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The map label for this gene is emrB [C]

Identifier: 68536542

GI number: 68536542

Start: 1715492

End: 1717081

Strand: Direct

Name: emrB [C]

Synonym: jk1456

Alternate gene names: 68536542

Gene position: 1715492-1717081 (Clockwise)

Preceding gene: 68536541

Following gene: 68536547

Centisome position: 69.66

GC content: 61.07

Gene sequence:

>1590_bases
ATGAAAGATACTCGCGAAGAAGCCGCAGCGGCCAAGGCGCGGCTCGACGCGGCAGAGAAGCAGCGCCAGGAAAACAACAA
CAATGTCCCCCTAGTCTTTGGCTGCCTGATGCTTGGCATGCTGATGTCCTCGCTGGGGCAGATGATCTTCTCCACCGCTC
TGCCCACCCTCGTCGGTGAGCTTGGCGGCGCCAACAAAATGAGTTGGGTCATCACCAGCTTCCTGTTGACCATGACCATC
GGCATGCCCGTCTACGGAAAACTGGGCGACCAGATGGGCCGCAAGCCGCTGTATCTGCTGGCAGTAACTCTGTTCCTGGT
CGGGTCCGTCCTCGGCGCGCTGGCGCAGAGCATGAACATGATGATCGTCGCCCGTGGCGTGCAAGGCCTCGGTGCGGGCG
GCCTGATGATCGGTGCCCAATCCATCATCGCCGACGTGGTTCCCGCCCGTGACCGTGGCCGATACATGGGCATCATGGGC
GCCGTCTTCGGCCTGAGTTCCGTACTGGGGCCTCTGTTGGGCGGCTTCTTCACCGACGGTCCCGGCTGGCGCTGGGCGCT
CTGGTTCAACGTGCCGCTGGCAGTCATTACGATCCTGATGGTTTTCTTCAACCTCAAGCTGCCGAAGCGCGGCTCGCGCG
CACACTTGGACGTCCCCGGTACGGTTTTGATGGCCGGCGCAACCAGCTCGCTGATTCTGCTGCTCACCTGGGGTGGTCGA
GATTACGCCTGGTCGAGCCCCGTCATCATCTGCCTGGGTATCGCGGCAGTCGTCTTGGCCACACTGTTTGTGCTGGTGGA
ACGCAAGGCCAAGGATCCGCTGATCCCGATGCAGCTGTTCACTGTCCGCAATTTCGTGGTCACCACAGCGGCCGGCACGA
TCACGGGTGTGGTCATGTTTGGCGTATTGGCTTACATGCCGACATACATTCAGATGGTTCACGGCATGACCCCCACCAAG
GCCGGTCTCATGATGATTCCGATGATGGCCGGAATGATCGTTACCGCCACCGCCTCCGGCCAGTACGTGTCCCGCACGGG
TCGCTACAAGTGGTTCCCCGTCGCGGGTCTGCTCGTTGCCGCCACGGCGGTTGGTCTTATGGGGCTTTTTGACGCCCACG
ATTCCCTCGTGCACCTCGGTCTTGTTTTGGCTCTCATGGGTGTTGGCCTGGGGCTTTCGATGCAGATCCTGGTGCTGATT
GTGCAGAACTCTTTCCCAATCTCGATGGTGGGAACTGCGACAGCTTCGAATAACTTCTTCCGCCAGATTGGTGGCACGGC
GGGCTCTGCGATTATCGGTTCGCTGTTCACACACCGCGTGGCGGATCTGATGGGTCAGCGGGTGCCCGGCGCGCTCAAGG
AGTTGGGTCCGCAGGGGGCTGAGTTTGCGAAGGACTTCCAAGGCGGTGGCAGTGCGCGGTTGACCCCGGAGATCGTCGAT
ACGCTGCCTGGCCCGTTGCATGACGCGATTATTACCTCGTATAACGACGCCCTCGTCCCAGTTCTCGGGGCTATGGCGCC
GGTGGCCGTGTTGGCTGCGCTGATTCTGGTCTTGGTACGTCAGGACACCCTGAAGGAGACCATCGAGTAG

Upstream 100 bases:

>100_bases
AGGACTTCGACATCGCGCTTGTCGGCCTGCAAAAGATCTTCAGTGCCATCGAACCAGCCACCCCCGAGCCAACAGCAGAA
ACAACCAAGGAGAACCGCCC

Downstream 100 bases:

>100_bases
TCCCCGTTGGGATCCTGCGCTTGGAAAGTTACTTGGTCTCTTCTGCCGATCCCTCAGCGTTGCCCTCGTCGGAGCGCTCC
AGGTTTTCCTGGTAGTACTT

Product: major facilitator superfamily permease

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MKDTREEAAAAKARLDAAEKQRQENNNNVPLVFGCLMLGMLMSSLGQMIFSTALPTLVGELGGANKMSWVITSFLLTMTI
GMPVYGKLGDQMGRKPLYLLAVTLFLVGSVLGALAQSMNMMIVARGVQGLGAGGLMIGAQSIIADVVPARDRGRYMGIMG
AVFGLSSVLGPLLGGFFTDGPGWRWALWFNVPLAVITILMVFFNLKLPKRGSRAHLDVPGTVLMAGATSSLILLLTWGGR
DYAWSSPVIICLGIAAVVLATLFVLVERKAKDPLIPMQLFTVRNFVVTTAAGTITGVVMFGVLAYMPTYIQMVHGMTPTK
AGLMMIPMMAGMIVTATASGQYVSRTGRYKWFPVAGLLVAATAVGLMGLFDAHDSLVHLGLVLALMGVGLGLSMQILVLI
VQNSFPISMVGTATASNNFFRQIGGTAGSAIIGSLFTHRVADLMGQRVPGALKELGPQGAEFAKDFQGGGSARLTPEIVD
TLPGPLHDAIITSYNDALVPVLGAMAPVAVLAALILVLVRQDTLKETIE

Sequences:

>Translated_529_residues
MKDTREEAAAAKARLDAAEKQRQENNNNVPLVFGCLMLGMLMSSLGQMIFSTALPTLVGELGGANKMSWVITSFLLTMTI
GMPVYGKLGDQMGRKPLYLLAVTLFLVGSVLGALAQSMNMMIVARGVQGLGAGGLMIGAQSIIADVVPARDRGRYMGIMG
AVFGLSSVLGPLLGGFFTDGPGWRWALWFNVPLAVITILMVFFNLKLPKRGSRAHLDVPGTVLMAGATSSLILLLTWGGR
DYAWSSPVIICLGIAAVVLATLFVLVERKAKDPLIPMQLFTVRNFVVTTAAGTITGVVMFGVLAYMPTYIQMVHGMTPTK
AGLMMIPMMAGMIVTATASGQYVSRTGRYKWFPVAGLLVAATAVGLMGLFDAHDSLVHLGLVLALMGVGLGLSMQILVLI
VQNSFPISMVGTATASNNFFRQIGGTAGSAIIGSLFTHRVADLMGQRVPGALKELGPQGAEFAKDFQGGGSARLTPEIVD
TLPGPLHDAIITSYNDALVPVLGAMAPVAVLAALILVLVRQDTLKETIE
>Mature_529_residues
MKDTREEAAAAKARLDAAEKQRQENNNNVPLVFGCLMLGMLMSSLGQMIFSTALPTLVGELGGANKMSWVITSFLLTMTI
GMPVYGKLGDQMGRKPLYLLAVTLFLVGSVLGALAQSMNMMIVARGVQGLGAGGLMIGAQSIIADVVPARDRGRYMGIMG
AVFGLSSVLGPLLGGFFTDGPGWRWALWFNVPLAVITILMVFFNLKLPKRGSRAHLDVPGTVLMAGATSSLILLLTWGGR
DYAWSSPVIICLGIAAVVLATLFVLVERKAKDPLIPMQLFTVRNFVVTTAAGTITGVVMFGVLAYMPTYIQMVHGMTPTK
AGLMMIPMMAGMIVTATASGQYVSRTGRYKWFPVAGLLVAATAVGLMGLFDAHDSLVHLGLVLALMGVGLGLSMQILVLI
VQNSFPISMVGTATASNNFFRQIGGTAGSAIIGSLFTHRVADLMGQRVPGALKELGPQGAEFAKDFQGGGSARLTPEIVD
TLPGPLHDAIITSYNDALVPVLGAMAPVAVLAALILVLVRQDTLKETIE

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=438, Percent_Identity=25.3424657534247, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1788710, Length=436, Percent_Identity=26.605504587156, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87081983, Length=423, Percent_Identity=25.7683215130024, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788392, Length=337, Percent_Identity=30.8605341246291, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790195, Length=317, Percent_Identity=27.7602523659306, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1790146, Length=167, Percent_Identity=26.3473053892216, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788509, Length=201, Percent_Identity=26.3681592039801, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323735, Length=309, Percent_Identity=31.0679611650485, Blast_Score=146, Evalue=9e-36,
Organism=Saccharomyces cerevisiae, GI6322958, Length=492, Percent_Identity=25.4065040650407, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6319770, Length=482, Percent_Identity=25.3112033195021, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321663, Length=465, Percent_Identity=23.8709677419355, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6325455, Length=430, Percent_Identity=22.7906976744186, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6319783, Length=371, Percent_Identity=25.8760107816712, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324954, Length=472, Percent_Identity=23.728813559322, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6321747, Length=402, Percent_Identity=22.8855721393035, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001958
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55832; Mature: 55832

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
6.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDTREEAAAAKARLDAAEKQRQENNNNVPLVFGCLMLGMLMSSLGQMIFSTALPTLVGE
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGGANKMSWVITSFLLTMTIGMPVYGKLGDQMGRKPLYLLAVTLFLVGSVLGALAQSMNM
HCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
MIVARGVQGLGAGGLMIGAQSIIADVVPARDRGRYMGIMGAVFGLSSVLGPLLGGFFTDG
EEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
PGWRWALWFNVPLAVITILMVFFNLKLPKRGSRAHLDVPGTVLMAGATSSLILLLTWGGR
CCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCEECCCCCEEEECCCCCEEEEEEECCC
DYAWSSPVIICLGIAAVVLATLFVLVERKAKDPLIPMQLFTVRNFVVTTAAGTITGVVMF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GVLAYMPTYIQMVHGMTPTKAGLMMIPMMAGMIVTATASGQYVSRTGRYKWFPVAGLLVA
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEECCCCHHHCCCCEEEEHHHHHHHH
ATAVGLMGLFDAHDSLVHLGLVLALMGVGLGLSMQILVLIVQNSFPISMVGTATASNNFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHH
RQIGGTAGSAIIGSLFTHRVADLMGQRVPGALKELGPQGAEFAKDFQGGGSARLTPEIVD
HHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHCCCCCCCCCHHHHH
TLPGPLHDAIITSYNDALVPVLGAMAPVAVLAALILVLVRQDTLKETIE
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKDTREEAAAAKARLDAAEKQRQENNNNVPLVFGCLMLGMLMSSLGQMIFSTALPTLVGE
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGGANKMSWVITSFLLTMTIGMPVYGKLGDQMGRKPLYLLAVTLFLVGSVLGALAQSMNM
HCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
MIVARGVQGLGAGGLMIGAQSIIADVVPARDRGRYMGIMGAVFGLSSVLGPLLGGFFTDG
EEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
PGWRWALWFNVPLAVITILMVFFNLKLPKRGSRAHLDVPGTVLMAGATSSLILLLTWGGR
CCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCEECCCCCEEEECCCCCEEEEEEECCC
DYAWSSPVIICLGIAAVVLATLFVLVERKAKDPLIPMQLFTVRNFVVTTAAGTITGVVMF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GVLAYMPTYIQMVHGMTPTKAGLMMIPMMAGMIVTATASGQYVSRTGRYKWFPVAGLLVA
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEECCCCHHHCCCCEEEEHHHHHHHH
ATAVGLMGLFDAHDSLVHLGLVLALMGVGLGLSMQILVLIVQNSFPISMVGTATASNNFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHH
RQIGGTAGSAIIGSLFTHRVADLMGQRVPGALKELGPQGAEFAKDFQGGGSARLTPEIVD
HHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHCCCCCCCCCHHHHH
TLPGPLHDAIITSYNDALVPVLGAMAPVAVLAALILVLVRQDTLKETIE
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]