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The map label for this gene is cwlM [H]

Identifier: 68537190

GI number: 68537190

Start: 2453346

End: 2454533

Strand: Direct

Name: cwlM [H]

Synonym: jk2095

Alternate gene names: 68537190

Gene position: 2453346-2454533 (Clockwise)

Preceding gene: 68537189

Following gene: NA

Centisome position: 99.63

GC content: 58.16

Gene sequence:

>1188_bases
GTGGATCGTTTGCTTCTCCAGGTCGGCGACCGCAGCCCACGCGTCGCTGAGGTCCGCGGCACCTTGGCTCGCCTCGGGCT
ACTGGAAGGGTACGAAGGTGATGCGACCGGCAGTAAGTCGCAGCGCTGGACCAGTGAAGAAGAAGTCTTCGACGAAACAC
TGGCGGAGGCACTCAAAGCATTTCAGCAGCAACGTGGCATTATTGCCGACGGCACCATCACACCCGGTACTTTGCGGGCG
CTGCGTGAGGCGTCCTACACCCTGGGGGCGCGTGTGCTCTCACTGCAGAGCAACCAGTTCGTGGGGGACGACGTAGCGCA
GCTGCAAACCCAGCTTCACGATCTAGGCTTCTACACCAGCCGCGTGGATGGCCACTTCGGCCCGCGTACCCATTCAGCCG
TGGTGAACTACCAACTGAACTATGGGCTAAACAACGACGGCGTTGTAGGTCCAGATACTCTGCGCGCGCTGTCTTACCTG
GGGCGGAGGATTACCGGTGGCTCACCCCAATCCATTCGGGAAAAGGAACAGATTCGCTCCGCCGGCCCACAGCTTACCGG
CAAGCGCGTTGTGATTGATCCGGGCTTGGGTGGCTCGGACAAGGGTTACATCGTCGATGGTCCTTTCGGGCAGATCTCGG
AGGAGGAAATTCTCTGGGATCTTGCCGGTAGGGTAGAGGGCCGCATGATCGCGGCAGGAATGGAAACGATTCTTTCCCGC
CCGCGTTCGGCAAATCCCACGAATCAGGATCGCGCCAATATCGCTAATGCCTTCGGTGCTGACGTAATGATTAGCCTCCG
CGCCGACATATACCCCAACGATAAGGCCAACGGCGCTGCCACCTTCTACTTCGGATCGCTTACCGGTTATTCGTCAATGG
TGGGCGAGAAGCTTTCCGGCTTTATTCAGCGCGAGATCGTTGCCCGCACACCATTGCAGAACTGCTTTAATCACGGACAG
ACTTGGGACATCCTGCGTCTGACCAGCATGCCGACTGTGCAGGTCGTACCCGGTTATCTGACAAGCCCGAAGGATATTGA
GATCCTCACCGACCCTGCGATGCGCGATACCATCGCCGAAGCGATTGTCGTGGCCGTGAAGCGTCTCTACTTGCTGGACA
AAGATGTCCATGCCACCGGCACCTTCAGCTTCTTGGAGTTGCTACAGGAAGAGCTTCCGAACAAATAG

Upstream 100 bases:

>100_bases
CTCGCCACTGTCGGCGATAGACCCCACATGTGATTTGTCTCACAGTGGTTGCAGCGGCCTTAGAGCCGCAAAAGTTTGAA
GAATTGGAGTGTTGAACCCA

Downstream 100 bases:

>100_bases
TTCCGCCGCCGGGCTGAATTTTGATGACGCCTGGTTAGGCACTTCGCGGTTTCCCAATATCCCAGGCGCATCGAGCGAGT
TTGAAACTTTAGATGTTTGG

Product: putative hydrolase

Products: NA

Alternate protein names: Cell wall hydrolase; Cell wall-associated polypeptide CWBP49; CWBP49; Major autolysin [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MDRLLLQVGDRSPRVAEVRGTLARLGLLEGYEGDATGSKSQRWTSEEEVFDETLAEALKAFQQQRGIIADGTITPGTLRA
LREASYTLGARVLSLQSNQFVGDDVAQLQTQLHDLGFYTSRVDGHFGPRTHSAVVNYQLNYGLNNDGVVGPDTLRALSYL
GRRITGGSPQSIREKEQIRSAGPQLTGKRVVIDPGLGGSDKGYIVDGPFGQISEEEILWDLAGRVEGRMIAAGMETILSR
PRSANPTNQDRANIANAFGADVMISLRADIYPNDKANGAATFYFGSLTGYSSMVGEKLSGFIQREIVARTPLQNCFNHGQ
TWDILRLTSMPTVQVVPGYLTSPKDIEILTDPAMRDTIAEAIVVAVKRLYLLDKDVHATGTFSFLELLQEELPNK

Sequences:

>Translated_395_residues
MDRLLLQVGDRSPRVAEVRGTLARLGLLEGYEGDATGSKSQRWTSEEEVFDETLAEALKAFQQQRGIIADGTITPGTLRA
LREASYTLGARVLSLQSNQFVGDDVAQLQTQLHDLGFYTSRVDGHFGPRTHSAVVNYQLNYGLNNDGVVGPDTLRALSYL
GRRITGGSPQSIREKEQIRSAGPQLTGKRVVIDPGLGGSDKGYIVDGPFGQISEEEILWDLAGRVEGRMIAAGMETILSR
PRSANPTNQDRANIANAFGADVMISLRADIYPNDKANGAATFYFGSLTGYSSMVGEKLSGFIQREIVARTPLQNCFNHGQ
TWDILRLTSMPTVQVVPGYLTSPKDIEILTDPAMRDTIAEAIVVAVKRLYLLDKDVHATGTFSFLELLQEELPNK
>Mature_395_residues
MDRLLLQVGDRSPRVAEVRGTLARLGLLEGYEGDATGSKSQRWTSEEEVFDETLAEALKAFQQQRGIIADGTITPGTLRA
LREASYTLGARVLSLQSNQFVGDDVAQLQTQLHDLGFYTSRVDGHFGPRTHSAVVNYQLNYGLNNDGVVGPDTLRALSYL
GRRITGGSPQSIREKEQIRSAGPQLTGKRVVIDPGLGGSDKGYIVDGPFGQISEEEILWDLAGRVEGRMIAAGMETILSR
PRSANPTNQDRANIANAFGADVMISLRADIYPNDKANGAATFYFGSLTGYSSMVGEKLSGFIQREIVARTPLQNCFNHGQ
TWDILRLTSMPTVQVVPGYLTSPKDIEILTDPAMRDTIAEAIVVAVKRLYLLDKDVHATGTFSFLELLQEELPNK

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. Has a high affinity for teich

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007253
- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3; PF04122 CW_binding_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 43164; Mature: 43164

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRLLLQVGDRSPRVAEVRGTLARLGLLEGYEGDATGSKSQRWTSEEEVFDETLAEALKA
CCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
FQQQRGIIADGTITPGTLRALREASYTLGARVLSLQSNQFVGDDVAQLQTQLHDLGFYTS
HHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHCCHHH
RVDGHFGPRTHSAVVNYQLNYGLNNDGVVGPDTLRALSYLGRRITGGSPQSIREKEQIRS
HCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AGPQLTGKRVVIDPGLGGSDKGYIVDGPFGQISEEEILWDLAGRVEGRMIAAGMETILSR
CCCCCCCCEEEECCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHCC
PRSANPTNQDRANIANAFGADVMISLRADIYPNDKANGAATFYFGSLTGYSSMVGEKLSG
CCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHH
FIQREIVARTPLQNCFNHGQTWDILRLTSMPTVQVVPGYLTSPKDIEILTDPAMRDTIAE
HHHHHHHHHCHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCCCCEEEEECCHHHHHHHH
AIVVAVKRLYLLDKDVHATGTFSFLELLQEELPNK
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDRLLLQVGDRSPRVAEVRGTLARLGLLEGYEGDATGSKSQRWTSEEEVFDETLAEALKA
CCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
FQQQRGIIADGTITPGTLRALREASYTLGARVLSLQSNQFVGDDVAQLQTQLHDLGFYTS
HHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHCCHHH
RVDGHFGPRTHSAVVNYQLNYGLNNDGVVGPDTLRALSYLGRRITGGSPQSIREKEQIRS
HCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AGPQLTGKRVVIDPGLGGSDKGYIVDGPFGQISEEEILWDLAGRVEGRMIAAGMETILSR
CCCCCCCCEEEECCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHCC
PRSANPTNQDRANIANAFGADVMISLRADIYPNDKANGAATFYFGSLTGYSSMVGEKLSG
CCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHH
FIQREIVARTPLQNCFNHGQTWDILRLTSMPTVQVVPGYLTSPKDIEILTDPAMRDTIAE
HHHHHHHHHCHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCCCCEEEEECCHHHHHHHH
AIVVAVKRLYLLDKDVHATGTFSFLELLQEELPNK
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1357079; 1682302; 9384377 [H]