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The map label for this gene is eutD [H]

Identifier: 68535315

GI number: 68535315

Start: 307337

End: 308848

Strand: Direct

Name: eutD [H]

Synonym: jk0250

Alternate gene names: 68535315

Gene position: 307337-308848 (Clockwise)

Preceding gene: 68535314

Following gene: 68535316

Centisome position: 12.48

GC content: 64.88

Gene sequence:

>1512_bases
GTGTCTATCGCGTCGTCTACTGCGTCCCGAGGAACTGGCACGAACGCCGTCATCTCACCGATAGCGAGCGCAGCCGCGCG
CCGCTCTGACATCTTGGAGGCGCTGGGGGAGGCAGAGGCAGTCGACATTTTCGACACCATCCGCCCTTCCGCACCCGACT
TTGCCGCTGCCCTCGACGCGGCCTACGGCGCTCAAGCCAGTGCCAGTGCCAGTGCAGGAGCTGGCGCCGTGCTGCTCGGC
ACCGGCGAGATCAACTTCGACTCGCGCCTCGCCGCCGCCGCGGGTGCCTCCCACTACCTGCTCGCGGGCAGCCCGGCGGA
TGCCACGGCGCTGCAATTGGCCTCCGGGCAGCTCGTCCGCTCCGGCCGTAAGCCTGCCGGTATCTCTTTTATTGACGCCA
ACGCCTCCGCAGGTGCCGCCGCAGCGAACGCCGTCGAAGCGGAGGGGCAGGCGATTCGCCACATCGCGCTGGAAGGCATT
GCGGAAGCAGCTGCCGGCACCAATGGGGAGAGCGCTGCTAAGGAGCCGGTGATTGGTCCGGAGCTTTTCGAGCGCAACCT
GCTTATAAAGGCCCGCGAGAAGGATGCACACATCGTGCTGCCGGAAGGTGACGACGACCGCATCCTCACCGCTGCTGACC
AGCTGCTGCGCGCCAAGGTATGTCGGCTGACCATTCTGGGTAACCCGGACGAGATTGCCGGCCGTGCCAAGGAGCTGGGG
TTGGACCTGTCCGGCGCCACGCTGACCGACCCGAGCGAAGGCAACCAGTTGGAGGAGTTTGCCACCGAGTTCGCCGAGCT
GCGCAAGCACAAGGGGATCACGCTGGAGCAGGCGCGGGAGACGATGCAAGACATCAGCTACTACGCCACGATGATGGTCC
ACAAGGGCATCGCCGACGGCATGGTTTCTGGCGCGGCCCACACGACCGCACACACCATTAAGCCCTCGTTCCAGATCATT
AAGACCGCACCGGGAGCGTCGGTGGTCAGCTCCATCTTCCTGATGGTCATGGATGGTGTGCTGTGGGCATTTGGCGACTG
TGCCGTGAACCCGAACCCTACTTCCGACCAGCTGGCAGAGATCGCCGCCGTGTCCGCCAAAACCGCCGCGCAGTTCGGCA
TTGAGCCAAAGGTAGCGATGCTGAGCTACTCCACCGGAACCTCCGGTGCGGGCGAGGACGTGGAGGCAGTCGCCGCCGCC
GTGAAGAAGGCTCAGGAGGACAACCCGGACCTGCCGCTGGATGGTCCGCTGCAGTTCGATGCCGCAGTGGTGGAATCCGT
CGGCCAGAAGAAGCTGCCGGGCTCTGACGTGGCCGGGCAGGCAACCGTGTTCGTGTTCCCGGACCTGAACTGCGGAAACA
TTACCTACAAGGCGGTTCAGCGCACCGCCGACGCATTGGCTGTGGGGCCGATCCTGCAGGGGCTGAACAAGCCGGTGAAT
GACCTCTCGCGTGGCGCGACCGTGCCAGACATCGTGAACACCGTGGCGATCACTGCGATCCAGGCCAAATAG

Upstream 100 bases:

>100_bases
GTAGGACGAGGGCGAATCTTCCGTGTAGACCTCGCCACCTAGGGGCTGCCAAGGCACAATGGAAGTGAACTGAAACTCCA
TTGAGGAAGGTATGTGATCT

Downstream 100 bases:

>100_bases
CCGGAAAGCCGGCGATCGAGGCGCGAACCGTGCGGTCGTCGGCATTTGCCAGCGGGAACTACCCCGCCCGCTGGCTGAGC
GGCAAAACGGCGCCGGCAGA

Product: phosphotransacetylase

Products: NA

Alternate protein names: Phosphotransacetylase [H]

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MSIASSTASRGTGTNAVISPIASAAARRSDILEALGEAEAVDIFDTIRPSAPDFAAALDAAYGAQASASASAGAGAVLLG
TGEINFDSRLAAAAGASHYLLAGSPADATALQLASGQLVRSGRKPAGISFIDANASAGAAAANAVEAEGQAIRHIALEGI
AEAAAGTNGESAAKEPVIGPELFERNLLIKAREKDAHIVLPEGDDDRILTAADQLLRAKVCRLTILGNPDEIAGRAKELG
LDLSGATLTDPSEGNQLEEFATEFAELRKHKGITLEQARETMQDISYYATMMVHKGIADGMVSGAAHTTAHTIKPSFQII
KTAPGASVVSSIFLMVMDGVLWAFGDCAVNPNPTSDQLAEIAAVSAKTAAQFGIEPKVAMLSYSTGTSGAGEDVEAVAAA
VKKAQEDNPDLPLDGPLQFDAAVVESVGQKKLPGSDVAGQATVFVFPDLNCGNITYKAVQRTADALAVGPILQGLNKPVN
DLSRGATVPDIVNTVAITAIQAK

Sequences:

>Translated_503_residues
MSIASSTASRGTGTNAVISPIASAAARRSDILEALGEAEAVDIFDTIRPSAPDFAAALDAAYGAQASASASAGAGAVLLG
TGEINFDSRLAAAAGASHYLLAGSPADATALQLASGQLVRSGRKPAGISFIDANASAGAAAANAVEAEGQAIRHIALEGI
AEAAAGTNGESAAKEPVIGPELFERNLLIKAREKDAHIVLPEGDDDRILTAADQLLRAKVCRLTILGNPDEIAGRAKELG
LDLSGATLTDPSEGNQLEEFATEFAELRKHKGITLEQARETMQDISYYATMMVHKGIADGMVSGAAHTTAHTIKPSFQII
KTAPGASVVSSIFLMVMDGVLWAFGDCAVNPNPTSDQLAEIAAVSAKTAAQFGIEPKVAMLSYSTGTSGAGEDVEAVAAA
VKKAQEDNPDLPLDGPLQFDAAVVESVGQKKLPGSDVAGQATVFVFPDLNCGNITYKAVQRTADALAVGPILQGLNKPVN
DLSRGATVPDIVNTVAITAIQAK
>Mature_502_residues
SIASSTASRGTGTNAVISPIASAAARRSDILEALGEAEAVDIFDTIRPSAPDFAAALDAAYGAQASASASAGAGAVLLGT
GEINFDSRLAAAAGASHYLLAGSPADATALQLASGQLVRSGRKPAGISFIDANASAGAAAANAVEAEGQAIRHIALEGIA
EAAAGTNGESAAKEPVIGPELFERNLLIKAREKDAHIVLPEGDDDRILTAADQLLRAKVCRLTILGNPDEIAGRAKELGL
DLSGATLTDPSEGNQLEEFATEFAELRKHKGITLEQARETMQDISYYATMMVHKGIADGMVSGAAHTTAHTIKPSFQIIK
TAPGASVVSSIFLMVMDGVLWAFGDCAVNPNPTSDQLAEIAAVSAKTAAQFGIEPKVAMLSYSTGTSGAGEDVEAVAAAV
KKAQEDNPDLPLDGPLQFDAAVVESVGQKKLPGSDVAGQATVFVFPDLNCGNITYKAVQRTADALAVGPILQGLNKPVND
LSRGATVPDIVNTVAITAIQAK

Specific function: Conversion of acetate to acetyl-CoA; second step. [C]

COG id: COG0280

COG function: function code C; Phosphotransacetylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788635, Length=326, Percent_Identity=53.9877300613497, Blast_Score=344, Evalue=6e-96,
Organism=Escherichia coli, GI1788800, Length=321, Percent_Identity=39.2523364485981, Blast_Score=191, Evalue=9e-50,
Organism=Escherichia coli, GI1788806, Length=337, Percent_Identity=29.9703264094956, Blast_Score=166, Evalue=4e-42,

Paralogues:

None

Copy number: 1420 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1250 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012147
- InterPro:   IPR004614
- InterPro:   IPR002505 [H]

Pfam domain/function: PF01515 PTA_PTB [H]

EC number: =2.3.1.8 [H]

Molecular weight: Translated: 51362; Mature: 51231

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIASSTASRGTGTNAVISPIASAAARRSDILEALGEAEAVDIFDTIRPSAPDFAAALDA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHH
AYGAQASASASAGAGAVLLGTGEINFDSRLAAAAGASHYLLAGSPADATALQLASGQLVR
HHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHCCHHHH
SGRKPAGISFIDANASAGAAAANAVEAEGQAIRHIALEGIAEAAAGTNGESAAKEPVIGP
CCCCCCCEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
ELFERNLLIKAREKDAHIVLPEGDDDRILTAADQLLRAKVCRLTILGNPDEIAGRAKELG
HHHHCCCEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHC
LDLSGATLTDPSEGNQLEEFATEFAELRKHKGITLEQARETMQDISYYATMMVHKGIADG
CCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MVSGAAHTTAHTIKPSFQIIKTAPGASVVSSIFLMVMDGVLWAFGDCAVNPNPTSDQLAE
HHCCCCHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IAAVSAKTAAQFGIEPKVAMLSYSTGTSGAGEDVEAVAAAVKKAQEDNPDLPLDGPLQFD
HHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHH
AAVVESVGQKKLPGSDVAGQATVFVFPDLNCGNITYKAVQRTADALAVGPILQGLNKPVN
HHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
DLSRGATVPDIVNTVAITAIQAK
HHHCCCCCHHHHHHHHHEEEECC
>Mature Secondary Structure 
SIASSTASRGTGTNAVISPIASAAARRSDILEALGEAEAVDIFDTIRPSAPDFAAALDA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHH
AYGAQASASASAGAGAVLLGTGEINFDSRLAAAAGASHYLLAGSPADATALQLASGQLVR
HHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHCCHHHH
SGRKPAGISFIDANASAGAAAANAVEAEGQAIRHIALEGIAEAAAGTNGESAAKEPVIGP
CCCCCCCEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
ELFERNLLIKAREKDAHIVLPEGDDDRILTAADQLLRAKVCRLTILGNPDEIAGRAKELG
HHHHCCCEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHC
LDLSGATLTDPSEGNQLEEFATEFAELRKHKGITLEQARETMQDISYYATMMVHKGIADG
CCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MVSGAAHTTAHTIKPSFQIIKTAPGASVVSSIFLMVMDGVLWAFGDCAVNPNPTSDQLAE
HHCCCCHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IAAVSAKTAAQFGIEPKVAMLSYSTGTSGAGEDVEAVAAAVKKAQEDNPDLPLDGPLQFD
HHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHH
AAVVESVGQKKLPGSDVAGQATVFVFPDLNCGNITYKAVQRTADALAVGPILQGLNKPVN
HHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
DLSRGATVPDIVNTVAITAIQAK
HHHCCCCCHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10075432; 12948626 [H]