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The map label for this gene is nolF [H]

Identifier: 66768291

GI number: 66768291

Start: 2376198

End: 2377313

Strand: Direct

Name: nolF [H]

Synonym: XC_1970

Alternate gene names: 66768291

Gene position: 2376198-2377313 (Clockwise)

Preceding gene: 66768288

Following gene: 66768292

Centisome position: 46.15

GC content: 67.11

Gene sequence:

>1116_bases
TTGCACGGCCGCAGCGGACCTTGCGCTGCGGCGCTGTTGATCACCACGAGCCTGTTGCTGGGCGGTTGCAAGGCTGGCGA
CAGTGCTGCCAAGCCGGCAGACAGCCAGAAGGCCGCAGATGCCGTGCCGGTGGAAACCGCCAAGGCAGCCCGTCGCGCGG
TGGCTGCCAGCTACACCGGCACTGCGGCATTGGAGCCACGTGCCGAAGCGCAGGTGGTGGCCAAGACCTCCGGCGTGGCG
CTGGCGGTGATGGTCGAGGAAGGGCAGAAGGTGTCGGCAGGCCAGGCGCTGGTACGGCTGGATCCGGACCGCGCGCATCT
GGCGGTGGCGCAAAGCGAGGCGCAGCTGCGCAAGCTGGAAAACAGCTATCGCCGCGCCACGCAACTGGTCGGCCAGCAAC
TGGTCAGCGCCGCGGATGTGGACCAGCTCAAGTTCGATGTGGAAAACAGCCGCGCGCAGCATCGTCTGGCGTCGCTGGAA
CTGTCGTACACCACCGTGCAGGCACCGATTTCCGGTGTGATTGCTTCGCGCTCGATCAAGACCGGCAACTTCGTGCAGAT
CAATACGCCGATCTTCCGCATCGTCGACGACTCGCAACTGGAAGCCACGCTCAACGTGCCCGAGCGCGAGCTGGCCACGC
TCAAGTCCGGCCAGCCGGTGACGTTGCTGGCCGACGCATTGCCGGGACAGCAATTCGTGGGCAAGGTGGACCGCATCGCG
CCGGTGGTGGATTCGGGCAGCGGCACTTTTCGCGTGGTGTGCGCGTTCGGGGCTGGCGCGGAGGCGTTGCAGCCCGGCAT
GTTCGGGCGCATCCGCATCGACTACGACCAGCGCAAGGATGCCCTGGTGATTCCGCGGCTGGCGCTGCTGGATGACGGTG
AGCCGGCAGTGTTCGTGGTGCGCAATGGCAAGGCCAGCCGGGTGCCGGTGAAGCTGGGCTATGCCGAAGGCCCATGGCTG
GAAGTGCGCCAGGGCCTGAAGGAAGGCGACCAGGTCGTGACGGCCGGCAAGGTGGCGTTGCGCGATGGCACCGCGGTGCA
GATCATCGGCCAGCCCGATCGCAAGCCGGTTGCAGCTGCCGCACCGGCAACGGCCGGCGACGAGAAGCGCTCATGA

Upstream 100 bases:

>100_bases
ACCTTTGGCGCATTGGCGGCATGATGGCTTGGGTGGTTTACTTGCCTACAACACCTGTTCACGCATCCACTAGGACACGA
CGATGTCGCCACTCTCCTTC

Downstream 100 bases:

>100_bases
CCGACCATCCCACCGGCCCGGTCCACGACCCGCACCTATCCGTAGCGCCGGGCGGCGGCCTGGTGGCGTTTGCCACACGC
CGCCGCGTCACCATCGCGAT

Product: cation efflux system protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MHGRSGPCAAALLITTSLLLGGCKAGDSAAKPADSQKAADAVPVETAKAARRAVAASYTGTAALEPRAEAQVVAKTSGVA
LAVMVEEGQKVSAGQALVRLDPDRAHLAVAQSEAQLRKLENSYRRATQLVGQQLVSAADVDQLKFDVENSRAQHRLASLE
LSYTTVQAPISGVIASRSIKTGNFVQINTPIFRIVDDSQLEATLNVPERELATLKSGQPVTLLADALPGQQFVGKVDRIA
PVVDSGSGTFRVVCAFGAGAEALQPGMFGRIRIDYDQRKDALVIPRLALLDDGEPAVFVVRNGKASRVPVKLGYAEGPWL
EVRQGLKEGDQVVTAGKVALRDGTAVQIIGQPDRKPVAAAAPATAGDEKRS

Sequences:

>Translated_371_residues
MHGRSGPCAAALLITTSLLLGGCKAGDSAAKPADSQKAADAVPVETAKAARRAVAASYTGTAALEPRAEAQVVAKTSGVA
LAVMVEEGQKVSAGQALVRLDPDRAHLAVAQSEAQLRKLENSYRRATQLVGQQLVSAADVDQLKFDVENSRAQHRLASLE
LSYTTVQAPISGVIASRSIKTGNFVQINTPIFRIVDDSQLEATLNVPERELATLKSGQPVTLLADALPGQQFVGKVDRIA
PVVDSGSGTFRVVCAFGAGAEALQPGMFGRIRIDYDQRKDALVIPRLALLDDGEPAVFVVRNGKASRVPVKLGYAEGPWL
EVRQGLKEGDQVVTAGKVALRDGTAVQIIGQPDRKPVAAAAPATAGDEKRS
>Mature_371_residues
MHGRSGPCAAALLITTSLLLGGCKAGDSAAKPADSQKAADAVPVETAKAARRAVAASYTGTAALEPRAEAQVVAKTSGVA
LAVMVEEGQKVSAGQALVRLDPDRAHLAVAQSEAQLRKLENSYRRATQLVGQQLVSAADVDQLKFDVENSRAQHRLASLE
LSYTTVQAPISGVIASRSIKTGNFVQINTPIFRIVDDSQLEATLNVPERELATLKSGQPVTLLADALPGQQFVGKVDRIA
PVVDSGSGTFRVVCAFGAGAEALQPGMFGRIRIDYDQRKDALVIPRLALLDDGEPAVFVVRNGKASRVPVKLGYAEGPWL
EVRQGLKEGDQVVTAGKVALRDGTAVQIIGQPDRKPVAAAAPATAGDEKRS

Specific function: Involved in the production of Medicago-specific nodulation signal molecule [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=331, Percent_Identity=28.0966767371601, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789665, Length=378, Percent_Identity=27.7777777777778, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1786668, Length=376, Percent_Identity=28.4574468085106, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 38896; Mature: 38896

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGRSGPCAAALLITTSLLLGGCKAGDSAAKPADSQKAADAVPVETAKAARRAVAASYTG
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
TAALEPRAEAQVVAKTSGVALAVMVEEGQKVSAGQALVRLDPDRAHLAVAQSEAQLRKLE
CEEECCCCCEEEEEECCCEEEEEEECCCCEECCCCEEEEECCCHHEEEEEHHHHHHHHHH
NSYRRATQLVGQQLVSAADVDQLKFDVENSRAQHRLASLELSYTTVQAPISGVIASRSIK
HHHHHHHHHHHHHHHHHCCHHHEEEECCCCHHHHHHHEEEEEEEEECCCHHHHHHHCEEC
TGNFVQINTPIFRIVDDSQLEATLNVPERELATLKSGQPVTLLADALPGQQFVGKVDRIA
CCCEEEECCCEEEEECCCCEEEEECCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHH
PVVDSGSGTFRVVCAFGAGAEALQPGMFGRIRIDYDQRKDALVIPRLALLDDGEPAVFVV
HEEECCCCCEEEEEEECCCHHHHCCCCEEEEEEECCCCCCEEEECEEEEECCCCCEEEEE
RNGKASRVPVKLGYAEGPWLEVRQGLKEGDQVVTAGKVALRDGTAVQIIGQPDRKPVAAA
ECCCCCCCEEEECCCCCCHHHHHHHHCCCCEEEEECEEEEECCCEEEEEECCCCCCEEEC
APATAGDEKRS
CCCCCCCCCCC
>Mature Secondary Structure
MHGRSGPCAAALLITTSLLLGGCKAGDSAAKPADSQKAADAVPVETAKAARRAVAASYTG
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
TAALEPRAEAQVVAKTSGVALAVMVEEGQKVSAGQALVRLDPDRAHLAVAQSEAQLRKLE
CEEECCCCCEEEEEECCCEEEEEEECCCCEECCCCEEEEECCCHHEEEEEHHHHHHHHHH
NSYRRATQLVGQQLVSAADVDQLKFDVENSRAQHRLASLELSYTTVQAPISGVIASRSIK
HHHHHHHHHHHHHHHHHCCHHHEEEECCCCHHHHHHHEEEEEEEEECCCHHHHHHHCEEC
TGNFVQINTPIFRIVDDSQLEATLNVPERELATLKSGQPVTLLADALPGQQFVGKVDRIA
CCCEEEECCCEEEEECCCCEEEEECCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHH
PVVDSGSGTFRVVCAFGAGAEALQPGMFGRIRIDYDQRKDALVIPRLALLDDGEPAVFVV
HEEECCCCCEEEEEEECCCHHHHCCCCEEEEEEECCCCCCEEEECEEEEECCCCCEEEEE
RNGKASRVPVKLGYAEGPWLEVRQGLKEGDQVVTAGKVALRDGTAVQIIGQPDRKPVAAA
ECCCCCCCEEEECCCCCCHHHHHHHHCCCCEEEEECEEEEECCCEEEEEECCCCCCEEEC
APATAGDEKRS
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1909418; 11481432 [H]