Click here to switch to the map view.

The map label for this gene is folC [H]

Identifier: 68535623

GI number: 68535623

Start: 669127

End: 670665

Strand: Direct

Name: folC [H]

Synonym: jk0551

Alternate gene names: 68535623

Gene position: 669127-670665 (Clockwise)

Preceding gene: 68535622

Following gene: 68535624

Centisome position: 27.17

GC content: 67.32

Gene sequence:

>1539_bases
ATGAGCGAGGACAAGAGTGCCGCCGAAAAGGTCGGGGATAGCTACCCGGAAGAGGTTACGCTCGACGAGGCAGGCCTAGC
CCTGCCTATCGACGGTGTGGGTGCGGATGAGGCCGAGGTGGCGTCAGTACAAGAACGTCCCATTACGCCCGAAGACCTGG
CCGCGCTGGCGGAGGTCGACGCGGAGCTGAATCAGCGCTGGCCGGAGACGAAGATAGACCCGACGCTCGAGCGCGTCGAG
ATGCTGATGGACCTGCTCGGCCATCCGGAGCGTGCGTTCCCGGTGATCCACGTGGCGGGCACCAACGGCAAGACCTCCGT
GGTGCGGATGATCGAATCGCTGATGCGCGCCTTCCACCGTCGCACCGGGCGGACAACCAGCCCGCACCTGCAGATCGTCA
CCGAACGCATCGCCATCGACGGCAAGCCGCTACACCCACGCGACTACGTGGATACGTGGCGCGACATCCAGCCGATGGTG
GAAATGGTGGACGCGAAGAGCCAGGCCGAGGGTGGCCCGCAGATGAGCAAGTTCGAGGTGCTTGTGGCCATGGCCTACGC
GGCCTTCGCCGACGCGCCGGTGGACGTCGCCGTGGTGGAGACCGGCATGGGCGGGCGCTGGGACGCAACGAACGTGGTGA
ACTCTGACGTTGCCGTGATCACCCCGATCGGGCTGGATCACACCGACTACCTGGGCGACACGCTAGAGGAAATCGCGGGC
GAGAAGGCGGGAATCATCAAGGCCCGCTGGGATGCCGACGATCTGCTTACCCCGCCGGACAACGTGACCATCGTCGCTGA
GCAGGAGCCAGAGGCCATGCGAGTGCTGCTGGAAGAGGCTGTGGCGAAGGATTCCGCCGTCGCCCGTGCGGGCAGCGAGT
TCGGTGTAGTTTCCAGCCAGATTGCCGTTGGCGGACAGACCGTGACCATCCGCGGGCTGGCCGGTGAGTACACGGACATC
TTCCTTCCGCTCTCCGGCGAGCACCAGGCGCGCAACGCTGCCGTGGCCCTGGCGGCTGTGGAGGCGTTCTTTGGCGCCGG
AGCTGAGCGCCAGCTGGACGTGGATACGGTGCGGGAAGGCTTTGCCTCCGTTGCCTCGCCCGGCCGCCTGGAGCGCGTGC
GCTCCACCCCGAGCGTGTTTATCGACGCCTCGCACAACCCCCACGGGGCGAAAGCCCTGGCAGCGGCCATCGACCGCGAC
TTCGAGTTCCGTCGGCTGGTCGGCGTGGTCGGAGTGCTGGGGGACAAGGATGCCCGCGGCATCCTGGCCGCCCTGGAGCC
AGTGATGGACGAGATCGTGGTGACGGAGGTGAATTCCCCGCGCGCCCTGCCGGTGGACGTGCTGGCCGAGTACGCGCGGG
ATGCCTTCGGCGAGGAGCGCGTGCACGTGGCCGCGAACCTGCCAGGTGCCGTGGAGATCGCCACCCAACTGGCCGAGGAT
GACGAGGACGGCGTGCTGTCTGGCGCTGGCGTGCTGATCACCGGCTCGGTGGTGACGGCCGGGGAGGCCCGCACACTGTT
CGGCAAGGAACCGGCGTAG

Upstream 100 bases:

>100_bases
AGGCGCCGGAGAAGGTCGTCGAAGGAATCCGCGAACGCCAGCGCGTCGCACAGGAAGAGTTCGAGCGCATCACTGCCCGC
CTGGAAGGTCTGCCAAAGGC

Downstream 100 bases:

>100_bases
GCAGTCAGCGCGGGCGCCGGGACACTCGCCGGCGCTAGGAAAATTTTTTAAACGGAAGATCTACCAAGGAGCTGCAGAAT
CGTGGCACAGAACCCGCATA

Product: dihydrofolate synthase / folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MSEDKSAAEKVGDSYPEEVTLDEAGLALPIDGVGADEAEVASVQERPITPEDLAALAEVDAELNQRWPETKIDPTLERVE
MLMDLLGHPERAFPVIHVAGTNGKTSVVRMIESLMRAFHRRTGRTTSPHLQIVTERIAIDGKPLHPRDYVDTWRDIQPMV
EMVDAKSQAEGGPQMSKFEVLVAMAYAAFADAPVDVAVVETGMGGRWDATNVVNSDVAVITPIGLDHTDYLGDTLEEIAG
EKAGIIKARWDADDLLTPPDNVTIVAEQEPEAMRVLLEEAVAKDSAVARAGSEFGVVSSQIAVGGQTVTIRGLAGEYTDI
FLPLSGEHQARNAAVALAAVEAFFGAGAERQLDVDTVREGFASVASPGRLERVRSTPSVFIDASHNPHGAKALAAAIDRD
FEFRRLVGVVGVLGDKDARGILAALEPVMDEIVVTEVNSPRALPVDVLAEYARDAFGEERVHVAANLPGAVEIATQLAED
DEDGVLSGAGVLITGSVVTAGEARTLFGKEPA

Sequences:

>Translated_512_residues
MSEDKSAAEKVGDSYPEEVTLDEAGLALPIDGVGADEAEVASVQERPITPEDLAALAEVDAELNQRWPETKIDPTLERVE
MLMDLLGHPERAFPVIHVAGTNGKTSVVRMIESLMRAFHRRTGRTTSPHLQIVTERIAIDGKPLHPRDYVDTWRDIQPMV
EMVDAKSQAEGGPQMSKFEVLVAMAYAAFADAPVDVAVVETGMGGRWDATNVVNSDVAVITPIGLDHTDYLGDTLEEIAG
EKAGIIKARWDADDLLTPPDNVTIVAEQEPEAMRVLLEEAVAKDSAVARAGSEFGVVSSQIAVGGQTVTIRGLAGEYTDI
FLPLSGEHQARNAAVALAAVEAFFGAGAERQLDVDTVREGFASVASPGRLERVRSTPSVFIDASHNPHGAKALAAAIDRD
FEFRRLVGVVGVLGDKDARGILAALEPVMDEIVVTEVNSPRALPVDVLAEYARDAFGEERVHVAANLPGAVEIATQLAED
DEDGVLSGAGVLITGSVVTAGEARTLFGKEPA
>Mature_511_residues
SEDKSAAEKVGDSYPEEVTLDEAGLALPIDGVGADEAEVASVQERPITPEDLAALAEVDAELNQRWPETKIDPTLERVEM
LMDLLGHPERAFPVIHVAGTNGKTSVVRMIESLMRAFHRRTGRTTSPHLQIVTERIAIDGKPLHPRDYVDTWRDIQPMVE
MVDAKSQAEGGPQMSKFEVLVAMAYAAFADAPVDVAVVETGMGGRWDATNVVNSDVAVITPIGLDHTDYLGDTLEEIAGE
KAGIIKARWDADDLLTPPDNVTIVAEQEPEAMRVLLEEAVAKDSAVARAGSEFGVVSSQIAVGGQTVTIRGLAGEYTDIF
LPLSGEHQARNAAVALAAVEAFFGAGAERQLDVDTVREGFASVASPGRLERVRSTPSVFIDASHNPHGAKALAAAIDRDF
EFRRLVGVVGVLGDKDARGILAALEPVMDEIVVTEVNSPRALPVDVLAEYARDAFGEERVHVAANLPGAVEIATQLAEDD
EDGVLSGAGVLITGSVVTAGEARTLFGKEPA

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=383, Percent_Identity=29.242819843342, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI66932984, Length=383, Percent_Identity=29.242819843342, Blast_Score=133, Evalue=5e-31,
Organism=Escherichia coli, GI1788654, Length=403, Percent_Identity=33.0024813895782, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI71984923, Length=323, Percent_Identity=29.1021671826625, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17553148, Length=322, Percent_Identity=29.1925465838509, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17553150, Length=322, Percent_Identity=29.1925465838509, Blast_Score=108, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6323760, Length=386, Percent_Identity=30.5699481865285, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6324815, Length=193, Percent_Identity=31.6062176165803, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6322718, Length=174, Percent_Identity=26.4367816091954, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24641571, Length=284, Percent_Identity=30.9859154929577, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24581568, Length=179, Percent_Identity=34.0782122905028, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 54471; Mature: 54340

Theoretical pI: Translated: 4.22; Mature: 4.22

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDKSAAEKVGDSYPEEVTLDEAGLALPIDGVGADEAEVASVQERPITPEDLAALAEVD
CCCCHHHHHHHCCCCCCCEEECCCCCEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
AELNQRWPETKIDPTLERVEMLMDLLGHPERAFPVIHVAGTNGKTSVVRMIESLMRAFHR
HHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHCCEEEEECCCCHHHHHHHHHHHHHHHHH
RTGRTTSPHLQIVTERIAIDGKPLHPRDYVDTWRDIQPMVEMVDAKSQAEGGPQMSKFEV
HCCCCCCCHHHHEEHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LVAMAYAAFADAPVDVAVVETGMGGRWDATNVVNSDVAVITPIGLDHTDYLGDTLEEIAG
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCC
EKAGIIKARWDADDLLTPPDNVTIVAEQEPEAMRVLLEEAVAKDSAVARAGSEFGVVSSQ
CCCCEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
IAVGGQTVTIRGLAGEYTDIFLPLSGEHQARNAAVALAAVEAFFGAGAERQLDVDTVREG
EEECCEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
FASVASPGRLERVRSTPSVFIDASHNPHGAKALAAAIDRDFEFRRLVGVVGVLGDKDARG
HHHHCCCHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHH
ILAALEPVMDEIVVTEVNSPRALPVDVLAEYARDAFGEERVHVAANLPGAVEIATQLAED
HHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCC
DEDGVLSGAGVLITGSVVTAGEARTLFGKEPA
CCCCEEECCCEEEECCEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SEDKSAAEKVGDSYPEEVTLDEAGLALPIDGVGADEAEVASVQERPITPEDLAALAEVD
CCCHHHHHHHCCCCCCCEEECCCCCEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
AELNQRWPETKIDPTLERVEMLMDLLGHPERAFPVIHVAGTNGKTSVVRMIESLMRAFHR
HHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHCCEEEEECCCCHHHHHHHHHHHHHHHHH
RTGRTTSPHLQIVTERIAIDGKPLHPRDYVDTWRDIQPMVEMVDAKSQAEGGPQMSKFEV
HCCCCCCCHHHHEEHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LVAMAYAAFADAPVDVAVVETGMGGRWDATNVVNSDVAVITPIGLDHTDYLGDTLEEIAG
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCC
EKAGIIKARWDADDLLTPPDNVTIVAEQEPEAMRVLLEEAVAKDSAVARAGSEFGVVSSQ
CCCCEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
IAVGGQTVTIRGLAGEYTDIFLPLSGEHQARNAAVALAAVEAFFGAGAERQLDVDTVREG
EEECCEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
FASVASPGRLERVRSTPSVFIDASHNPHGAKALAAAIDRDFEFRRLVGVVGVLGDKDARG
HHHHCCCHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHH
ILAALEPVMDEIVVTEVNSPRALPVDVLAEYARDAFGEERVHVAANLPGAVEIATQLAED
HHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCC
DEDGVLSGAGVLITGSVVTAGEARTLFGKEPA
CCCCEEECCCEEEECCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]