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The map label for this gene is fadE4 [H]

Identifier: 68535361

GI number: 68535361

Start: 360703

End: 361911

Strand: Direct

Name: fadE4 [H]

Synonym: jk0296

Alternate gene names: 68535361

Gene position: 360703-361911 (Clockwise)

Preceding gene: 68535360

Following gene: 68535364

Centisome position: 14.65

GC content: 64.1

Gene sequence:

>1209_bases
ATGATTAATCTGGAACTCCCCAAGCGTTTGAAGGCGGGCCAGAACCAAGCGCACCAGGCCGCCGCTGAAATTTTCCGCCC
CATCTCCCGCAAGTACGACCTCGCGGAGCATGAGCGCCCCGTCGAGCTGGACACCATGGCCAGCCTGGTCGAAGGCATGG
CAGATGCCGGCCAGGCAATGGCTGGTGCCTCCGGTGGCCGCGGCGATGACAAGAAGAAGGCTGAGGGCGTCAAGAATGGC
GGCAACATGGCCTCCATCCTCAACGTCATCGAGACCTGCTGGGGCGACGTGGGACTCACCCTGTCCATCCCCTACCAGGG
TCTGGGCAACGCAGCCGTAGCAGCCGTCGCCGATGACGAGCAGCTCGAGCGCTTCGGCAAGATCTGGGCCGCTATGGCCA
TCACCGAGCCGCAGTTCGGCTCCGACTCCGCAGCCGTCGCCGCCACCGCCAAGCTGGATGGCGACGAGTACGTGCTGAAC
GGTGAGAAGATCTTCGTCACCGCCGGTGAACGCTGCACCCACGTTGTCGTCTGGGCATCCGTCGACAAATCCGCAGGCCG
CGCGGCCATCAAGTCCTTCGTCGTTCCGCGCGACACCCCGGGCTTCGAACTGGTCCGCCTGGAGCACAAGCTGGGCATCC
GCTCCTCCGACACCGCTCACTTCATCCTGGACAACGTCCGCATCCCGAAGGAGAACCTGCTGGGCTCTCCGGAGGTGGAC
ACCAAGAAGGGCTTCGGTGGCGTGATGGCCACCTTCGACAACACCCGCCCGATGGTTGCCGCCATGGCCGTTGGTGTTGC
CCGCGCTTCCCTCGAGAAGCTGCGCGAGATCCTCACCGACGCAGGTGTAGAGATCGATTATGACGCCCCCGCGTGGAACC
AGCCCGCAGCTGCCAGCGAGTACATCCGCCTGGAGAGCGACTGGGAAGCTGCATACCTGATGACCCTGCGCGCAGGCTGG
ATGGCTGACAACAAGCAGCCGAACAGCAAGGAGGCTTCCGAGTGCAAGGCGAAGGCCGGCCGTATGGCTACCGACCTGAC
CCTGCGCGCGGTTGAGATCGCCGGCGCTTATGGCTACTCCGAGCGTGACCTGCTGGAGAAGTGGGCCCGCGACTCCAAGA
TCCTGGACATCTTCGAGGGCACCCAGCAGATTCAGCAGCTGGTTGTTGCCCGCCGCGAGCTGGGTCTTTCCTCCAAGGAG
CTCAAGTAG

Upstream 100 bases:

>100_bases
ATGCGCGCCATCGGCATCGCCGAGGGCGTAGTCGTCGTCTAACCCCGCCACAATTCGAATCCATATCGAATAAGCACAAG
GAACTTCAAGGAACCACATC

Downstream 100 bases:

>100_bases
TAGCTGGCTGCGCGAGAGCACGGTCAGCGGCTACGTTGCGCGCTACCGCTCGCGCCTGTGCTTAGCTGCCTAGCGTCGAG
CCGCGCGCTCGCGACTAGCT

Product: acyl-CoA dehydrogenase

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MINLELPKRLKAGQNQAHQAAAEIFRPISRKYDLAEHERPVELDTMASLVEGMADAGQAMAGASGGRGDDKKKAEGVKNG
GNMASILNVIETCWGDVGLTLSIPYQGLGNAAVAAVADDEQLERFGKIWAAMAITEPQFGSDSAAVAATAKLDGDEYVLN
GEKIFVTAGERCTHVVVWASVDKSAGRAAIKSFVVPRDTPGFELVRLEHKLGIRSSDTAHFILDNVRIPKENLLGSPEVD
TKKGFGGVMATFDNTRPMVAAMAVGVARASLEKLREILTDAGVEIDYDAPAWNQPAAASEYIRLESDWEAAYLMTLRAGW
MADNKQPNSKEASECKAKAGRMATDLTLRAVEIAGAYGYSERDLLEKWARDSKILDIFEGTQQIQQLVVARRELGLSSKE
LK

Sequences:

>Translated_402_residues
MINLELPKRLKAGQNQAHQAAAEIFRPISRKYDLAEHERPVELDTMASLVEGMADAGQAMAGASGGRGDDKKKAEGVKNG
GNMASILNVIETCWGDVGLTLSIPYQGLGNAAVAAVADDEQLERFGKIWAAMAITEPQFGSDSAAVAATAKLDGDEYVLN
GEKIFVTAGERCTHVVVWASVDKSAGRAAIKSFVVPRDTPGFELVRLEHKLGIRSSDTAHFILDNVRIPKENLLGSPEVD
TKKGFGGVMATFDNTRPMVAAMAVGVARASLEKLREILTDAGVEIDYDAPAWNQPAAASEYIRLESDWEAAYLMTLRAGW
MADNKQPNSKEASECKAKAGRMATDLTLRAVEIAGAYGYSERDLLEKWARDSKILDIFEGTQQIQQLVVARRELGLSSKE
LK
>Mature_402_residues
MINLELPKRLKAGQNQAHQAAAEIFRPISRKYDLAEHERPVELDTMASLVEGMADAGQAMAGASGGRGDDKKKAEGVKNG
GNMASILNVIETCWGDVGLTLSIPYQGLGNAAVAAVADDEQLERFGKIWAAMAITEPQFGSDSAAVAATAKLDGDEYVLN
GEKIFVTAGERCTHVVVWASVDKSAGRAAIKSFVVPRDTPGFELVRLEHKLGIRSSDTAHFILDNVRIPKENLLGSPEVD
TKKGFGGVMATFDNTRPMVAAMAVGVARASLEKLREILTDAGVEIDYDAPAWNQPAAASEYIRLESDWEAAYLMTLRAGW
MADNKQPNSKEASECKAKAGRMATDLTLRAVEIAGAYGYSERDLLEKWARDSKILDIFEGTQQIQQLVVARRELGLSSKE
LK

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: COG1960

COG function: function code I; Acyl-CoA dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4557233, Length=265, Percent_Identity=37.3584905660377, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI4557231, Length=327, Percent_Identity=33.3333333333333, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI187960098, Length=327, Percent_Identity=33.3333333333333, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI4501859, Length=262, Percent_Identity=30.9160305343511, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI21361497, Length=336, Percent_Identity=27.9761904761905, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI7656849, Length=316, Percent_Identity=27.5316455696203, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI226958414, Length=274, Percent_Identity=27.7372262773723, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI226958412, Length=274, Percent_Identity=27.7372262773723, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI4501857, Length=302, Percent_Identity=25.8278145695364, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI76496475, Length=400, Percent_Identity=27.5, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI4557235, Length=400, Percent_Identity=27.5, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI4503943, Length=294, Percent_Identity=22.4489795918367, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI48976061, Length=277, Percent_Identity=25.2707581227437, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI211971008, Length=277, Percent_Identity=25.2707581227437, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI7669494, Length=278, Percent_Identity=21.9424460431655, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1786223, Length=265, Percent_Identity=24.1509433962264, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI87081958, Length=265, Percent_Identity=25.6603773584906, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17570075, Length=314, Percent_Identity=35.031847133758, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17534899, Length=319, Percent_Identity=32.9153605015674, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17569725, Length=311, Percent_Identity=33.1189710610932, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17508101, Length=261, Percent_Identity=30.6513409961686, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17506239, Length=266, Percent_Identity=30.0751879699248, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17538396, Length=271, Percent_Identity=25.830258302583, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71990804, Length=246, Percent_Identity=28.4552845528455, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71985184, Length=326, Percent_Identity=26.3803680981595, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17505929, Length=303, Percent_Identity=26.4026402640264, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17533517, Length=277, Percent_Identity=26.7148014440433, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17551932, Length=294, Percent_Identity=24.8299319727891, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17534353, Length=279, Percent_Identity=24.0143369175627, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71985192, Length=214, Percent_Identity=29.9065420560748, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI32563615, Length=144, Percent_Identity=31.9444444444444, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71982178, Length=143, Percent_Identity=30.0699300699301, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356377, Length=275, Percent_Identity=36, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24646207, Length=321, Percent_Identity=31.4641744548287, Blast_Score=161, Evalue=8e-40,
Organism=Drosophila melanogaster, GI24660351, Length=324, Percent_Identity=35.4938271604938, Blast_Score=155, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24666513, Length=261, Percent_Identity=30.6513409961686, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI281363737, Length=340, Percent_Identity=29.1176470588235, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI19920834, Length=306, Percent_Identity=26.797385620915, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21355753, Length=290, Percent_Identity=26.2068965517241, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 43485; Mature: 43485

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINLELPKRLKAGQNQAHQAAAEIFRPISRKYDLAEHERPVELDTMASLVEGMADAGQAM
CCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHH
AGASGGRGDDKKKAEGVKNGGNMASILNVIETCWGDVGLTLSIPYQGLGNAAVAAVADDE
HCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCHH
QLERFGKIWAAMAITEPQFGSDSAAVAATAKLDGDEYVLNGEKIFVTAGERCTHVVVWAS
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCEEEECCCEEEEECCCCEEEEEEEEE
VDKSAGRAAIKSFVVPRDTPGFELVRLEHKLGIRSSDTAHFILDNVRIPKENLLGSPEVD
CCCCCHHHHHHHHCCCCCCCCCEEEEEHHHCCCCCCCCCEEEECCCCCCHHHCCCCCCCC
TKKGFGGVMATFDNTRPMVAAMAVGVARASLEKLREILTDAGVEIDYDAPAWNQPAAASE
CCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHH
YIRLESDWEAAYLMTLRAGWMADNKQPNSKEASECKAKAGRMATDLTLRAVEIAGAYGYS
EEEECCCCCCEEHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHEEEEEEECCCCCCC
ERDLLEKWARDSKILDIFEGTQQIQQLVVARRELGLSSKELK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MINLELPKRLKAGQNQAHQAAAEIFRPISRKYDLAEHERPVELDTMASLVEGMADAGQAM
CCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHH
AGASGGRGDDKKKAEGVKNGGNMASILNVIETCWGDVGLTLSIPYQGLGNAAVAAVADDE
HCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCHH
QLERFGKIWAAMAITEPQFGSDSAAVAATAKLDGDEYVLNGEKIFVTAGERCTHVVVWAS
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCEEEECCCEEEEECCCCEEEEEEEEE
VDKSAGRAAIKSFVVPRDTPGFELVRLEHKLGIRSSDTAHFILDNVRIPKENLLGSPEVD
CCCCCHHHHHHHHCCCCCCCCCEEEEEHHHCCCCCCCCCEEEECCCCCCHHHCCCCCCCC
TKKGFGGVMATFDNTRPMVAAMAVGVARASLEKLREILTDAGVEIDYDAPAWNQPAAASE
CCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHH
YIRLESDWEAAYLMTLRAGWMADNKQPNSKEASECKAKAGRMATDLTLRAVEIAGAYGYS
EEEECCCCCCEEHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHEEEEEEECCCCCCC
ERDLLEKWARDSKILDIFEGTQQIQQLVVARRELGLSSKELK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]