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The map label for this gene is grpE [H]

Identifier: 68535245

GI number: 68535245

Start: 227251

End: 227868

Strand: Direct

Name: grpE [H]

Synonym: jk0180

Alternate gene names: 68535245

Gene position: 227251-227868 (Clockwise)

Preceding gene: 68535244

Following gene: 68535246

Centisome position: 9.23

GC content: 65.37

Gene sequence:

>618_bases
ATGGCTGAAGATCTGAATCAGGATCCGGGCGCCCCGGAGAACACTGACCCGGAGGCGACCTCGGCCGAAAGTGCTGAAAC
TGCCGCCGCGGAAGCTGCGGAGGCGCAAGGGGTCGACGAGGGTCTGGTTGACGGTGCTGGGGTTGACGGTGCTGCTGAGG
CCGCTGCGGCTGATGCTGAGGGCGTCAATATCGACGACAGCAACAGCATCGAGGCCGAGCTAGCCGAACGCACCGAGGAT
CTGCAGCGCGTGACGGCGGAGTACACCAACTATCGCCGCCGCGTCGAGCGCGACCGCGCGAGCGTGATCACCGGCGCGAA
GGCCGAGGTTGCTGCCGAGCTGCTGCCGATCCTGGACGACCTGGAGATGGCTGAGCAGCACGGCGACCTGACCGGGCCGC
TGAAGTCCATGTCGGACAAGCTGCAGTCTGTGATGGCTTCCATGAGGGTGGAGAAGTTCGGCGAGGAAGGCGACGAATTC
GACCCGAACTGCCACGAGGCTGTGCAGGACACGAGCAGCGGCGACGACAAGGTGCTTGCCACCGTCCTTCGCCGCGGCTA
CCGCCTGGGCGACCGGGTGCTGCGAAATGCGATGGTCATCATTGGCGATCCGCAGTAG

Upstream 100 bases:

>100_bases
CTGGTGCGGACGCTGCAGGCTCCGCCGCAAACGATGACCCGAACGTTGTGGACGCCGAGGTTGTCGACGAGGACACTTCC
GCAGAGGATGATAAGAAGTA

Downstream 100 bases:

>100_bases
TTATTAAGGGCTAGCGCCAGAGATGGCGTGAGGCTTAGAGAGCTCCATGGCTTATGCTGTGGAGCTCTTTTTGTGTGGGG
TGTATTTGCGAAGGGGTAGG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MAEDLNQDPGAPENTDPEATSAESAETAAAEAAEAQGVDEGLVDGAGVDGAAEAAAADAEGVNIDDSNSIEAELAERTED
LQRVTAEYTNYRRRVERDRASVITGAKAEVAAELLPILDDLEMAEQHGDLTGPLKSMSDKLQSVMASMRVEKFGEEGDEF
DPNCHEAVQDTSSGDDKVLATVLRRGYRLGDRVLRNAMVIIGDPQ

Sequences:

>Translated_205_residues
MAEDLNQDPGAPENTDPEATSAESAETAAAEAAEAQGVDEGLVDGAGVDGAAEAAAADAEGVNIDDSNSIEAELAERTED
LQRVTAEYTNYRRRVERDRASVITGAKAEVAAELLPILDDLEMAEQHGDLTGPLKSMSDKLQSVMASMRVEKFGEEGDEF
DPNCHEAVQDTSSGDDKVLATVLRRGYRLGDRVLRNAMVIIGDPQ
>Mature_204_residues
AEDLNQDPGAPENTDPEATSAESAETAAAEAAEAQGVDEGLVDGAGVDGAAEAAAADAEGVNIDDSNSIEAELAERTEDL
QRVTAEYTNYRRRVERDRASVITGAKAEVAAELLPILDDLEMAEQHGDLTGPLKSMSDKLQSVMASMRVEKFGEEGDEFD
PNCHEAVQDTSSGDDKVLATVLRRGYRLGDRVLRNAMVIIGDPQ

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Escherichia coli, GI1788967, Length=163, Percent_Identity=34.9693251533742, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 21793; Mature: 21662

Theoretical pI: Translated: 3.89; Mature: 3.89

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEDLNQDPGAPENTDPEATSAESAETAAAEAAEAQGVDEGLVDGAGVDGAAEAAAADAE
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHCCCC
GVNIDDSNSIEAELAERTEDLQRVTAEYTNYRRRVERDRASVITGAKAEVAAELLPILDD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LEMAEQHGDLTGPLKSMSDKLQSVMASMRVEKFGEEGDEFDPNCHEAVQDTSSGDDKVLA
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCHHHHH
TVLRRGYRLGDRVLRNAMVIIGDPQ
HHHHHHHHHHHHHHHCCEEEEECCC
>Mature Secondary Structure 
AEDLNQDPGAPENTDPEATSAESAETAAAEAAEAQGVDEGLVDGAGVDGAAEAAAADAE
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHCCCC
GVNIDDSNSIEAELAERTEDLQRVTAEYTNYRRRVERDRASVITGAKAEVAAELLPILDD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LEMAEQHGDLTGPLKSMSDKLQSVMASMRVEKFGEEGDEFDPNCHEAVQDTSSGDDKVLA
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCHHHHH
TVLRRGYRLGDRVLRNAMVIIGDPQ
HHHHHHHHHHHHHHHCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910 [H]