Click here to switch to the map view.

The map label for this gene is lepA

Identifier: 68535649

GI number: 68535649

Start: 697030

End: 698880

Strand: Direct

Name: lepA

Synonym: jk0577

Alternate gene names: 68535649

Gene position: 697030-698880 (Clockwise)

Preceding gene: 68535645

Following gene: 68535651

Centisome position: 28.31

GC content: 62.13

Gene sequence:

>1851_bases
ATGGCAGCCAAGCAGAAGAATTACGCCACAGAGACGTTTACGGACCCAGAGAGGATCCGTAACTTCTGCATCATTGCCCA
CATTGACCACGGCAAGTCCACTCTGGCGGACCGCATTCTGCAGATGTCGGGCGTGGTCGAGGACCGCGACATGCGCGACC
AGTACCTCGACAACATGGACATCGAACGCGAACGCGGCATCACCATTAAGGCGCAGAACGTGCGCCTGCCCTGGGTGCCG
AAGACCGGCGCGCACGCGGGCGAAGAGCTGGTGATGCACCTGATTGATACGCCCGGCCACGTGGACTTCACCTACGAGGT
GTCCCGCGCCCTCGAGGCGTGTGAGGGTTGCATCCTGCTAGTCGACGCGGCACAGGGTATCGAGGCGCAGACGCTGGCGA
ATCTGTACCTGGCGATGGAAAACGACCTGGAAATCATCCCGGTTCTGAACAAGATCGACCTGCCGGCAGCGGACCCGGAT
AAGTACGCCCTGGAGATTGCGCACATCATCGGCTGCGAGCCGGAGGACGTGCTGCGCGTATCCGGTAAGACGGGCGAAGG
CGTGTCCGAGTTGCTGGACAGGGTCTGTGAGCTCGTGCCCGCCCCGGTCGGCGACGCCGATGCGCCGGCCCGTGCCATGA
TCTTCGACAGTGTCTACGACATCTACCGCGGCGTGGTGACCTACGTGCGCATGATGGACGGCAAGCTGGAATCCCGCCAG
AAGATCCAGATGATGAGTACCGGCGCGACCCACGAAACCCTGGAAATCGGCGTGGTCTCCCCAGAGCCGACGAAGACCAA
GGGGCTCGGTGTGGGCGAGGTTGGCTACATCATCACCGGTGTGAAGGACGTCCGCCAGTCCAAGGTGGGCGATACGATCA
CTTGGGCCGTCAACGGTGCGGAAACCCCGTTGAAGGGCTACCAAGAACCGACCCCGATGGTGTACTCCGGCCTGTTCCCA
ATCAGTGCCGACCAGTATCCGGACCTGCGCGAGGCTATCGAGAAGCTGCAGCTCAACGATGCCTCCCTGACCTTCGAGCC
GGAGACTTCCGTGGCCCTGGGCTTCGGCTTCCGCTGTGGCTTCCTGGGCCTGCTGCATATGGAGATCACCCGCGCTCGCC
TGGAGCGCGAGTTCGACCTAGACCTGATCTCCACCGCGCCTTCCGTGGTGTACCGCGTGGTCAAGGAGGACGGCAGCGAG
GTAATGGTCCGCAATCCTTCGGACTGGCCGGGCGGCAAGATGCGCGAGATCTACGAGCCGATCGTGAAGATGACCGTGAT
CGTGCCGGCGGAGTTCCTGGGCGCGACGATGGAGCTATGCCAGTCCAAGCGCGGCCAGATGGGCGGCATGGACTACCTTT
CCGAGGATCGTGTGGAGCTGCGCTACACCATGCCGCTGGGCGAGATCATCTTCGACTTCTTCGATCAGCTGAAGTCCCGC
ACTAAGGGCTACGCTTCGCTGAACTACGAGGAGGCCGGCGAGCAGCTGGCCGACCTGGTCAAGGTGGACATCCTGCTGCA
GGGCGACCCGGTGGATGCGTTCAGCGCCATCGTTCACCGCGAGAATGCCCACTGGTACGGCAACAAGATGACCGTAAAGC
TCAAGGAACTCATCCCGCGCCAGCAGTTCGAAGTGCCGGTGCAGGCGGCCATCGGCTCCAAGATCATTGCCCGCGAGAAC
ATCCGCGCCCTGCGCAAGGACGTTCTTTCCAAGTGCTACGGCGGCGACGTTTCGCGTAAGCGCAAGCTGCTGGAAAAGCA
GAAGGAAGGCAAGAAGCGCATGAAGGCCATCGGTTCGGTCTCTGTTCCGCAGGAAGCCTTCGTCGCTGCTCTGTCTACTG
ACGCCGACTAG

Upstream 100 bases:

>100_bases
GAACCATGTGCTCACAGGAATAAGGTTCGCAGCTAAACCCCGTCTGCTACTTCTGTGCCGATGCATAGTAGGGTTACGCA
TAAGCGAAAGAGGAGGGCAA

Downstream 100 bases:

>100_bases
GTTTATTGACGCCGACTAGGCTCAGCCGAGCTGTCGTACCGCCGCGGCCAGGCGGTTGATCTCGCCTAGGGAAAGCTCCT
CGTCCGCGTGGGCGTTGCAG

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 616; Mature: 615

Protein sequence:

>616_residues
MAAKQKNYATETFTDPERIRNFCIIAHIDHGKSTLADRILQMSGVVEDRDMRDQYLDNMDIERERGITIKAQNVRLPWVP
KTGAHAGEELVMHLIDTPGHVDFTYEVSRALEACEGCILLVDAAQGIEAQTLANLYLAMENDLEIIPVLNKIDLPAADPD
KYALEIAHIIGCEPEDVLRVSGKTGEGVSELLDRVCELVPAPVGDADAPARAMIFDSVYDIYRGVVTYVRMMDGKLESRQ
KIQMMSTGATHETLEIGVVSPEPTKTKGLGVGEVGYIITGVKDVRQSKVGDTITWAVNGAETPLKGYQEPTPMVYSGLFP
ISADQYPDLREAIEKLQLNDASLTFEPETSVALGFGFRCGFLGLLHMEITRARLEREFDLDLISTAPSVVYRVVKEDGSE
VMVRNPSDWPGGKMREIYEPIVKMTVIVPAEFLGATMELCQSKRGQMGGMDYLSEDRVELRYTMPLGEIIFDFFDQLKSR
TKGYASLNYEEAGEQLADLVKVDILLQGDPVDAFSAIVHRENAHWYGNKMTVKLKELIPRQQFEVPVQAAIGSKIIAREN
IRALRKDVLSKCYGGDVSRKRKLLEKQKEGKKRMKAIGSVSVPQEAFVAALSTDAD

Sequences:

>Translated_616_residues
MAAKQKNYATETFTDPERIRNFCIIAHIDHGKSTLADRILQMSGVVEDRDMRDQYLDNMDIERERGITIKAQNVRLPWVP
KTGAHAGEELVMHLIDTPGHVDFTYEVSRALEACEGCILLVDAAQGIEAQTLANLYLAMENDLEIIPVLNKIDLPAADPD
KYALEIAHIIGCEPEDVLRVSGKTGEGVSELLDRVCELVPAPVGDADAPARAMIFDSVYDIYRGVVTYVRMMDGKLESRQ
KIQMMSTGATHETLEIGVVSPEPTKTKGLGVGEVGYIITGVKDVRQSKVGDTITWAVNGAETPLKGYQEPTPMVYSGLFP
ISADQYPDLREAIEKLQLNDASLTFEPETSVALGFGFRCGFLGLLHMEITRARLEREFDLDLISTAPSVVYRVVKEDGSE
VMVRNPSDWPGGKMREIYEPIVKMTVIVPAEFLGATMELCQSKRGQMGGMDYLSEDRVELRYTMPLGEIIFDFFDQLKSR
TKGYASLNYEEAGEQLADLVKVDILLQGDPVDAFSAIVHRENAHWYGNKMTVKLKELIPRQQFEVPVQAAIGSKIIAREN
IRALRKDVLSKCYGGDVSRKRKLLEKQKEGKKRMKAIGSVSVPQEAFVAALSTDAD
>Mature_615_residues
AAKQKNYATETFTDPERIRNFCIIAHIDHGKSTLADRILQMSGVVEDRDMRDQYLDNMDIERERGITIKAQNVRLPWVPK
TGAHAGEELVMHLIDTPGHVDFTYEVSRALEACEGCILLVDAAQGIEAQTLANLYLAMENDLEIIPVLNKIDLPAADPDK
YALEIAHIIGCEPEDVLRVSGKTGEGVSELLDRVCELVPAPVGDADAPARAMIFDSVYDIYRGVVTYVRMMDGKLESRQK
IQMMSTGATHETLEIGVVSPEPTKTKGLGVGEVGYIITGVKDVRQSKVGDTITWAVNGAETPLKGYQEPTPMVYSGLFPI
SADQYPDLREAIEKLQLNDASLTFEPETSVALGFGFRCGFLGLLHMEITRARLEREFDLDLISTAPSVVYRVVKEDGSEV
MVRNPSDWPGGKMREIYEPIVKMTVIVPAEFLGATMELCQSKRGQMGGMDYLSEDRVELRYTMPLGEIIFDFFDQLKSRT
KGYASLNYEEAGEQLADLVKVDILLQGDPVDAFSAIVHRENAHWYGNKMTVKLKELIPRQQFEVPVQAAIGSKIIARENI
RALRKDVLSKCYGGDVSRKRKLLEKQKEGKKRMKAIGSVSVPQEAFVAALSTDAD

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=608, Percent_Identity=45.7236842105263, Blast_Score=580, Evalue=1e-165,
Organism=Homo sapiens, GI94966754, Length=154, Percent_Identity=41.5584415584416, Blast_Score=120, Evalue=6e-27,
Organism=Homo sapiens, GI18390331, Length=151, Percent_Identity=35.0993377483444, Blast_Score=99, Evalue=2e-20,
Organism=Homo sapiens, GI310132016, Length=131, Percent_Identity=38.9312977099237, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI310110807, Length=131, Percent_Identity=38.9312977099237, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI310123363, Length=131, Percent_Identity=38.9312977099237, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI25306283, Length=151, Percent_Identity=41.7218543046358, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI25306287, Length=151, Percent_Identity=41.7218543046358, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI19923640, Length=151, Percent_Identity=41.7218543046358, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI4503483, Length=145, Percent_Identity=35.1724137931034, Blast_Score=91, Evalue=5e-18,
Organism=Homo sapiens, GI217272892, Length=152, Percent_Identity=31.5789473684211, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI217272894, Length=152, Percent_Identity=31.5789473684211, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI94966752, Length=69, Percent_Identity=49.2753623188406, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI34147630, Length=311, Percent_Identity=25.7234726688103, Blast_Score=70, Evalue=7e-12,
Organism=Homo sapiens, GI53729339, Length=224, Percent_Identity=29.0178571428571, Blast_Score=67, Evalue=7e-11,
Organism=Homo sapiens, GI53729337, Length=224, Percent_Identity=29.0178571428571, Blast_Score=67, Evalue=7e-11,
Organism=Escherichia coli, GI1788922, Length=601, Percent_Identity=53.4109816971714, Blast_Score=627, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=507, Percent_Identity=27.0216962524655, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1790835, Length=181, Percent_Identity=31.4917127071823, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789738, Length=160, Percent_Identity=35, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1789559, Length=288, Percent_Identity=27.4305555555556, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=620, Percent_Identity=41.2903225806452, Blast_Score=466, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI71988819, Length=138, Percent_Identity=39.1304347826087, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71988811, Length=138, Percent_Identity=39.1304347826087, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=152, Percent_Identity=40.1315789473684, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17556745, Length=144, Percent_Identity=36.1111111111111, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17552882, Length=165, Percent_Identity=29.6969696969697, Blast_Score=79, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=603, Percent_Identity=43.7810945273632, Blast_Score=514, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6322359, Length=117, Percent_Identity=42.7350427350427, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6323098, Length=148, Percent_Identity=36.4864864864865, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324707, Length=208, Percent_Identity=31.7307692307692, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320593, Length=208, Percent_Identity=31.7307692307692, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=146, Percent_Identity=40.4109589041096, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324761, Length=251, Percent_Identity=26.2948207171315, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI78706572, Length=604, Percent_Identity=45.0331125827815, Blast_Score=540, Evalue=1e-153,
Organism=Drosophila melanogaster, GI28574573, Length=138, Percent_Identity=42.0289855072464, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24582462, Length=149, Percent_Identity=36.9127516778523, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24585711, Length=147, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24585713, Length=147, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24585709, Length=147, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=5e-17,
Organism=Drosophila melanogaster, GI221458488, Length=160, Percent_Identity=34.375, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21357743, Length=194, Percent_Identity=30.9278350515464, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281363316, Length=304, Percent_Identity=25.9868421052632, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17864358, Length=304, Percent_Identity=25.9868421052632, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_CORJK (Q4JWS1)

Other databases:

- EMBL:   CR931997
- RefSeq:   YP_250354.1
- ProteinModelPortal:   Q4JWS1
- SMR:   Q4JWS1
- STRING:   Q4JWS1
- GeneID:   3432478
- GenomeReviews:   CR931997_GR
- KEGG:   cjk:jk0577
- NMPDR:   fig|306537.3.peg.694
- eggNOG:   COG0481
- HOGENOM:   HBG286375
- OMA:   YDSYRGV
- ProtClustDB:   PRK05433
- BioCyc:   CJEI306537:JK0577-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- SMART:   SM00838
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 68445; Mature: 68314

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKQKNYATETFTDPERIRNFCIIAHIDHGKSTLADRILQMSGVVEDRDMRDQYLDNMD
CCCCCCCCCCCCCCCHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
IERERGITIKAQNVRLPWVPKTGAHAGEELVMHLIDTPGHVDFTYEVSRALEACEGCILL
CHHHCCCEEEECCEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCEEEE
VDAAQGIEAQTLANLYLAMENDLEIIPVLNKIDLPAADPDKYALEIAHIIGCEPEDVLRV
EECCCCCCHHHHHHHHEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHEEE
SGKTGEGVSELLDRVCELVPAPVGDADAPARAMIFDSVYDIYRGVVTYVRMMDGKLESRQ
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
KIQMMSTGATHETLEIGVVSPEPTKTKGLGVGEVGYIITGVKDVRQSKVGDTITWAVNGA
HHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHCHHHHHHHHCCCEEEEEECCC
ETPLKGYQEPTPMVYSGLFPISADQYPDLREAIEKLQLNDASLTFEPETSVALGFGFRCG
CCCCCCCCCCCCHHEECCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCEEEECCHHHH
FLGLLHMEITRARLEREFDLDLISTAPSVVYRVVKEDGSEVMVRNPSDWPGGKMREIYEP
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
IVKMTVIVPAEFLGATMELCQSKRGQMGGMDYLSEDRVELRYTMPLGEIIFDFFDQLKSR
HHHHHHEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHH
TKGYASLNYEEAGEQLADLVKVDILLQGDPVDAFSAIVHRENAHWYGNKMTVKLKELIPR
CCCCEECCHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHCCCCCEECCEEEEEHHHHCCC
QQFEVPVQAAIGSKIIARENIRALRKDVLSKCYGGDVSRKRKLLEKQKEGKKRMKAIGSV
HHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
SVPQEAFVAALSTDAD
CCCHHHHHHHHCCCCC
>Mature Secondary Structure 
AAKQKNYATETFTDPERIRNFCIIAHIDHGKSTLADRILQMSGVVEDRDMRDQYLDNMD
CCCCCCCCCCCCCCHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
IERERGITIKAQNVRLPWVPKTGAHAGEELVMHLIDTPGHVDFTYEVSRALEACEGCILL
CHHHCCCEEEECCEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCEEEE
VDAAQGIEAQTLANLYLAMENDLEIIPVLNKIDLPAADPDKYALEIAHIIGCEPEDVLRV
EECCCCCCHHHHHHHHEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHEEE
SGKTGEGVSELLDRVCELVPAPVGDADAPARAMIFDSVYDIYRGVVTYVRMMDGKLESRQ
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
KIQMMSTGATHETLEIGVVSPEPTKTKGLGVGEVGYIITGVKDVRQSKVGDTITWAVNGA
HHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHCHHHHHHHHCCCEEEEEECCC
ETPLKGYQEPTPMVYSGLFPISADQYPDLREAIEKLQLNDASLTFEPETSVALGFGFRCG
CCCCCCCCCCCCHHEECCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCEEEECCHHHH
FLGLLHMEITRARLEREFDLDLISTAPSVVYRVVKEDGSEVMVRNPSDWPGGKMREIYEP
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
IVKMTVIVPAEFLGATMELCQSKRGQMGGMDYLSEDRVELRYTMPLGEIIFDFFDQLKSR
HHHHHHEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHH
TKGYASLNYEEAGEQLADLVKVDILLQGDPVDAFSAIVHRENAHWYGNKMTVKLKELIPR
CCCCEECCHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHCCCCCEECCEEEEEHHHHCCC
QQFEVPVQAAIGSKIIARENIRALRKDVLSKCYGGDVSRKRKLLEKQKEGKKRMKAIGSV
HHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
SVPQEAFVAALSTDAD
CCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA