Click here to switch to the map view.

The map label for this gene is folE [H]

Identifier: 68535344

GI number: 68535344

Start: 343771

End: 344367

Strand: Direct

Name: folE [H]

Synonym: jk0279

Alternate gene names: 68535344

Gene position: 343771-344367 (Clockwise)

Preceding gene: 68535343

Following gene: 68535345

Centisome position: 13.96

GC content: 65.49

Gene sequence:

>597_bases
GTGAATAACCCACAGCAGCCGCTCGACCCCGGCTCCTTCGATCAGGAGCGGGCAGAGGCGGCCATCCGTGAACTCCTTTT
CGCCATTGGCGAAGACCCCGACCGTGAGGGTCTGCAGGAAACCCCGGCCCGTGTGGCCCGCGCCTACCGCGAGACGTGCG
CCGGGCTATACGAGGATCCCACGGAGGTTCTCAACAAGACCTTCAGCGAGGATCACCGCGAGCTGGTGCTCGTGCGCGAC
ATCACCTTCTACTCCATGTGTGAGCACCACCTGGTGCCCTTCTTCGGCAAGGCGCACATCGGATACATCCCCGGTGAATC
CGGCAAGGTCACAGGCCTGTCCAAACTGGCCCGCCTGATCGACGGTCTGGCGAAGCGCCCGCAGGTCCAGGAACGCCTGA
CCTCGCAGGTGGCCGACGCGCTGGTGGACAAGCTGGACCCCTCTGCAGTGATCGTTGTTCTGGAGGCCGAGCACCTGTGC
ATGGCGATGCGCGGCATCCGCAAGCCCGGCGCCAACACGGTGACCTCTGCGGTTCGTGGCGGATTCCAGAAGAACGCGGC
CTCCCGCGCAGAGGCGATGGCGCTGATCCGGCCGTGA

Upstream 100 bases:

>100_bases
GTCGCGGCGAGATGCCGGAGCAGACGTTCCCTTGGGATAAGCACAACGGTGGCTACAACGGCAACGAGTCCGGTGGCCAG
CACAGGCAGGAAGACAAGTA

Downstream 100 bases:

>100_bases
CGGGCTCTGCTGTGACTAGCACCAACGCGAACGACCCCCAGCGCACCCTCGTGATGGGCATCCTCAACGTCACCGAGGAT
TCCTTCTCCGACGGCGGGGC

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MNNPQQPLDPGSFDQERAEAAIRELLFAIGEDPDREGLQETPARVARAYRETCAGLYEDPTEVLNKTFSEDHRELVLVRD
ITFYSMCEHHLVPFFGKAHIGYIPGESGKVTGLSKLARLIDGLAKRPQVQERLTSQVADALVDKLDPSAVIVVLEAEHLC
MAMRGIRKPGANTVTSAVRGGFQKNAASRAEAMALIRP

Sequences:

>Translated_198_residues
MNNPQQPLDPGSFDQERAEAAIRELLFAIGEDPDREGLQETPARVARAYRETCAGLYEDPTEVLNKTFSEDHRELVLVRD
ITFYSMCEHHLVPFFGKAHIGYIPGESGKVTGLSKLARLIDGLAKRPQVQERLTSQVADALVDKLDPSAVIVVLEAEHLC
MAMRGIRKPGANTVTSAVRGGFQKNAASRAEAMALIRP
>Mature_198_residues
MNNPQQPLDPGSFDQERAEAAIRELLFAIGEDPDREGLQETPARVARAYRETCAGLYEDPTEVLNKTFSEDHRELVLVRD
ITFYSMCEHHLVPFFGKAHIGYIPGESGKVTGLSKLARLIDGLAKRPQVQERLTSQVADALVDKLDPSAVIVVLEAEHLC
MAMRGIRKPGANTVTSAVRGGFQKNAASRAEAMALIRP

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=179, Percent_Identity=49.7206703910614, Blast_Score=178, Evalue=3e-45,
Organism=Homo sapiens, GI4503949, Length=179, Percent_Identity=49.7206703910614, Blast_Score=178, Evalue=3e-45,
Organism=Homo sapiens, GI66932970, Length=138, Percent_Identity=51.4492753623188, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI66932972, Length=138, Percent_Identity=51.4492753623188, Blast_Score=139, Evalue=2e-33,
Organism=Escherichia coli, GI1788476, Length=204, Percent_Identity=37.7450980392157, Blast_Score=110, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17560486, Length=177, Percent_Identity=49.1525423728814, Blast_Score=178, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321706, Length=183, Percent_Identity=52.4590163934426, Blast_Score=184, Evalue=9e-48,
Organism=Drosophila melanogaster, GI24656782, Length=178, Percent_Identity=47.1910112359551, Blast_Score=161, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24656796, Length=177, Percent_Identity=47.4576271186441, Blast_Score=160, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24656791, Length=177, Percent_Identity=47.4576271186441, Blast_Score=160, Evalue=4e-40,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 21782; Mature: 21782

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNPQQPLDPGSFDQERAEAAIRELLFAIGEDPDREGLQETPARVARAYRETCAGLYEDP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCH
TEVLNKTFSEDHRELVLVRDITFYSMCEHHLVPFFGKAHIGYIPGESGKVTGLSKLARLI
HHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCHHHHHHHHHH
DGLAKRPQVQERLTSQVADALVDKLDPSAVIVVLEAEHLCMAMRGIRKPGANTVTSAVRG
HHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHCCCCCHHHHHHHHC
GFQKNAASRAEAMALIRP
CHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNPQQPLDPGSFDQERAEAAIRELLFAIGEDPDREGLQETPARVARAYRETCAGLYEDP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCH
TEVLNKTFSEDHRELVLVRDITFYSMCEHHLVPFFGKAHIGYIPGESGKVTGLSKLARLI
HHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCHHHHHHHHHH
DGLAKRPQVQERLTSQVADALVDKLDPSAVIVVLEAEHLCMAMRGIRKPGANTVTSAVRG
HHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHCCCCCHHHHHHHHC
GFQKNAASRAEAMALIRP
CHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA