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The map label for this gene is prsA [H]

Identifier: 68536579

GI number: 68536579

Start: 1760016

End: 1760987

Strand: Direct

Name: prsA [H]

Synonym: jk1493

Alternate gene names: 68536579

Gene position: 1760016-1760987 (Clockwise)

Preceding gene: 68536578

Following gene: 68536580

Centisome position: 71.47

GC content: 63.17

Gene sequence:

>972_bases
GTGTCCACCACCCACTGGATCGACAATCAAAAGAACCTGATGCTGTTTAGCGGCAGGGCTCATCCGGAATTGGGGGAGGC
TGTTGCCCGCGAATTGGGCATCGAGGTCACTCCGACCACCGCCCGCGACTTCGCTAACGGCGAGATCTTCGTCCGCTTCG
AAGAGTCCGTCCGCGGTTCCGATGCCTTCGTGCTGCAGTCCCACCCGCAGCCGCTGAATAACTGGCTGATGGAGCAGCTG
ATCATGATCGATGCACTAAAGCGTGGCTCCGCCAAGCGCATCACCGCGATCCTGCCCTTCTACCCCTACGCCCGCCAGGA
CAAGAAGCACCGCGGCCGCGAGCCGATCTCCGCCCGCCTGGTTGCCGACCTGCTGAAGACCGCCGGCGCGGACCGCATCG
TCTCTGTCGATCTGCACACCGACCAGATCCAGGGCTTCTTCGACGGTCCGGTGGATCACATGCACGCCATGCCGATCCTG
ACCGACTATGTAAAGGCCAATTACAACCTGGACAACATCTGCGTGGTCTCCCCTGATGCCGGCCGCGTGAAGGTGGCCGA
GAAGTGGGCCAACGTGCTGGGCGATGCCCCACTGGCGTTCATCCACAAGACCCGCGACGTTGACGTAGCCAACAAGGTCA
CCGCCAACCGCGTGGTCGGCGACGTGAAGGGCCGCACCTGCGTGCTGCTGGACGACATGATCGATACCGGTGGAACCATC
GCCGGTGCCGTCGGCGTGCTGCGTGAGGCTGGTGCTGGCGACGTGATCATCGCCACCACCCACGGCGTATTCAGCGGCCC
GGCACGCGAGCGCCTGAGCAGCTGCGGCGCCCGCGAGATCATCACTACCGATACCCTGCCACAGTCCACCGAGGGCTGGG
ATAACCTGACGGTTCTGCCCATCGCCCCGCTGGTGGCCAAGACCATTCACGAGATCTTCGAGAATGGCTCCGTGACCACA
CTGTTTGAGTAG

Upstream 100 bases:

>100_bases
TACGCTCGTTTTTGTTGATATCTCTGAGCGCGACCTAAAATGGGGAGCGCCTGAATAAAAGTGAATAGTCCAGCTCTTCG
ACCGGAAGGTTAAAGCACAA

Downstream 100 bases:

>100_bases
TTTTAATCCCCGGTTGGGGGTGTGCTAACATACTTTCGAATCTCGGCGAGGGCTGAATTCTTACCCAGAATTTGGCCGTT
ATCGGCGCGAAAAGCACACT

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MSTTHWIDNQKNLMLFSGRAHPELGEAVARELGIEVTPTTARDFANGEIFVRFEESVRGSDAFVLQSHPQPLNNWLMEQL
IMIDALKRGSAKRITAILPFYPYARQDKKHRGREPISARLVADLLKTAGADRIVSVDLHTDQIQGFFDGPVDHMHAMPIL
TDYVKANYNLDNICVVSPDAGRVKVAEKWANVLGDAPLAFIHKTRDVDVANKVTANRVVGDVKGRTCVLLDDMIDTGGTI
AGAVGVLREAGAGDVIIATTHGVFSGPARERLSSCGAREIITTDTLPQSTEGWDNLTVLPIAPLVAKTIHEIFENGSVTT
LFE

Sequences:

>Translated_323_residues
MSTTHWIDNQKNLMLFSGRAHPELGEAVARELGIEVTPTTARDFANGEIFVRFEESVRGSDAFVLQSHPQPLNNWLMEQL
IMIDALKRGSAKRITAILPFYPYARQDKKHRGREPISARLVADLLKTAGADRIVSVDLHTDQIQGFFDGPVDHMHAMPIL
TDYVKANYNLDNICVVSPDAGRVKVAEKWANVLGDAPLAFIHKTRDVDVANKVTANRVVGDVKGRTCVLLDDMIDTGGTI
AGAVGVLREAGAGDVIIATTHGVFSGPARERLSSCGAREIITTDTLPQSTEGWDNLTVLPIAPLVAKTIHEIFENGSVTT
LFE
>Mature_322_residues
STTHWIDNQKNLMLFSGRAHPELGEAVARELGIEVTPTTARDFANGEIFVRFEESVRGSDAFVLQSHPQPLNNWLMEQLI
MIDALKRGSAKRITAILPFYPYARQDKKHRGREPISARLVADLLKTAGADRIVSVDLHTDQIQGFFDGPVDHMHAMPILT
DYVKANYNLDNICVVSPDAGRVKVAEKWANVLGDAPLAFIHKTRDVDVANKVTANRVVGDVKGRTCVLLDDMIDTGGTIA
GAVGVLREAGAGDVIIATTHGVFSGPARERLSSCGAREIITTDTLPQSTEGWDNLTVLPIAPLVAKTIHEIFENGSVTTL
FE

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506129, Length=314, Percent_Identity=43.6305732484076, Blast_Score=253, Evalue=1e-67,
Organism=Homo sapiens, GI4506127, Length=314, Percent_Identity=43.312101910828, Blast_Score=251, Evalue=7e-67,
Organism=Homo sapiens, GI28557709, Length=314, Percent_Identity=42.6751592356688, Blast_Score=248, Evalue=5e-66,
Organism=Homo sapiens, GI84875539, Length=317, Percent_Identity=43.217665615142, Blast_Score=248, Evalue=8e-66,
Organism=Homo sapiens, GI194018537, Length=339, Percent_Identity=31.858407079646, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI4506133, Length=344, Percent_Identity=29.6511627906977, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI310128524, Length=145, Percent_Identity=31.7241379310345, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI310115209, Length=145, Percent_Identity=31.7241379310345, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI310118259, Length=145, Percent_Identity=31.7241379310345, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI310119946, Length=145, Percent_Identity=31.7241379310345, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI1787458, Length=315, Percent_Identity=43.4920634920635, Blast_Score=252, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI25149168, Length=314, Percent_Identity=42.0382165605096, Blast_Score=247, Evalue=6e-66,
Organism=Caenorhabditis elegans, GI17554702, Length=314, Percent_Identity=42.0382165605096, Blast_Score=246, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI17554704, Length=311, Percent_Identity=42.1221864951768, Blast_Score=244, Evalue=3e-65,
Organism=Caenorhabditis elegans, GI71989924, Length=314, Percent_Identity=42.0382165605096, Blast_Score=244, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI17570245, Length=343, Percent_Identity=31.7784256559767, Blast_Score=177, Evalue=7e-45,
Organism=Saccharomyces cerevisiae, GI6319403, Length=320, Percent_Identity=41.875, Blast_Score=248, Evalue=8e-67,
Organism=Saccharomyces cerevisiae, GI6320946, Length=313, Percent_Identity=40.5750798722045, Blast_Score=248, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6321776, Length=315, Percent_Identity=40.6349206349206, Blast_Score=233, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6322667, Length=207, Percent_Identity=41.0628019323672, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6324511, Length=95, Percent_Identity=37.8947368421053, Blast_Score=79, Evalue=9e-16,
Organism=Drosophila melanogaster, GI21355239, Length=314, Percent_Identity=42.9936305732484, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI45551540, Length=337, Percent_Identity=40.3560830860534, Blast_Score=238, Evalue=5e-63,
Organism=Drosophila melanogaster, GI24651458, Length=355, Percent_Identity=30.1408450704225, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24651456, Length=355, Percent_Identity=30.1408450704225, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI281362873, Length=355, Percent_Identity=30.1408450704225, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24651454, Length=355, Percent_Identity=30.1408450704225, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24651462, Length=192, Percent_Identity=35.9375, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24651464, Length=192, Percent_Identity=35.9375, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI45552010, Length=192, Percent_Identity=35.9375, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 35260; Mature: 35128

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00144 ASN_GLN_ASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTHWIDNQKNLMLFSGRAHPELGEAVARELGIEVTPTTARDFANGEIFVRFEESVRGS
CCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCEECCCCHHHCCCCEEEEEEHHCCCCC
DAFVLQSHPQPLNNWLMEQLIMIDALKRGSAKRITAILPFYPYARQDKKHRGREPISARL
CEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHCCCCCHHHHH
VADLLKTAGADRIVSVDLHTDQIQGFFDGPVDHMHAMPILTDYVKANYNLDNICVVSPDA
HHHHHHHCCCCEEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCC
GRVKVAEKWANVLGDAPLAFIHKTRDVDVANKVTANRVVGDVKGRTCVLLDDMIDTGGTI
CCEEHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEECHHCCCCHH
AGAVGVLREAGAGDVIIATTHGVFSGPARERLSSCGAREIITTDTLPQSTEGWDNLTVLP
HHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEEEE
IAPLVAKTIHEIFENGSVTTLFE
HHHHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure 
STTHWIDNQKNLMLFSGRAHPELGEAVARELGIEVTPTTARDFANGEIFVRFEESVRGS
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCEECCCCHHHCCCCEEEEEEHHCCCCC
DAFVLQSHPQPLNNWLMEQLIMIDALKRGSAKRITAILPFYPYARQDKKHRGREPISARL
CEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHCCCCCHHHHH
VADLLKTAGADRIVSVDLHTDQIQGFFDGPVDHMHAMPILTDYVKANYNLDNICVVSPDA
HHHHHHHCCCCEEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCC
GRVKVAEKWANVLGDAPLAFIHKTRDVDVANKVTANRVVGDVKGRTCVLLDDMIDTGGTI
CCEEHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEECHHCCCCHH
AGAVGVLREAGAGDVIIATTHGVFSGPARERLSSCGAREIITTDTLPQSTEGWDNLTVLP
HHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEEEE
IAPLVAKTIHEIFENGSVTTLFE
HHHHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]