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The map label for this gene is purR [H]

Identifier: 66768230

GI number: 66768230

Start: 2304283

End: 2305305

Strand: Direct

Name: purR [H]

Synonym: XC_1909

Alternate gene names: 66768230

Gene position: 2304283-2305305 (Clockwise)

Preceding gene: 66768229

Following gene: 66768231

Centisome position: 44.75

GC content: 69.7

Gene sequence:

>1023_bases
ATGGCCAAAGGTGCGGTCACCATCAAAGATGTCGCTCGAGAAGCACGCGTCTCCGTTGCCACGGTATCGCGGGCGCTCAA
TGGGCACGACAACGTCGCCGGGCCGGTGCGCCAGGTGGTCCTGGACGTGGCCGAACGCCTGCGCTACAGCCCGCATGCGG
CGGCACGCAGCCTCAGCAGCCGGCGCACCCAGACCCTGGGTGTGGTGCTGCCTGATCTGTACGGCGAGTTCTTTTCCGAA
CTGATTCGCGGCATCGATGGGGCGGCGCGTGCGGCCGGGCAGCATTTGCTGGTCTCCAGCTACCACGGCGATCCCGAAGC
CCAGGGCAGGGCGCTGCGTTCCATGCGCGGCCGCGTGGATGGCTTGCTGGTGTTGTCCCCGTACGCCGAGCAGCCGGGTT
TTCTCACCGACAACCTGCCGCAGGCACTGCCCACCGTCCTGATCAACGCGCATCTGCCGGGCGCCGACTACCCGGTGCTG
AGCATCGACGACCACGCCGGCGCAATGGCGATGACCGAGCACCTGCTTGCTGCCGGGCACCGCCGCATCGCCTTCATCGG
CGGGCCGGCGCTGAACTTCGATGCGCGCGAGCGCTTGCGCGGCTTCCGTGACGCGATCGCCGGCCATGCGGGCGGAGCGG
AGGGGCTCGAATTCGCCGGCGACTTCGACGAAGCCTCCGGCCATCGCGCCGGCCTTGCCATGCTGGCCGCCGGGGATCTG
CCCGATGCGGTCTTCGCTGCCAATGACATGACCGCGCTCGGCTGCCTGTACGCCTTCGCCCAGGCGGATGTGTCGGTGCA
CGACGACGTGGCGCTGGCCGGGTTCGACGACATCCCGCTGGCGCGCTTCGTGCACCCCGCGCTGACCACGATGCGGGTCA
GCATTGCGCGCCTTGGCGAGCAGGCGATGACCCGTCTGATGCGGCTGGTGGACCAGCCCGGCCACGACGACGGCATCCGC
CAGTTGCTCACCCCGGAGCTGGTGGTGCGTGCGTCCTGCGGAGCCGGCGAGCCCGGCGCCTGA

Upstream 100 bases:

>100_bases
AGCGTTTTCACAAGCCCATTCACAGGGGTTTACGCCTCCGTATGAAAACGCTTACAGTCCGCGCCAGATTTTGGGAAGTC
GGTCGCGGAGGGCGACCCGA

Downstream 100 bases:

>100_bases
CCCTTTTCGCGGACGTCCTGTCCGCTCCCGCTGCCTCCCCCGCAGCGGTTGTTCCTGCAAGACCTTAGTGCCCACAACAA
CAAGATATCGATCCCTGGAG

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MAKGAVTIKDVAREARVSVATVSRALNGHDNVAGPVRQVVLDVAERLRYSPHAAARSLSSRRTQTLGVVLPDLYGEFFSE
LIRGIDGAARAAGQHLLVSSYHGDPEAQGRALRSMRGRVDGLLVLSPYAEQPGFLTDNLPQALPTVLINAHLPGADYPVL
SIDDHAGAMAMTEHLLAAGHRRIAFIGGPALNFDARERLRGFRDAIAGHAGGAEGLEFAGDFDEASGHRAGLAMLAAGDL
PDAVFAANDMTALGCLYAFAQADVSVHDDVALAGFDDIPLARFVHPALTTMRVSIARLGEQAMTRLMRLVDQPGHDDGIR
QLLTPELVVRASCGAGEPGA

Sequences:

>Translated_340_residues
MAKGAVTIKDVAREARVSVATVSRALNGHDNVAGPVRQVVLDVAERLRYSPHAAARSLSSRRTQTLGVVLPDLYGEFFSE
LIRGIDGAARAAGQHLLVSSYHGDPEAQGRALRSMRGRVDGLLVLSPYAEQPGFLTDNLPQALPTVLINAHLPGADYPVL
SIDDHAGAMAMTEHLLAAGHRRIAFIGGPALNFDARERLRGFRDAIAGHAGGAEGLEFAGDFDEASGHRAGLAMLAAGDL
PDAVFAANDMTALGCLYAFAQADVSVHDDVALAGFDDIPLARFVHPALTTMRVSIARLGEQAMTRLMRLVDQPGHDDGIR
QLLTPELVVRASCGAGEPGA
>Mature_339_residues
AKGAVTIKDVAREARVSVATVSRALNGHDNVAGPVRQVVLDVAERLRYSPHAAARSLSSRRTQTLGVVLPDLYGEFFSEL
IRGIDGAARAAGQHLLVSSYHGDPEAQGRALRSMRGRVDGLLVLSPYAEQPGFLTDNLPQALPTVLINAHLPGADYPVLS
IDDHAGAMAMTEHLLAAGHRRIAFIGGPALNFDARERLRGFRDAIAGHAGGAEGLEFAGDFDEASGHRAGLAMLAAGDLP
DAVFAANDMTALGCLYAFAQADVSVHDDVALAGFDDIPLARFVHPALTTMRVSIARLGEQAMTRLMRLVDQPGHDDGIRQ
LLTPELVVRASCGAGEPGA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=328, Percent_Identity=35.6707317073171, Blast_Score=171, Evalue=9e-44,
Organism=Escherichia coli, GI1789202, Length=332, Percent_Identity=34.3373493975904, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1787948, Length=336, Percent_Identity=33.6309523809524, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1790369, Length=341, Percent_Identity=31.9648093841642, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1788474, Length=301, Percent_Identity=33.2225913621262, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1789068, Length=316, Percent_Identity=31.0126582278481, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1786540, Length=336, Percent_Identity=29.7619047619048, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787580, Length=319, Percent_Identity=27.2727272727273, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI48994940, Length=315, Percent_Identity=28.2539682539683, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789456, Length=314, Percent_Identity=28.6624203821656, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787906, Length=307, Percent_Identity=26.7100977198697, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790715, Length=311, Percent_Identity=23.1511254019293, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786268, Length=313, Percent_Identity=22.0447284345048, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35884; Mature: 35753

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGAVTIKDVAREARVSVATVSRALNGHDNVAGPVRQVVLDVAERLRYSPHAAARSLSS
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RRTQTLGVVLPDLYGEFFSELIRGIDGAARAAGQHLLVSSYHGDPEAQGRALRSMRGRVD
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCC
GLLVLSPYAEQPGFLTDNLPQALPTVLINAHLPGADYPVLSIDDHAGAMAMTEHLLAAGH
EEEEECCCCCCCCCCCCCHHHHHHHHEEECCCCCCCCCEEEECCCCCHHHHHHHHHHCCC
RRIAFIGGPALNFDARERLRGFRDAIAGHAGGAEGLEFAGDFDEASGHRAGLAMLAAGDL
CEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCEEEEECCCC
PDAVFAANDMTALGCLYAFAQADVSVHDDVALAGFDDIPLARFVHPALTTMRVSIARLGE
CCHHHCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
QAMTRLMRLVDQPGHDDGIRQLLTPELVVRASCGAGEPGA
HHHHHHHHHHCCCCCCHHHHHHHCHHHEEEECCCCCCCCC
>Mature Secondary Structure 
AKGAVTIKDVAREARVSVATVSRALNGHDNVAGPVRQVVLDVAERLRYSPHAAARSLSS
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RRTQTLGVVLPDLYGEFFSELIRGIDGAARAAGQHLLVSSYHGDPEAQGRALRSMRGRVD
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCC
GLLVLSPYAEQPGFLTDNLPQALPTVLINAHLPGADYPVLSIDDHAGAMAMTEHLLAAGH
EEEEECCCCCCCCCCCCCHHHHHHHHEEECCCCCCCCCEEEECCCCCHHHHHHHHHHCCC
RRIAFIGGPALNFDARERLRGFRDAIAGHAGGAEGLEFAGDFDEASGHRAGLAMLAAGDL
CEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCEEEEECCCC
PDAVFAANDMTALGCLYAFAQADVSVHDDVALAGFDDIPLARFVHPALTTMRVSIARLGE
CCHHHCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
QAMTRLMRLVDQPGHDDGIRQLLTPELVVRASCGAGEPGA
HHHHHHHHHHCCCCCCHHHHHHHCHHHEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA