTAAAAAAATTT
CCACTGTAGACATTATTAAAGATGGATTCACCGGATCGAACATAAGTAGGGAATTATACGCCAGACGTTTACATGCTTTA
AATTCATGGAAAAATAAACAAATATAA

Upstream 100 bases:

>100_bases
CTATATTTTATTTTCAAAATTATATTCTACATAAAATAGTTTTATTTTTAATTAATCAGTAGCATTTTACTATGCAAATC
ATTTGATTGAGAATTGCTCA

Downstream 100 bases:

>100_bases
AATATGTATATACATAATTTTAAAAACACTATAAACAATCGAATAACTTAAAGCAAATATGCACAACTAAAAACAAACCA
CAAACACATATAGTGGCACT

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MEKYLVGGAVRDALLKLPVQEKDWVVVGSSPQEMLNIGYEQVGKDFPVFLHPESHEEYALARTERKSGQGYTGFICHTAP
SITIEEDLYRRDLTINAMAYDSHGNLIDPYHGQRDIKLRLLRHVSHTFHEDPLRVLRVARFAARFAHMNFAIAPETLILM
KQMIHELLSLSPERIWTETKKALITDNPQVYFTVLRHCGALKILFPELDALFDIPTPTQYCTKINTGYYTMTTLSKAAYL
TDDISVRFSVLCRDLGKGIPLNKINKNTKHHDHRKLGITLIHNLCNRFKIPHEIRNFSKITSEYHDYLYNVKILKPEMLM
TLFHVFDCWRRPNRIDQIILVSQSDPIRWKNYNNYSLNQENLLRTAFTVTKKISTVDIIKDGFTGSNISRELYARRLHAL
NSWKNKQI

Sequences:

>Translated_408_residues
MEKYLVGGAVRDALLKLPVQEKDWVVVGSSPQEMLNIGYEQVGKDFPVFLHPESHEEYALARTERKSGQGYTGFICHTAP
SITIEEDLYRRDLTINAMAYDSHGNLIDPYHGQRDIKLRLLRHVSHTFHEDPLRVLRVARFAARFAHMNFAIAPETLILM
KQMIHELLSLSPERIWTETKKALITDNPQVYFTVLRHCGALKILFPELDALFDIPTPTQYCTKINTGYYTMTTLSKAAYL
TDDISVRFSVLCRDLGKGIPLNKINKNTKHHDHRKLGITLIHNLCNRFKIPHEIRNFSKITSEYHDYLYNVKILKPEMLM
TLFHVFDCWRRPNRIDQIILVSQSDPIRWKNYNNYSLNQENLLRTAFTVTKKISTVDIIKDGFTGSNISRELYARRLHAL
NSWKNKQI
>Mature_408_residues
MEKYLVGGAVRDALLKLPVQEKDWVVVGSSPQEMLNIGYEQVGKDFPVFLHPESHEEYALARTERKSGQGYTGFICHTAP
SITIEEDLYRRDLTINAMAYDSHGNLIDPYHGQRDIKLRLLRHVSHTFHEDPLRVLRVARFAARFAHMNFAIAPETLILM
KQMIHELLSLSPERIWTETKKALITDNPQVYFTVLRHCGALKILFPELDALFDIPTPTQYCTKINTGYYTMTTLSKAAYL
TDDISVRFSVLCRDLGKGIPLNKINKNTKHHDHRKLGITLIHNLCNRFKIPHEIRNFSKITSEYHDYLYNVKILKPEMLM
TLFHVFDCWRRPNRIDQIILVSQSDPIRWKNYNNYSLNQENLLRTAFTVTKKISTVDIIKDGFTGSNISRELYARRLHAL
NSWKNKQI

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 2 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=198, Percent_Identity=32.3232323232323, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789436, Length=409, Percent_Identity=53.7897310513447, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI87081691, Length=229, Percent_Identity=29.6943231441048, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321016, Length=152, Percent_Identity=32.2368421052632, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI281360112, Length=210, Percent_Identity=28.0952380952381, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21357337, Length=210, Percent_Identity=28.0952380952381, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_BLOPB (Q493X4)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277582.1
- STRING:   Q493X4
- GeneID:   3563148
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_064
- eggNOG:   COG0617
- HOGENOM:   HBG635063
- OMA:   GHGQKGL
- ProtClustDB:   PRK13298
- BioCyc:   CBLO291272:BPEN_064-MONOMER
- HAMAP:   MF_01262
- InterPro:   IPR012006
- InterPro:   IPR002646
- PIRSF:   PIRSF000813

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 47442; Mature: 47442

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 137-137 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKYLVGGAVRDALLKLPVQEKDWVVVGSSPQEMLNIGYEQVGKDFPVFLHPESHEEYAL
CCCEEECHHHHHHHHHCCCCCCCEEEECCCHHHHHHCCHHHHCCCCCEEECCCCCCHHHH
ARTERKSGQGYTGFICHTAPSITIEEDLYRRDLTINAMAYDSHGNLIDPYHGQRDIKLRL
HHHHHCCCCCCEEEEEECCCCCEEHHHHHHCCEEEEEEEECCCCCCCCCCCCCCHHHHHH
LRHVSHTFHEDPLRVLRVARFAARFAHMNFAIAPETLILMKQMIHELLSLSPERIWTETK
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEECHHHHHHHHHHHHHHHCCCHHHHHHHHH
KALITDNPQVYFTVLRHCGALKILFPELDALFDIPTPTQYCTKINTGYYTMTTLSKAAYL
HHEECCCCHHHHHHHHHCCCEEEEHHCHHHHCCCCCCHHHHHCCCCCEEEEHHHHHHHHC
TDDISVRFSVLCRDLGKGIPLNKINKNTKHHDHRKLGITLIHNLCNRFKIPHEIRNFSKI
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
TSEYHDYLYNVKILKPEMLMTLFHVFDCWRRPNRIDQIILVSQSDPIRWKNYNNYSLNQE
HHHHHHHHEEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEECCCCCCCCCHH
NLLRTAFTVTKKISTVDIIKDGFTGSNISRELYARRLHALNSWKNKQI
HHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MEKYLVGGAVRDALLKLPVQEKDWVVVGSSPQEMLNIGYEQVGKDFPVFLHPESHEEYAL
CCCEEECHHHHHHHHHCCCCCCCEEEECCCHHHHHHCCHHHHCCCCCEEECCCCCCHHHH
ARTERKSGQGYTGFICHTAPSITIEEDLYRRDLTINAMAYDSHGNLIDPYHGQRDIKLRL
HHHHHCCCCCCEEEEEECCCCCEEHHHHHHCCEEEEEEEECCCCCCCCCCCCCCHHHHHH
LRHVSHTFHEDPLRVLRVARFAARFAHMNFAIAPETLILMKQMIHELLSLSPERIWTETK
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEECHHHHHHHHHHHHHHHCCCHHHHHHHHH
KALITDNPQVYFTVLRHCGALKILFPELDALFDIPTPTQYCTKINTGYYTMTTLSKAAYL
HHEECCCCHHHHHHHHHCCCEEEEHHCHHHHCCCCCCHHHHHCCCCCEEEEHHHHHHHHC
TDDISVRFSVLCRDLGKGIPLNKINKNTKHHDHRKLGITLIHNLCNRFKIPHEIRNFSKI
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
TSEYHDYLYNVKILKPEMLMTLFHVFDCWRRPNRIDQIILVSQSDPIRWKNYNNYSLNQE
HHHHHHHHEEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEECCCCCCCCCHH
NLLRTAFTVTKKISTVDIIKDGFTGSNISRELYARRLHALNSWKNKQI
HHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA