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The map label for this gene is truB

Identifier: 68536214

GI number: 68536214

Start: 1336152

End: 1337078

Strand: Direct

Name: truB

Synonym: jk1137

Alternate gene names: 68536214

Gene position: 1336152-1337078 (Clockwise)

Preceding gene: 68536211

Following gene: 68536223

Centisome position: 54.26

GC content: 61.6

Gene sequence:

>927_bases
ATGAACCAACGCAGTGCCCGTTCCGTCCTGAGCCGTTCCGGGGTCGTCCTCGTAGATAAGCCCTCCGGCCCCACGTCGCA
CGACATGGTGGCTAAACTTCGCCGGATCATGGGAACGCGCCGAATCGGCCATTCCGGAACCCTCGATCCAATGGCCACTG
GGTTGCTAGTTGTGGGCGTCGAAAGAGGAACAAAGTTCCTGGCCCACGTCGTCACTCACGACAAGCGCTATGAGGCAACC
GTCCGTTTAGGGGTCGCCACCCACACCGATGATGCACAGGGCGACGTGCTTTCCACCGCCTCTCCCCAAGACCTGCAGGC
GCTCACCGAACAGCAGGTCCGCGAGGCCTTCGCTGCGCAGCGGGGCGACATCATGCAACGCCCGACCTCGGTTTCTTCCA
TCAAGATTGACGGCAAGCGCGCCCACGAGCTTGTCCGGGAGGGCCACGACGTAGTGTTGCCCGAGCGTCCCGTCACGATC
TTTTCGTTGGAGGTGCTCGATGTTGTTGTTGATGACGCCACCTCTTGCATCGACGCCCGCATTTCCGTGCACTGCTCCAG
CGGTACATATATCCGGGCGATTGCCCGGGACGTCGGCGAGGCTTTGGGCGTGGGAGGTCACCTGACACAGCTCCGCCGTA
CCTCGGTAGGCCCTTTCGACGTCTCCGAAGCACGGACGTTGGAGCAGCTCGAGGAGGATCCAAGCCTGACCCTCAATCTG
GACGAGGCTATGGTGCGTTGTTTCGATACTCGCGAGATCAGCGAATCGGAGGGCGTTGACCTATCCCTGGGCAAATGGCT
GAAACCCGTCGGCAACAAGGGGGTACGCGCCGCGGTCACTCCATCTGGGCAGGCCATCGCACTGGTCGAAGAAAAGGGCA
AACGAGCTTCCAGCGTGTTCGTCGCCCGCCCAGCAGGTATGGACTAA

Upstream 100 bases:

>100_bases
CGATGGTCACAACGGTGCCACCGGCTGCCAGGTGTTCAGGGATTCTGTCCAGTCCGTACCAGATACTCACACGTTGCATG
CTAGTCGATAGGCTGTGGGT

Downstream 100 bases:

>100_bases
CGCGCCGGAACGGCCGCGGCGGTGATCACGTAGCCGTTGCGTATCGTCCAGCGCCCCTGGATGAACGGAACCGGAACCGG
GCGCGCCAACAAGTAAGAGG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MNQRSARSVLSRSGVVLVDKPSGPTSHDMVAKLRRIMGTRRIGHSGTLDPMATGLLVVGVERGTKFLAHVVTHDKRYEAT
VRLGVATHTDDAQGDVLSTASPQDLQALTEQQVREAFAAQRGDIMQRPTSVSSIKIDGKRAHELVREGHDVVLPERPVTI
FSLEVLDVVVDDATSCIDARISVHCSSGTYIRAIARDVGEALGVGGHLTQLRRTSVGPFDVSEARTLEQLEEDPSLTLNL
DEAMVRCFDTREISESEGVDLSLGKWLKPVGNKGVRAAVTPSGQAIALVEEKGKRASSVFVARPAGMD

Sequences:

>Translated_308_residues
MNQRSARSVLSRSGVVLVDKPSGPTSHDMVAKLRRIMGTRRIGHSGTLDPMATGLLVVGVERGTKFLAHVVTHDKRYEAT
VRLGVATHTDDAQGDVLSTASPQDLQALTEQQVREAFAAQRGDIMQRPTSVSSIKIDGKRAHELVREGHDVVLPERPVTI
FSLEVLDVVVDDATSCIDARISVHCSSGTYIRAIARDVGEALGVGGHLTQLRRTSVGPFDVSEARTLEQLEEDPSLTLNL
DEAMVRCFDTREISESEGVDLSLGKWLKPVGNKGVRAAVTPSGQAIALVEEKGKRASSVFVARPAGMD
>Mature_308_residues
MNQRSARSVLSRSGVVLVDKPSGPTSHDMVAKLRRIMGTRRIGHSGTLDPMATGLLVVGVERGTKFLAHVVTHDKRYEAT
VRLGVATHTDDAQGDVLSTASPQDLQALTEQQVREAFAAQRGDIMQRPTSVSSIKIDGKRAHELVREGHDVVLPERPVTI
FSLEVLDVVVDDATSCIDARISVHCSSGTYIRAIARDVGEALGVGGHLTQLRRTSVGPFDVSEARTLEQLEEDPSLTLNL
DEAMVRCFDTREISESEGVDLSLGKWLKPVGNKGVRAAVTPSGQAIALVEEKGKRASSVFVARPAGMD

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=235, Percent_Identity=35.7446808510638, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI215599015, Length=208, Percent_Identity=34.1346153846154, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI4503337, Length=208, Percent_Identity=34.1346153846154, Blast_Score=86, Evalue=6e-17,
Organism=Escherichia coli, GI2367200, Length=220, Percent_Identity=39.0909090909091, Blast_Score=158, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17553978, Length=223, Percent_Identity=28.6995515695067, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323204, Length=215, Percent_Identity=31.6279069767442, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324037, Length=138, Percent_Identity=38.4057971014493, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281364189, Length=206, Percent_Identity=31.5533980582524, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI281364187, Length=206, Percent_Identity=31.5533980582524, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI281364185, Length=206, Percent_Identity=31.5533980582524, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI281364183, Length=206, Percent_Identity=31.5533980582524, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI62471759, Length=206, Percent_Identity=31.5533980582524, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI17975520, Length=206, Percent_Identity=31.5533980582524, Blast_Score=98, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_CORJK (Q4JV56)

Other databases:

- EMBL:   CR931997
- RefSeq:   YP_250919.1
- ProteinModelPortal:   Q4JV56
- SMR:   Q4JV56
- STRING:   Q4JV56
- GeneID:   3433059
- GenomeReviews:   CR931997_GR
- KEGG:   cjk:jk1137
- NMPDR:   fig|306537.3.peg.1176
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   YPAHMSK
- ProtClustDB:   PRK03287
- BioCyc:   CJEI306537:JK1137-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR012960
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR015225
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF08068 DKCLD; PF09142 TruB_C; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 33177; Mature: 33177

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: ACT_SITE 49-49

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQRSARSVLSRSGVVLVDKPSGPTSHDMVAKLRRIMGTRRIGHSGTLDPMATGLLVVGV
CCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCEEEEEE
ERGTKFLAHVVTHDKRYEATVRLGVATHTDDAQGDVLSTASPQDLQALTEQQVREAFAAQ
CHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHH
RGDIMQRPTSVSSIKIDGKRAHELVREGHDVVLPERPVTIFSLEVLDVVVDDATSCIDAR
CCCHHCCCCCCCEEEECCHHHHHHHHCCCCEECCCCCEEEEEHHHHHHHHHHHHHHEECE
ISVHCSSGTYIRAIARDVGEALGVGGHLTQLRRTSVGPFDVSEARTLEQLEEDPSLTLNL
EEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEH
DEAMVRCFDTREISESEGVDLSLGKWLKPVGNKGVRAAVTPSGQAIALVEEKGKRASSVF
HHHHHHHHCCCCCCCCCCCEEEHHHHHHHCCCCCCEEEECCCCCEEEEEECCCCCCCEEE
VARPAGMD
EECCCCCC
>Mature Secondary Structure
MNQRSARSVLSRSGVVLVDKPSGPTSHDMVAKLRRIMGTRRIGHSGTLDPMATGLLVVGV
CCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCEEEEEE
ERGTKFLAHVVTHDKRYEATVRLGVATHTDDAQGDVLSTASPQDLQALTEQQVREAFAAQ
CHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHH
RGDIMQRPTSVSSIKIDGKRAHELVREGHDVVLPERPVTIFSLEVLDVVVDDATSCIDAR
CCCHHCCCCCCCEEEECCHHHHHHHHCCCCEECCCCCEEEEEHHHHHHHHHHHHHHEECE
ISVHCSSGTYIRAIARDVGEALGVGGHLTQLRRTSVGPFDVSEARTLEQLEEDPSLTLNL
EEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEH
DEAMVRCFDTREISESEGVDLSLGKWLKPVGNKGVRAAVTPSGQAIALVEEKGKRASSVF
HHHHHHHHCCCCCCCCCCCEEEHHHHHHHCCCCCCEEEECCCCCEEEEEECCCCCCCEEE
VARPAGMD
EECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA