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The map label for this gene is sseA [H]

Identifier: 68536758

GI number: 68536758

Start: 1946033

End: 1946899

Strand: Direct

Name: sseA [H]

Synonym: jk1668

Alternate gene names: 68536758

Gene position: 1946033-1946899 (Clockwise)

Preceding gene: 68536754

Following gene: 68536759

Centisome position: 79.03

GC content: 61.01

Gene sequence:

>867_bases
ATGGGAATCGAATACGACCCCAGCCCTCAGCTCAAGCAGTACGCGCACCCAAACAAGCTGGTCACGAACGCGTGGCTCGG
CGCGAAGCTTGGCACTCCCGGCCTCAAGGTCGTTGAGGTGGATGCGGATTCCCTTTTATACGATATTGGTCACATTCCCA
CCGCAATGCGCCTGAAATGGTACGAAGACCTCTCAGATCCCGTGTGGCGCGACCTTATCGGCCCTAAGGAGTTCGAGGAA
CTCATGGGGCGCCTGGGTATCACACGGGAGGACACGGTGGTGTTTTACGGCGACCACGCGAACCTGTGGGCCGTGTACGC
CCTGTGGGTCTTCGAGCTTTTCGGCCACCCGGATGTGCGAATCCTGGACGGCGGACGAGATGCCTGGATGCAAGAAGAGC
GGGAAATCTCCTACGCCGTCGATGAGGTTTCGCCGCGCCCCGTCTACTCAGTGCCTTCCCACCGGAATGACCACACCCAC
CGCATCTTCGTCGACGAGCTGCGCACGAAGTTCGACGGCACCCAGGTGATTGACCTGCGCAACGCCGAGGAATACACCGG
TGAAGTAACCCTGCCTGGCTACCCAATCCGACGCAACGGACACGTGCCTGGGGCCAAAAACCTGCGCGCAGACTCCTCCC
TGCTAGCTAACTCGCGCTTCCACCCCGCCGAGGCCATCCAGGCTATCCATGCGGAGCTGCAGCTGGACCCCCACCGCGAG
ACCGTCGTTTATTGCAACAACGGTGCACGCGCCGCACTCGAGTGGTTCGTGCTGCGCTTCCTCTTGGGCTGGGAAAATGT
GCGCGTCTACGATGGCTCGTGGGTCGAATGGGGAAACATGATGCGCGTGCCCATCGAGCGTGGCTAA

Upstream 100 bases:

>100_bases
CCAACACCCTTAATTCTACGACTACCCAGCTCAGGTGGCAGTGGAAGAAAGACCGCCGATAAGGCGCTAGACTCTCAAAC
GATCGAAAGGCAGGTGTTAC

Downstream 100 bases:

>100_bases
CTGGGGCGTGTAGCCTCATTAACTAACCCCTAACTAATAACCCGAAAGGATTGCCCGTGACTTCCAGCTCCGCCGCTGAC
TTGACGAAGGTACTGATCGC

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MGIEYDPSPQLKQYAHPNKLVTNAWLGAKLGTPGLKVVEVDADSLLYDIGHIPTAMRLKWYEDLSDPVWRDLIGPKEFEE
LMGRLGITREDTVVFYGDHANLWAVYALWVFELFGHPDVRILDGGRDAWMQEEREISYAVDEVSPRPVYSVPSHRNDHTH
RIFVDELRTKFDGTQVIDLRNAEEYTGEVTLPGYPIRRNGHVPGAKNLRADSSLLANSRFHPAEAIQAIHAELQLDPHRE
TVVYCNNGARAALEWFVLRFLLGWENVRVYDGSWVEWGNMMRVPIERG

Sequences:

>Translated_288_residues
MGIEYDPSPQLKQYAHPNKLVTNAWLGAKLGTPGLKVVEVDADSLLYDIGHIPTAMRLKWYEDLSDPVWRDLIGPKEFEE
LMGRLGITREDTVVFYGDHANLWAVYALWVFELFGHPDVRILDGGRDAWMQEEREISYAVDEVSPRPVYSVPSHRNDHTH
RIFVDELRTKFDGTQVIDLRNAEEYTGEVTLPGYPIRRNGHVPGAKNLRADSSLLANSRFHPAEAIQAIHAELQLDPHRE
TVVYCNNGARAALEWFVLRFLLGWENVRVYDGSWVEWGNMMRVPIERG
>Mature_287_residues
GIEYDPSPQLKQYAHPNKLVTNAWLGAKLGTPGLKVVEVDADSLLYDIGHIPTAMRLKWYEDLSDPVWRDLIGPKEFEEL
MGRLGITREDTVVFYGDHANLWAVYALWVFELFGHPDVRILDGGRDAWMQEEREISYAVDEVSPRPVYSVPSHRNDHTHR
IFVDELRTKFDGTQVIDLRNAEEYTGEVTLPGYPIRRNGHVPGAKNLRADSSLLANSRFHPAEAIQAIHAELQLDPHRET
VVYCNNGARAALEWFVLRFLLGWENVRVYDGSWVEWGNMMRVPIERG

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=286, Percent_Identity=26.9230769230769, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI194473668, Length=289, Percent_Identity=27.681660899654, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI61835204, Length=286, Percent_Identity=27.972027972028, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI194473681, Length=286, Percent_Identity=27.972027972028, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI87082121, Length=265, Percent_Identity=28.6792452830189, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081967, Length=258, Percent_Identity=27.5193798449612, Blast_Score=85, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI115534702, Length=248, Percent_Identity=26.6129032258064, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17559150, Length=256, Percent_Identity=26.953125, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6324825, Length=260, Percent_Identity=28.8461538461538, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 33020; Mature: 32888

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIEYDPSPQLKQYAHPNKLVTNAWLGAKLGTPGLKVVEVDADSLLYDIGHIPTAMRLKW
CCCCCCCCCCHHHHCCCCCEEHHHEECCCCCCCCCEEEEECHHHHHHHHHCCCHHHEEEH
YEDLSDPVWRDLIGPKEFEELMGRLGITREDTVVFYGDHANLWAVYALWVFELFGHPDVR
HHHCCCHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEE
ILDGGRDAWMQEEREISYAVDEVSPRPVYSVPSHRNDHTHRIFVDELRTKFDGTQVIDLR
EEECCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEHHHHHHHCCCEEEEEEC
NAEEYTGEVTLPGYPIRRNGHVPGAKNLRADSSLLANSRFHPAEAIQAIHAELQLDPHRE
CCHHHCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHEEECCCCC
TVVYCNNGARAALEWFVLRFLLGWENVRVYDGSWVEWGNMMRVPIERG
EEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCEEECCCEEEEEECCC
>Mature Secondary Structure 
GIEYDPSPQLKQYAHPNKLVTNAWLGAKLGTPGLKVVEVDADSLLYDIGHIPTAMRLKW
CCCCCCCCCHHHHCCCCCEEHHHEECCCCCCCCCEEEEECHHHHHHHHHCCCHHHEEEH
YEDLSDPVWRDLIGPKEFEELMGRLGITREDTVVFYGDHANLWAVYALWVFELFGHPDVR
HHHCCCHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEE
ILDGGRDAWMQEEREISYAVDEVSPRPVYSVPSHRNDHTHRIFVDELRTKFDGTQVIDLR
EEECCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEHHHHHHHCCCEEEEEEC
NAEEYTGEVTLPGYPIRRNGHVPGAKNLRADSSLLANSRFHPAEAIQAIHAELQLDPHRE
CCHHHCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHEEECCCCC
TVVYCNNGARAALEWFVLRFLLGWENVRVYDGSWVEWGNMMRVPIERG
EEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCEEECCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626; 8772169 [H]