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The map label for this gene is ldh

Identifier: 68535178

GI number: 68535178

Start: 133128

End: 134108

Strand: Direct

Name: ldh

Synonym: jk0113

Alternate gene names: 68535178

Gene position: 133128-134108 (Clockwise)

Preceding gene: 68535175

Following gene: 68535179

Centisome position: 5.41

GC content: 62.69

Gene sequence:

>981_bases
ATGACTGCTCCACAACCGCACACCATTACACCCCCGACCCCCGGCTCCAAGATCGTGCTCATTGGAGCGGGTGACGTCGG
CATCGCCTACGCATACACCCTGGTCAATCAAGGTTTGACCGACCACCTGGCAATCATCGACCTGGATGAGCGCAAGACCT
GGGGCCACGTACAGGACCTCAACCACGCAGTGCCGTGGTCCCACCACAACACCCGCGTGACAGTCGGCACCTACGAGGAC
TGCCGCGATGCCGCGATGGTCTGCATCTGCGCGGGCGCGGCGCAGAAGCCGGGGGAGACTCGACTGGACCTCGTTGCCAA
GAACACCGCAATCTTCAAGACGATCGTCGGCGACGTGATGAGCCACGGCTTCAACGGCATCTTCCTGGTCGCCAGCAACC
CGGTGGACATTTTGAGCTACGCGACGTGGAAGTTCTCCGGCATGGACTCCAGCCGCGTGATCGGCTCCGGCACGATTCTG
GATACCGCGCGCTTCCGCTATGCGCTGGGCCGCTACTTCGACCTCGCGCCGACCTCCGTGCACGCCTACGTGATCGGCGA
GCACGGGGATACCGAGCTGCCGGTGCTATCCGCCGGTTCGGTGGCGGGCACTTCCATCCACCACCGCCTGGAGATGATCG
GCGAATCCGCCGACGAGGACGTCGACGAAATCTTCGTGAAGACCCGCGACGCGGCCTACGAAATCATCCAGGCCAAGGGC
TCCACCAGCTTCGGCATCGGCATGGGGCTGGCGCGCATCACCCAGGCAGTGTTCAGCAACCAGGATGTCGTGCTGCCGAT
CTCCACCCTTTTGCAAGGCGAGTACGGATTCGAGGACATCTACATCGGCACTCCCGCGGTGATTAACCGCCAAGGCGTGC
GCCATGCCGTGGAGCTACAGCTGGATTCCGAAGAAAAGGAGAGGTTCGACCACTCCGCGAATGTGCTGCGCAAGGTAATG
GGCGAGGCCGGGCTGATTTAG

Upstream 100 bases:

>100_bases
AATTCTTGAGGTGAGCGTGTCTGAGCGTAGCGCTATCCTGGCCGTGGGCGTCAAAGAGAACGCAGGCACCTACAGGGCAA
CCAAAAGGAAGAAGGCCACC

Downstream 100 bases:

>100_bases
GCCGGGCGGCTTTAAGCAGCGCTGCATTTAAGTAAACAATAAGTGAAACTTGGAGGCTGCGGGGCGTGCGCGGATGCCGG
TTGCACGCTGGCGCATTAAA

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MTAPQPHTITPPTPGSKIVLIGAGDVGIAYAYTLVNQGLTDHLAIIDLDERKTWGHVQDLNHAVPWSHHNTRVTVGTYED
CRDAAMVCICAGAAQKPGETRLDLVAKNTAIFKTIVGDVMSHGFNGIFLVASNPVDILSYATWKFSGMDSSRVIGSGTIL
DTARFRYALGRYFDLAPTSVHAYVIGEHGDTELPVLSAGSVAGTSIHHRLEMIGESADEDVDEIFVKTRDAAYEIIQAKG
STSFGIGMGLARITQAVFSNQDVVLPISTLLQGEYGFEDIYIGTPAVINRQGVRHAVELQLDSEEKERFDHSANVLRKVM
GEAGLI

Sequences:

>Translated_326_residues
MTAPQPHTITPPTPGSKIVLIGAGDVGIAYAYTLVNQGLTDHLAIIDLDERKTWGHVQDLNHAVPWSHHNTRVTVGTYED
CRDAAMVCICAGAAQKPGETRLDLVAKNTAIFKTIVGDVMSHGFNGIFLVASNPVDILSYATWKFSGMDSSRVIGSGTIL
DTARFRYALGRYFDLAPTSVHAYVIGEHGDTELPVLSAGSVAGTSIHHRLEMIGESADEDVDEIFVKTRDAAYEIIQAKG
STSFGIGMGLARITQAVFSNQDVVLPISTLLQGEYGFEDIYIGTPAVINRQGVRHAVELQLDSEEKERFDHSANVLRKVM
GEAGLI
>Mature_325_residues
TAPQPHTITPPTPGSKIVLIGAGDVGIAYAYTLVNQGLTDHLAIIDLDERKTWGHVQDLNHAVPWSHHNTRVTVGTYEDC
RDAAMVCICAGAAQKPGETRLDLVAKNTAIFKTIVGDVMSHGFNGIFLVASNPVDILSYATWKFSGMDSSRVIGSGTILD
TARFRYALGRYFDLAPTSVHAYVIGEHGDTELPVLSAGSVAGTSIHHRLEMIGESADEDVDEIFVKTRDAAYEIIQAKGS
TSFGIGMGLARITQAVFSNQDVVLPISTLLQGEYGFEDIYIGTPAVINRQGVRHAVELQLDSEEKERFDHSANVLRKVMG
EAGLI

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family

Homologues:

Organism=Homo sapiens, GI5031857, Length=312, Percent_Identity=38.7820512820513, Blast_Score=233, Evalue=1e-61,
Organism=Homo sapiens, GI260099723, Length=312, Percent_Identity=38.7820512820513, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI15082234, Length=316, Percent_Identity=36.3924050632911, Blast_Score=231, Evalue=8e-61,
Organism=Homo sapiens, GI47059044, Length=312, Percent_Identity=37.5, Blast_Score=228, Evalue=8e-60,
Organism=Homo sapiens, GI221136809, Length=312, Percent_Identity=37.5, Blast_Score=228, Evalue=8e-60,
Organism=Homo sapiens, GI291575128, Length=305, Percent_Identity=35.4098360655738, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI4557032, Length=305, Percent_Identity=35.4098360655738, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI9257228, Length=306, Percent_Identity=38.8888888888889, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI4504973, Length=306, Percent_Identity=38.8888888888889, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI260099727, Length=208, Percent_Identity=41.8269230769231, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI260099725, Length=229, Percent_Identity=39.3013100436681, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI207028494, Length=212, Percent_Identity=37.7358490566038, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI103472011, Length=313, Percent_Identity=24.6006389776358, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI103472015, Length=179, Percent_Identity=29.608938547486, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17535107, Length=295, Percent_Identity=40, Blast_Score=227, Evalue=7e-60,
Organism=Caenorhabditis elegans, GI17554310, Length=304, Percent_Identity=25.6578947368421, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322765, Length=301, Percent_Identity=28.2392026578073, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17136226, Length=317, Percent_Identity=36.9085173501577, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI45550422, Length=306, Percent_Identity=30.0653594771242, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24647881, Length=315, Percent_Identity=26.031746031746, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): LDH_CORJK (Q4JY42)

Other databases:

- EMBL:   CR931997
- RefSeq:   YP_249883.1
- ProteinModelPortal:   Q4JY42
- SMR:   Q4JY42
- STRING:   Q4JY42
- GeneID:   3432477
- GenomeReviews:   CR931997_GR
- KEGG:   cjk:jk0113
- NMPDR:   fig|306537.3.peg.2127
- eggNOG:   COG0039
- HOGENOM:   HBG566126
- OMA:   WSSANIG
- ProtClustDB:   PRK00066
- BioCyc:   CJEI306537:JK0113-MONOMER
- GO:   GO:0005737
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00488
- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01771

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.27

Molecular weight: Translated: 35237; Mature: 35106

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00064 L_LDH

Important sites: ACT_SITE 188-188 BINDING 101-101 BINDING 133-133 BINDING 164-164 BINDING 242-242

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPQPHTITPPTPGSKIVLIGAGDVGIAYAYTLVNQGLTDHLAIIDLDERKTWGHVQDL
CCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHC
NHAVPWSHHNTRVTVGTYEDCRDAAMVCICAGAAQKPGETRLDLVAKNTAIFKTIVGDVM
CCCCCCCCCCCEEEECCHHHHHHHHEEEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHH
SHGFNGIFLVASNPVDILSYATWKFSGMDSSRVIGSGTILDTARFRYALGRYFDLAPTSV
HCCCCEEEEEECCCHHHHEEEEEEECCCCCCCEEECCCEEHHHHHHHHHHHHEECCCCEE
HAYVIGEHGDTELPVLSAGSVAGTSIHHRLEMIGESADEDVDEIFVKTRDAAYEIIQAKG
EEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHCCC
STSFGIGMGLARITQAVFSNQDVVLPISTLLQGEYGFEDIYIGTPAVINRQGVRHAVELQ
CCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCEEEECCCHHCCCCCCCEEEEEE
LDSEEKERFDHSANVLRKVMGEAGLI
ECCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TAPQPHTITPPTPGSKIVLIGAGDVGIAYAYTLVNQGLTDHLAIIDLDERKTWGHVQDL
CCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHC
NHAVPWSHHNTRVTVGTYEDCRDAAMVCICAGAAQKPGETRLDLVAKNTAIFKTIVGDVM
CCCCCCCCCCCEEEECCHHHHHHHHEEEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHH
SHGFNGIFLVASNPVDILSYATWKFSGMDSSRVIGSGTILDTARFRYALGRYFDLAPTSV
HCCCCEEEEEECCCHHHHEEEEEEECCCCCCCEEECCCEEHHHHHHHHHHHHEECCCCEE
HAYVIGEHGDTELPVLSAGSVAGTSIHHRLEMIGESADEDVDEIFVKTRDAAYEIIQAKG
EEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHCCC
STSFGIGMGLARITQAVFSNQDVVLPISTLLQGEYGFEDIYIGTPAVINRQGVRHAVELQ
CCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCEEEECCCHHCCCCCCCEEEEEE
LDSEEKERFDHSANVLRKVMGEAGLI
ECCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA