Click here to switch to the map view.

The map label for this gene is metB [H]

Identifier: 68535117

GI number: 68535117

Start: 61219

End: 62382

Strand: Direct

Name: metB [H]

Synonym: jk0055

Alternate gene names: 68535117

Gene position: 61219-62382 (Clockwise)

Preceding gene: 68535115

Following gene: 68535118

Centisome position: 2.49

GC content: 63.14

Gene sequence:

>1164_bases
ATGGCCCACAACACAGACGCCATCCACGCAGGCTACGAGCCCGACGAGTTCATGGGCTCCATCAACATTCCGATCTACGC
TTCCACCACGTTCGCCCAGAACGCGCCGAACGATCTGCGCGGAGGCTACGAATACGGGCGCGTGGCGAACCCCACTGTCG
ACTCGCTGGCCCGTACAATCGCCGCCCTCGAGGGGGGCGAGCACGGTCGCGTGTACTCCTCTGGCATGGCCGCCACCGAC
CACCTGCTGCGCGTGCTTTTGCGCCCCGGCGACCATCTGATCATGGGGCACGACGCTTACGGCGGCACGTATCGCCTGAT
CGCCACGGTATTCAAGGACTGGAACGTGGACTTCACCGTGGTTGATACGACAGACCCCGAGGCCGTCCGTGCGGCGCTGA
CCCCGAAGACGAAGCTGGTGTGGCTGGAGACCCCTACGAATCCACTGCTCGCTGTAACTGACATCGCCGCCATCGCCGAG
GTGCTCGCCGACGAGTTTGGTAGTGACGCCCCCACGCGCCCGAAGCTGATCGTGGATAACACCTTCTGCAGCCCCTACCT
GCAGCGCCCCCTGGATCACGGCGCGGACGTGGTGCTGCACTCCACCACGAAATACATCGGTGGGCACTCCGATGTGGTCG
GCGGTGCGATCGTCAGCAACGACGCACAGCTGGACGAGGATCTGGACTTCCTACTCGGCGGTGCCGGTCCGATTGCCTCG
CCTTTCGATGCCTACCTGAATGCCCGTGGTCTGAAGACCCTGGGTGTGCGCATGGACCGCCACTGCTCGAATGCGCAGGC
TATTGCTGAATATCTGGAAGAGCGGGAGGAAGTCGCCACCGTCCTGTACCCCGGCTTGCCGCAGCATCCAGGACACGAGG
TGGCCAAGCGCCAGTCCACCGGCAAGGGGTTTGGTGGCATGATCTCCGTACGCTTCCACTCTGAGGAGGCGGCACTGAAG
TTCTGCCAGGCCACCAAGCTGATCTGCCTGGCGGAGTCGCTGGGTGGCGTGGAATCTCTGCTGGAACACCCCGCGACGAT
GACTCACCAGTCGGTTGCGGGATCCCAGCTGGAGGTTCCGCGCGACCTGGTGCGCATTTCCATTGGTATTGAGGATCTGG
AGGATCTGCTCGCAGATGTCGCGCAGGCTCTGGAACAGCTTTAA

Upstream 100 bases:

>100_bases
TTGGTCTATAATCGTTGAAACGATGCGCGCCGGGCCGTGAATTCCGGCGCAGATAAGCACCAAAACGCGCAATCAAGCAC
TAGTTCGAAGGGGAGTTTCC

Downstream 100 bases:

>100_bases
AATACGGGGCTTATGAAGTCCTTTTCTCCTTCCAAGTCCCTCACCGCCCTAGCCACCGCAAGCGCCCTAGCCGTCAGCGC
CATCGCAGCCACCCCGGCGC

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MAHNTDAIHAGYEPDEFMGSINIPIYASTTFAQNAPNDLRGGYEYGRVANPTVDSLARTIAALEGGEHGRVYSSGMAATD
HLLRVLLRPGDHLIMGHDAYGGTYRLIATVFKDWNVDFTVVDTTDPEAVRAALTPKTKLVWLETPTNPLLAVTDIAAIAE
VLADEFGSDAPTRPKLIVDNTFCSPYLQRPLDHGADVVLHSTTKYIGGHSDVVGGAIVSNDAQLDEDLDFLLGGAGPIAS
PFDAYLNARGLKTLGVRMDRHCSNAQAIAEYLEEREEVATVLYPGLPQHPGHEVAKRQSTGKGFGGMISVRFHSEEAALK
FCQATKLICLAESLGGVESLLEHPATMTHQSVAGSQLEVPRDLVRISIGIEDLEDLLADVAQALEQL

Sequences:

>Translated_387_residues
MAHNTDAIHAGYEPDEFMGSINIPIYASTTFAQNAPNDLRGGYEYGRVANPTVDSLARTIAALEGGEHGRVYSSGMAATD
HLLRVLLRPGDHLIMGHDAYGGTYRLIATVFKDWNVDFTVVDTTDPEAVRAALTPKTKLVWLETPTNPLLAVTDIAAIAE
VLADEFGSDAPTRPKLIVDNTFCSPYLQRPLDHGADVVLHSTTKYIGGHSDVVGGAIVSNDAQLDEDLDFLLGGAGPIAS
PFDAYLNARGLKTLGVRMDRHCSNAQAIAEYLEEREEVATVLYPGLPQHPGHEVAKRQSTGKGFGGMISVRFHSEEAALK
FCQATKLICLAESLGGVESLLEHPATMTHQSVAGSQLEVPRDLVRISIGIEDLEDLLADVAQALEQL
>Mature_386_residues
AHNTDAIHAGYEPDEFMGSINIPIYASTTFAQNAPNDLRGGYEYGRVANPTVDSLARTIAALEGGEHGRVYSSGMAATDH
LLRVLLRPGDHLIMGHDAYGGTYRLIATVFKDWNVDFTVVDTTDPEAVRAALTPKTKLVWLETPTNPLLAVTDIAAIAEV
LADEFGSDAPTRPKLIVDNTFCSPYLQRPLDHGADVVLHSTTKYIGGHSDVVGGAIVSNDAQLDEDLDFLLGGAGPIASP
FDAYLNARGLKTLGVRMDRHCSNAQAIAEYLEEREEVATVLYPGLPQHPGHEVAKRQSTGKGFGGMISVRFHSEEAALKF
CQATKLICLAESLGGVESLLEHPATMTHQSVAGSQLEVPRDLVRISIGIEDLEDLLADVAQALEQL

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=386, Percent_Identity=45.0777202072539, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI299473759, Length=386, Percent_Identity=41.4507772020725, Blast_Score=251, Evalue=6e-67,
Organism=Homo sapiens, GI25453487, Length=386, Percent_Identity=38.3419689119171, Blast_Score=221, Evalue=7e-58,
Organism=Escherichia coli, GI1790375, Length=380, Percent_Identity=44.4736842105263, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1789383, Length=399, Percent_Identity=30.0751879699248, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17538055, Length=387, Percent_Identity=46.2532299741602, Blast_Score=317, Evalue=9e-87,
Organism=Caenorhabditis elegans, GI17560056, Length=387, Percent_Identity=45.2196382428941, Blast_Score=311, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI71980708, Length=377, Percent_Identity=38.7267904509284, Blast_Score=244, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6319307, Length=391, Percent_Identity=47.5703324808184, Blast_Score=308, Evalue=1e-84,
Organism=Saccharomyces cerevisiae, GI6321254, Length=410, Percent_Identity=35.8536585365854, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6323333, Length=441, Percent_Identity=31.2925170068027, Blast_Score=159, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6321170, Length=336, Percent_Identity=30.6547619047619, Blast_Score=132, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6321904, Length=404, Percent_Identity=28.4653465346535, Blast_Score=121, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19922554, Length=388, Percent_Identity=45.1030927835052, Blast_Score=315, Evalue=3e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 41622; Mature: 41491

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHNTDAIHAGYEPDEFMGSINIPIYASTTFAQNAPNDLRGGYEYGRVANPTVDSLARTI
CCCCCCCEECCCCHHHHCCCCCCEEEEECCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHH
AALEGGEHGRVYSSGMAATDHLLRVLLRPGDHLIMGHDAYGGTYRLIATVFKDWNVDFTV
HHHCCCCCCCEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEE
VDTTDPEAVRAALTPKTKLVWLETPTNPLLAVTDIAAIAEVLADEFGSDAPTRPKLIVDN
EECCCHHHHHHHCCCCEEEEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
TFCSPYLQRPLDHGADVVLHSTTKYIGGHSDVVGGAIVSNDAQLDEDLDFLLGGAGPIAS
CCCCHHHHCHHCCCCCEEEECCHHHCCCCCCCCCCEEECCCCCCCCCHHHHCCCCCCCCC
PFDAYLNARGLKTLGVRMDRHCSNAQAIAEYLEEREEVATVLYPGLPQHPGHEVAKRQST
HHHHHHCCCCCHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCC
GKGFGGMISVRFHSEEAALKFCQATKLICLAESLGGVESLLEHPATMTHQSVAGSQLEVP
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCHHHHHHHHCCCCCCCC
RDLVRISIGIEDLEDLLADVAQALEQL
HHHHEEECCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AHNTDAIHAGYEPDEFMGSINIPIYASTTFAQNAPNDLRGGYEYGRVANPTVDSLARTI
CCCCCCEECCCCHHHHCCCCCCEEEEECCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHH
AALEGGEHGRVYSSGMAATDHLLRVLLRPGDHLIMGHDAYGGTYRLIATVFKDWNVDFTV
HHHCCCCCCCEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEE
VDTTDPEAVRAALTPKTKLVWLETPTNPLLAVTDIAAIAEVLADEFGSDAPTRPKLIVDN
EECCCHHHHHHHCCCCEEEEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
TFCSPYLQRPLDHGADVVLHSTTKYIGGHSDVVGGAIVSNDAQLDEDLDFLLGGAGPIAS
CCCCHHHHCHHCCCCCEEEECCHHHCCCCCCCCCCEEECCCCCCCCCHHHHCCCCCCCCC
PFDAYLNARGLKTLGVRMDRHCSNAQAIAEYLEEREEVATVLYPGLPQHPGHEVAKRQST
HHHHHHCCCCCHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCC
GKGFGGMISVRFHSEEAALKFCQATKLICLAESLGGVESLLEHPATMTHQSVAGSQLEVP
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCHHHHHHHHCCCCCCCC
RDLVRISIGIEDLEDLLADVAQALEQL
HHHHEEECCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]