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The map label for this gene is nei [H]

Identifier: 68535516

GI number: 68535516

Start: 536845

End: 537690

Strand: Direct

Name: nei [H]

Synonym: jk0446

Alternate gene names: 68535516

Gene position: 536845-537690 (Clockwise)

Preceding gene: 68535515

Following gene: 68535517

Centisome position: 21.8

GC content: 61.7

Gene sequence:

>846_bases
GTGCCTGAGGGTGACTCCGTACTCCAGCTTTCCAACCGCCTGCAATGGATGACGGGGCGGACGGTGCGGCATACGGACAT
CAGGGTTCCACGCTTTGCCACCGAGAGCTTCGACGGTGAAGAAGTCCGACGGGTGTGGCCGTATGGGAAGCACTTGTTTA
TGCACGTTGGCGACCGAGTGATCCACACACACCTGAAGATGGAAGGCGTGTGGGCGATTCACCGGGCTGGCCAACGCTGG
CGCAGGCCGGGCTATAGTGCGCGCATCGTGCTGCGCTTTAGCCCCCAGCACACCGGTGGCGAGGAGATAGAGCTAGTGGG
TCACGACCTCGGCTTCGTGCGGGTGTTCAACTTTGCGGATTATCCGCAGGTTATTGCGCACTTGGGGCCGGACATTCTGG
CGGAAGACTGGCAAGACGGCGGCAGGGAAGAGGCCATTGGGCGGATACTGCAACGGCCGGAAAGGACCCTCGGTGCCGCG
CTGCTGGATCAGAAGAACGTCGCGGGGATTGGCAACGAGTATCGCGCCGAGGTGATGTTCCTAGTGGGAATGCACCCGGA
GGTGAAGGTGGGGCAGGTGGGAGAAGTGGGCGTCACAAAAACAATAGACCTGGCGCGAAGAGTGATGTGGGATAACCGTC
TTGAGCCGCACCGAGTCTTCACAGGCGACCGTCGGGCCGGGCAAACTAACTGGGTGTTTGGCCGTGCGGGTAAGGCATGC
CGGCGGTGCGGGACGGCGATCCAGCAGTCAACGCTAGGAGGACGCTGGGCTGGTGGGGATCCGGATCTAGATTCGGCGGA
GTTGGAGCGGATTATCTGGTGGTGTCCGAACTGTCAGGAGCGGTGA

Upstream 100 bases:

>100_bases
GTGACCATAGAGAAGGTCAACGGTTCCTCGGTGATGGACATTGCTACGACAGATTGGGTGGCCGCAGGCGCACGCCTTAC
TCCGAAGGGGCTGAGCATCC

Downstream 100 bases:

>100_bases
GCTTCTTCGAGGTGGGGGAAGAGGTCGCTAGAGAGGCTGCTTCGCGGACTACTTGACAATCCGGTCAATAGCCCCAAAAA
GGGGTGGTTTCGTTCGGTGG

Product: putative endonuclease VIII

Products: NA

Alternate protein names: Putative DNA-(apurinic or apyrimidinic site) lyase Rv3297/MT3396; Putative AP lyase Rv3297/MT3396 [H]

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MPEGDSVLQLSNRLQWMTGRTVRHTDIRVPRFATESFDGEEVRRVWPYGKHLFMHVGDRVIHTHLKMEGVWAIHRAGQRW
RRPGYSARIVLRFSPQHTGGEEIELVGHDLGFVRVFNFADYPQVIAHLGPDILAEDWQDGGREEAIGRILQRPERTLGAA
LLDQKNVAGIGNEYRAEVMFLVGMHPEVKVGQVGEVGVTKTIDLARRVMWDNRLEPHRVFTGDRRAGQTNWVFGRAGKAC
RRCGTAIQQSTLGGRWAGGDPDLDSAELERIIWWCPNCQER

Sequences:

>Translated_281_residues
MPEGDSVLQLSNRLQWMTGRTVRHTDIRVPRFATESFDGEEVRRVWPYGKHLFMHVGDRVIHTHLKMEGVWAIHRAGQRW
RRPGYSARIVLRFSPQHTGGEEIELVGHDLGFVRVFNFADYPQVIAHLGPDILAEDWQDGGREEAIGRILQRPERTLGAA
LLDQKNVAGIGNEYRAEVMFLVGMHPEVKVGQVGEVGVTKTIDLARRVMWDNRLEPHRVFTGDRRAGQTNWVFGRAGKAC
RRCGTAIQQSTLGGRWAGGDPDLDSAELERIIWWCPNCQER
>Mature_280_residues
PEGDSVLQLSNRLQWMTGRTVRHTDIRVPRFATESFDGEEVRRVWPYGKHLFMHVGDRVIHTHLKMEGVWAIHRAGQRWR
RPGYSARIVLRFSPQHTGGEEIELVGHDLGFVRVFNFADYPQVIAHLGPDILAEDWQDGGREEAIGRILQRPERTLGAAL
LDQKNVAGIGNEYRAEVMFLVGMHPEVKVGQVGEVGVTKTIDLARRVMWDNRLEPHRVFTGDRRAGQTNWVFGRAGKACR
RCGTAIQQSTLGGRWAGGDPDLDSAELERIIWWCPNCQER

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA b

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR012319
- InterPro:   IPR010979
- InterPro:   IPR000214 [H]

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 31992; Mature: 31861

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS51066 ZF_FPG_2 ; PS51068 FPG_CAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEGDSVLQLSNRLQWMTGRTVRHTDIRVPRFATESFDGEEVRRVWPYGKHLFMHVGDRV
CCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHCCHH
IHTHLKMEGVWAIHRAGQRWRRPGYSARIVLRFSPQHTGGEEIELVGHDLGFVRVFNFAD
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEEECCCCEEEEECCCC
YPQVIAHLGPDILAEDWQDGGREEAIGRILQRPERTLGAALLDQKNVAGIGNEYRAEVMF
HHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCEEEEEE
LVGMHPEVKVGQVGEVGVTKTIDLARRVMWDNRLEPHRVFTGDRRAGQTNWVFGRAGKAC
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEECCCCHHH
RRCGTAIQQSTLGGRWAGGDPDLDSAELERIIWWCPNCQER
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHEEECCCCCCC
>Mature Secondary Structure 
PEGDSVLQLSNRLQWMTGRTVRHTDIRVPRFATESFDGEEVRRVWPYGKHLFMHVGDRV
CCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHCCHH
IHTHLKMEGVWAIHRAGQRWRRPGYSARIVLRFSPQHTGGEEIELVGHDLGFVRVFNFAD
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEEECCCCEEEEECCCC
YPQVIAHLGPDILAEDWQDGGREEAIGRILQRPERTLGAALLDQKNVAGIGNEYRAEVMF
HHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCEEEEEE
LVGMHPEVKVGQVGEVGVTKTIDLARRVMWDNRLEPHRVFTGDRRAGQTNWVFGRAGKAC
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEECCCCHHH
RRCGTAIQQSTLGGRWAGGDPDLDSAELERIIWWCPNCQER
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]