Click here to switch to the map view.

The map label for this gene is trmJ [H]

Identifier: 66768160

GI number: 66768160

Start: 2232763

End: 2233539

Strand: Direct

Name: trmJ [H]

Synonym: XC_1839

Alternate gene names: 66768160

Gene position: 2232763-2233539 (Clockwise)

Preceding gene: 66768158

Following gene: 66768161

Centisome position: 43.37

GC content: 68.98

Gene sequence:

>777_bases
ATGTCCGTCAGTCAGCGCATCCGTTTTGTTCTCGTCGGTACCCAGCATCCAGGCAACATCGGCGCCGCCGCGCGCGCGAT
GAAGACCATGGGGCTGACGCGCCTGGTGTTGGTCGCACCGGAAAAGGCACTCGATGAAGATGCCTACCGCCGCTCTGCTG
GCGCCGAAGACGTGCTCAGCGATGCACCGATCTTCGAGACGCTGGCCGAGGCGGTGGCGGACTGCACGCTGGTGATCGGC
TGCACGGCGCGCGCGCGCCGGGTAGCGCTGGAAGAGTTGGCGCCGGACGTGGGCGCGGGCCGCGCCTGGGCCAAGGCGGC
CGAACCGGCCGAGGTGGCGATCGTGTTCGGGCGCGAGCGCACCGGGCTGACCAATGACGAATTGCAGCTCTGCCATGCGG
CCGTGCATATCCCCTCCGACCCGCAGTTCAGTTCGCTCAACCTGGCTTCGGCGGTGCAGGTGCTGGCTTACGAGATGCGC
CTGGCACAGCTTGCGGCAGAGGCCACGCCCGCCGCACCGGCCGCGCCGGCCGCGGACAGCGCTGGCCCACGTGAGCTGCC
GGCCAGCCATGCGCAGATGGAAGGGTTGTTCGGCCAACTGGGCCAGACCCTGGACGAGATCGACTTCCATAAGGGCCGTG
CGCCGGATTCGGCAATGCGCAAGTTGCGTCGGCTGCTGCTGCGCGCGGAAATGACCGAGCAGGAAGTGCGTCTGCTGCGC
GGCATCCTGGCCGATGCCCAGCGCATGGCGGGGCTGGCGCGCCAGGCCGGAACCTGA

Upstream 100 bases:

>100_bases
AAGGCAGAATCGGAAATGGCGAAGGTAAAAGAGCGGTCCGGCGCGAAAGCCGGCTGCGCAGTTTACCATCTACGGTCGAA
CTGCTCCCCAATCTCCGTTC

Downstream 100 bases:

>100_bases
CGGCTGTCACAACCTGGAAGATTCGGCTAAGGTGTCGGCGACTCTAGGGGAGTAGCTGGTTTGCGCGGTCTACGATGCTG
TCTGATTGCGTGGCTGCTGG

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSVSQRIRFVLVGTQHPGNIGAAARAMKTMGLTRLVLVAPEKALDEDAYRRSAGAEDVLSDAPIFETLAEAVADCTLVIG
CTARARRVALEELAPDVGAGRAWAKAAEPAEVAIVFGRERTGLTNDELQLCHAAVHIPSDPQFSSLNLASAVQVLAYEMR
LAQLAAEATPAAPAAPAADSAGPRELPASHAQMEGLFGQLGQTLDEIDFHKGRAPDSAMRKLRRLLLRAEMTEQEVRLLR
GILADAQRMAGLARQAGT

Sequences:

>Translated_258_residues
MSVSQRIRFVLVGTQHPGNIGAAARAMKTMGLTRLVLVAPEKALDEDAYRRSAGAEDVLSDAPIFETLAEAVADCTLVIG
CTARARRVALEELAPDVGAGRAWAKAAEPAEVAIVFGRERTGLTNDELQLCHAAVHIPSDPQFSSLNLASAVQVLAYEMR
LAQLAAEATPAAPAAPAADSAGPRELPASHAQMEGLFGQLGQTLDEIDFHKGRAPDSAMRKLRRLLLRAEMTEQEVRLLR
GILADAQRMAGLARQAGT
>Mature_257_residues
SVSQRIRFVLVGTQHPGNIGAAARAMKTMGLTRLVLVAPEKALDEDAYRRSAGAEDVLSDAPIFETLAEAVADCTLVIGC
TARARRVALEELAPDVGAGRAWAKAAEPAEVAIVFGRERTGLTNDELQLCHAAVHIPSDPQFSSLNLASAVQVLAYEMRL
AQLAAEATPAAPAAPAADSAGPRELPASHAQMEGLFGQLGQTLDEIDFHKGRAPDSAMRKLRRLLLRAEMTEQEVRLLRG
ILADAQRMAGLARQAGT

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=245, Percent_Identity=46.530612244898, Blast_Score=191, Evalue=3e-50,
Organism=Escherichia coli, GI1790865, Length=159, Percent_Identity=37.1069182389937, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27422; Mature: 27291

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSQRIRFVLVGTQHPGNIGAAARAMKTMGLTRLVLVAPEKALDEDAYRRSAGAEDVLS
CCHHHEEEEEEEECCCCCCHHHHHHHHHHHCHHHEEEECCHHHHHHHHHHHHCCHHHHHC
DAPIFETLAEAVADCTLVIGCTARARRVALEELAPDVGAGRAWAKAAEPAEVAIVFGRER
CCHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEECCCC
TGLTNDELQLCHAAVHIPSDPQFSSLNLASAVQVLAYEMRLAQLAAEATPAAPAAPAADS
CCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
AGPRELPASHAQMEGLFGQLGQTLDEIDFHKGRAPDSAMRKLRRLLLRAEMTEQEVRLLR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
GILADAQRMAGLARQAGT
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVSQRIRFVLVGTQHPGNIGAAARAMKTMGLTRLVLVAPEKALDEDAYRRSAGAEDVLS
CHHHEEEEEEEECCCCCCHHHHHHHHHHHCHHHEEEECCHHHHHHHHHHHHCCHHHHHC
DAPIFETLAEAVADCTLVIGCTARARRVALEELAPDVGAGRAWAKAAEPAEVAIVFGRER
CCHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEECCCC
TGLTNDELQLCHAAVHIPSDPQFSSLNLASAVQVLAYEMRLAQLAAEATPAAPAAPAADS
CCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
AGPRELPASHAQMEGLFGQLGQTLDEIDFHKGRAPDSAMRKLRRLLLRAEMTEQEVRLLR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
GILADAQRMAGLARQAGT
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA