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The map label for this gene is purB [H]

Identifier: 68535414

GI number: 68535414

Start: 419117

End: 420547

Strand: Direct

Name: purB [H]

Synonym: jk0349

Alternate gene names: 68535414

Gene position: 419117-420547 (Clockwise)

Preceding gene: 68535413

Following gene: 68535415

Centisome position: 17.02

GC content: 64.85

Gene sequence:

>1431_bases
GTGCCTGCTAAGAAAAATATTTCCAACGTCCTTGCCAACCGCTACGCCTCCCCGGAGATGACCGCCATTTGGTCCCCGGA
GGACAAGATCATCATGGAGCGCAAGCTCTGGATCTCCGTCATGAAGGCCCAAGCTGACCTCGGGGTCGATGTCCCGGCCG
AGGCTATCGAGGCCTACGAGAAGGTCATCGAGAACGTAGACCTTGCATCCATCGCCGAACGCGAAAAAGTCACCCGCCAC
GACGTGAAGGCCCGCATCGAGGAATTCAGCGCGCTGGCCGGCCAGGAACACATCCACAAGGGCATGACCTCCCGCGACTT
GACGGAGAATGTGGAACAGCTGCAGATCTTCAGCTCCCTGCAGCTGGCCCGCGACAAGGCAGTTGCCGTCCTGGCCCGCA
TCGGCCGCCGCGCGGCGGAGAACCAGTCGCTGGTGATGGCTGGGCGCTCCCACAACGTGGCCGCCCAGGCAACGACCTTG
GGCAAGCGCTTTGCCTCCGCCGCCGATGAGCTGCTGGTGGGACTCGAGCGCATCGAGAACCTGCTGGGCCGCTACCCGCT
GCGTGGTATCAAGGGGCCGATGGGAACCAGCCAGGACATGCTGGACCTGGTGGGCGGCGACGAGTCCAAGCTGGCGGCGC
TGGAAAGCACCATCGCCGAGAACCTAGGCTTCTCCCGCGTATTCGATTCCGTTGGCCAGGTTTACCCGCGCAGCCTGGAC
ATGGACGCGCTGAGCGCCCTGGCTCAGATCGGCGCCGCCCTGTCCTCGCTGTCGATGACGATTCGTCTGATGGCGGGTAA
CGAGACCGTCACGGAGGGCTTCAAGGAAGGCCAGGTCGGTTCCTCCGCCATGCCGCACAAGATGAACGCCCGCTCCTGCG
AGCGCGTGGGCGGCATGCAGGTTCTGCTGCGCGGCTACCTAACCATGGCCGCCGACTTGGCCGGCGCGCAGTGGAACGAG
GGCGACGTGTTCTGCTCTGTGGTTCGTCGCGTTGCTCTTCCGGACGCCTTCTTCACCTTCGACGGCATGTGTGAGACCTT
CCTGACCGTCTTGGACGAGTTCGGGGTTTTCCCGGCGATGATCGACCGAGAGCTCGAGCGCTACCTGCCGTTCCTGGCCA
CCACGCGCATCCTGATGGCGGCCGTGCGCGCCGGGGTCGGCCGCGAGACGGCGCACGAGTTGATCAAGGAGCACGCCGTG
GCGGTGGCGCTGAACATGCGCGAGAACGGCGGCGACCAGGATCTGGTGCAGCGCCTCGCGGGCGACGACCGCTTCCCGCT
GGACGAGCCGCAGCTGGAGGAGGCGCTGGCGGATCGCCACGCATTCATCGGCGCGGCCGAATCTCAAGTATCCCGCGTTC
TGGCGCGCATCGAGGAAGTTAAGAATCGTCACCCAGAGGCCGCTGCTTACACGCCGGGCGAGATTCTTTAG

Upstream 100 bases:

>100_bases
TGATTCGCTCCGGCGACCTGGTGATTGCCGCGGGGCGGATCAAGGACCTGGATAAGTTTGCGGATTCCTAAAAGCCAGCG
GTACGCATAGACTCGGGGAC

Downstream 100 bases:

>100_bases
CTGCGTACTGGTGCAGCTACTAGTGCGCTTTTAGCTGCGCAAACGCCGAAGATTCGCACAGTTCCGGCCACAAATAGTCA
AATGCCTGGTACAGGGCTAC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MPAKKNISNVLANRYASPEMTAIWSPEDKIIMERKLWISVMKAQADLGVDVPAEAIEAYEKVIENVDLASIAEREKVTRH
DVKARIEEFSALAGQEHIHKGMTSRDLTENVEQLQIFSSLQLARDKAVAVLARIGRRAAENQSLVMAGRSHNVAAQATTL
GKRFASAADELLVGLERIENLLGRYPLRGIKGPMGTSQDMLDLVGGDESKLAALESTIAENLGFSRVFDSVGQVYPRSLD
MDALSALAQIGAALSSLSMTIRLMAGNETVTEGFKEGQVGSSAMPHKMNARSCERVGGMQVLLRGYLTMAADLAGAQWNE
GDVFCSVVRRVALPDAFFTFDGMCETFLTVLDEFGVFPAMIDRELERYLPFLATTRILMAAVRAGVGRETAHELIKEHAV
AVALNMRENGGDQDLVQRLAGDDRFPLDEPQLEEALADRHAFIGAAESQVSRVLARIEEVKNRHPEAAAYTPGEIL

Sequences:

>Translated_476_residues
MPAKKNISNVLANRYASPEMTAIWSPEDKIIMERKLWISVMKAQADLGVDVPAEAIEAYEKVIENVDLASIAEREKVTRH
DVKARIEEFSALAGQEHIHKGMTSRDLTENVEQLQIFSSLQLARDKAVAVLARIGRRAAENQSLVMAGRSHNVAAQATTL
GKRFASAADELLVGLERIENLLGRYPLRGIKGPMGTSQDMLDLVGGDESKLAALESTIAENLGFSRVFDSVGQVYPRSLD
MDALSALAQIGAALSSLSMTIRLMAGNETVTEGFKEGQVGSSAMPHKMNARSCERVGGMQVLLRGYLTMAADLAGAQWNE
GDVFCSVVRRVALPDAFFTFDGMCETFLTVLDEFGVFPAMIDRELERYLPFLATTRILMAAVRAGVGRETAHELIKEHAV
AVALNMRENGGDQDLVQRLAGDDRFPLDEPQLEEALADRHAFIGAAESQVSRVLARIEEVKNRHPEAAAYTPGEIL
>Mature_475_residues
PAKKNISNVLANRYASPEMTAIWSPEDKIIMERKLWISVMKAQADLGVDVPAEAIEAYEKVIENVDLASIAEREKVTRHD
VKARIEEFSALAGQEHIHKGMTSRDLTENVEQLQIFSSLQLARDKAVAVLARIGRRAAENQSLVMAGRSHNVAAQATTLG
KRFASAADELLVGLERIENLLGRYPLRGIKGPMGTSQDMLDLVGGDESKLAALESTIAENLGFSRVFDSVGQVYPRSLDM
DALSALAQIGAALSSLSMTIRLMAGNETVTEGFKEGQVGSSAMPHKMNARSCERVGGMQVLLRGYLTMAADLAGAQWNEG
DVFCSVVRRVALPDAFFTFDGMCETFLTVLDEFGVFPAMIDRELERYLPFLATTRILMAAVRAGVGRETAHELIKEHAVA
VALNMRENGGDQDLVQRLAGDDRFPLDEPQLEEALADRHAFIGAAESQVSRVLARIEEVKNRHPEAAAYTPGEIL

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=449, Percent_Identity=35.4120267260579, Blast_Score=263, Evalue=4e-70,
Organism=Homo sapiens, GI183227688, Length=392, Percent_Identity=35.2040816326531, Blast_Score=229, Evalue=4e-60,
Organism=Caenorhabditis elegans, GI17508577, Length=405, Percent_Identity=29.1358024691358, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI32564234, Length=352, Percent_Identity=30.1136363636364, Blast_Score=148, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6323391, Length=463, Percent_Identity=35.4211663066955, Blast_Score=264, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24647570, Length=462, Percent_Identity=32.034632034632, Blast_Score=224, Evalue=8e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 52011; Mature: 51880

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAKKNISNVLANRYASPEMTAIWSPEDKIIMERKLWISVMKAQADLGVDVPAEAIEAYE
CCCHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
KVIENVDLASIAEREKVTRHDVKARIEEFSALAGQEHIHKGMTSRDLTENVEQLQIFSSL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QLARDKAVAVLARIGRRAAENQSLVMAGRSHNVAAQATTLGKRFASAADELLVGLERIEN
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGRYPLRGIKGPMGTSQDMLDLVGGDESKLAALESTIAENLGFSRVFDSVGQVYPRSLD
HHHCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCC
MDALSALAQIGAALSSLSMTIRLMAGNETVTEGFKEGQVGSSAMPHKMNARSCERVGGMQ
HHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHCHHCCCCCCCCCCCCCCHHHHHHHHHHH
VLLRGYLTMAADLAGAQWNEGDVFCSVVRRVALPDAFFTFDGMCETFLTVLDEFGVFPAM
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHH
IDRELERYLPFLATTRILMAAVRAGVGRETAHELIKEHAVAVALNMRENGGDQDLVQRLA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHC
GDDRFPLDEPQLEEALADRHAFIGAAESQVSRVLARIEEVKNRHPEAAAYTPGEIL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
PAKKNISNVLANRYASPEMTAIWSPEDKIIMERKLWISVMKAQADLGVDVPAEAIEAYE
CCHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
KVIENVDLASIAEREKVTRHDVKARIEEFSALAGQEHIHKGMTSRDLTENVEQLQIFSSL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QLARDKAVAVLARIGRRAAENQSLVMAGRSHNVAAQATTLGKRFASAADELLVGLERIEN
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGRYPLRGIKGPMGTSQDMLDLVGGDESKLAALESTIAENLGFSRVFDSVGQVYPRSLD
HHHCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCC
MDALSALAQIGAALSSLSMTIRLMAGNETVTEGFKEGQVGSSAMPHKMNARSCERVGGMQ
HHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHCHHCCCCCCCCCCCCCCHHHHHHHHHHH
VLLRGYLTMAADLAGAQWNEGDVFCSVVRRVALPDAFFTFDGMCETFLTVLDEFGVFPAM
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHH
IDRELERYLPFLATTRILMAAVRAGVGRETAHELIKEHAVAVALNMRENGGDQDLVQRLA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHC
GDDRFPLDEPQLEEALADRHAFIGAAESQVSRVLARIEEVKNRHPEAAAYTPGEIL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]