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The map label for this gene is sseB [H]

Identifier: 68535270

GI number: 68535270

Start: 256300

End: 257187

Strand: Direct

Name: sseB [H]

Synonym: jk0205

Alternate gene names: 68535270

Gene position: 256300-257187 (Clockwise)

Preceding gene: 68535269

Following gene: 68535271

Centisome position: 10.41

GC content: 63.74

Gene sequence:

>888_bases
ATGAGCAACTCTTCTGCCCAACAGCAGCCCGCCAGCCCCTTCGTCTCCGGTGAGGAGATGCTGGAGGCTTTCCAGCGGGG
CGAACGCATGACCGTGATCGACTCGCACTGGGCGCGCACTGAAAACAGCGCCTGGGAGGCATACGTGGGGCAGCACATCC
CCGGTGCCATGTTCTGTAACCCCCTGATGCACCTGGCCTCTATCCCGTCCCGCCGCAAGGGGCGCAACCCTCTGCCTGAG
CCGGAGCGCCTGCAGAAAAGCTTTGACGATTGGGGCATTACGGATGGTCGCCCTGTTTATATTTATGACGCCGGCGCCAA
CCTCTACGCGGCCCGCGCATGGTTCATTTTCCGTTGGGCAGGTGTGCAGAATGTTCGCATCCTGGACGGTGGTACCAAGT
CCTGGGAGTCTGCAGGTGGCGACGTTGCCGGCGGTATCGGGGCTCTGCGCGGCCGCGGCAACGTGGAGGTGCACCCGGGC
AGTATGCCGACCCTCGAACTCGAGGAAGTGGAGCAGTGGCGCGAGGGGCACATCTTGGTGGATGCTCGCGAACCATCCCG
CTATTCCGGCAGGAAGGAACGCTTCGACCACCAGGCGGGCCGCATCCCCGGGGCGATCAACATCCCGGTCAAGGAGCTTT
TCAACAACCGAAGCGAGCGCGACAGTAGCGCCAGCGGCCGCGTGAAAACTCCGGAGGAGATTCAGCGGATTCTCGCCGAC
CATGGCGTGACCAGCGGCGACGACGTGTGCGTTTACTCCGGTTCGGGTCTGCACTCCTCGCTGTTCATCGCTGCGATGGA
GTACGCGGGCCTACCGGGTGCGAGGAACTTCGTCGGAGGTTGGTCCCAATGGTCCGCTTCCGCCTCGCGCCCCATCGAGC
GCGACTAA

Upstream 100 bases:

>100_bases
ACGGGCAAACGGCCGGGTAAAGGGAAACGTACCGGCACGTCTGCCGCAGCGGGATAGCGTGCGCAACATCGGCACGCGCG
AGGTTCTACACTAAGCAACT

Downstream 100 bases:

>100_bases
ACTGCGGGAGTATGGATCCCAACTTTTCCCTGGATCCCCGTAGGCCTGGCCAGCAGCGCGAGCTAGTTGCGCAGAACCAC
TCTGCGAAGTTTCCCGTTGT

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MSNSSAQQQPASPFVSGEEMLEAFQRGERMTVIDSHWARTENSAWEAYVGQHIPGAMFCNPLMHLASIPSRRKGRNPLPE
PERLQKSFDDWGITDGRPVYIYDAGANLYAARAWFIFRWAGVQNVRILDGGTKSWESAGGDVAGGIGALRGRGNVEVHPG
SMPTLELEEVEQWREGHILVDAREPSRYSGRKERFDHQAGRIPGAINIPVKELFNNRSERDSSASGRVKTPEEIQRILAD
HGVTSGDDVCVYSGSGLHSSLFIAAMEYAGLPGARNFVGGWSQWSASASRPIERD

Sequences:

>Translated_295_residues
MSNSSAQQQPASPFVSGEEMLEAFQRGERMTVIDSHWARTENSAWEAYVGQHIPGAMFCNPLMHLASIPSRRKGRNPLPE
PERLQKSFDDWGITDGRPVYIYDAGANLYAARAWFIFRWAGVQNVRILDGGTKSWESAGGDVAGGIGALRGRGNVEVHPG
SMPTLELEEVEQWREGHILVDAREPSRYSGRKERFDHQAGRIPGAINIPVKELFNNRSERDSSASGRVKTPEEIQRILAD
HGVTSGDDVCVYSGSGLHSSLFIAAMEYAGLPGARNFVGGWSQWSASASRPIERD
>Mature_294_residues
SNSSAQQQPASPFVSGEEMLEAFQRGERMTVIDSHWARTENSAWEAYVGQHIPGAMFCNPLMHLASIPSRRKGRNPLPEP
ERLQKSFDDWGITDGRPVYIYDAGANLYAARAWFIFRWAGVQNVRILDGGTKSWESAGGDVAGGIGALRGRGNVEVHPGS
MPTLELEEVEQWREGHILVDAREPSRYSGRKERFDHQAGRIPGAINIPVKELFNNRSERDSSASGRVKTPEEIQRILADH
GVTSGDDVCVYSGSGLHSSLFIAAMEYAGLPGARNFVGGWSQWSASASRPIERD

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=287, Percent_Identity=25.7839721254355, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI61835204, Length=279, Percent_Identity=26.1648745519713, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI194473681, Length=279, Percent_Identity=26.1648745519713, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI17402865, Length=292, Percent_Identity=23.6301369863014, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI87082121, Length=295, Percent_Identity=26.4406779661017, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71997283, Length=262, Percent_Identity=26.3358778625954, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 32525; Mature: 32393

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSSAQQQPASPFVSGEEMLEAFQRGERMTVIDSHWARTENSAWEAYVGQHIPGAMFCN
CCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCHHCCCCCHHHHHHHCCCCHHHHHH
PLMHLASIPSRRKGRNPLPEPERLQKSFDDWGITDGRPVYIYDAGANLYAARAWFIFRWA
HHHHHHCCCCHHCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCEEEHHEEEEEEEC
GVQNVRILDGGTKSWESAGGDVAGGIGALRGRGNVEVHPGSMPTLELEEVEQWREGHILV
CCCEEEEEECCCCCHHHCCCCCCCCHHHHCCCCCEEECCCCCCCEEHHHHHHHHCCCEEE
DAREPSRYSGRKERFDHQAGRIPGAINIPVKELFNNRSERDSSASGRVKTPEEIQRILAD
ECCCCCHHCCHHHHHHHHHCCCCCEEECCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
HGVTSGDDVCVYSGSGLHSSLFIAAMEYAGLPGARNFVGGWSQWSASASRPIERD
CCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCHHHCCCCHHHCCCCCCCCCCC
>Mature Secondary Structure 
SNSSAQQQPASPFVSGEEMLEAFQRGERMTVIDSHWARTENSAWEAYVGQHIPGAMFCN
CCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCHHCCCCCHHHHHHHCCCCHHHHHH
PLMHLASIPSRRKGRNPLPEPERLQKSFDDWGITDGRPVYIYDAGANLYAARAWFIFRWA
HHHHHHCCCCHHCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCEEEHHEEEEEEEC
GVQNVRILDGGTKSWESAGGDVAGGIGALRGRGNVEVHPGSMPTLELEEVEQWREGHILV
CCCEEEEEECCCCCHHHCCCCCCCCHHHHCCCCCEEECCCCCCCEEHHHHHHHHCCCEEE
DAREPSRYSGRKERFDHQAGRIPGAINIPVKELFNNRSERDSSASGRVKTPEEIQRILAD
ECCCCCHHCCHHHHHHHHHCCCCCEEECCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
HGVTSGDDVCVYSGSGLHSSLFIAAMEYAGLPGARNFVGGWSQWSASASRPIERD
CCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCHHHCCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]