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The map label for this gene is yjfH [C]

Identifier: 68535387

GI number: 68535387

Start: 391456

End: 392412

Strand: Direct

Name: yjfH [C]

Synonym: jk0322

Alternate gene names: 68535387

Gene position: 391456-392412 (Clockwise)

Preceding gene: 68535386

Following gene: 68535389

Centisome position: 15.9

GC content: 67.82

Gene sequence:

>957_bases
GTGGCGGGCAACACCCAGCGCAGCGGAGGCGTGCGCAAGAAGAACAAGAAGGGCGCAACCAAGGGCTCCGGTGGCCAGGG
GCGCCGCAGCCTGCGGGGCAAGGGGGCCACGCCGAAGGCGGAGGATCGCGTCTACCACGCCGCATACAAGCGCAAGCAGG
CCGCCAAGCGCCGGGCATCTGGTGCGCACGACCGCTTCGCAAAGGACGACAACCAGGTGGAGCTGGTTGTTGGCCGCAAC
CCGGTGATCGAGTGCCTGCACGCCAAGGTCCCCGCCACCGCGCTGTACGTTGCGGAGGGCACCAAGAACGACGAGCGCCT
GGCAGAGGCCGTCCATACCTGCGCCGACCGAGGCATCTCCGTGCTGGAGGTTTCCCGCGCAGAGCTGGACCGCATGACCG
GCAACGGCATGCACCAGGGCATTGGCCTGCAGATCCCGCCTTACCAGTACACGCAGGTGGAGGACCTGATTCAGCAGGCC
GCCTCCGCTCCTCAGCCCGGTCTGGTCGTCGCGCTGGATAACATCACCGACCCGCGTAACCTCGGTGCGGTCATCCGCTC
CGTAGCGGCCTTCGGCGGCCACGGCGTGATTATCCCGGAGCGCCGCAGCGCCTCCGTCACCGCTGTTGCTTGGCGTACCT
CCGCGGGCACGGCTGCGCGCCTGCCGGTGGCGAAGGCCACCAACATGGTGCGTTCCCTGAAGCAGTTCCAGCAGGCCGGC
TACCAGGTTGTAGGCCTGGACGCCGGTGGCGACCACACCCTCGATACCTACGACGGCACTACCCCGACCGTCATCGTCGT
CGGCTCGGAGGGCAAGGGGCTGTCCCGCCTGGTTTCGGAGACCTGCGACACGATCATGTCGATCCCCATGGCCAAGTGGG
TGGAATCCCTCAATGCGTCGGTCGCTGCGGGCGTGGTGCTCAGCGAGTTCGCCCGCCAGCGTCGCGCTGCTAAGTGA

Upstream 100 bases:

>100_bases
CTTTGCTGTCCATGTACTCGTGAGCCTGGTTCCAGTTTCGCGTGCCGGGAAGTAACATACACAAACGCACGTAGTCGACC
AGGTAAGAGGGGCAATCACA

Downstream 100 bases:

>100_bases
CGCCCCCTCGTCTTCGCCTCTGCGGATTCCTCAGCAGGGGCTTCTAGTTTCTAAGGCTTAAGTCCTCGAATGTATGTCTC
CACGAGCCAGGGCTGGTAAT

Product: putative rRNA methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MAGNTQRSGGVRKKNKKGATKGSGGQGRRSLRGKGATPKAEDRVYHAAYKRKQAAKRRASGAHDRFAKDDNQVELVVGRN
PVIECLHAKVPATALYVAEGTKNDERLAEAVHTCADRGISVLEVSRAELDRMTGNGMHQGIGLQIPPYQYTQVEDLIQQA
ASAPQPGLVVALDNITDPRNLGAVIRSVAAFGGHGVIIPERRSASVTAVAWRTSAGTAARLPVAKATNMVRSLKQFQQAG
YQVVGLDAGGDHTLDTYDGTTPTVIVVGSEGKGLSRLVSETCDTIMSIPMAKWVESLNASVAAGVVLSEFARQRRAAK

Sequences:

>Translated_318_residues
MAGNTQRSGGVRKKNKKGATKGSGGQGRRSLRGKGATPKAEDRVYHAAYKRKQAAKRRASGAHDRFAKDDNQVELVVGRN
PVIECLHAKVPATALYVAEGTKNDERLAEAVHTCADRGISVLEVSRAELDRMTGNGMHQGIGLQIPPYQYTQVEDLIQQA
ASAPQPGLVVALDNITDPRNLGAVIRSVAAFGGHGVIIPERRSASVTAVAWRTSAGTAARLPVAKATNMVRSLKQFQQAG
YQVVGLDAGGDHTLDTYDGTTPTVIVVGSEGKGLSRLVSETCDTIMSIPMAKWVESLNASVAAGVVLSEFARQRRAAK
>Mature_317_residues
AGNTQRSGGVRKKNKKGATKGSGGQGRRSLRGKGATPKAEDRVYHAAYKRKQAAKRRASGAHDRFAKDDNQVELVVGRNP
VIECLHAKVPATALYVAEGTKNDERLAEAVHTCADRGISVLEVSRAELDRMTGNGMHQGIGLQIPPYQYTQVEDLIQQAA
SAPQPGLVVALDNITDPRNLGAVIRSVAAFGGHGVIIPERRSASVTAVAWRTSAGTAARLPVAKATNMVRSLKQFQQAGY
QVVGLDAGGDHTLDTYDGTTPTVIVVGSEGKGLSRLVSETCDTIMSIPMAKWVESLNASVAAGVVLSEFARQRRAAK

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=273, Percent_Identity=29.6703296703297, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1790623, Length=220, Percent_Identity=39.0909090909091, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1788935, Length=211, Percent_Identity=32.2274881516588, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1790083, Length=175, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17555908, Length=263, Percent_Identity=29.6577946768061, Blast_Score=97, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 33747; Mature: 33616

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGNTQRSGGVRKKNKKGATKGSGGQGRRSLRGKGATPKAEDRVYHAAYKRKQAAKRRAS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
GAHDRFAKDDNQVELVVGRNPVIECLHAKVPATALYVAEGTKNDERLAEAVHTCADRGIS
CCHHHHCCCCCCEEEEECCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCE
VLEVSRAELDRMTGNGMHQGIGLQIPPYQYTQVEDLIQQAASAPQPGLVVALDNITDPRN
EEEHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHH
LGAVIRSVAAFGGHGVIIPERRSASVTAVAWRTSAGTAARLPVAKATNMVRSLKQFQQAG
HHHHHHHHHHHCCCEEEEECCCCCCEEEEEEECCCCCCHHCCHHHHHHHHHHHHHHHHCC
YQVVGLDAGGDHTLDTYDGTTPTVIVVGSEGKGLSRLVSETCDTIMSIPMAKWVESLNAS
CEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCHHHHHHHHHCCH
VAAGVVLSEFARQRRAAK
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AGNTQRSGGVRKKNKKGATKGSGGQGRRSLRGKGATPKAEDRVYHAAYKRKQAAKRRAS
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
GAHDRFAKDDNQVELVVGRNPVIECLHAKVPATALYVAEGTKNDERLAEAVHTCADRGIS
CCHHHHCCCCCCEEEEECCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCE
VLEVSRAELDRMTGNGMHQGIGLQIPPYQYTQVEDLIQQAASAPQPGLVVALDNITDPRN
EEEHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHH
LGAVIRSVAAFGGHGVIIPERRSASVTAVAWRTSAGTAARLPVAKATNMVRSLKQFQQAG
HHHHHHHHHHHCCCEEEEECCCCCCEEEEEEECCCCCCHHCCHHHHHHHHHHHHHHHHCC
YQVVGLDAGGDHTLDTYDGTTPTVIVVGSEGKGLSRLVSETCDTIMSIPMAKWVESLNAS
CEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCHHHHHHHHHCCH
VAAGVVLSEFARQRRAAK
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA