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The map label for this gene is groEL

Identifier: 68535332

GI number: 68535332

Start: 327201

End: 328844

Strand: Direct

Name: groEL

Synonym: jk0267

Alternate gene names: 68535332

Gene position: 327201-328844 (Clockwise)

Preceding gene: 68535329

Following gene: 68535333

Centisome position: 13.29

GC content: 63.69

Gene sequence:

>1644_bases
ATGGCTAAGATGATTGCATTCGACGAAGAAGCACGCCGCGGACTGGAAAAGGGTCTGAACACCCTGGCAGACGCGGTTAA
GGTAACCCTCGGCCCGAAGGGACGCAACGTTGTCCTGGAGCGCAAGTGGGGCGCCCCGACGATCACCAACGACGGTGTGA
CGATCGCTCGCGAAATCGAGCTGGAAGACCCCTACGAGAAGATCGGCGCCGAGCTGGTTAAGGAAGTCGCCAAGAAGACT
GACGACGTTGCTGGCGATGGCACCACCACCGCTACTGTCCTGGCCCAGTCCCTGGTTCGCGAGGGTCTGCGCAACGTTGC
CGCTGGCTCCAATCCGATGGGCATCAAGCGTGGCATCCAGGCTGGCGTGGAGAAGATCACGGCAGAGCTGCTGAGCCATG
CTAAGGAGATCGAGACCCAGGAGCAGATCGCTGCTACCGCCGGTATCTCCGCTGCTGACGAGAAGATCGGCGAGCTGATC
GCCGAGGCTATGTACAAGGTCGGCGATGGCAAGCTGAACAAGGACGGCGTGATCACTGTCGAGGAGTCCAACGCCTTCGG
TGTGAACCTCGAGGTCACTGAGGGTATGCGCTTCGACAAGGGCTACATCTCCGGCTACTTCGCCACCGACGTGGAGCGTG
GCGAGGCTGTGCTGGAGGATCCGTACATCCTGCTGGTTAGCTCCAAGATCTCCAACGTGAAGGACCTGCTGCCGCTGCTG
GAGAAGGTTATGCAGTCCGGCAAGCCGCTGCTGATCGTTGCCGAGGACATCGAGGGCGAGGCTCTGTCCACCCTGGTTGT
TAACAAGATCCGCGGCACCTTCAAGTCCGTCGCTGTGAAGGCACCGGGCTTCGGCGACCGCCGCAAGGCGCAGCTGCAGG
ACATCGCGATCCTGACCGGTGGCCAGGTCATCGCCGAAGAGGTTGGCCTCTCCCTGGAGACTGCAGACCTGCCGATGCTG
GGTACTGCCCGCAAGGTCGTGGTGACCAAGGACGATACGACCATCGTCGACGGCGCAGGCTCCGCAGAGCAGCTGGCTGG
CCGCATCAAGCAGATCCGCCAGGAAATCGAGAACGCGGACTCCGACTACGACGCTGAGAAGCTGCAGGAGCGCCTGGCTA
AGCTGTCCGGTGGCGTTGCGGTGCTGCAGGTCGGCGCTGCCACCGAGGTTGAGCTGAAGGAGCGCAAGCACCGCATCGAG
GATGCCGTGCGCAACGCTAAGGCAGCCGCGGAAGAGGGAATCGTCGCCGGCGGTGGCGCAGCCCTGCTGCAGACCGCCAG
CGTGCTGGACGACAACCTGGGCCTGGAAGGCGACGAGGCTACCGGCGTGCAGATCGTACGCGCAGCCCTGGCAGCTCCGC
TGAAGCAGATCGCTCACAACGCTGGTCTGGAGCCGGGCGTGGTTGTGGACAAGGTTGCCAACCTGCCGGTAGGCGAGGGG
CTGAACGCTGCAACCGGCGAGTACGTGGACCTGCTGGGTGCTGGCATCTCCGACCCGGTGAAGGTCACCCGTTCCGCACT
GCAGAACGCTGCTTCCATCGCAGCGCTGTTCCTGACGACCGAGGCTGTTGTCGCGGACAAGCCGGAGCCGGAGGCGCCGG
CAATGCCGGGCGCCGACGAGATGGGCGGCATGGGCGGCTTCTAA

Upstream 100 bases:

>100_bases
GTGAGGGTGAATGTCGCGACATTCATTCACCCGTGCCGTCGCGGGCGCTTGATCTGAGCCGTATGTGTCGCCAACTCTAA
ACGGAGGATCGAAAAGCCTC

Downstream 100 bases:

>100_bases
GCCCCGTGTGAAACAACTAAAAAAAGCCTAGTTTTCCTAGAGTTAACCTAGGTGGACTTGCTGTAAAAACAACCCCTGAG
CTGGAACTTTACCAAATCGT

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein 1; Protein Cpn60 1

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MAKMIAFDEEARRGLEKGLNTLADAVKVTLGPKGRNVVLERKWGAPTITNDGVTIAREIELEDPYEKIGAELVKEVAKKT
DDVAGDGTTTATVLAQSLVREGLRNVAAGSNPMGIKRGIQAGVEKITAELLSHAKEIETQEQIAATAGISAADEKIGELI
AEAMYKVGDGKLNKDGVITVEESNAFGVNLEVTEGMRFDKGYISGYFATDVERGEAVLEDPYILLVSSKISNVKDLLPLL
EKVMQSGKPLLIVAEDIEGEALSTLVVNKIRGTFKSVAVKAPGFGDRRKAQLQDIAILTGGQVIAEEVGLSLETADLPML
GTARKVVVTKDDTTIVDGAGSAEQLAGRIKQIRQEIENADSDYDAEKLQERLAKLSGGVAVLQVGAATEVELKERKHRIE
DAVRNAKAAAEEGIVAGGGAALLQTASVLDDNLGLEGDEATGVQIVRAALAAPLKQIAHNAGLEPGVVVDKVANLPVGEG
LNAATGEYVDLLGAGISDPVKVTRSALQNAASIAALFLTTEAVVADKPEPEAPAMPGADEMGGMGGF

Sequences:

>Translated_547_residues
MAKMIAFDEEARRGLEKGLNTLADAVKVTLGPKGRNVVLERKWGAPTITNDGVTIAREIELEDPYEKIGAELVKEVAKKT
DDVAGDGTTTATVLAQSLVREGLRNVAAGSNPMGIKRGIQAGVEKITAELLSHAKEIETQEQIAATAGISAADEKIGELI
AEAMYKVGDGKLNKDGVITVEESNAFGVNLEVTEGMRFDKGYISGYFATDVERGEAVLEDPYILLVSSKISNVKDLLPLL
EKVMQSGKPLLIVAEDIEGEALSTLVVNKIRGTFKSVAVKAPGFGDRRKAQLQDIAILTGGQVIAEEVGLSLETADLPML
GTARKVVVTKDDTTIVDGAGSAEQLAGRIKQIRQEIENADSDYDAEKLQERLAKLSGGVAVLQVGAATEVELKERKHRIE
DAVRNAKAAAEEGIVAGGGAALLQTASVLDDNLGLEGDEATGVQIVRAALAAPLKQIAHNAGLEPGVVVDKVANLPVGEG
LNAATGEYVDLLGAGISDPVKVTRSALQNAASIAALFLTTEAVVADKPEPEAPAMPGADEMGGMGGF
>Mature_546_residues
AKMIAFDEEARRGLEKGLNTLADAVKVTLGPKGRNVVLERKWGAPTITNDGVTIAREIELEDPYEKIGAELVKEVAKKTD
DVAGDGTTTATVLAQSLVREGLRNVAAGSNPMGIKRGIQAGVEKITAELLSHAKEIETQEQIAATAGISAADEKIGELIA
EAMYKVGDGKLNKDGVITVEESNAFGVNLEVTEGMRFDKGYISGYFATDVERGEAVLEDPYILLVSSKISNVKDLLPLLE
KVMQSGKPLLIVAEDIEGEALSTLVVNKIRGTFKSVAVKAPGFGDRRKAQLQDIAILTGGQVIAEEVGLSLETADLPMLG
TARKVVVTKDDTTIVDGAGSAEQLAGRIKQIRQEIENADSDYDAEKLQERLAKLSGGVAVLQVGAATEVELKERKHRIED
AVRNAKAAAEEGIVAGGGAALLQTASVLDDNLGLEGDEATGVQIVRAALAAPLKQIAHNAGLEPGVVVDKVANLPVGEGL
NAATGEYVDLLGAGISDPVKVTRSALQNAASIAALFLTTEAVVADKPEPEAPAMPGADEMGGMGGF

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=533, Percent_Identity=46.3414634146341, Blast_Score=458, Evalue=1e-129,
Organism=Homo sapiens, GI31542947, Length=533, Percent_Identity=46.3414634146341, Blast_Score=458, Evalue=1e-129,
Organism=Homo sapiens, GI38455427, Length=104, Percent_Identity=35.5769230769231, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1790586, Length=532, Percent_Identity=57.5187969924812, Blast_Score=575, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI17555558, Length=534, Percent_Identity=46.2546816479401, Blast_Score=459, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI193210679, Length=211, Percent_Identity=47.8672985781991, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17564182, Length=544, Percent_Identity=25, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323288, Length=529, Percent_Identity=49.3383742911153, Blast_Score=482, Evalue=1e-137,
Organism=Saccharomyces cerevisiae, GI6322524, Length=137, Percent_Identity=32.8467153284672, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320058, Length=184, Percent_Identity=26.6304347826087, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24641193, Length=530, Percent_Identity=47.3584905660377, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24641191, Length=530, Percent_Identity=47.3584905660377, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI45550936, Length=532, Percent_Identity=44.7368421052632, Blast_Score=440, Evalue=1e-123,
Organism=Drosophila melanogaster, GI45550132, Length=532, Percent_Identity=44.7368421052632, Blast_Score=440, Evalue=1e-123,
Organism=Drosophila melanogaster, GI45550935, Length=532, Percent_Identity=44.7368421052632, Blast_Score=440, Evalue=1e-123,
Organism=Drosophila melanogaster, GI17864606, Length=528, Percent_Identity=39.9621212121212, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24584129, Length=544, Percent_Identity=35.8455882352941, Blast_Score=292, Evalue=5e-79,
Organism=Drosophila melanogaster, GI19921262, Length=544, Percent_Identity=35.8455882352941, Blast_Score=292, Evalue=5e-79,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH601_CORJK (Q4JXN8)

Other databases:

- EMBL:   CR931997
- RefSeq:   YP_250037.1
- HSSP:   P0A520
- ProteinModelPortal:   Q4JXN8
- SMR:   Q4JXN8
- STRING:   Q4JXN8
- GeneID:   3433793
- GenomeReviews:   CR931997_GR
- KEGG:   cjk:jk0267
- NMPDR:   fig|306537.3.peg.810
- eggNOG:   COG0459
- HOGENOM:   HBG625289
- OMA:   NSDTSIG
- ProtClustDB:   PRK00013
- BioCyc:   CJEI306537:JK0267-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 57239; Mature: 57108

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKMIAFDEEARRGLEKGLNTLADAVKVTLGPKGRNVVLERKWGAPTITNDGVTIAREIE
CCCCEECCHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEECCCCCCCCCCCEEEEEEEC
LEDPYEKIGAELVKEVAKKTDDVAGDGTTTATVLAQSLVREGLRNVAAGSNPMGIKRGIQ
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
AGVEKITAELLSHAKEIETQEQIAATAGISAADEKIGELIAEAMYKVGDGKLNKDGVITV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
EESNAFGVNLEVTEGMRFDKGYISGYFATDVERGEAVLEDPYILLVSSKISNVKDLLPLL
ECCCCEEEEEEECCCCEECCCHHCCEEECCHHCCCHHHCCCEEEEEECHHHHHHHHHHHH
EKVMQSGKPLLIVAEDIEGEALSTLVVNKIRGTFKSVAVKAPGFGDRRKAQLQDIAILTG
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHEEEEEC
GQVIAEEVGLSLETADLPMLGTARKVVVTKDDTTIVDGAGSAEQLAGRIKQIRQEIENAD
CHHHHHHHCCEEECCCCCCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHCCC
SDYDAEKLQERLAKLSGGVAVLQVGAATEVELKERKHRIEDAVRNAKAAAEEGIVAGGGA
CCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCH
ALLQTASVLDDNLGLEGDEATGVQIVRAALAAPLKQIAHNAGLEPGVVVDKVANLPVGEG
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCC
LNAATGEYVDLLGAGISDPVKVTRSALQNAASIAALFLTTEAVVADKPEPEAPAMPGADE
CCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
MGGMGGF
CCCCCCC
>Mature Secondary Structure 
AKMIAFDEEARRGLEKGLNTLADAVKVTLGPKGRNVVLERKWGAPTITNDGVTIAREIE
CCCEECCHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEECCCCCCCCCCCEEEEEEEC
LEDPYEKIGAELVKEVAKKTDDVAGDGTTTATVLAQSLVREGLRNVAAGSNPMGIKRGIQ
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
AGVEKITAELLSHAKEIETQEQIAATAGISAADEKIGELIAEAMYKVGDGKLNKDGVITV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
EESNAFGVNLEVTEGMRFDKGYISGYFATDVERGEAVLEDPYILLVSSKISNVKDLLPLL
ECCCCEEEEEEECCCCEECCCHHCCEEECCHHCCCHHHCCCEEEEEECHHHHHHHHHHHH
EKVMQSGKPLLIVAEDIEGEALSTLVVNKIRGTFKSVAVKAPGFGDRRKAQLQDIAILTG
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHEEEEEC
GQVIAEEVGLSLETADLPMLGTARKVVVTKDDTTIVDGAGSAEQLAGRIKQIRQEIENAD
CHHHHHHHCCEEECCCCCCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHCCC
SDYDAEKLQERLAKLSGGVAVLQVGAATEVELKERKHRIEDAVRNAKAAAEEGIVAGGGA
CCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCH
ALLQTASVLDDNLGLEGDEATGVQIVRAALAAPLKQIAHNAGLEPGVVVDKVANLPVGEG
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCC
LNAATGEYVDLLGAGISDPVKVTRSALQNAASIAALFLTTEAVVADKPEPEAPAMPGADE
CCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
MGGMGGF
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA