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The map label for this gene is rph [H]

Identifier: 68535568

GI number: 68535568

Start: 608604

End: 609374

Strand: Direct

Name: rph [H]

Synonym: jk0498

Alternate gene names: 68535568

Gene position: 608604-609374 (Clockwise)

Preceding gene: 68535567

Following gene: 68535569

Centisome position: 24.71

GC content: 65.89

Gene sequence:

>771_bases
ATGACGGAAACCAACGTAGAAAATAACACCGACGCAAGCAGCAACTTCACCCGCGCCGACGGCCGTGCCACCGACGAGCT
ACGCCCGGTGCGTATCACCCGCGGTTTCACCAGCAACCCCGCAGGCAGCGTACTGGTGGAATTCGGCAATACCCGAGTCA
TGTGCACCGCCAGCGTGGAAGAAGGCGTGCCGCGCTTCAAGAAGGATTCCGGCGAGGGTTGGCTGACTGCCGAGTATGCG
ATGCTGCCGGCCTCCACCCACGAGCGCATGCCGCGCGAATCCATGAAGGGCAAGGTCAAGGGGCGCACCCAGGAGATCTC
CCGCCTCGTCGGCCGCGCGCTGCGCGCCGCCGTGGATCTGAAGGAACTGGGGGAGAACACCGTCAACATCGACTGCGACG
TTCTGCAGGCCGACGGCGGCACCCGCACCGCAGCCATCACCGGCGCCTACGTGGCTCTGGCTGATGCGCTGGCCGTGCTG
CAAGCTAGGGGCGTCGTACCAGGGCAACCGCTACGCGCACCCGTCGCCGCAGTGTCCGTGGGCATCATCGACGGCGTTCC
ATGCCTCGACCTGCCGTACGAGGAAGACTCGCGGGCGGACGTGGACATGAACGTGGCAATGACGGCCGAGGGGCGTTTCG
TGGAGATCCAGGGCACCGGCGAGAACGCGGAGTTCACTCGCGAGGAACTAAACACCCTGCTGGATCTGGCCGAAGGCGGC
CTGCGTGAGCTCATCGAAGCGCAGCGGGCGGCGCTGGCGCAGGAACTCTAG

Upstream 100 bases:

>100_bases
GTATGCGCCTATCTGTCGGCTAGAAGCCAGGGGATCTTCTAAGCTGCTGCCAAAGCATCAGCGGATAGCCTGTCCAAGGC
GGCTAGTAAACTTGAGCTCC

Downstream 100 bases:

>100_bases
ATCAGCAGAAGCAAACGCGACAAGAGAAGGGGAGTAGCACCGTGCGGGTACTGGTGGCCTCAAGGAATAAGAAGAAGCTA
GCGGAGCTGAACCGCATGTT

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTETNVENNTDASSNFTRADGRATDELRPVRITRGFTSNPAGSVLVEFGNTRVMCTASVEEGVPRFKKDSGEGWLTAEYA
MLPASTHERMPRESMKGKVKGRTQEISRLVGRALRAAVDLKELGENTVNIDCDVLQADGGTRTAAITGAYVALADALAVL
QARGVVPGQPLRAPVAAVSVGIIDGVPCLDLPYEEDSRADVDMNVAMTAEGRFVEIQGTGENAEFTREELNTLLDLAEGG
LRELIEAQRAALAQEL

Sequences:

>Translated_256_residues
MTETNVENNTDASSNFTRADGRATDELRPVRITRGFTSNPAGSVLVEFGNTRVMCTASVEEGVPRFKKDSGEGWLTAEYA
MLPASTHERMPRESMKGKVKGRTQEISRLVGRALRAAVDLKELGENTVNIDCDVLQADGGTRTAAITGAYVALADALAVL
QARGVVPGQPLRAPVAAVSVGIIDGVPCLDLPYEEDSRADVDMNVAMTAEGRFVEIQGTGENAEFTREELNTLLDLAEGG
LRELIEAQRAALAQEL
>Mature_255_residues
TETNVENNTDASSNFTRADGRATDELRPVRITRGFTSNPAGSVLVEFGNTRVMCTASVEEGVPRFKKDSGEGWLTAEYAM
LPASTHERMPRESMKGKVKGRTQEISRLVGRALRAAVDLKELGENTVNIDCDVLQADGGTRTAAITGAYVALADALAVLQ
ARGVVPGQPLRAPVAAVSVGIIDGVPCLDLPYEEDSRADVDMNVAMTAEGRFVEIQGTGENAEFTREELNTLLDLAEGGL
RELIEAQRAALAQEL

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=210, Percent_Identity=61.4285714285714, Blast_Score=262, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI71981632, Length=246, Percent_Identity=26.4227642276423, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24584046, Length=244, Percent_Identity=27.4590163934426, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 27423; Mature: 27292

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS00014 ER_TARGET ; PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETNVENNTDASSNFTRADGRATDELRPVRITRGFTSNPAGSVLVEFGNTRVMCTASVE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCEEEEEEECHH
EGVPRFKKDSGEGWLTAEYAMLPASTHERMPRESMKGKVKGRTQEISRLVGRALRAAVDL
HCCCCCCCCCCCCEEEEEEEECCCCHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
KELGENTVNIDCDVLQADGGTRTAAITGAYVALADALAVLQARGVVPGQPLRAPVAAVSV
HHHCCCEEEEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
GIIDGVPCLDLPYEEDSRADVDMNVAMTAEGRFVEIQGTGENAEFTREELNTLLDLAEGG
HHCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHCCH
LRELIEAQRAALAQEL
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TETNVENNTDASSNFTRADGRATDELRPVRITRGFTSNPAGSVLVEFGNTRVMCTASVE
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCEEEEEEECHH
EGVPRFKKDSGEGWLTAEYAMLPASTHERMPRESMKGKVKGRTQEISRLVGRALRAAVDL
HCCCCCCCCCCCCEEEEEEEECCCCHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
KELGENTVNIDCDVLQADGGTRTAAITGAYVALADALAVLQARGVVPGQPLRAPVAAVSV
HHHCCCEEEEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
GIIDGVPCLDLPYEEDSRADVDMNVAMTAEGRFVEIQGTGENAEFTREELNTLLDLAEGG
HHCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHCCH
LRELIEAQRAALAQEL
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]